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Laboratory water purification systems

2

Choosing a water purification system

Application ISO 3696 classification

Suggested model and configuration Page

Feed water for labware washers

Grade 3

Sterifeed 20

Feed water for autoclaves Crystal 7 RO 18

Glassware rinsing Crystal E RO 15

Feed water for automated biochemistry analyzers Grade 2 Crystal Clinic 22

General laboratory work

Grade 2

Crystal 7 Pure 18Electrochemistry

Flame AASCrystal E Pure 15

Wet chemistry

SpectrophotometryCrystal B Pure 10

Buffer and media preparation

Reagent preparation

Grade 1

Crystal E Trace 12Ion chromatography

ICP-MS

Crystal B Trace 3Graphite AAS

ICP-OES

HPLC

Grade 1

Crystal E HPLC 12Crystal B HPLC 3

Gas chromatography Integrity HPLC 6TOC measurements Onsite HPLC 8Flow cytometry

Grade 1

Crystal E Bio 12Crystal B Bio 3

Cell and tissue cultureIntegrity Bio 6

Molecular biology Onsite Bio 8

3

UltrapUre water systems

Crystal B ultrapure systems are multi-purpose water purification systems. The Crystal B systems produce ultrapure and pure water directly from tap water.

Crystal B Ultrapure systems are available in the following configurations:

Crystal B Trace• (P/N CB-1015) produces water for inorganic trace analysis. Water can be used for atomic absorption spectrometry (with graphite furnace atomizer), ICP-OES ana-lysis, ICP-MS and other inorganic analytical methods.Crystal B HPLC• (P/N CB-1101) produces water with very low or-ganic carbon (TOC) content mee-ting requirements of liquid chro-matography methods. Crystal B HPLC water can also be used for some microbiological and molecular biology appli cations.Crystal B Bio• (P/N CB-1201) system produces water with very low organic and RNase/DNase content that is intended for the use in molecular biology, including RNase-sensitive appli-cations.

Any configuration of a Crystal B ultrapure system produces both ultrapure and pure water. Ultrapure (Grade 1) water is dispensed through the point-of-use filter on the front panel. Pure (Grade 2) water is dispensed directly from the storage tank. Crystal B ultrapure water can be used for the most demanding appli cations including, but not li-mited to: • inorganic trace analysis • liquid chromatography • cell culture • molecular biology

With resistivity of 18.2 Mega-Ohm*cm (0.055 μS/cm) ultrapure water produced by a Crystal B system exceeds requirements of all relevant standards (ISO 3696 Grade 1, ASTM Type I, CLSI Type I). Purified water is collected in a storage tank. An integrated recirculation system ensures consistent quality of water and reduces total organic carbon (TOC) to very low levels: <2ppb for “HPLC” and “Bio” configurations and 5-10 ppb for the “Trace” configuration.

Pure water produced by the Crystal B systems complies with the requirements of ISO 3696 Grade 2 water and can be used for labware washing, wet chemistry methods, flame spectrophotometers, etc. The dispense rate of ultrapure water is 2 L/min. Whereas the dispense rate of pure water is 4 L/min.

All Crystal B systems have a controller with a graphic LCD display for water quality indi-cation. The LCD display provides all necessary information about sys-

tem status, as well as the remaining pre-filter life and deionization (DI) module performance. The smart DI module monitoring system also provides a reduction in running costs. A user is instructed to replace the DI module only when the module is near the end of its service life.

If a Bluetooth option (P/N 10103) is installed, the controller can be de-tached, so the Crystal B system can be controlled remotely. If space-saving is crucial, wall-mounting the Crystal B, and the use of a re-mote control is the right choice. All cartridges and filters are easily ac-cessible and no tools are required to replace them. The Crystal B sys-tem can be installed on a labora-tory bench or mounted on a wall.

The Crystal B has important safety functions:

tank filling control •tap water pressure control •protection from tank sensor failure •

The Crystal B systems include:Boost pump•Pre-filter set•Reverse osmosis module•Deionization module•Final stage polishing module•25L storage tank with an inte-•grated Grade II dispensing pumpRecirculation system•Point-of-use final filter (0.22 μm •microfilter for the Crystal B Trace and HPLC systems)Ultrafilter for the Crystal B Bio •systems; and/or a photo-oxida-tion module for elimination of organics (HPLC and Bio configu-rations)UV sterilization module (Bio •configuration)

Crystal B series

4

Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input

StrainerReverseosmosismodule

(RO module)

CheckvalveFlowrestrictor

Pure waterqualitysensor

Pure waterdispense

Storagetank

Pure (Grade 2)water dispense pump

Deionizationmodule

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter

Ultrapurewater qualitysensor

Ultrapure waterdispense valve

Ultrapure waterdispense

Final pointof-use filter

Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)


Dual wavelengthUV photooxidationmodule (185/254 nm)


