lesson 2: transport and translocation of water and solutes

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Bachelor 2: Plant physiology Lesson 2 : TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES Instructor: LE Thi Van Anh [email protected] 1

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Page 1: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Bachelor 2: Plant physiology

Lesson 2:

TRANSPORT AND TRANSLOCATION

OF WATER AND SOLUTES

Instructor: LE Thi Van Anh

[email protected] 1

Page 2: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

By the end of this course, students are able to:

-  Describe some basic terms: transportation, translocation and

transpiration

-  Understand 2 processes: passive and active cellular transport

- Describe the different routes of water and solute mineral absorption

from soil to root

-  Analyze the different forces ensuring the transport in xylem

-  Describe the process of transpiration through stomata

Learning  outcome  

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Page 3: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Concept:  

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What kind of transport in plants:

………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………  

What must be transported in plants?…………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………  

Page 4: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Focusing…  

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1)  Cellular transport 2)  Water & Mineral Absorption of Roots 3)  Transport of Xylem Sap 4)  Control of Transpiration 5)  Translocation of Phloem Sap

Page 5: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Cellular transport…  

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Passive Active ………………………………………………………. …………………………………………… ………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

Page 6: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Cellular transport: passive water transport…  

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Moves from high H2O potential to a low H2O potential

Flaccid cell dropped into 0.1M sucrose solution

Water come into cell

Turgid cell

Water go out

Add more sucrose

Page 7: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Cellular transport: passive solute transport…  

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Solutes move from greater to lower osmolarity

Page 8: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Permeability of cellular membrane  

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Why???

Page 9: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Cellular transport: transport protein  

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Three classes of membrane transport protein

Page 10: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Hypothetical model for cation transport by a pump: Energy comes from ATP hydrolysis « primary active transport »

Cellular transport: carrier protein  

Page 11: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Two types of secondary transport

Cellular transport: pump protein  

Page 12: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

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Overview of the various transport processes on the plasma membrane and tonoplast of plant cells

Page 13: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Water Absorption of Roots

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Page 14: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Water Absorption of Roots

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………………………………………………………. …………………………………………… ………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

Page 15: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Root pressure

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- Force (pressure) caused by water bulk flow from soil to xylem. -  Manometer can measure positive pressure about 0.05 to 0.2 MPa

Guttation: exudation of xylem sap through hydrathodes

Page 16: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Ion Absorption of Roots and xylem loading

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1. Cortex: …………………………………………………………………………………………………………………………………………

2. From endodermis to xylem parenchyma: …………………………………………………………………………………………………………………………………………

3. Enter to tracheary elements …………………………………………………………………………………………………………………………………………

Page 17: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Transport in xylem  

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How does water move up to xylem? ………………………………………………………. ……………………………………………

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Page 18: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Cohesion-­‐tension  theory  

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Page 19: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Transpira6on:  water  movement  to  atmosphere    

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Types  of  transpira6on    

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How many types of transpiration?

Page 21: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Transpira6on  through  stomata  

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Page 22: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Water  pathway  through  the  leaf  

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Page 23: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Transpira6on  ra6o  

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Transpiration ratio (TR) = water transpired/carbon dioxide assimilated For C3 plant, TR= 1/400

TR is used to evaluate the “water use efficiency”

How to increase “water use efficiency”?

Page 24: Lesson 2: TRANSPORT AND TRANSLOCATION OF WATER AND SOLUTES

Adapta6on  for  using  water  efficiently  

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