luna - instituto de qu mica - universidad de...
TRANSCRIPT
Phenomenex 143
HPLC
App
ID 1
100
LUNA®
*Luna CN is stable from pH 1.5 to 7.0, Luna Amino (NH2) is stable from pH 1.5 to 11.0,Luna SCX is stable from pH 2.0 to 7.0.**The comparative data presented here may not be representative for all applications.
Comparison of 7 different 5µ reversed phase columns. Thissurvey measures the degree of silanol activity on the surfaceof each silica. In this survey, Luna 5µ C18(2) material demon-strates the lowest silanol activity.
One of the most popular HPLC columnsworldwide• pH stability from 1.5 to 10.0* for over 1000 hours• Extensive batch traceability data provided with
every 3µ and 5µ column• Extreme versatility of 3, 5, 10, 10-PREP and 15µ particles
and bulk material availability• Phases available include: Si, C5, C8, C18,
Phenyl-Hexyl, CN, NH2 and SCX
Sharp Peak Shape
Luna® is manufactured with remarkable sil ica, dense bondedphase coverage and exhaustive endcapping to improve peakshape over a broad pH range for accurate, sensitive meth-ods. Free exposed silanols contribute significantly to peaktailing. Incomplete or lack of endcapping exposes free silanolsto the analytes, and additionally metal contamination wil l in-crease the acidity of the free si lanols, leaving them in acharged state even at low pH. Using a simple 50:50 water/acetonitri le mobile phase, the peak shapes and separationof pyridine and phenol provides a good indicator of freesilanols (see results below). Under these conditions, in thepresence of ionized exposed silanols, pyridine elutes as atail ing peak, typically coeluting or even eluting after phenol.
Luna
Pyridine Peak Asymmetry Comparison**
Material CharacteristicsPacking Particle Pore Surface Carbon Calculated BondedMaterial Shape/Size Size Area Load Phase Coverage
(µ) (Å) (m2/g) % (µmole/m2)Luna Silica(2) Spher. 3, 5,10,15 100 400 0 –Luna C5 Spher. 5,10 100 440 12.5 7.85Luna C8 Spher. 5,10 100 440 14.75 5.50Luna C8(2) Spher. 3, 5,10,15 100 400 13.5 5.50Luna C18 Spher. 5,10 100 440 19 3.00Luna C18(2) Spher. 3, 5,10,15 100 400 17.5 3.00Luna Phenyl-Hexyl Spher. 3, 5,10, 15 100 400 17.5 4.00Luna CN* Spher. 3, 5, 10 100 400 7.0 3.80Luna NH2* Spher. 3, 5, 10 100 400 9.5 5.80Luna SCX* Spher. 5,10 100 400 0.15 meq/g
1
2
3Pyridine Asymmetry:As 2.27
0 2 4 6 8 10 12 min
0
100
200
300
1
2
3
Pyridine Adsorption:No elution
0 2 4 6 8 10 12 min
0
100
200
300
400
500
1
2
3
0 2 4 6 8 10 12 min
0
100
200
300
400 Pyridine Asymmetry:As 1.44
Zorbax® 5µ C18
Symmetry® 5µ C18
LUNA® 5µ C18(2)
Peak Asymmetry Comparison of Competing Columns**
Conditions for all columns
Dimensions: 150 x 4.6mmMobile Phase: Acetonitrile/Water (50:50)Flow rate: 1.0mL/minDetection: UV @ 254nmSample: 1. Pyridine
2. Phenol3. Toluene
Phenomenex144
HPLC
LUNA®
Remarkable Silica Technology
Luna’s base sil ica is manufactured to extremely tight toler-ances. Not only is it 99.999% ultrapure, but it has excellentsurface smoothness and sphericity. The structural stabil ityof individual sil ica particles and stabil ity of the column bedare enhanced by improved sil ica smoothness. The strengthof Luna sil ica reduces the likelihood of particle sheering andbreakage during bonding and everyday column use, thusreducing the number of “fines”, lowering backpressure, andlengthening column life.
Dense Uniform Bonding
Luna columns employ bonding technology that provides ex-tremely high surface coverage not only through high-bondingdensities, but also controlled bonding uniformity. This highdegree of uniformity forms a more inert “hydrophobic shield”around the underlying silica. The result is a pH stable, repro-ducible bonding with virtually no silanol activity.
Scale-up Diversity
Luna is available in 3, 5, 10, 10-PREP and 15µ particle sizesas packed columns or bulk media for fast, simple scale up.Your methods will have scale-up flexibility without the need toredevelop on another sorbent.
Quality Proven
Extensive testing and quality control test data is shownon the Materials Validation Document (MVD) that accom-panies each 3 and 5µ Luna column. Over 25 individualchemical and physical testes are performed and docu-mented on the MVD, including:
• Particle Analysis- Particle Size- Pore Diameter- Particle Size Distribution- Surface Area
• Total Metal Content• Bonded Phase Coverage
- Total Carbon- Surface Coverage
• Diagnostic Chromatography Test- Inertness- Metal Sensitivity- Hydrophobic Index
• Performance Chromatography Test- Basic Drug Standards- Longevity Tests (pH Stability)- Scanning Electron Microscopy (measures
surface smoothness and particle shape)
Luna
Compare Surface Smoothness
Phe
nom
enex
Lun
a®
5µ C
18W
ater
s S
ymm
etry
®
5µ C
18A
gile
nt T
echn
olog
ies
Zorb
ax®
5µ S
B-C
18
Phenomenex 145
HPLC
Wide pH Stability
Luna’s 1.5 to 10 pH stability** is a result of manufacturing highpurity silica that is nearly free of metal contaminates. Metalcontaminants hinder maximum bonded phase coverage of thesilica surface, which is necessary for achieving a broad pHstability range. Our bonding technology ensures the bondedphase coverage is extraordinarily dense creating a “hydropho-bic shield” around the silica.
pH Stability Data for Luna 5µ C18(2)
KEY:
Efficiency, Plates for Toluene
tR of Toluene (min)
k of Toluene
Symmetry of Toluene
Symmetry of Pyridine�
** Luna CN is stable from pH 1.5 to 7.0Luna NH2 is stable from pH 1.5 to 11.0Luna SCX is stable from pH 2.0 to 7.0.
LUNA®
Luna
Test Conditions: Column stability tested under highly acidic conditions.Continuous flush in 0.1% TFA (pH 1.5) in Water/Acetonitrile, 50:50.
Test Conditions: Column stability tested under highly basic conditions.Continuous flush in 20mM Na2HPO4 (pH 10.0) in Water/Acetonitrile, 50:50.