Pure waterdispense

Storagetank


Deionizationmodule

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter





UV sterilization module

(254 nm)Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)


Dual wavelength UV photooxidation module (185/254 nm)


Pure waterdispense

Storagetank


Deionizationmodule

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter





Crystal B Trace

Crystal B HPLC

Crystal B Bio

Flow diagrams

SpecificationsPurified water specifications Crystal B

TraceCrystal B

HPLCCrystal B

Bio

Pure (Grade 2) water resistivity >10 MΩ x cm >10 MΩ x cm >10 MΩ x cm

Pure (Grade 2) water conductivity <0.1 μS/cm <0.1 μS/cm <0.1 μS/cm

Ultrapure (Grade 1) water resistivity 18.2 MΩ x cm 18.2 MΩ x cm 18.2 MΩ x cm

Ultrapure (Grade 1) water conductivity 0.055 μS/cm 0.055 μS/cm 0.055 μS/cm

TOC 5 – 10 ppb <2 ppb <2 ppb

RNase – – <0.01 ng/mL

DNase – – <4 pg/μL

Bacteria < 1 cfu/mL < 1 cfu/mL < 1 cfu/mL

Endotoxins <0.15 EU/mL <0.15 EU/mL < 0.001 EU/mL

Particles > 0.22 μm <1/ per mL <1/ per mL <1/ per mL

Nominal flow, pure water (to storage tank) 10 L/h 10 L/h 10 L/h

Nominal dispense flow, ultrapure water 2 L/min 2 L/min 2 L/min

Nominal dispense flow, pure water 4 L/min 4 L/min 4 L/min

Deionization module life (standard module) 1 m3 1 m3 1 m3

Recovery >30 % >30 % >30 %

Dimensions (WxDxH), cm 50x40x60 50x40x60 50x40x60

Storage tank 25 L 25 L 25 L

Tank dimensions (WxDxH), cm 30x25x50 30x25x50 30x25x50

Feed water pressure 0.5 – 5 bar 0.5 – 5 bar 0.5 – 5 bar

Feed water conductivity < 900 μS/cm < 900 μS/cm < 900 μS/cm

Applications

Application Crystal B Trace

Crystal BHPLC

Crystal BBio

Reagent preparation • • •Ion chromatography • • •ICP-MS • • •Atomic absorption spectrophotometry with graphite atomizer

• • •ICP-OES • • •HPLC – • •Gas chromatography – • •Total Organic Carbon measurement – • •Flow cytometry – – •Cell and tissue culture – – •Molecular biology – – •

5

ConsumablesPart

number Description Replacement criteria Comments

10019 Replacement pre-filter set, Crystal B Filter life counter is zero or the filter is clogged

10310 Replacement deionization module „DI Err” message is shown, or water conductivity is consistently > 0.5 μS/cm

10029 Replacement polishing module Every 1–2 years, depending on operation

10011 Replacement sterilization UV bulb As required (on average – every 3 years) „Bio” systems only

10018 Replacement photooxidation UV bulb As required (on average – every 3 years) „HPLC” and „Bio” systems

10012 Replacement 0.22 μm dispense microfilter Every 6–12 months „Trace” and „HPLC” systems

10120 Replacement ultrafilter Every 6–12 months „Bio” systems only

ComponentsPart

number Accessory Crystal B Trace

Crystal B HPLC

Crystal B Bio

10019 Pre-filter set for chlorine and organics reduction + + +

Boost pump + + +

Reverse osmosis module + + +

10310 Deionization module, standard + + +

10029 Polishing module + + +

10105 Photo-oxidation module – + +

10012 Point-of-use microfilter + + –

10109 Point-of-use ultrafiltration module – – +

10102 UV sterilization module – – +

10106 Integrated TOC monitor – option –

10103 Removable controller with Bluetooth module option option option

Model Part number

Crystal B Trace CB-1015

Crystal B HPLC CB-1101

Crystal B Bio CB-1201

Ordering Information

6


Crystal Integrity systems produce ultrapure water directly from tap water. Ultrapure water produced by the Crystal Integrity system complies with the requirements for Grade 1 water in accordance with relevant standards (ISO 3696, ASTM, CLSI). The Crystal Integrity systems are recommended for laboratories with relatively low consumption of ultrapure water (about 5 litres per day).Crystal Integrity systems are available in the following confi-gurations:

Integrity HPLC System• (P/N CB-1503) produces water with very low organic carbon (TOC) content. This meets the re qui-rements for liquid chromatog-raphy methods. Integrity HPLC water can also be used for some microbiological and molecular biology appli cations.

Integrity Bio System• (P/N CB-1505) produces water with very low organic and RNase/DNase content. This is intended for use in molecular biology, including RNase-sensitive applications.

