MACS® Technology pushing the boundaries

Fast and sensitive protein isolation

Flexible – from manual to automated, 96-well protocols

Sensitive isolation of epitope-tagged proteins – even for small samples

Accelerate your immunoprecipitation !

Easy capture of proteins via biotinylated molecules

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Translational research

• SensitivecDNAsynthesis,even for small samples

• Fastandconvenient protein isolation

MACSmolecular

• Powerfulandcompactflow cytometers

•UniqueVio®Dyes

• Sensitiveanalysisof low-frequencycells

MACS® Flow Cytometry

• High-qualitycytokines up to GMP grade

• Specializedmedia

• Peptidepoolsforefficientcell stimulation

MACS® Cell Culture

• Twoversatileplatformsfor cell processing and cell separation

• Reliableandreproducibleresults through standardizedprocesses

• Functionallyclosed, sterile systems

CliniMACS® Systems

• ContrastreagentsforMRI,CT,ultrasound,andopticalimaging

• Specificallycreatedforsmall animal imaging

Viscover™ Imaging Agents

• Gentleyeteffectivetissuedissociation

• Cellsurfaceepitopesconserved

• Standardizedprocedures,independent of userMACS® Sample

Preparation

ClinicalResearch

• CryoMACSFreezingBags,for cryopreservation applications at ultra-low temperature

• Uniquebagconceptfor safe cryopreservation

• CryoMACSDMSO10,used as cryoprotective agent

CryoMACS® Freezing Products

• Cellularproductsmanufactured according to GMP guidelines

• CliniMACSSystemsoptimizedforcombinationwith MACS GMP Products

• Over14yearsofexperiencein GMP manufacturing

MACS® GMP Products

• Premiumrecoveryof cell subpopulations

• Gentleisolationofviableand functional cells

• ThemostcitedseparationtechnologyMACS® Cell Separation

MACS® Technology by Miltenyi BiotecComprehensive solutions from cells to molecular analysis

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Products for fast and sensitive protein isolationProteomic analyses are conducted in many research fields to gain a better understanding of particular biological processes and play a major role in drug screening and target identification procedures.

Miltenyi Biotec offers a wide variety of products based on the renownedMACS®Technologythatisolateproteinsandtheirinteractingpartners.MACSTechnologyforproteinisolationissimple, straightforward, and takes you from cell or tissue sample topureproteininlessthantwohours.Thehighrateofrecoveryand extraordinary sensitivity is achieved with superparamagnetic MACSMicroBeads.These50-nmparticlesinstantlybindtotheirtargets and allow protein isolation even from small amounts of startingmaterialorwhenworkingwithrareproteins.Thelowrate of background binding of MACS MicroBeads and the usage of proven binding moities, such as monoclonal antibodies, protein A or G, or streptavidin enables highly specific isolation of target proteins.

One technology for several applications • Isolationofepitope-taggedproteins

• (Co)-Immunoprecipitationsofproteins

• Isolationsofproteinsviabiotinylatedcaptureprobes

Manual to automated – it’s your choice !Choose between a manual procedure with 1 – 4 samples in parallel using the µMACS™ Separator, or use the semi-automated approachwithupto96samplesbyutilizingtheMultiMACS™M96 Separator. Fully automated approaches up to 96 samples are easily set up by integrating the MultiMACS M96 Separator in a robotic system.

Caught your attention ?Have a look at the following pages and learn how MACS Technologyhasthepotentialtoadvanceyourproteinresearch.

Contents

4 How MACS® Technology works Toisolateproteinsquicklyandsensitively

6 HA•His•c-myc•GFP•GST•DYKDDDDK Manual to automated isolation of tagged proteins

8 ImmunoprecipitationandChIP-in-a-column From single-sample to automated, 96-well processes

10 Isolation of proteins via biotinylated capture probes From single-sample to automated, 96-well processes

11 Isolation of native transcription factors

12 Product overview

15 References Selected from more than 20,000 papers using products from Miltenyi Biotec

MACSmolecular products MACS® Technology for molecular applications

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Lysis of cells and removal of cell debris

Labeling of target proteins withµMACS™Anti-TagMicroBeads

Application of labeled cell lysate toMACS®ColumnplacedinaMACS Separator

Removal of unbound material by stringent washing

Optional:enzymaticreaction in the column

Elution of target protein while MicroBeads remain in column

Total time: 1 h 32 min

Proteins and complexes

Epitope-tagged protein

Anti-TagMicroBead

Figure 1: PrincipleofMACSTechnologyforisolationofepitope-taggedproteins.