If Luna does not provide at least anequivalent separation as compared to acompeting column of the same particlesize, similar phase and dimensions, sendin your comparative data within 45 daysand keep the Luna column for FREE.
Phenomenex146
HPLC
1,2,3
4
5
1 2 3 4 5 min
0
200
400
600
800
1000
1200
1400
App ID 12909App ID 12908
App ID 12911
App ID 12967
App ID 12904
App ID 12906
App ID 12903
LUNA®
*The comparative data presented here may not be representative for all applications.
1
2
3
4
5
2 4 6 8 min
0
100
200
300
400
500
600
700
1
2
3
4
5
5 10 15 min
0
50
100
150
200
Agilent TechnologiesZorbax® 5µ SB-C8
Tricyclic Antidepressants
Conditions for all columns:Dimensions: 150 x 4.6mmMobile Phase: 10mM KH2PO4, pH7.0/Acetonitrile/
Methanol (16:74:10)Flow rate: 1.0mL/minDetection: UV @ 210nmSample: 1. Desipramine
2. Nortriptyline3. Doxepin4. Imipramine5. Amitriptyline
LUNA® 5µ C8 Waters Symmetry® 5µ C8 Waters SymmetryShield™
5µ RP8
Agilent TechnologiesZorbax® 5µ XDB-C8
Anti-Inflammatory/Analgesic Drugs
Conditions for all columns:Dimensions: 75 x 4.6mmMobile Phase: 20mM KH2PO4, pH 6.0/Acetonitrile (70:30)Flow rate: 0.75mL/minDetection: UV @ 202nmSample: 1. Tolmetin 4. Fenoprofen
2. Naproxen 5. Indomethacin3. Diflunisal 6. Ibuprofen
Waters Symmetry® 3.5µ C18
Agilent Technologies Zorbax® 3.5µSB-C18
12
3
4
56
0
25
50
75
100
125
150
175
0 5 10 15 20 min
1
2
3
4
5
6
0
25
50
75
100
125
150
175
0 5 10 15 20 min
LUNA® 3µC18(2)
App ID 12910
Chromatographic Comparisons of C18 Columns*
Chromatographic Comparisons of C8 Columns*
Luna
1 2 3 4 5 mi n
1
2 5
4
0
200
400
600
800
1000
3
Phenomenex 147
HPLC
App
ID 1
0822
App
ID 1
0821
App
ID 1
0823
App
ID 1
201
LUNA®
PHENYL-HEXYL(patented)
• Offers greater stability over common propyl-linked phenyl phases
• Selectivity benefits of both a phenyl and alkylphase combined
• Improved stability - pH 1.5 to 10.0 for over 2000 hours
Luna Phenyl-Hexyl employs a hexyl-linked phenyl phase. Thehexyl alkyl chain linker offers remarkable selectivity and greaterhydrolytic stability over the common propyl-linked chemistry.Luna Phenyl-Hexyl offers the chromatographer a high level ofperformance and durability along with excellent selectivity.
KEY:
Efficiency, Plates for Toluene
tR of Toluene (min)
k of Toluene
Symmetry of Toluene
Symmetry of Pyridine�
Antibacterials
Conditions for all columns:Dimensions: 150 x 4.6mmMobile Phase: A: 20mM KH2PO4, pH 2.5
B: AcetonitrileGradient: Start A/B (80:20) to A/B (75:25) in
5 min, go to A/B (55:45) in 15 minFlow Rate: 1.0 mL/minDetection: UV @ 254nm
Sample: 1. Carbadox2. Thiamphenicol3. Furazolidone4. Oxolinic Acid5. Sulfadimethoxine6. Sulfaquinoxaline7. Nalidixic Acid8. Piromidic Acid
5 10 15 20 min
12
3
45
67
8
LUNA® 5µPhenyl-Hexyl
Agilent TechnologiesZorbax® 5µSB-Phenyl
Waters Spherisorb® 5µPhenyl
Agilent TechnologiesZorbax® 5µ
Phenyl
Chromatographic Comparisons of Phenyl Columns*
*The comparative data presented here may not be representative for all applications.
pH Stability of Luna Phenyl-Hexyl
Luna
0 24 48 72 96 168 336 504 624 1008 1368 1632 1776 2256 24720
1
2
3
4
5
6
7
8
12000
10000
14000
Hours of Exposure
pH 1.5
0 24 48 72 96 168 336 504 624 1008 1368 1632 1776 2256 2472
Hours of Exposure
0
1
2
3
4
5
6
7
8
12000
10000
14000
pH 10.0
t R, k
, Sym
met
ry o
f To
luen
e an
d P
yrid
ine
Effi
cien
cy o
f Tol
uene
pl/c
olt R
, k, S
ymm
etry
of
Tolu
ene
and
Pyr
idin
eE
ffici
ency
of T
olue
ne p
l/col
Phenomenex148
HPLC
App
ID 1
4321
App
ID 1
4322
App
ID 1
4320
App
ID 3
464
LUNA®
AMINO (NH2)Luna Amino (NH2) was developed to improve column rugged-ness and reproducibility. What results is a unique amino, stablefrom pH 1.5 to 11.0, in 100% aqueous mobile phases, and inreversed and normal phases. For mono- and oligosaccharideanalysis or for any normal phase separation Luna Amino is anexcellent choice.
CYANO (CN)Luna CN shows remarkable performance and reproducibility.Luna’s revolutionary base silica has exceptional surface smooth-ness and consistency and minimizes variations during the bond-ing process. The result is a CN phase with excellent performanceand reproducibility batch-to-batch and column-to-column.
Column: Luna 5µ NH2 100ÅDimensions: 250 x 4.6mmOrder Number: 00G-4378-E0Mobile Phase: Acetonitrile/Water (80:20)Flow Rate: 3.0mL/minDetector: RITemperature: 40°CInjection Volume: 10µLSample: 1. Fructose (0.7mg/mL)
2. Glucose (0.75mg/mL)3. Sucrose (3mg/mL)4. Maltose (0.75mg/mL)
Column: LUNA® 5µ CyanoDimensions: 150 x 4.6mmMobile Phase: A=Hexane, B=Methylene chloride/
Methanol(80:20)A/B (80:20)
Flow Rate: 2.0 mL/minDetection: UV @ 254nmInjection volume: 1.0 µLTemperature: AmbientSample: 1. Hydrocortisone
2. Prednisone3. Cortisone4. Hydrocortisone Acetate
Testing Results for 3 Luna Amino Batches
Batch 2
Batch 3
Batch 1
Testing Results for 3 Luna Cyano Batches
Luna
Batch 1
Batch 2
Batch 3
Phenomenex 149
HPLC
App
ID 1
4548
App
ID 1
4549
App
ID 1
4550
App
ID 1
4547
12 min12 min1010882200
200200
100100
00
mAUmAU
6644
11
66
55
44
33
22
LUNA® SCX5µ 100Å
Macherey-NagelNucleosil® SA
5µ 100Å
Waters Spherisorb® SCX5µ 80Å
Whatman PartisilTM SCX5µ 85Å
10 min820
80
60
40
20
100
0
mAU
64
1
6
5
4
3
2
10 12 min820
80
60
40
20
0
mAU
64
1
4, 5
3, 6
2
10 12 min820
200
100
0
mAU
64
1, 2
6
5
4
3
LUNA®
Luna
STRONG CATION EXCHANGE (SCX)Luna SCX is a sil ica-based sorbent derivatized with benzene sulfonic acid phase to selectively separate amine-containing and cationic species from a mixture of neutral and anionic compounds. Conveniently packed with 5µ� foranalytical separation and 10µ� for scale-up, Luna SCX material has been shown to be reproducible and to have anoperating pH range between 2.0 to 7.0.
