Luminex Multiplex

Assays

Applications include:

Signal transduction path way evaluation

Sample Sources: Tissue culture conditioned media, whole blood / isolated PBMC, serum/plasma, biological fluids

Species: human, mouse, rat, canine, non-human primate

Analytes: Signaling proteins (phospho and total), Cytokines, Chemokines

Data Generation: Concentration (cytokine, chemokine and signal ing molecules), IC

50

determination

ROCK

Rho

FAK CrK

Pax PYK2

GTP Gα-O

GEFs

CDC42 Rac

MEKs

PAK MKKs

ASK1

JNK

P38 IP3R

PKC

BIMP1

BCL10

NFκB IκBs

P

IKKs

IκBsDegradation

Src

SOSGRB2SHC

RasGEFs

Raf1

MEKs

ERKs

PLC-β

DAG

NFκB

IKKs

PκB

IP3

Ca+2Ca+2

Ca+2

Calm

P

Caln

NFAT

NFAT

P

PI3K

Gβ Gγ

LARG

BRaf

MEKs

ERKs

cAMP

EPACPKA

PDZRhoGEF

Gα-1 GTP

Rap1

p115RhoGEF

RT

KS

CREB

Gα12/13

SRF

GTP

CREB

SRF ELK-1

c-Jun

c-Fos

STAT

NFκBNFAT

Gene Expression

Gα Gβ

© 2013 CDT all rights reserved

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EFTECHNICAL BRIEF:

Luminex Multiplex AssaysD I S C O V E R Y T E C H N O L O G I E S

As the information around signaling pathways and poten-

tially drugable targets expands, there is increasing pressure

to quickly probe these pathways. There is a greater need to

understand the nature of protein-protein interactions within

signaling pathways and to identify biomarkers of metabolic

processes. Singleplex detection methods such as ELISA or

Western blot assays are not suitable for rapid investigation

of complex signaling pathways composed of several mem-bers. Luminex technology offers the ability to multiplex

detection of several proteins from a single biological sample.

The open architecture of Luminex technology allows for

multiplexing of many types of assays thus reducing time,

labor, and costs over traditional methods.

Applications The Confluence Discovery Technologies team has a wide range of experience in developing and validating Luminex Multiplex assays to suit many different requirements. Our highly trained and knowledgeable scientists will assist with experimental design to develop and validate assays to meet client-specified criteria. We also perform data analysis and interpretation of Luminex Multiplex data to enable our clients to obtain the best insights from large, complex data outputs.

ROCK

Rho

FAK CrK

Pax PYK2

GTP Gα-O

GEFs

CDC42 Rac

MEKs

PAK MKKs

ASK1

JNK

P38 IP3R

PKC

BIMP1

BCL10

NFκB IκBs

P

IKKs

IκBsDegradation

Src

SOSGRB2SHC

RasGEFs

Raf1

MEKs

ERKs

PLC-β

DAG

NFκB

IKKs

PκB

IP3

Ca+2Ca+2

Ca+2

Calm

P

Caln

NFAT

NFAT

P

PI3K

Gβ Gγ

LARG

BRaf

MEKs

ERKs

cAMP

EPACPKA

PDZRhoGEF

Gα-1 GTP

Rap1

p115RhoGEF

RT

KS

CREB

Gα12/13

SRF

GTP

CREB

SRF ELK-1

c-Jun

c-Fos

STAT

NFκBNFAT

Gene Expression

Gα Gβ

© 2013 CDT all rights reserved

CDTv1.8.13

Confluence Discovery Technologies, Inc.4320 Forest Park Avenue

Saint Louis, MO 63108Phone: 314-932-4032 x305

Fax: 314-932-4038www.confluencediscovery.com

CDTinfo@confluencestl.com

D I S C O V E R Y T E C H N O L O G I E S

CAPABILITIESD I S C O V E R Y T E C H N O L O G I E S

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Leaders in biochemistry and mechanistic enzymology, our senior team has access to a suite of capabilities and technologies enabling: • Drug:Target interaction • Enzyme:Inhibitor mechanisms • Direct binding kinetics • Compound mechanism of action

Con�uence o�ers a spectrum of cell and molecular biology capabilities: • Identi�cation of biologically important signal transduction pathways • Quantitation of e�cacy and selectivity • Understanding safety issues linked to pathways • De-convolution to isolate pathway nodes • Evaluation of multiple targets within the pathway • Identi�cation of key pathway point(s) for therapeutic intervention • Target validation, cloning, expression, and puri�cation • Custom assay development • High-throughput screening

Our team delivers when “OFF the SHELF” is not an option: • Assay development, optimization, and validation • Multiplex technology platforms and instrumentation • Complex data analysis • Specialty screens for preclinical technical application • Trouble shooting and problem solving

Our team provides clients with the expertise to: • Identify target, mechanism, and disease biomarkers • Select the appropriate biomarkers for a drug discovery program • Execute biomarker assay design, development, validation, and interpretation for pre-clinical and clinical studies • Seamless assay transfer from pre-clinical to clinical studies

Our medicinal and computational chemists are experts in designing and delivering novel chemical entities through: • Schrödinger® enabled computational drug design • High throughput hit prioritization • Chemical series prioritization • Structure activity relationship generation • Custom synthesis