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The operating principle Superparamagnetic µMACS™ MicroBeads are applied to the cell lysateandinstantlybindtotheirtargetprotein.Themagneticallylabeled protein is then isolated and purified in a MACS Column positioned within the magnetic field of a MACS Separator. After thorough washing, pure protein is eluted.

How you benefit from MACS® Technology for protein isolation

High sensitivity Even rare proteins can be isolated.

High Speed Less than two hours from cells or tissue to pure protein.

High specificityLow background binding.

High recovery Nolossofmaterialascentrifugationstepsandbufferremovalare rendered unncessary.

Reproducibility and reliabilityProtein isolation procedures can be easily automated.

Did you know?

Insteadofelutingtheprotein,enzymaticreactions such as kinase assays can be performed directly in the column. For incubation at elevated temperatures, the thermoMACS™ and MultiMACS™ M96thermo Separators are available.

How MACS® Technology works

To isolate proteins quickly and sensitively

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The components

1. µMACS™ MicroBeads • Superparamagnetic–onlymagnetizedina magnetic field

• Smallinsize–just50nmindiameter

• Non-sedimentingwithextremelyhighreaction kinetics – instantly bind target protein

• Lownon-specificbinding

Benefit: High sensitivity and specificity – even rare proteins can be efficiently isolated while enrichment of non-specific proteins is minimal.

2. MACS Column• Packedwithsteelspheresthatenhancethemagnetic field–essentialtoretainnanometer-sizedµMACS™ MicroBeads bound to target protein

• Buffersrunbygravityflow,sothereisnoneedfor centrifugation or buffer removal, thus preventing a loss of target

• Thoroughrinsingprocedure

Benefit: High recovery and purity.

3. MACS SeparatorsTheµMACS Separator is a permanent magnet for manual processing of up to four samples in parallel. For higher throughput experiments, the procedure can easily be scaled up toa96-wellformatutilizingtheMultiMACS M96 Separator. A fully automated process is achieved by integrating this benchtop instrument into a robotic pipetting system.

Benefit: High level of reproducibility and reliability.

Figure 2: IsolateGFPfusionproteinsfromavarietyofsourceswiththeµMACS Anti-GFP MicroBeads

Figure 3: MACS Columns enable fast and sensitive separations.

Figure 5: µMACS SeparatorFigure 4: MultiMACS M96 Separator

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Isolation of epitope-taggedproteinsTheμMACS Tag Isolation Kits contain μMACS MicroBeads coupledtohigh-qualitymonoclonalantibodiesspecificforproteins tagged with:

• HA(hemagglutinin)

• His(histidine-epitope)

• DYKDDDDK(alsoknownasFLAG®tag)

• c-myc

• GFP(greenfluorescentprotein)

• GST(glutathioneS-transferase)

TheseμMACSAnti-TagMicroBeadsareoptimizedforthespecific and sensitive isolation of recombinant epitope-tagged proteins. Particularly when working with eukaryotic cells, sensitive isolation with low rate of background binding is essential.

Benefits of MACS Technology for recombinant protein isolation

High specificitySpecificmonoclonalantibodiestargetthetagsequence and ensure pure protein eluates.

High sensitivityDuetothefastreactionkineticsofµMACSAnti-TagMicroBeads,important for the isolation of rare proteins or when working with limited starting material.

High speedNoneedfortime-consumingpre-clearingandcentrifugation.Proteins are isolated in about 90 minutes.

Figure 6: Specific isolation of recombinant fusion protein with μMACS Anti-c-myc MicroBeads Cells were transfected with a vector encoding c-myc-BDCA-2andlabeledwith35S-methionine.Therecombinant c-myc-BDCA-2proteinwaspurifiedwithμMACSAnti-c-mycMicroBeads.Leftlane:controlpurificationwithAnti-HAMicroBeads.Thefigureshows anautoradiogramafterSDS-PAGE.