Luna SCX vs. Competition Luna C18 vs. SCX
App
ID 1
4554
App
ID 1
4553
LUNA® C18(2)5µ 100Å
4 6 min20
mAU
400
300
200
100
0
1
3
2
4
Cocaine and MetabolitesConditions for all columns:Dimensions: 150 x 4.6mmMobile Phase: 50mM KH2PO4, pH 2.5
/Acetonitrile (65:35)Flow Rate: 2.0 mL/minDetection: UV @ 210nmInjection Volume: 20µL
Sample: 1. m-Hydroxylbenzoyl Ecgonine2. Norbenzoyl Ecgonine3. Norcocaine4. Benzoyl Ecgonine Isopropyl Ester5. Cocaine6. Ecgonine Methyl Ester
Oxycodone Derviatives
Conditions for all columns:Dimensions: 150 x 4.6mmMobile Phase: 50mM KH2PO4,
pH 2.5/Acetonitrile (50:50)Flow Rate: 2.0 mL/minDetection: UV @ 210nmInjection Volume: 5µL
Sample: 1. Noroxymorphone2. Noroxycodone3. Oxymorphone4. Oxycodone
LUNA® SCX5µ 100Å
4 8 min620
mAU
100
80
60
40
20
120
140
0
1
23
4
Phenomenex150
HPLC
LUNA®
1
2
3
5
4
0 10 20 30 min
0
2
4
6
1
2
3
1 2 3 4 5 min
0
200
400
600
800
1
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0 2 4 6 8 min
0
40
80
120
160
2 4 6 8 10 12 14 16 min
1
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5
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1000
0 2 4 6 8 10 12 14
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2
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min
4 5
6
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8
Fatty Acids
Column: Luna 5µ C8(2)Dimensions: 150 x 4.6mmOrder No: 00F-4249-E0Mobile Phase: A: Acetonitrile
B: WaterGradient: A/B (70:30) to A/B (90:10) in 10 min,
to A/B (70:30) in 12 minFlow Rate: 0.3mL/minTemperature: 22°CDetection: Evaporative Light ScatteringSample: 1. Lauric Acid
2. Myristic Acid3. Palmitolic Acid4. Linoleic Acid5. Palmitic Acid6. Oleic Acid7. Heptadecanoic Acid8. Stearic Acid
Basic Drugs
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: 20mM KH2PO4, pH 7.0/Methanol (10:90)Flow Rate: 1.0 mL/minDetection: UV @ 254nmSample: 1. Pseudoephedrine
2. Diphenhydramine3. Amitriptyline
Steroids
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: 0.1% H3PO4 /Acetonitrile/Methanol (54:35:11)Flow Rate: 0.75 mL/minDetection: UV @ 254nmSample: 1. Hydrocortisone
2. Corticosterone3. 11-�-Hydroxyprogesterone4. Cortisone Acetate5. 11-Ketoprogesterone
Parabens
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: Acetonitrile/Water (50:50)Flow Rate: 1.0 mL/minDetection: UV @ 254nmSample: 25mg/mL of each:
1. Methylparaben2. Ethylparaben3. Propylparaben4. Butylparaben
Water-Soluble Vitamins
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: A: Water/Methanol/H3PO4 + 10mM sodium hexane sulfonate
(95 : 5 : 0.2)B: Methanol/Water/H3PO4 + 10mM sodium hexane sulfonate(50 : 50 : 0.2)
Gradient: 0% B to 100% B over 30 minFlow Rate: 1.5 mL/minDetection: UV @ 275nmSample: 1. Niacinamide
2. Pyridoxine3. Riboflavin4. Folic Acid5. Thiamine
App
ID 1
258
App
ID 1
185
App
ID 1
147
App
ID 1
208
App
ID 1
2913
Luna
Phenomenex 151
HPLC
LUNA®
54321 min
2
3
41
Cough and Cold Compounds
Column: Luna 5µ C8(2)Dimensions: 150 x 4.6mmOrder No.: 00F-4249-E0Mobile Phase: A: Methanol B: Acetonitrile w/0.1% H3P04
C: Water w/0.1% H3P04 and 0.1% heptane sulfonateA/B/C (40:10:50)
Flow Rate: 1.5mL/minTemperature: 22°CDetection: UV@ 214nmSample: 1. Acetaminophen
2. Pseudoephedrine3. Benzoic acid4. Chlorpheniramine5. Dextromethorphan
Narcotic Analgesics
Column: Luna 5µ C18Dimensions: 150 x 4.6mmOrder No.: 00F-4041-E0Mobile Phase: A: 20mM KH2PO4, pH 2.0
B: Acetonitrile/Methanol (80:20)A/B - (85:15)
Flow Rate: 1.0 mL/minDetection: UV @ 220nmSample: 1. Morphine
2. Codeine Phosphate3. Oxycodone4. Hydrocodone Bitartrate
Sugar Alcohols
Column: Luna 5µ NH2
Dimensions: 250 x 4.6mmOrder No : 00G-4378-E0Mobile Phase: Acetonitrile/Water (85:15)Flow Rate: 3 mL/minDetection: RITemperature: 40°CSample: 1. Glycerol
2. Pentaerythritol3. Xylitol4. Sorbitol5. Inositol
Explosives - EPA Method 8330
Column: Luna 5µ C18(2)Dimensions: 250 x 4.6mmOrder No.: 00G-4252-E0Mobile Phase: Methanol/Water (55:45)Flow Rate: 1.0 mL/minDetection: UV @ 254nmTemperature: 35°CSample: 1. HMX
2. RDX3. 1,3,5-Trinitrobenzene4. 1,3-Dinitrobenzene5. Tetryl6. Nitrobenzene7. 2,4,6-Trinitrotoluene8. 4-Amino-2,6-Dinitriotoluene9. 2-Amino-4,6-Dinitriotoluene
10. 2,6-Dinitrotoluene11. 2,4-Dinitrotoluene12. 2-Nitrotoluene13. 4-Nitrotoluene14. 3-Nitrotoluene
App
ID 1
4289
App
ID 1
164
App
ID 1
4263
App
ID 2
578
0 2 4 6 8 10 12 14 16
3
2
4
5
6
7
8
9
10
11
12
1314
1
Luna
Phenomenex152
HPLC
5 10 15 20 min
12
3
45
67
8
0 10 20 25
1
2
3
4
5
6
min
7
8
0 5 10 15 20
1
2
3
min
4
5
Saw Palmeto Berry, p-Bromophenacyl Esters
