lecture 8 tuesday, january 22, 2008logan/teaching/human_biochem/lect 8 jan 22.pdf · lecture 8 ....

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Lecture 8 Tuesday, January 22, 2008 1. Central Dogma DNA, RNA structures. Replication, transcription, and translation 2. Exploring Genes and Genomes Fragmenting, sequencing, amplifying genes Recombinant technology Eukaryotic genomes PCR Animation Vector / Plasmid maps Fluorescent nucleotides. Chapter Problems: Chapter 3: 1, 4, 10, 11, 14, 15, 17, 18. Chapter 4: 1, 3 (assume B-form helix), 5, 7, 10 (why would U be used in RNA but T in DNA, given this chemistry?), 11, 12, 13, 20, 22, 23 (why might the protein sequence be more conserved than the DNA sequence?) Chapter 5: 1, 2, 5, 7, 8, 12, 13, 14, 15.

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Page 1: Lecture 8 Tuesday, January 22, 2008logan/teaching/human_biochem/Lect 8 Jan 22.pdf · Lecture 8 . Tuesday, January 22, 2008. 1. Central Dogma • DNA, RNA structures. • Replication,

Lecture 8 Tuesday, January 22, 2008

1. Central Dogma • DNA, RNA structures. • Replication, transcription, and translation

2. Exploring Genes and Genomes • Fragmenting, sequencing, amplifying genes • Recombinant technology • Eukaryotic genomes

• PCR Animation • Vector / Plasmid maps • Fluorescent nucleotides.

Chapter Problems: Chapter 3: 1, 4, 10, 11, 14, 15, 17, 18. Chapter 4: 1, 3 (assume B-form helix), 5, 7, 10 (why would U be used in RNA but T in

DNA, given this chemistry?), 11, 12, 13, 20, 22, 23 (why might the protein sequence be more conserved than the DNA sequence?)

Chapter 5: 1, 2, 5, 7, 8, 12, 13, 14, 15.

Page 2: Lecture 8 Tuesday, January 22, 2008logan/teaching/human_biochem/Lect 8 Jan 22.pdf · Lecture 8 . Tuesday, January 22, 2008. 1. Central Dogma • DNA, RNA structures. • Replication,

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Page 8: Lecture 8 Tuesday, January 22, 2008logan/teaching/human_biochem/Lect 8 Jan 22.pdf · Lecture 8 . Tuesday, January 22, 2008. 1. Central Dogma • DNA, RNA structures. • Replication,

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