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Figure 7: Sensitive isolation of recombinant fusion protein with μMACS Anti-HA MicroBeads 10⁷mousepre-Bcells(1881)weretransfectedwithavectorencodingHA-taggedBDCA-2,andproteinisolationwasperformedusingcellpopulationswitheither1%(lane1,3)or10%(lane2,4)positivelytransfectedcells.Wholecelllysates(lane1,2)or20%oftheproteinisolationeluatewithμMACSAnti-HAMicroBeads(lane3,4)wereseparatedbySDS-PAGE,blottedonamembrane,anddetectedbyusing an Anti-HA-HRP antibody.

HA • His • c-myc • GFP • GST • DYKDDDDKManual to automated isolation of tagged proteins

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Automated96-wellisolationofepitope-taggedproteinsinless than two hoursTheMultiMACS™ Tag Isolation Kits enable researchers tomagneticallyisolatec-myc-,GFP-,GST-,HA-,His-,orDYKDDDDK-taggedproteinsinahigherthroughputprocedure.

Up to 96 samples can be processed in parallel with the compact, benchtop MultiMACS M96 Separator, either manually or fully automatically when integrated in a pipetting roboter.

Analysisofepitope-taggedproteinsMonoclonal Anti-c-myc, Anti-GFP, Anti-HA, Anti-His, orAnti-DYKDDDDK-taggedantibodiesareavailablewithseveral conjugates:

• Biotin

• HRP

• FITC

• PE

Fluorochrome-conjugatedAnti-Tagantibodiesallowimmunofluorescence analysis by flow cytometry or fluorescence microscopy. Biotinylated antibodies can be used in combination with streptavidin conjugates, such as streptavidin-HRP, in Western blot detection, or with fluorochrome-streptavidin for immunofluorescence analysis.

Directlycoupledtohorseradishperoxidase(HRP),theantibodiessimplifyWesternblotorELISAanalysisbecauseincubations with secondary antibodies are not necessary.

The antibodies are ideally suited for • flowcytometry

• fluorescencemicroscopy

• immunoblotting/Westernblotting

• ELISA

Figure 9: Sensitive detection of recombinant fusion protein with Anti-GFP-HRP antibody20ng,10ng,5ng,and1ngGFPfusionproteinwereseparatedbySDS-PAGEandblottedonPVDFmembrane.GFPfusionproteinsweredetectedwithAnti-GFP-HRPantibody(1:5,000,1hour,roomtemperature)andECLreagent(GEHealthcare).

Figure 8: MultiMACS M96 Separator integrated in pipetting robot for fully automated protein isolation in a 96-well format.

HA • His • c-myc • GFP • GST • DYKDDDDKManual to automated isolation of tagged proteins

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Immunoprecipitation and ChIP-in-a-columnFrom single-sample to automated, 96-well processes

Immunoprecipitation with μMACS™ Protein A/G MicroBeads TheμMACS™ Protein A/G MicroBeads were developed forsmall-scaleanalyticimmunoprecipitation(IP).The extremelysmallμMACSProteinA/GMicroBeadsensureveryfast reaction kinetics: Formation of the labeled immune complexisgenerallycompletedin30minutes.There’sno need for overnight incubation.

High sensitivityDuetothesmallsizeofμMACSMicroBeads,bindingtotargetproteins is extremely fast and efficient; higher amounts of target protein can thus be captured per sample.

High speedMACSTechnologysavestime:Theexperimentcanbecompletedwithin2hours,whileconventionalIPmayrequireup to one day of work.

High specificity Theminimizednon-specificbindingofμMACSProteinA/GMicroBeads and the efficient and gentle washing in the columnsignificantlyreducebackgroundbinding.Thewashingprocedurecanbeoptimizedforanytargetmolecule,andevenfragileproteincomplexescanbesuccessfullyisolatedbyco-IPwithMACSTechnology.

Figure 11: Co-immunoprecipitation of Beta-Catenin Androgen receptor wasimmunoprecipitatedfromdihydrotestosterone(DHT)-stimulated(lanes1,3)orunstimulated(lanes2,4)LNCaPcellswithµMACSProteinGMicroBeads(lanes1,2)orwithProteinA/Gagarosebeads(lanes3,4).Westernblotusinganti-beta-cateninantibodyshowsbeta-catenin(BCat)co-immunoprecipitatedwithandrogenreceptor.(CourtesyofD.Mulholland, Vancouver,Canada)

Figure 10:ImmunoprecipitationoftheSV40largeTantigenImmunoprecipitationwasperformedfromCOS-7cellsusingμMACS ProteinGMicroBeads.Coomassie-stainedgradientSDS-PAGEofaproteinmarker(lane1),theflowthrough(lane2),thewashfraction(lane4), theimmuno-precipitatedlargeTantigen(lane6,indicatedbythearrow),andanisotype-matchedcontrolantibody(lane5).