Crystal Integrity water is intended for the most demanding applica-tions including, but not limited to: • inorganic trace analysis • liquid chromatography • cell culture • molecular biology Ultrapure water produced by an Integrity system, with resistivity of 18.2 MegaOhm*cm (0.055 μS/cm), exceeds requirements of all rel-evant standards (ISO 3696 Grade 1, ASTM Type I, CLSI Type I). Purified water is collected in an in-tegrated storage tank. The recircu-lation system ensures consistent quality of water and reduces total organic carbon (TOC) to very low levels: <2ppb.Space-saving, integrated, 5L tank is made of high density polyeth-ylene to minimize contamination. The storage tank is accessible for cleaning.

All Integrity systems have a con-troller with a graphic LCD display for water quality indication. The LCD display provides all necessary information about system status, remaining pre-filter life, and pol-ishing module performance. The smart polishing module monitor-ing system provides a reduction in running costs. Users are instructed to replace the polishing module only when the module is close to the end of its service life.

If a Bluetooth option (P/N 10103) is installed, the controller can be detached. The Integrity system can be controlled remotely for more convenient operation. All cartridges and filters are eas-ily accessible and no tools are re-quired to replace them. The Integ-rity system can be installed on a laboratory bench or mounted on the wall to save space.

The Crystal Integrity has impor-tant safety functions, including:

tank filling control•tap water pressure control•

Integrity series

ConsumablesPart


10019 Replacement pre-filter set, Integrity Filter life counter is zero or the filter is clogged

10029 Replacement polishing module„DI Err” message is shown, or water conductivity while dispensing is consistently > 0.1 μS/cm

10011 Replacement sterilization UV bulb As required (on average – every 3 years) „Bio” systems only

10018 Replacement 0.22 μm dispense microfilter As required (on average – every 3 years) „HPLC” and „Bio” systems

10012 Replacement 0.22 μm dispense microfilter Every 6–12 months „HPLC” systems only10120 Replacement ultrafilter Every 6–12 months „Bio” systems only

Applications

Application IntegrityHPLC

IntegrityBio

Reagent preparation • •Ion chromatography • •ICP-MS • •Atomic absorption spectrophotometry with graphite atomizer • •ICP-OES • •HPLC • •Gas chromatography • •Total Organic Carbon measurement • •Flow cytometry – •Cell and tissue culture – •Molecular biology – •

ComponentsPart

number Accessory Integrity HPLC

IntegrityBio

10019 Pre-filter set for chlorine and organics reduction reduction + +

Reverse osmosis module + +

10029 Polishing module + +

10105 Photo-oxidation module + +

10012 Point-of-use microfilter + –

10109 Point-of-use ultrafiltration module – +

10102 UV sterilization module – +

10106 Integrated TOC monitor option –

10103 Removable controller with Bluetooth module option option


(254 nm)

Inputvalve

Activatedcarbon/5um

pre-filterDrain

Pressureswitch

Input


(RO module)




Storagetank

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter





Model Part number

Crystal Integrity HPLC

CB-1503

Crystal Integrity Bio CB-1505

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SpecificationsPurified water specifications Integrity HPLC Integrity Bio

Ultrapure (Grade 1) water resistivity 18.2 MΩ x cm 18.2 MΩ x cm

Ultrapure (Grade 1) water conductivity 0.055 μS/cm 0.055 μS/cm

TOC <2 ppb <2 ppb

RNase – <0.01 ng/mL

DNase – <4 pg/μL

Bacteria < 1 cfu/mL < 1 cfu/mL

Endotoxins <0.15 EU/mL < 0.001 EU/mL

Particles > 0.22 μm <1/ per mL <1/ per mL

Nominal flow, pure water (to storage tank) 4 L/h 4 L/h

Nominal dispense flow, ultrapure water 2 L/min 2 L/min

Polishing module life 1 m3 1 m3

Recovery >30 % >30 %

Dimensions (WxDxH), cm 50x40x60 50x40x60

Integrated storage tank 5 L 5 L

Feed water pressure 1.5 – 5 bar 1.5 – 5 bar

Feed water conductivity < 900 μS/cm < 900 μS/cm

Inputvalve

Activatedcarbon/5um

pre-filterDrain

Pressureswitch

Input


(RO module)




Storagetank

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter





Integrity HPLC

Integrity Bio

Flow diagrams


88


Adrona Onsite systems produce ultrapure water from pre-treated water. Ultrapure water produced by the Adrona Onsite complies with the requirements for Grade 1 water for all relevant standards (ISO 3696, ASTM, CLSI). Adrona Onsite systems are recom-mended for laboratories with consumption of ultrapure water of 5-10 litres per day.Adrona Onsite systems are avail-able in following configurations:

Onsite HPLC System• (P/N CB-1803) produces water with very low organic carbon (TOC) con-tent. This meets the require-ments of liquid chromatography methods. Onsite HPLC water can also be used for some microbio-logy and molecular biology ap-plications.Onsite Bio System• (P/N CB-1805) produces water with very low organic and RNase/DNase content. This is intended for use in molecular biology, including RNase-sensitive applications.