Column: Luna 3µ C8(2)Dimensions: 150 x 4.6mmOrder No.: 00F-4248-E0Mobile Phase: Acetonitrile/Water (87:13)Flow Rate: 1.5 mL/minTemperature: 25°CDetection: UV @ 254nmSample: 1. Capric Acid 5. Linoleic Acid
2. Lauric Acid 6. Palmitic Acid3. Linolenic Acid 7. Oleic Acid4. Myristic Acid 8. Stearic Acid
Antibacterials
Column: Luna 5µ Phenyl-HexylDimensions: 150 x 4.6mmOrder No.: 00F-4257-E0Mobile Phase: A: 20mM KH2PO4, pH 2.5
B: AcetonitrileGradient: A/B (80:20) to A/B (75:25) in 5 min,
to A/B (55:45) in 15 minFlow Rate: 1.0 mL/minDetection: UV @ 254nmSample: 1. Carbadox
2. Thiamphenicol3. Furazolidone4. Oxolinic Acid5. Sulfadimethoxine6. Sulfaquinoxaline7. Nalidixic Acid8. Piromidic Acid
Food Additives
Column: Luna 5µ Phenyl-HexylDimensions: 150 x 4.6mmOrder No.: 00F-4257-E0Mobile Phase: A: 50mM KH2PO4 + 0.1% H3PO4
B: AcetonitrileGradient: A/B (75:25) for 18 min,
to A/B (25:75) in 12 minFlow Rate: 1.0 mL/minDetection: UV @ 230nmSample: 1. Saccharin
2. p-Hydroxybenzoic Acid3. Sorbic Acid4. p-Hydroxybenzoic Acid Methyl Ester5. Dehydroacetic Acid6. p-Toluic Acid7. p-Hydroxybenzoic Acid Ethyl Ester8. n-Propyl p-Hydroxybenzoate
Antihistamines
Column: Luna 3µ C8 (2)Dimensions: 150 x 4.6mmOrder No.: 00F-4248-E0Mobile Phase: 0.5mM K2HPO4 /
Acetonitrile (52.6:47.4)Flow Rate: 1.3 mL/minTemperature: 25°CDetection: UV @ 249nmSample: 1. Promethazine Sulfoxide
2. N-Desmethylpromethazine3. Promethazine Hydrochloride4. Isopromethiazine5. Phenothiazine
App
ID 1
072
App
ID 1
107
App
ID 1
201
App
ID 1
199
LUNA®
Luna
Phenomenex 153
HPLC
0 0.5 1.0 1.5 2.0 2.5 min
0
5
10
15
20
25
mAU 12
0 2 4 6 8 10 min
1
2 3
4
5
512
3
4
0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min
0
25
50
75
100
125
150
mAU
0 10 20 min
1
4
2
3
2 4 6 8 10 12 min
3
1
2
0.2000
0.1000
0
Glucocorticoids
Column: Luna 5µ C8Dimensions: 150 x 4.6mmOrder No: 00F-4040-E0Mobile Phase: Acetonitrile/Water (50:50)Flow Rate: 1.0 mL/minDetection: UV @ 240nmSample: 1. Dexamethasone 21-Phosphate
2. Prednisolone3. Dexamethasone4. Prednisolone 21-Acetate5. Betamethasone 17-Valerate
Herbicides
Column: Luna 5µ C8(2)Dimensions: 150 x 4.6mmOrder No: 00F-4249-E0Mobile Phase: Acetonitrile/WaterGradient: 70 to 100% in 3 minFlow Rate: 1.0 mL/minDetection: UV @ 210nmSample: 1. Tebuthiuron
2. Simazine3. Altrazine4. Propizine5. Dacthol
Antibiotics
Column: Luna 5µ C8(2)Dimensions: 150 x 4.6mmOrder No: 00F-4249-E0Mobile Phase: Acetonitrile/Water (30:70)Flow Rate: 1.0 mL/minDetection: UV @ 365nmSample: 1. Oxytetracycline
2. Tetracycline
Acetaminophen, USP Method
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: Water/Methanol/Acetic Acid (69:28:3)Flow Rate: 1.5 mL/minDetection: UV @ 275nmSample: 1. Acetaminophen
2. Caffeine3. Benzoic Acid
Natural Products
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: A: Acetonitrile/Water (35:65)
B: Acetonitrile/Water (60:40)Gradient: 100% A in 1min to 100% B in 29 minFlow Rate: 1.5 mL/minDetection: UV @ 227nmSample: 1. Nordihydrocapsaicin
2. Capsaicin3. Dihydrocapsaicin4. Homocapsaicin
App
ID 1
284
App
ID 1
245
App
ID 1
260
App
ID 1
293
App
ID 1
076
LUNA®
Luna
Phenomenex154
HPLC
Polyamines (Dansylated)
Column: Luna 5µ C18 + Security GuardDimensions: 250 x 4.6mmOrder No: 00G-4252-E0 and KJ0-4282 and AJ0-4287Mobile Phase: A: 93% Methanol in Water
B: 65% Acetonitrile in WaterGradient: A/B (0:100) to A/B (100:0) in 28 min
to A/B (0:100) in 40 minutesTemperature: 35°CFlow Rate: 1.5 mL/minDetection: 360nm Excitation, 530nm EmissionSample: 1. Putrescine
2. DHX3. DHP4. Spermidine5. Spermine6. Homospermine7. Methylethylhomospermine8. Diethylhomospermine (DEHOP)
Water-Soluble Vitamins
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: A: Water/methanol, 2g/L hexane sulfonate,
0.2% H3PO4 (95:5)B: Water/methanol, 2g/L hexane sulfonate,0.2% H3PO4 (50:50)
Gradient: A/B (80:20) to A/B (27:73) in 17 minutesFlow Rate: 1 mL/minTempurature: 22°CDetection: UV-Vis Abs. - Variable Wave. @ 210-280 nmSample: 1. Calcium Pantothenate
2. Niacinamide3. Vitamin B64. Riboflavin5. Thiamine
Simple Sugars
Column: Luna 5µ NH2
Dimensions: 250 x 4.6mmOrder No: 00G-4378-E0Mobile Phase: Acetonitrile / Water (80:20)Temperature: 40°CFlow Rate: 3.0 mL/minDetection: RISample: 1. Xylose
2. Fructose3. Glucose4. Sucrose5. Maltose6. Melezitose7. Raffinose
PAH’s - EPA Method 8310
Column: Luna 5µ C18(2)Dimensions: 250 x 4.6mmOrder No: 00G-4252-E0Mobile Phase: A: Water
B: AcetonitrileGradient: A/B (25:75) to A/B (0:100) in 25 minTemperature: 22°CFlow Rate: 2.0 mL/minDetection: UV @ 254nmSample: 1. Naphthalene 9. Chrysene