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Immunoprecipitation and ChIP-in-a-columnFrom single-sample to automated, 96-well processes

ChIP-in-a-columnwith MACS TechnologyTheμMACS Protein A/G MicroBeads improve standard immunoprecipitation and significantly accelerate the search for interactingproteins.Chromatinimmunoprecipitation(ChIP)protocols also benefit from the higher specificity and lower backgroundbindingofμMACSProteinA/GMicroBeads.

Automated96-well immunoprecipitation or ChIPTheMultiMACS M96 Separator allows the parallel processing of up to 96 samples with Multi-8 or Multi-96 Columns. Thiscompactbenchtopinstrumentallowssemi-automatedor – in combination with a robotic pipetting system – fully-automated magnetic isolation of molecules.

BothimmunoprecipitationandChIPcaneasilybeupgradedforthe automated processing of up to 96 samples in parallel using the MultiMACS Protein A/G Kits.

Figure 12:Chromatinimmunoprecipitation(ChIP)PCR reaction of theprostate-specificantigen(PSA)geneusingDNAobtainedbyChIP fromculturedcells.Immunoprecipitationwascarriedoutwith anti-androgen-receptorantibodyandµMACSProteinGMicroBeads(lanes1,2) orwithProteinA/Gagarosebeads(lanes3,4).Dihydrotestosterone (DHT)-stimulated(lanes1,3)orunstimulated(lanes2,4)cellswereused forChIP.(CourtesyofD.Mulholland,Vancouver,Canada)

Figure 13: IsolationofmousePD-1proteinwithMultiMACSProteinGMicroBeads Cell lysates of 106CHOcells,lane3,andCHOcellsexpressingc-myc-taggedmousePD-1taggedwithc-myc,lane1,2,4–8,wereimmunopurified using an anti-c-myc horseradish peroxidase conjugate andMultiMACSProteinGMicroBeadsonaMulti-8Columnstrip.PurifiedeluateswereanalyzedviaSDSPAGEandsubsequentimmunoblottingusinganti-c-myc antibodies.

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Isolation of proteins via biotinylated capture probes

From single-sample to automated, 96-well processes

Isolation of biotinylated moleculesTheμMACS Streptavidin Kit specifically isolates of any molecule interacting with a biotinylated capture probe – also veryusefulforsearchingandanalyzingbindingpartnersofbiotinylated proteins; for example, receptor ligands or signallingactivators.Theprinciplealsoworksfornucleic acids,suchasmRNAorviralsequences.Evenlargemolecularcomplexes, organelles, or viable viruses can be purified with MACSTechnology.

ApplicationsfortheμMACSStreptavidinKit• Detectionandanalysisof: - protein-protein interaction -DNA-proteininteraction -RNA-proteininteraction

• Immunoprecipitationusingbiotinylated antibodies

• Isolationofspecifictranscripts

• IsolationofmicroRNAs

• IsolationoftRNAmolecules

• Isolationofribozymes

• Virusisolation

• Serialanalysisofgeneexpression(SAGE)

• Subtractivehybridization

• Phageandyeastdisplay

Protocols for each application are available at www.miltenyibiotec.com.

Figure 15:IsolationofspecificRNAbindingproteinsYeastcrudeextractwaspre-clearedandsubsequentlyincubatedwithafull-lengthMatingFactorA2mRNAboundtoa3'-biotinylatedcomplementarysingle-strandedoligonucleotide and magnetically labeled with µMACS Streptavidin MicroBeads.Thefigure(A)showsthesilver-stainedSDSgel.Fourproteinswithmolecularweightsof33,44,48,and51kDa,whichbindspecificallytotheRNAsequence,wereisolated(eluate).AsacontrolamagneticallylabeledmutantmRNA,lackingthebindingsiteforMatingFactorA2bindingproteins,wasused.Inthecontrolexperiment(B)nospecificproteinswereisolated.(CourtesyofDr.A.Albig,WashingtonStateUniversity,USA)

Figure 14: PurificationofNanRLanes show Coomassie-stained samples fractionatedby4–20%SDS-PAGE.Lane1:BenchmarkPrestainedMarkers(Invitrogen);lane2:solubleproteinfractionfromJM109harboringpSX675after inductionwith0.2%L-arabinose(30.9μg);lane3:purifiedrecombinantNanR(3.5μg);lane4:NanRfromE. coliMC4100(1.68μg).