Water produced by Adrona Onsite systems is intended for most de-

manding applications including, but not limited to: • inorganic trace analysis • liquid chromatography • cell culture • molecular biology Ultrapure water produced by an Integrity system, with resistivity of 18.2 MegaOhm*cm (0.055 μS/cm), exceeds requirements of all rele-vant standards (ISO 3696 Grade 1, ASTM Type I, CLSI Type I). Crystal Onsite systems have integrated sto -rage tank. The storage tank should be filled with pre-treated water by the operator. Pre-treated water may be obtained from a water still, or a reverse osmosis system. The Crystal Onsite system comes with a 5L carboy. The carboy has a stopcock and handle for conve-nient transportation of water from a water still to the Crystal Onsite unit. The recirculation system ensures a consistent quality of water and re-duces total organic carbon (TOC) to a very low level: <2ppb.A space-saving, integrated, 5L tank is made of high-density polyethy-

lene to minimize contamina-tion. The 5L tank is accessible for cleaning.All Crystal Onsite systems have a controller with a graphic LCD dis-play for water quality indication. The LCD display provides all the necessary information about sys-tem status, remaining pre-filter life, and the polishing module performance. The smart polishing module monitoring system pro-vides a reduction in running costs. The user is instructed to replace the polishing module only when the module is close to the end of it’s service life.If a Bluetooth option (P/N 10103) is installed, the controller can be detached. The Onsite system can be controlled remotely for conve-nient operation. The Crystal Onsite has important safety functions including tank filling control. All cartridges and filters are easily accessible and no tools are required to replace them. The Onsite system can be installed on a laboratory bench or mounted on the wall to save space.

Onsite series

Applications

Application OnsiteHPLC

OnsiteBio

Reagent preparation • •Ion chromatography • •ICP-MS • •Atomic absorption spectro-photometry with graphite atomizer • •ICP-OES • •HPLC • •Gas chromatography • •Total Organic Carbon measurement • •Flow cytometry – •Cell and tissue culture – •Molecular biology – •

ComponentsPart

number Accessory Onsite HPLC

OnsiteBio

10029 Polishing module + +

10105 Photooxidation module + +

10012 Point-of-use microfilter + –

10109 Point-of-use ultra filtration module – +

10106 Integrated TOC monitor option –

10103 Removable controller with Bluetooth module option option

99

SpecificationsPurified water specifications Onsite

HPLCOnsite

Bio

Ultrapure (Grade 1) water resistivity 18.2 MΩ x cm 18.2 MΩ x cm

Ultrapure (Grade 1) water conductivity 0.055 μS/cm 0.055 μS/cm

TOC <2 ppb <2 ppb

RNase – <0.01 ng/mL

DNase – <4 pg/μL

Bacteria < 1 cfu/mL < 1 cfu/mL

Endotoxins <0.15 EU/mL < 0.001 EU/mL

Particles > 0.22 μm <1/ per mL <1/ per mL

Nominal dispense flow, ultrapure water 2 L/min 2 L/min

Polishing module life 1 m3 1 m3



Integrated storage tank 5 L 5 L


ConsumablesPart


10029 Replacement polishing module„DI Err” message is shown, or water conductivity while dispensing is consistently > 0.1 μS/cm

10018 Replacement 0.22 μm dispense microfilter As required (on average – every 3 years) „HPLC” and „Bio” systems

10012 Replacement 0.22 μm dispense microfilter Every 6–12 months „HPLC” systems only”

10120 Replacement ultrafilter Every 6–12 months „Bio” systems only


Storagetank

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter






Storagetank

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter





Onsite Bio

Onsite HPLC

Flow diagrams

Model Part number

Crystal Onsite HPLC CB-1803

Crystal Onsite Bio CB-1805


The Crystal B Pure systems pro-duce purified water for general laboratory applications and ana-lytical inorganic methods. Crystal B Pure water complies with the requirements of ISO 3696 Grade 2. The costs of Pure water produc-tion are lower in comparison to the costs of Ultrapure water. Pure (Grade 2) water complies with the requirements of a variety of appli-cations, including:

Many inorganic methods of •analysis (e. g. flame spectrom-etry)Wet chemistry methods•Electrochemistry•Labware washing, etc.•

The Crystal B Pure systems are designed to provide a lab with water that is essentially free from dissolved ions and has a low TOC content. The conductivity of Crys-tal B Pure water is less than 0.1 μS/cm. This exceeds the requirements for Grade 2 water put forth in ISO 3696.

Crystal B Pure systems are equipped with a user-friendly control module. The control mod-ule has a graphic LCD display with indication of water quality. The Bluetooth option (P/N 10103) pro-vides remote control of the Crystal B system via the detachable con-troller. The Crystal B system can be installed on a wall or under a bench, thus saving valuable labo-ratory space.