2. Acenaphthalene 10. Benzo[e]pyrene3. Fluorene 11. Benzo[b]fluoranthene4. Phenanthrene 12. Benzo[k]fluoranthene5. Anthracene 13. Benzo[a]pyrene6. Fluoranthracene 14. Dibenzo[a,h]anthracene7. Pyrene 15. Benzo[g,h,i]perylene8. Benzo[a]anthracene 16. Indenol[1,2,3-c,d]pyrene
App
ID 1
288
App
ID 1
4285
App
ID 2
581
App
ID 1
4269
LUNA®
Luna
Phenomenex 155
HPLC
10 20 min0
1
2
3
4
5
0 0.5 1.0 1.5 2.0 2.5 3.0 min
0
10
20
30
40
50
mAU 1 23
1
2 3
4
5
0 5 10 15 20 min
Fatty Acid Amides
Column: Luna 5µ C18(2)Dimensions: 250 x 4.6mmOrder No: 00G-4252-E0Mobile Phase: A: 50mM Sodium phosphate pH 6.0
B: AcetonitrileGradient: A/B (75:25) to A/B (35:65) in 15 min,
then 5 min hold at A/B (35:65)Temperature: 50°CFlow Rate: 0.8 mL/minDetection: UV @ 214nmSample: 1. Acetamide
2. n-Butyramide3. Isovaleramide4. n-Hexanamide5. n-Heptamide6. n-Octanamide7. n-Decanamide
Caffeine and Metabolites
Column: Luna 5µ C18(2)Dimensions: 150 x 4.6mmOrder No: 00F-4252-E0Mobile Phase: 50mM Sodium Acetate/Acetonitrile/Methanol(92:4:4)Flow Rate: 1.0 mL/minDetection: UV @ 273nmSample: 1. Theobromine
2. Paraxanthine3. Theophylline4. Excipient5. Caffeine
Natural Products
Column: Luna 5µ Silica(2)Dimensions: 150 x 4.6mmOrder No: 00F-4274-E0Mobile Phase: Hexane/Dioxane (85:15)Flow Rate: 1.5 mL/minDetection: UV @ 230nmSample: 1. Dihydrokawain
2. Yangonin3. Kawain4. Dihydromethysticin5. Methysticin
Polar Herbicides
Column: Luna 3µ Phenyl-HexylDimensions: 100 x 4.6mmOrder No: 00D-4256-E0Mobile Phase: A: Acetonitrile B: WaterGradient: A/B (40:60) to A/B (40:60) in 3.0 min
to A/B (60:40) in 3.2 minutesTemperature: 22°CFlow Rate: 1 mL/minDetection: MS @ 195°CSample: 1. Simazine
2. Methabenzthiazuron3. Chlortoluron4. Atrazine5. Isoproturon6. Diuron7. Propazine8. Linuron9. Terbutryn
10. Trietazine
Benzodiazepines
Column: Luna 5µ Phenyl-HexylDimensions: 150 x 4.6mmOrder No: 00F-4257-E0Mobile Phase: Acetonitrile/Water (80:20)Flow Rate: 1.0 mL/minDetection: UV @ 235nmSample: 1. Lormetazepam
2. Diazepam3. Oxazepam
Tocopherols (Vitamin E)
Column: Luna 5µ NH2
Dimensions: 250 x 4.6mmOrder No: 00G-4378-E0Mobile Phase: Hexane/Isopropyl Alcohol (97:3)Flow Rate: 4.0 mL/minDetection: UV @ 298nmSample: 1. �-Tocopherol
2. �-Tocopherol3. �-Tocopherol4. �-Tocopherol
App
ID 1
291
App
ID 1
138
App
ID 1
255
App
ID 1
322
App
ID 1
4296
App
ID 1
243
LUNA®
Luna
Phenomenex156
HPLC
LUNA®
Luna
2 4 6 8 min
1 23
4
5
6
5 10 15 20 25 min
0
5000
10000
15000
20000
25000
30000
1 2
34
5
89
67
2 4 6 8 10 min12
1
2 34
5
Pesticides/Herbicides
Column: Luna 5µ CNDimensions: 150 x 4.6mmOrder No: 00F-4255-E0Mobile Phase: 18:82 Acetonitrile/Water to 65:35 in 5 minFlow Rate: 1.0 mL/minDetection: UV @ 254nmSample: 1. Tebuthiuron
2. Carbofuran3. Carbaryl4. Diuron5. Captan6. Barban
Carbamates
Column: Luna 3µ C18(2)Dimensions: 150 x 2.0mmOrder No: 00F-4251-B0Mobile Phase: A: Water B: AcetonitrileGradient: A/B (90:10) to A/B (30:70) in 20 min
to A/B (90:10) in 2 min then 5 min hold at A/B (90:10)Flow Rate: 0.2 mL/minDetection: API-ES (LC/MS)Temperature: 42°CSample: 1. Aldicarb Sulfoxide
2. Oxamyl3. Methomyl4. 3-Hydroxy Carbofuran5. Aldicarb6. Propoxur7. Carbofuran8. Carbaryl9. Methiocarb
Corticosteroids
Column: Luna 5µ CNDimensions: 150 x 4.6mmOrder No: 00F-4255-E0Mobile Phase: A: Hexane B: Methylene Chloride/Methanol (80:20)
80:20 A/BFlow Rate: 1.0 mL/minDetection: UV @ 254nmSample: 1. Desoxycorticosterone
2. Corticosterone3. Cortisone4. Prednisone5. Prednisolone
DNPH Aldehydes
Column: Luna 5µ C18(2)Dimensions: 250 x 4.6mmOrder No: 00G-4252-E0Mobile Phase: A: 99.9% Water, 0.1% THF
B: 99.9% Acetonitrile, 0.1% THFGradient: 70:30 (A/B) linear to 30:70 over 30 minutesFlow Rate: 1.5mL/minDetection: UV @ 380nmTemperature: AmbientInjection Volume: 25µLSample: 1. Formaldehyde DNPH (.14µg)
2. Acetaldehyde DNPH3. Acetone DNPH4. Acrolein DNPH5. Propionaldehyde DNPH6. Butyraldehyde DNPH7. Benzaldehyde DNPH
App
ID 1
2924
App
ID 1
2923
App
ID 2
546
App
ID 1
292
DeoxynucleosidesColumn: Luna 5µ SCXDimensions: 150 x 4.6mmOrder No: 00F-4398-E0Mobile Phase: 50mM KH2PO4, pH 2.5Flow Rate: 1.5 mL/minDetection: UV @ 210nmInjection Volume: 5µLSample: 1. d-Uridine 4. d-Cytidine
2. d-Inosine 5. d-Adenosine3. d-Guanosine
App
ID 1
4557
1412108642 18 min0 16
1
5
43
2
Phenomenex 157
HPLC
LUNA®
Luna
Luna Bonded Phase Selectivity ChartDescription Application
C5 5, 10µA reversed-phase chemistry that offers greater hydrolytic stability and Highly hydrophobic compounds. Good alternative to C8 or C18 whenalternative selectivity over the classic C4 phase. less retention is desired.