A B

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Isolation of proteins via biotinylated capture probes

From single-sample to automated, 96-well processes

Automated96-wellisolation of biotinylated probesTheMultiMACS Streptavidin Kits in combination with the MultiMACS M96 Separator simultaneously isolate up to 96 samples in a semi-automated or – in combination with a robotic pipetting system – fully automated process.

Isolation of native transcription factors TheμMACS FactorFinder Kit performs native isolation of DNA-bindingproteinsandisthereforewell-suitedtodetectandanalyzenoveltranscriptionfactors.

Theapplicationsofthekitareasdiverseasthequestionsintranscriptionalregulationresearch:Individualtranscriptionfactorsandtheirco-factorscanbedetectedandcharacterized;DNA-proteininteractionsandtarget-sequencescanbeanalyzed. Thekitcanalsobeusedtoinvestigatetranscriptionfactors as potential therapeutic targets.

FeaturesoftheμMACSFactorFinderKit• Uniqueready-to-usekit

• One-stepextractionofcytosolicandnuclear fraction with special Lysis Buffer

• Lessthan90minutesrequiredfortheisolation of functional transcription factors

• Bothnativeordenaturingelutionarefeasible, depending on downstream application

Figure 16: IsolationofHA-taggedproteinswithMultiMACSStreptavidinMicroBeads 100 μL lysates of non-transfected E. coli (controllysate)andE. colitransfectedwithanexpressionvectorforHA-taggedprotein(testlysate)were incubated with 2 μg anti-HA-Biotin and 100 μL of Streptavidin MicroBeads for 30 minutes on ice.The proteins were then purified with aMultiMACSM96SeparatorandanalyzedonanSDSgelstainedwithCoomassie Brilliant Blue. Lane 1: molecular weight marker; lane 2: E. coli control lysate; lane 3: transfected E. coli lysate; lanes 4, 6, 8, 10: purified test lysate; lanes 5, 7, 9, 11: purified control lysate.

Figure 17: IsolationofphosphorylatedSTAT-3(pSTAT-3)from activatedTcellsTcellsfromhumanPBMCswereactivatedandexpandedinthepresenceofIL-2usingtheTCellActivation/ExpansionKit(#130-091-441).Afterthreedays,cellswereincubatedwithIL-15for15minutes,washed, and lysed with Cell Lysis Buffer. Whole cell lysate was incubated withabiotinylated31-bpDNAprobecomprisingtheSTAT-3bindingsequence.µMACSStreptavidinMicroBeadswereadded,andmagneticisolationwasperformed.ThetranscriptionfactorwaselutedwithhighsaltElutionBuffer.TheWesternblot(usingaphosphospecificSTAT-3antibody)showsahighamountofpSTAT-3intheeluate.L:celllysate;FT:flow-through; W1, W2: wash fractions; E: eluate.

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Product overview

Products Capacity Order no.