The controller module provides information about the system sta-tus; including remaining pre-filter life and deionization (DI) module performance. The smart DI mo-

10

Crystal B Pure series

Applications

Application Crystal B Pure

Glassware rinsing •Laboratory washers •Autoclaves •Electrochemistry •Flame AAS •Wet chemistry •Spectrophotometry •Buffer and media preparation •

Specifications

Purified water specifications Crystal B Pure

Purified water resistivity >10 MΩ x cm

Purified water conductivity <0.1 μS/cm

Particles > 0.22 μm <1 per mL

Nominal flow, pure water (to storage tank) 10 L/h

Dispense flow, pure water 4 L/min

Deionization module life (standard module) 1 m3

Recovery >30 %

Dimensions (WxDxH), cm 50x40x60

Storage tank (option) 25 L – 300 L

Feed water pressure 0.5 – 5 bar

Feed water conductivity < 900 μS/cm

pUre water systems

11

Flow diagram

Crystal B Pure

Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)



Pure waterdispense

Storagetank


Deionizationmodule

Model Part number

Crystal B Pure CB-1005

ConsumablesPart


10019Replacement pre-filter set, Crystal B

Filter life counter is zero or the filter is clogged

10310Replacement deionization module

„DI Err” message is shown, or water conductivity is consistently > 0.5 μS/cm

10011Replacement sterilization UV bulb

As required (on average every 3 years)

Systems with 10102 option only

ComponentsPart

number Accessory Crystal B Pure

10019 Pre-filter for chlorine and organics reduction +

Boost pump +

Reverse osmosis module +

10310 Deionization module, standard +

10102 UV sterilization module option

10103 Removable controller with Bluetooth module option

dule monitoring system provides a reduction in running costs. The user is instructed to replace the DI module only when the module is near the end of it’s service life.

A storage tank for purified water is available as an option. We of-fer storage tanks with capacities ranging from 10L – 60L. All tanks with a capacity of 25L and higher have integrated pumps for fast and convenient dispensing. The dispensing flow rate is 4 L/min. An integrated pump enables place-ment of the storage tank under a bench, if desired, to save more space. The Crystal B has important safety functions, including: Tank filling control, tap water pres-sure control, and protection from tank sensor failure.

The Crystal B systems include: Boost pump•Pre-filter set •Reverse osmosis module •Deionization module•

A UV sterilization module is avail-able as an option (P/N 10102).

Storage tanksPart

number Accessory Crystal B Pure

11000 Storage tank “Basic” w/dispense pump, 25L option

11014 Storage tank “Tiny”, 10L option

10007 Storage tank “Comfort” w/dispense pump, 60L option

10004 Storage tank “Economy” w/dispense pump, 50L option


Model Part number

Crystal E Trace CE-1001

Crystal E HPLC CE-1101

Crystal E Bio CE-1201


12

Crystal E series


The Crystal E ultrapure systems are economy class, multi-purpose, water purification systems.

Crystal E ultrapure water systems are available in the following configurations:

Crystal E Trace System• (P/N CE-1001) produces water for inor-ganic trace analysis. This water is recommended for atomic absorption spectrometry (with graphite furnace atomizer), ICP-OES analysis, ICP-MS and other inorganic analytical methods.Crystal E HPLC System• (P/N CE-1101) produces water with very low organic carbon content (TOC) to comply with the re-quirements of liquid chromatog-raphy methods. Crystal E HPLC water can also be used for some microbiological and molecular biology applications.Crystal E Bio System• (P/N CE-1201) produces water with very low organic and RNase/DNase content, intended for molecular biology, including RNase-sensi-tive applications.

All Crystal E systems produce two types of water: Ultrapure (ISO 3696 Grade 1) and Pure (ISO 3696 Grade 2). Ultrapure water produced by the Crystal E systems has resistiv-ity 18.2 MegaOhm*cm (conduc-tivity 0.055 μS/cm). This exceeds requirements of all the relevant standards (ISO 3696 Grade 1, ASTM Type I, CLSI Type I). Puri-fied water is collected in a storage tank. The recirculation system en-sures a consistent quality of water, and a low level of organic carbon content (TOC). TOC is <2ppb for “HPLC” and “Bio” configurations; and 5-10 ppb for the “Trace” con-figuration. The dispensing rate of high-quali-ty, Ultrapure water is 2 L/min.