C8(2) 3, 5, 10, 10-PREP, 15µC8 phase for excellent efficiency, peak shape and resolution. Significantly Great starting point for methods development of pharmaceuticals,improved performance over traditional C8 phases due to high surface nucleotides and polar compounds. Excellent for highly aqueous andcoverage. Slightly lower carbon load than original Luna C8. LC/MS applications.
C8 5, 10µOriginal Luna C8 phase. Highly retentive C8 optimized for long lifetimes at A “workhorse” for extremely high pH applications, or when a moreextremely high pH levels. retentive C8 is desired.
C18(2) 3, 5, 10, 10-PREP,15µC18 phase. Excellent efficiency, peak shape and resolution. Slightly lower A C18 phase for virtually all HPLC applications. Range of particle sizescarbon load than original Luna C18. offers amazing versatility for capillary and LC/MS, to prep and
process scale applications. Our No.1 recommended choice for HPLCmethod development.
C18 5, 10µOriginal Luna C18 phase. Very high retention, optimized for long lifetimes at A “workhorse” for extremely high pH applications, or when longerextremely high pH levels. retention is desired.
Phenyl-Hexyl 3, 5, 10, 10-PREP,15µA (patented) phenyl phase which employs a hexyl alkyl linker as opposed to Remarkable selectivity for aromatic compounds. Offers selectivitythe traditional propyl chain. Offers great stability as well as alternative advantages of both a phenyl phase and a C6 or C8 alkyl phase. Stable,selectivity. reproducible alternative to traditional phenyl phases. Also very good
for amine and polar compound selectivity.CN 3, 5, 10µ
Cyano phase. Can be used as reversed- or normal-phase material. The use of Excellent for improving the retention of polar compounds. ExtremelyLuna base silica results in overall phase reproducibility and performance. rapid equilibration makes it very good for rapid screening and gradient
applications. Exceptional normal phase performance.NH
23, 5, 10µAmino Phase. Can be used in reversed or normal phase modes. Stable from Excellent for sugar analysis or anionic compounds by reversed phasepH 1.5 to 11.0 and under 100% aqueous conditions. High performance silica chromatography and for compounds capable of hydrogen bondingand bonding techniques produces a rugged, highly reproducible column. under normal phase conditions.
SCX 5, 10µA Benzene Sulfonic Acid bonded phase is used to make this Strong Cation Excellent for cationic and nitrogen containing analytes such as coughExchange (SCX) column. Offers great peak shape and resolution. and cold compounds, codeine and morphine alkaloids, and drugs of
abuse. Also useful for 2-dimension chromatography (SCX-RP-MS-MS)of small peptides and protein digests.
for ID: 2.0-3.0mm 3.2-8.0mmSee p. 263 for Fused Silica Capillary Adapterand Capillary Guard Columns.
3µ and 5µ Capillary Columns (mm)
50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 50 x 0.70 150 x 0.70 250 x 0.70Phases3µ C8(2) I 00B-4248-AC I 00F-4248-AC I 00G-4248-AC I 00B-4248-AF I 00F-4248-AF I 00G-4248-AF I 00B-4248-AG I 00F-4248-AG I 00G-4248-AG3µ C18(2) 00B-4251-AC 00F-4251-AC 00G-4251-AC 00B-4251-AF 00F-4251-AF 00G-4251-AF 00B-4251-AG 00F-4251-AG 00G-4251-AG5µ C8(2) 00B-4249-AC 00F-4249-AC 00G-4249-AC 00B-4249-AF 00F-4249-AF 00G-4249-AF 00B-4249-AG 00F-4249-AG 00G-4249-AG5µ C18(2) 00B-4252-AC 00F-4252-AC 00G-4252-AC 00B-4252-AF 00F-4252-AF 00G-4252-AF 00B-4252-AG 00F-4252-AG 00G-4252-AG
ORDERING INFORMATION
3µ Microbore and Minibore Columns (mm) SecurityGuard™Cartridges
30 x 1.0 50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0mm 4 x 3.0mmPhasesSilica(2) I — I — I — I 00A-4162-B0 I 00B-4162-B0 I 00D-4162-B0 I 00F-4162-B0 AJO-4347 I AJO-4348C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJO-4289 AJO-4290C18(2) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJO-4286 AJO-4287CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304 AJ0-4305Phenyl-Hexyl 00A-4256-A0 00B-4256-A0 — 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJO-4350 AJO-4351NH
2— — 00F-4377-A0 00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJO-4301 AJO-4302
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
for ID: 2.0-3.0mm 3.2-8.0mm
ORDERING INFORMATION
3µ Analytical Columns (mm) SecurityGuard™Cartridges
30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0mm 4 x 3.0mmPhasesSilica(2) I — I — I 00F-4162-Y0 I — I 00B-4162-E0 I — I 00D-4162-E0 I 00F-4162-E0 AJ0-4347 I AJ0-4348C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351NH
2— 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 00C-4377-E0 00D-4377-E0 00F-4377-E0 AJO-4301 AJO-4302
If Luna does not provide at least an equivalent separation as compared to acompeting column of the same particle size, similar phase and dimensions, sendin your comparative data within 45 days and keep the Luna column for FREE.