Isolation of epitope-tagged proteins

µMACSc-mycIsolationKit¹ 40 isolations 130-091-123

µMACSAnti-c-mycStartingKit² 40 isolations 130-091-284

µMACSHisIsolationKit¹ 40 isolations 130-091-124

µMACSAnti-HisStartingKit² 40 isolations 130-091-285

µMACSHAIsolationKit¹ 40 isolations 130-091-122

µMACSAnti-HAStartingKit² 40 isolations 130-091-286

µMACSGFPIsolationKit¹ 40 isolations 130-091-125

µMACSAnti-GFPStartingKit² 40 isolations 130-091-288

µMACSGSTIsolationKit¹ 40 isolations 130-091-370

µMACSAnti-GSTStartingKit² 40 isolations 130-091-493

µMACSDYKDDDDKIsolationKit¹ 40 isolations 130-101-591

µMACSAnti-DYKDDDDKStartingKit² 40 isolations 130-101-636

ProCatch His Resin 10 mL 130-092-184

ProCatch His Resin 25 mL 130-092-183

ProCatch His Resin 100 mL 130-092-182

ProCatch Glutathione Resin 10 mL 130-092-187

ProCatch Glutathione Resin 25 mL 130-092-186

ProCatch Glutathione Resin 100 mL 130-092-185

96-well isolation of epitope-tagged proteins

MultiMACSc-mycIsolationKit(12×8)³ 96 isolations 130-094-250

MultiMACSc-mycIsolationKit(4×96)⁴ 384 isolations 130-094-251

MultiMACSGFPIsolationKit(12×8)³ 96 isolations 130-094-252

MultiMACSGFPIsolationKit(4×96)⁴ 384 isolations 130-094-253

MultiMACSGSTIsolationKit(12×8)³ 96 isolations 130-094-254

MultiMACSGSTIsolationKit(4×96)⁴ 384 isolations 130-094-256

MultiMACSHAIsolationKit(12×8)³ 96 isolations 130-094-255

MultiMACSHAIsolationKit(4×96)⁴ 384 isolations 130-094-257

MultiMACSHisIsolationKit(12×8)³ 96 isolations 130-094-258

MultiMACSHisIsolationKit(4×96)⁴ 384 isolations 130-094-259

MultiMACSDYKDDDDKIsolationKit(12×8)³ 96 isolations 130-101-621

MultiMACSDYKDDDDKIsolationKit(4×96)⁴ 384 isolations 130-101-623

1) Kitcontains2mLAnti-TagMicroBeads,LysisBuffer,WashBuffer,ElutionBuffer. 2) StartingKitcontains1µMACSTagIsolationKit,1µMACSSeparator,1MACSMultiStand,2×20µColumns.3) Kitcontains3×2mLµMACSAnti-TagMicroBeads,EquilibrationBuffer,12×Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate.4) Kitcontains5×4.6mLµMACSAnti-TagMicroBeads,EquilibrationBuffer,4×Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.

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Products Contents / Components Capacity Order no.

Detection of epitope-tagged proteins

Anti-c-myc-Biotin 1 mL up to 100 tests 130-092-471

Anti-c-myc-FITC 1 mL up to 100 tests 130-092-472

Anti-c-myc-HRP 100 μL, suggested dilution range 1:1,000 –1:10,000 130-092-113

Anti-His-Biotin 1 mL up to 100 tests 130-092-692

Anti-His-FITC 1 mL up to 100 tests 130-092-675

Anti-His-HRP 100 μL, suggested dilution range 1:1,000 –1:10,000 130-092-785

Anti-His-HRP(C-terminal) 100 μL, suggested dilution range 1:1,000 –1:10,000 130-092-783

Anti-His-PE 1 mL up to 100 tests 130-092-691

Anti-HA-Biotin 1 mL up to 100 tests 130-092-258

Anti-HA-FITC 1 mL up to 100 tests 130-092-256

Anti-HA-HRP 100 μL, suggested dilution range 1:1,000 –1:10,000 130-091-972

Anti-HA-PE 1 mL up to 100 tests 130-092-257

Anti-GFP-HRP 100 μL, suggested dilution range 1:1,000 –1:10,000 130-091-833

Anti-DYKDDDDK-PE 1 mL up to 100 tests 130-101-576

Anti-DYKDDDDK-APC 1 mL up to 100 tests 130-101-564

Anti-DYKDDDDK-HRP 100 µL, suggested dilution range: 1:1,000 –1:10,000 130-101-572

Immunoprecipitation and ChIP

μMACS Protein A MicroBeads 2 mL 40 isolations 130-071-001

μMACS Protein G MicroBeads 2 mL 40 isolations 130-071-101

μMACSProteinA/GStartingKit 2 mL μMACS Protein A or G MicroBeads, 1 μMACS Separator, 20 μ Columns, 1 MACS Multistand

40 isolations 130-042-601

MultiMACSProteinAKit(24×8) 5×2mLμMACSProteinAMicroBeads;24Multi-8Columns; 2MultiColumnFrames;2DeepWellBlocks,2.5mL, with sealing foil; 2 Microtiter Plates, U-bottom

192 isolations 130-092-944

MultiMACSProteinGKit(24×8) 5×2mLμMACSProteinGMicroBeads;24Multi-8Columns; 2MultiColumnFrames;2DeepWellBlocks,2.5mL, with sealing foil; 2 Microtiter Plates, U-bottom

192 isolations 130-092-946

MultiMACSProteinAKit(4×96) 10×2mLμMACSProteinAMicroBeads;4Multi-96ColumnswithMultiColumnFrames;4DeepWellBlocks,2.5mL, with sealing foil; 4 Microtiter Plates, U-bottom