Pure water produced by Crystal E systems can be used for labware washing, wet chemistry methods, flame spectrophotometers, etc.. Pure water is dispensed directly from the storage tank. The dis-pensing flow rate of Pure water is 4 L/min.All systems have a graphic LCD display that provides a clear water-quality readout. The display also provides information about the system status. This includes cur-rent resistivity and remaining pre-filter service life. The smart deion-ization (DI) module performance monitoring system provides a reduction in running costs. All car-tridge and filter replacement can

be done by the operator and no tools are required. The Crystal E systems include important safety functions, such as:

tank filling control•tap water pressure control•protection from a tank sensor •failure

Running costs can be decreased even more by installing an 8L deionization module option (P/N 10101).

Crystal E systems include:Boost pump•Pre-filter•RO module•Deionization module•Polishing module•25L storage tank with integrated •Grade 2 dispensing pumpRecirculation system•Point-of-use Final Filter:•

0.22 μm microfilter for Crystal •E Trace and HPLC systemsultrafilter for Crystal E Bio •systems

Photo-oxidation module for •reduction of organics (for HPLC and Bio configurations)UV sterilization module (for the •Bio configuration)

13

Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)



Pure waterdispense

Storagetank


Deionizationmodule

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter





Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)



Pure waterdispense

Storagetank


Deionizationmodule

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter






Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)



Pure waterdispense

Storagetank


Deionizationmodule

Recirculationpump

Shuntingrestrictor

Recirculationvalve

Polishingmodule

Protectivefilter







(254 nm)

Crystal E Trace

Crystal E HPLC

Crystal E Bio

Flow diagrams

ConsumablesPart


10009 Replacement pre-filter, Crystal E Filter life counter is zero or the filter is clogged

10310 Replacement deionization module


10113 Replacement high-capacity deionization module

„DI Err” message is shown, or water conductivity is consistently > 0.5 μS/cm Systems with 10101 option only

10029 Replacement polishing module Every 1–2 years, depending on operation

10011 Replacement sterilization UV bulb As required (on average every 3 years) ”Bio” systems only

10018 Replacement photo-oxidation UV bulb As required (on average every 3 years) ”HPLC” and “Bio” systems only

10012 Replacement 0.22 μm dispense microfilter Every 6–12 months “Trace” and “HPLC” systems

10120 Replacement ultrafilter Every 6–12 months “Bio” systems only

ComponentsPart Accessory Crystal E

TraceCrystal E

HPLCCrystal E

Bio

10009 Pre-filter for chlorine and organics reduction + + +

Boost pump + + +Reverse osmosis module + + +

10310 Deionization module, standard + + +

10101

High capacity deionization module, (replaces standard module)

option option option

10029 Polishing module + + +10105 Photo-oxidation module – + +10012 Point-of-use microfilter + + –

10109 Point-of-use ultrafiltration module – – +

10102 UV sterilization module – – +

14

SpecificationsPurified water specifications Crystal E

TraceCrystal E

HPLCCrystal E

BioGrade 2 water resistivity >10 MΩ x cm >10 MΩ x cm >10 MΩ x cmGrade 2 water conductivity <0.1 μS/cm <0.1 μS/cm <0.1 μS/cm

Grade 1 water resistivity 18.2 MΩ x cm 18.2 MΩ x cm 18.2 MΩ x cm

Grade 1 water conductivity 0.055 μS/cm 0.055 μS/cm 0.055 μS/cm

TOC 5 – 10 ppb <2 ppb <2 ppb

RNase N/A N/A <0.01 ng/mL

DNase N/A N/A <4 pg/μL

Bacteria < 1 cfu/mL < 1 cfu/mL < 1 cfu/mL

Endotoxins <0.15 EU/mL <0.15 EU/mL < 0.001 EU/mL

Particles > 0.22 μm <1 per mL <1 per mL <1 per mL


Nominal dispense flow, ultrapure water 2 L/min 2 L/min 2 L/min

Nominal dispense flow, pure water 4 L/min 4 L/min 4 L/min

Deionization module life (standard module) 1 m3 1 m3 1 m3

Deionization module life (high capacity module) 3 m3 3 m3 3 m3

Recovery >30 % >30 % >30 %


Storage tank 25 L 25 L 25 L

Tank dimensions (WxDxH), cm 30x25x50 30x25x50 30x25x50

Feed water pressure 0.5 – 5 bar 0.5 – 5 bar 0.5 – 5 barFeed water conductivity < 900 μS/cm < 900 μS/cm < 900 μS/cm

ApplicationsApplication Crystal E

TraceCrystal E

HPLCCrystal E

Bio

Reagent preparation • • •Ion chromatography • • •ICP-MS • • •Atomic absorption • • •ICP-OES • • •HPLC – • •Gas chromatography – • •Total Organic Carbon – • •Flow cytometry – – •Cell and tissue culture – – •Molecular biology – – •

15

Crystal E RO and Pure systems pro-duce purified water of ISO 3696 Grade 3 (RO) and ISO 3696 Grade 2 (Pure). Pure and RO water have much lower production costs than ultrapure (Grade 1) water. Pure and RO water comply with the require-ments of a variety of applications, including:

Many inorganic methods of •analysis (e. g. flame spectropho-tometry)Wet chemistry methods•Electrochemistry•Labware washing, etc.•

The Crystal E RO system reduces contamination in water by more than 98%. The conductivity of Crystal E RO purified water is typi-cally less than 5 μS/cm (dependent

upon feed water).The Crystal E Pure system produces water that is essentially free from inorganic contamination, and low in TOC content. The conductivity of water produced by the Crystal E Pure system is less than 0.1 μS/cm. This exceeds the requirements for Grade 2 water according to ISO 3696.Storage tanks of various sizes are available as an option for the Pure water system. The capacity of the tanks range from 10L to 300L. All tanks with a capacity of 25L or higher have integrated pumps for fast and convenient dispensing. The dispensing flow rate is 4 L/min. An integrated pump allows place-ment of the storage tank under a bench, if needed, to save space.The Crystal E RO and Crystal E Pure have a graphic LCD display pro-viding indication of water qual-ity, system status, and remaining pre-filter life. The smart DI module performance monitoring system provides a reduction in system running costs. All cartridge and

filter replacement can be done by the operator without tools. The Crystal E provides important safe-ty functions, including:

tank filling control•tap water pressure control•protection from tank sensor •failure.

High-Consumption Laboratories and Applications:We offer the Crystal E20 Pure sys-tem for high Pure water consump-tion (30 L/day and more) applica-tions. This includes a high capacity (8L) deionization module. The high capacity module provides a reduc-tion in system running costs. It is also possible to order 8L module option (P/N 10101) for the Crystal E Pure system.The Crystal E systems include:

Boost pump•Pre-filter•Reverse osmosis module•Deionization module (for Pure •systems only)

A UV-sterilization module is avail-able as an option (P/N 10102).

Crystal E Pure and RO series

SpecificationsPurified water specifications Crystal E RO Crystal E20 Pure Crystal E Pure

Purified water resistivity >10 MΩ x cm >10 MΩ x cm

Purified water conductivity <5 μS/cm <0.1 μS/cm <0.1 μS/cm

Particles > 0.22 μm <1 per mL <1 per mL <1 per mL


Dispense flow, pure water 4 L/min 4 L/min 4 L/min

Deionization module life (standard module) N/A N/A 1 m3

Deionization module life (high capacity module) N/A 3 m3 3 m3 (optional)

Recovery >30 % >30 % >30 %


Storage tank (option) 25 L – 300 L 25 L – 300 L 25 L – 300 L

25L tank dimensions (WxDxH), cm 30x25x50 30x25x50 30x25x50

Feed water pressure 0.5 – 5 bar 0.5 – 5 bar 0.5 – 5 bar

Feed water conductivity < 900 μS/cm < 900 μS/cm < 900 μS/cm

pUre water systems

16

Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)



Pure waterdispense

Storagetank


Deionizationmodule

Crystal E Pure

Inputvalve

Activatedcarbon/5um

pre-filter

Boostpump

Drain

Pressureswitch

Input


(RO module)



Pure waterdispense

Storagetank


Crystal E RO

Drain

Reverseosmosismodule

(RO module)

Checkvalve

Flowrestrictor


Pure waterdispense

Storagetank


Deionizationmodule

Inputvalve

Inputvalve

Activatedcarbon/5um

pre-filter

Activatedcarbon/5um

pre-filter Boostpump

Pressureswitch

Pressureswitch

Input

Strainer

Strainer

Crystal E20 Pure

Flow diagrams

17

Applications

Application Crystal E RO

Crystal E Pure

Glassware rinsing • •Laboratory washers • •Autoclaves • •Electrochemistry – •Flame AAS – •Wet chemistry – •Spectrophotometry – •Buffer and media preparation – •

Model Part number

Crystal E Pure CE-1005

Crystal E RO CE-1245

Crystal E20 Pure C20-1001HC

Consumables

Part number Description Replacement criteria Comments

10009 Replacement pre-filter, Crystal E RO, Pure


10039 Replacement pre-filter set, Crystal E20 Pure


10310 Replacement deionization module

„DI Err” message is shown, or water conductivity is consistently > 0.5 μS/cm “Pure” systems only