Phenomenex158
HPLC
LUNA®
Luna
ORDERING INFORMATION
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
for ID: 2.0-3.0mm 3.2-8.0mm
MercuryMS™ LC/MS Cartridges*
10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0Qty Pricing
3µ Phase I I I I
LUNA C18(2) 00N-4251-B0-CE 00N-4251-D0-CE 00M-4251-B0-CE 00M-4251-D0-CELUNA C8(2) 00N-4248-B0-CE 00N-4248-D0-CE 00M-4248-B0-CE 00M-4248-D0-CE5µLUNA C18(2) 00N-4252-B0-CE 00N-4252-D0-CE 00M-4252-B0-CE 00M-4252-D0-CELUNA C8(2) 00N-4249-B0-CE 00N-4249-D0-CE 00M-4249-B0-CE 00M-4249-D0-CE
for ID: 2.0-3.0mm 3.2-8.0mm
MercuryMS Cartridges
require holder, see pg. 266
5µ Microbore and Minibore Columns (mm) SecurityGuard™Cartridges
50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0mm 4 x 3.0mmPhasesSilica(2) I — I — I — I — I 00B-4274-B0 I 00F-4274-B0 I 00G-4274-B0 AJ0-4347 I AJ0-4348C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292 AJ0-4293C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 00G-4040-B0 AJ0-4289 AJ0-4290C8 (2) 00B-4249-A0 00F-4249-A0 — 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289 AJ0-4290C18 — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286 AJ0-4287C18 (2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286 AJ0-4287CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304 AJ0-4305Phenyl-Hexyl 00B-4257-A0 00F-4257-A0 — 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350 AJ0-4351NH2 — 00F-4378-A0 00G-4378-A0 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301 AJ0-4302
5µ Analytical Columns (mm) SecurityGuard™Cartridges
30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0mm 4 x 3.0mmPhasesSilica(2) I — I — I — I — I — I 00B-4274-E0 I — AJ0-4347 I AJ0-4348C5 — — 00F-4043-Y0 — — 00B-4043-E0 — AJ0-4292 AJ0-4293C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290C18 — — 00F-4041-Y0 00G-4041-Y0 00A-4041-E0 00B-4041-E0 00C-4041-E0 AJ0-4286 AJ0-4287C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351NH
2— 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302
SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308
Phenomenex 159
HPLCfor ID: 2.0-3.0mm 3.2-8.0mm 9-16mm
ORDERING INFORMATION SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
LUNA®
See p. 207 for Semi-Prep and Prep
SecurityGuard Cartridge Holders
Luna
5µ Analytical, Semi-Prep and Preparative Columns (mm) SecurityGuard™ Cartridges
100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 4 x 2.0mm 4 x 3.0mm 10 x 10mmPhasesSilica(2) I 00D-4274-E0 I 00F-4274-E0 I 00G-4274-E0 I 00G-4274-N0 I — I 00G-4274-P0 I 00G-4274-U0 AJ0-4347 I AJ0-4348 I AJ0-7223C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 00G-4043-N0 — — — AJ0-4292 AJ0-4293 AJO-7372C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — AJ0-4289 AJ0-4290 AJ0-7222C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0 00G-4249-UO AJ0-4289 AJ0-4290 AJ0-7222C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — AJ0-4286 AJ0-4287 AJ0-7221C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0 00G-4252-U0 AJ0-4286 AJ0-4287 AJ0-7221CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — — — AJ0-4304 AJ0-4305 AJ0-7313Phenyl-Hexyl 00D-4296-E0 00F-4396-E0 00G-4396-E0 00G-4396-N0 00G-4396-AK 00G-4396-P0 00G-4396-U0 AJ0-4350 AJ0-4351 AJ0-7314NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — 00G-4378-P0 00G-4378-U0 AJ0-4301 AJ0-4302 AJ0-7364SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 — 00G-4398-P0 — AJ0-4307 AJ0-4308 AJ0-7369
15µ Preparative and Pilot Scale Columns (mm) Guards
250 x 4.6 250 x 15 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 50PhasesSilica(2) I 00G-4271-E0 I 00G-4271-AK I 00G-4271-P0 I 00G-4271-U0 I 00G-4271-V0 I 00G-4271-W0 03B-4271-V0C8(2) 00G-4272-E0 00G-4272-AK 00G-4272-P0 00G-4272-U0 00G-4272-V0 00G-4272-W0 03B-4272-V0C18(2) 00G-4273-E0 00G-4273-AK 00G-4273-P0 00G-4273-U0 00G-4273-V0 00G-4273-W0 03B-4273-V0Phenyl-Hexyl 00G-4286-E0 00G-4286-AK 00G-4286-P0 00G-4286-U0 00G-4286-V0 00G-4286-W0 03B-4286-V0
for ID: 9-16mm
10µ Semi-Prep and Preparative Columns (mm) Guards Cartridges
250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 50 10 x 10mmPhasesSilica(2) I 00G-4091-E0 I 00G-4091-N0 I 00G-4091-AK I 00G-4091-P0 I 00G-4091-U0 I 00G-4091-V0 I 00G-4091-W0 03B-4091-V0 AJO-7223C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0 — — — 03B-4092-V0 AJO-7372C8 00G-4093-E0 00G-4093-N0 — 00G-4093-P0 — 00G-4093-V0 00G-4093-W0 03B-4093-V0 AJO-7222C8(2) 00G-4250-E0 00G-4250-N0 00G-4250-AK 00G-4250-P0 00G-4250-U0 00G-4250-V0 00G-4250-W0 03B-4250-V0 AJO-7222C18 00G-4094-E0 00G-4094-N0 — 00G-4094-P0 — 00G-4094-V0 00G-4094-W0 03B-4094-V0 AJO-7221C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0 00G-4253-U0 00G-4253-V0 00G-4253-W0 03B-4253-V0 AJO-7221CN 00G-4300-E0 00G-4300-N0 — 00G-4300-P0 00G-4300-U0 00G-4300-V0 00G-4300-W0 03B-4300-V0 AJO-7313Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 00G-4285-AK 00G-4285-P0 00G-4285-U0 00G-4285-V0 00G-4285-W0 03B-4285-V0 AJ0-7314NH
200G-4379-E0 00G-4379-N0 — 00G-4379-P0 00G-4379-U0 00G-4379-V0 00G-4379-W0 03B-4379-V0 AJ0-7364
SCX 00G-4401-E0 00G-4401-N0 — 00G-4401-P0 — — — 03B-4401-V0 AJ0-7369
SecurityGuard™
Phenomenex160
HPLC
5µ Columns (mm)
30 x 2.0 50 x 2.0 30 x 4.6 50 x 4.6PhasesSilica(2) I — I 00B-4274-B0 I — I 00B-4274-E0C5 00A-4043-B0 00B-4043-B0 — 00B-4043-E0C8 00A-4040-B0 00B-4040-B0 00A-4040-E0 00B-4040-E0C8 (2) 00A-4249-B0 00B-4249-B0 00A-4249-E0 00B-4249-E0C18 00A-4041-B0 00B-4041-B0 00A-4041-E0 00B-4041-E0C18 (2) 00A-4252-B0 00B-4252-B0 00A-4252-E0 00B-4252-E0
LUNA®
Luna
METHODDEVELOPMENTLuna Method Development Kits combine the excellent perfor-mance of Luna HPLC columns with either 3 or 5 different se-lectivities. The column kits include a Luna Phenyl-Hexyl (mod-erate polarity) and your choice of a Luna CN (polar) or a C8(2)or C18(2) (non-polars).