384 isolations 130-092-945

MultiMACSProteinGKit(4×96) 10×2mLμMACSProteinGMicroBeads;4Multi-96ColumnswithMultiColumnFrames;4DeepWellBlocks,2.5mL, with sealing foil; 4 Microtiter Plates, U-bottom

384 isolations 130-092-947

Support is just a phone call away

Fortechnicalquestionsandestablishmentofnewrotocols,pleasecontactMiltenyiBiotec’sTechnicalSupportTeam.Experiencedscientistsofferadvicerangingfrom experimental design and sample preparation to automateduseofMACSTechnologyforproteinisolation.

For country-specific contact data, please refer to www.miltenyibiotec.com/support

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Products Contents / Components Capacity Order no.

Isolation via biotinylated molecules or isolation of transcription factors

μMACSStreptavidinKit¹ 20 isolations 130-074-101

μMACSStreptavidinStartingKit² 20 isolations 130-091-287

MultiMACSStreptavidinKit(12×8)³ 96 isolations 130-092-948

MultiMACSStreptavidinKit(4×96)⁴ 384 isolations 130-092-949

μMACSFactorFinderKit⁵ 20 isolations 130-092-317

μMACSFactorFinderStartingKit⁶ 20 isolations 130-092-318

MACS Separators and MACS Columns for molecular biology

μMACS Separation Unit Compatible with μ Columns 130-042-602

thermoMACS Separation Unit Compatible with μ Columns 130-091-136

MiniMACS Separation Unit Compatible with M Columns 130-042-102

OctoMACS Separation Unit Compatible with M Columns 130-042-109

MultiMACS M96 Separator Compatible with Multi-8 and Multi-96 Columns 130-091-937

MultiMACS M96thermo Separator Compatible with Multi-8 and Multi-96 Columns 130-094-534

μ Column 20 columns 130-042-701

M Column 10 columns 130-042-801

Multi-8Columns,molecular(12×8) 12Multi-8Columns,1MultiColumnFrame,1DeepWellBlock, 1 Microtiter Plate

130-092-444

Multi-96Column,molecular(4×96) 4 Multi-96 Columns with MultiColumn Frames, 4DeepWellBlocks,4MicrotiterPlates

130-092-445

1) Kitcontains2mLμMACSStreptavidinMicroBeads,EquilibrationBuffers,20μColumns.2) Kitcontains1μMACSStreptavidinKit,1μMACSSeparator,1MACSMultistand.3) Kitcontains5×2mLμMACSStreptavidinMicroBeads,EquilibrationBuffers,12Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate.4) Kitcontains20×2mLμMACSStreptavidinMicroBeads,EquilibrationBuffers,4Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.5) Kitcontains2mLμMACSStreptavidinMicroBeads,Lysis,Binding,Wash,andElutionBuffers,BindingEnhancer,20μColumns.6) Kitcontains1μMACSFactorFinderKit,1μMACSSeparator,1MACSMultistand.

Product overview

ReferencesNearly 20,000 publications to date using MACS Technology

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Anti-c-mycIsolationKitsStaubach et al. (2009)Proteomics9:2820–2835.Robert et al. (2006)J.Biol.Chem.28:40135–40143.Spoelgen et al. (2006)J.Neurosci.2:418–428.Mach et al. (2005)J.Virol.79:2160–2170.

Anti-GFPIsolationKitsGreaves et al. (2009)Mol.Biol.Cell20:1845–1854.Fujimura et al. (2008)RNA14:425–431.Boulon et al. (2008)JCellBiol.180:579–595.Gorchakov et al. (2008)JVirol.82:10088–10101.Galvani et al. (2008)Mol.Biol.Cell28:3672–3685.Sullivan et al. (2008)Mol.Plant1:178–194.Palma et al. (2007)GenesDev.21:1484–1493.vonKnethenet al. (2007)J.CellBiol.176:681–694.Cole et al. (2006)NucleicAcidsRes.4:1281–1292.Frolova et al. (2006)J.Virol.8:4122–4134.Koppet al. (2006)Mol.Biol.Cell17:2811–2823.Ossenbuehl et al. (2006)PlantCell18:2236–2246.Spoelgen et al. (2006)J.Neurosci.2:418–428.

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