METHODVALIDATION KITSThree columns of the same phase from three different batchesprovide an excellent benchmark for method validation. Methodreproducibility is easily and quickly assessed, clearly demon-strating chromatographic performance validation potential andlong term compliance with Luna Method Validation Kits.
COMBI-HTS™ HIGH-THROUGHPUTSCREENING COLUMNSNew, 21.2mm and 30mm ID columns provide exceptional loadability and durabilityfor high-throughput purifications. Luna Combi-HTS columns utilize the same Lunamaterial, but undergo additional QC at higher flow rates to ensure excellent perfor-mance under high pressure and high flow conditions.
See p.268 for LC/MS Computer-Based Training Program.
ORDERING INFORMATION
See p.264 for MercuryMS LC/MS Cartridge Column System.
ORDERING INFORMATION
3µ Columns (mm)
30 x 2.0 50 x 2.0 30 x 4.6 50 x 4.6PhasesSilica(2) I 00A-4162-B0 I 00B-4162-B0 I — I 00B-4162-E0C8(2) 00A-4248-B0 00B-4248-B0 00A-4248-E0 00B-4248-E0C18(2) 00A-4251-B0 00B-4251-B0 00A-4251-E0 00B-4251-E0
Method Development Kits
Order No. Description Dimensions (mm) Unit Price
KH0-4760I
Luna Method Development Kit (5 columns)I I I
1 each of 5µ C18(2), C8(2), C5, CN, Phenyl-Hexyl phases 150 x 4.6 5/pkKH0-4354 Luna Rapid-MD Kit (3 columns)
1 each of 3µ C18(2), C8(2) and Phenyl-Hexyl phases 50 x 4.6 3/pkKHO-4761 Luna Selectivity Kit 1 (3 columns)
1 each of 5µ C18(2), Phenyl-Hexyl, CN phases 150 x 4.6 3/pkKH0-4762 Luna Selectivity Kit 2 (3 columns)
1 each of 5µ C8(2), Phenyl-Hexyl, CN phases 150 x 4.6 3/pk
Method Validation Kits
Order No. Description Dimensions (mm)* Unit Price
KH0-4355I
Luna 5µ C18 Method Validation KitI
150 x 4.6I
3/pkI
KH0-4356 Luna 5µ C18(2) Method Validation Kit 150 x 4.6 3/pkKH0-4357 Luna 5µ C8 Method Validation Kit 150 x 4.6 3/pkKH0-4358 Luna 5µ C8(2) Method Validation Kit 150 x 4.6 3/pkKH0-4359 Luna 5µ Phenyl-Hexyl Method Validation Kit 150 x 4.6 3/pk*Other dimensions available on request.
5µ Luna Combi-HTS Columns (mm)
50 x 21.2 100 x 21.2 50 x 30 75 x 30 100 x 30PhasesCombi-HTS C8(2) I 00B-4384-P0 I 00D-4384-P0 I 00B-4384-U0 I 00C-4384-U0 l 00D-4384-U0Combi-HTS C18(2) 00B-4383-P0 00D-4383-P0 00B-4383-U0 00C-4383-U0 00D-4383-U0
Phenomenex 161
HPLC
LUNA®
Luna
BULK MATERIALORDERING INFORMATION
10µ Bulk Packings
100g 1kg 5kg 10kg 50kg 100kgPhasesSilica(2) I 04G-4091 I 04K-4091 I 04L-4091 I 04M-4091 I 04N-4091 I 04P-4091Bonded SilicaC5 04G-4092 04K-4092 04L-4092 04M-4092 04N-4092 04P-4092C8 04G-4093 04K-4093 04L-4093 04M-4093 04N-4093 04P-4093C8(2) 04G-4250 04K-4250 04L-4250 04M-4250 04N-4250 04P-4250C18 04G-4094 04K-4094 04L-4094 04M-4094 04N-4094 04P-4094C18(2) 04G-4253 04K-4253 04L-4253 04M-4253 04N-4253 04P-4253CN 04G-4300 04K-4300 04L-4300 04M-4300 04N-4300 04P-4300NH
204G-4379 04K-4379 04L-4379 04M-4379 04N-4379 04P-4379
SCX 04G-4401 04K-4401 04L-4401 04M-4401 04N-4401 04P-4401Phenyl-HexylPhenyl-Hexyl 04G-4285 04K-4285 04L-4285 04M-4285 04N-4285 04P-4285
15µ Bulk Packings
100g 1kg 5kg 10kg 50kg 100kgPhasesSilica(2) I 04G-4271 I 04K-4271 I 04L-4271 I 04M-4271 I 04N-4271 I 04P-4271Bonded SilicaC8(2) 04G-4272 04K-4272 04L-4272 04M-4272 04N-4272 04P-4272C18(2) 04G-4273 04K-4273 04L-4273 04M-4273 04N-4273 04P-4273Phenyl-HexylPhenyl-Hexyl 04G-4286 04K-4286 04L-4286 04M-4286 04N-4286 04P-4286
10µ-PREP Bulk Packings
Lower cost excellent performance alternative to LUNA 10µ.
100g 1kg 5kg 10kg 50kg 100kgPhasesSilica(2) I 04G-4322 I 04K-4322 I 04L-4322 I 04M-4322 I 04N-4322 I 04P-4322Bonded SilicaC8(2) 04G-4323 04K-4323 04L-4323 04M-4323 04N-4323 04P-4323C18(2) 04G-4324 04K-4324 04L-4324 04M-4324 04N-4324 04P-4324Phenyl-HexylPhenyl-Hexyl 04G-4325 04K-4325 04L-4325 04M-4325 04N-4325 04P-4325