10113 Replacement high-capacity deionization module


Only “E20 Pure” or systems with 10101 option

10011 Replacement sterilization UV bulb As required (on average every 3 years) Only systems with 10102 option

10012 Replacement 0.22 μm dispense filter Every 6–12 months Only systems with 10201 option

ComponentsPart

number Accessory Crystal E RO

Crystal E20 Pure

Crystal E Pure

10009 Pre-filter for chlorine and organics reduction + - +

10039 Pre-filter set for chlorine and organics reduction - + -

Boost pump + + +Reverse osmosis module + + +

10310 Deionization module, standard – – +

10101 High capacity deionization module, (replaces standard module) – + option

10201 Point-of-use microfiltration module for Pure systems – option option

10102 UV sterilization module – option option

Storage tanks

Part number Accessory Crystal E

ROCrystal

E20 PureCrystal E

Pure

11000 Storage tank “Basic” w/dispense pump, 25L option option option

10007 Storage tank “Comfort” w/dispense pump, 60L option option option

10004 Storage tank “Economy” w/dispense pump, 50L option option option

11014 Storage tank “Tiny”, 10L option option option





18

The Crystal 7 Pure system (P/N C7-1001) is an entry level water purifi-cation system with very low system running costs. It is wall-mounted and does not require any space on a laboratory bench. The Crystal 7 Pure system produces purified water for general laboratory ap-plications and analytical methods. Crystal 7 system water is used for many applications, including:

Many inorganic methods of •analysis (e. g. flame spectro-photometry)Wet chemistry methods•Electrochemistry•Labware washing, etc.•

The Crystal 7 Pure system is a re-liable source of purified lab water with conductivity < 0.1 μS/cm (re-sistivity > 10 MegaOhm*cm). This exceeds requirements for Grade 2 water according to ISO 3696. The operating costs of a Crystal 7 Pure system are extremely low.

The Crystal 7 RO system (P/N C7-1241) produces water that com-plies with ISO 3696 Grade 3 re-quirements.

The Crystal 7 Pure and RO systems are equipped with a graphic LCD display providing system status info and water quality monitoring readouts. The remaining pre-filter life and deionization (DI) module performance are shown on the LCD display. The smart DI module monitoring system provides a re-duction in system operating costs. Users are instructed to replace the DI module only when the module is near the end of its service life.Storage tanks for purified water are an available option. We offer storage tanks ranging in capac-ity from 10L – 60L. Tanks of 25L or more have integrated pumps for fast and convenient dispens-ing. The dispensing flow rate is 4 L/min. All cartridges and filters are easy to access and replace. An inte-grated pump allows users to place the storage tank under a bench, if desired, to save space.The Crystal 7 system has impor-tant safety functions, including:

tank filling control•tap water pressure control•protection from tank sensor •failure

The Crystal 7 systems include:boost pump•pre-filter set•reverse osmosis module•deionization module•

A UV-sterilization module is avail-able as an option (P/N 10102).

Crystal 7 RO and Pure series

SpecificationsPurified water specifications Crystal 7 RO Crystal 7 Pure

Purified water resistivity >10 MΩ x cm

Purified water conductivity <5 μS/cm <0.1 μS/cm

Particles > 0.22 μm <1 per mL <1 per mLNominal flow, pure water (to storage tank) 7 L/h 7 L/h

Dispense flow, pure water 4 L/min 4 L/min

Deionization module life N/A 1 m3



Storage tank (option) 10 L – 60 L 10 L – 60 L

Feed water pressure 0.5 – 5 bar 0.5 – 5 bar


Storage tanksPart

number Accessory Crystal 7 RO

Crystal 7 Pure

11000 Storage tank “Basic” w/dispense pump, 25L option option

10007 Storage tank “Comfort” w/dispense pump, 60L option option

10004 Storage tank “Economy” w/dispense pump, 50L option option

11014 Storage tank “Tiny”, 10L option option

Model Part number

Crystal 7 Pure C7-1001

Crystal 7 RO C7-1241


pUre water systems

19

Activatedcarbon/5um

pre-filter

1μmsedimentfilter

Inputvalve

Boostpump

Drain

Pressureswitch

Input


(RO module)

Checkvalve

Flowrestrictor


Pure waterdispense

Storagetank


Activatedcarbon/5um

pre-filter

1μmsedimentfilter

Inputvalve

Boostpump

Drain

Pressureswitch

Input


(RO module)

Checkvalve

Flowrestrictor


Pure waterdispense

Storagetank


Deionizationmodule

Applications

Application Crystal 7 RO

Crystal 7 Pure

Glassware rinsing • •Laboratory washers • •Autoclaves • •Electrochemistry – •Flame AAS – •Wet chemistry – •Spectrophotometry – •Buffer and media preparation – •

Consumables


10014Replacement pre-filter set, Crystal 7


10015Replacement deionization module



As required (on average every 3 years)Systems with 10102 option only

ComponentsPart

number Accessory Crystal 7 RO

Crystal 7 Pure

10014 Pre-filter set for chlorine and organics reduction + +

Boost pump + +

Reverse osmosis module + +

10015 Deionization module pack – +

10102 UV sterilization module – option

Crystal 7 RO

Crystal 7 Pure

Flow diagrams

20

Sterifeed is a water purification system that is designed to produ-ce feed water for autoclaves.

The Sterifeed system produces water with conductivity of 5-11 uS/cm. This is within the recom-mended conductivity range of 3-15 uS/cm, presented by autocla-ve manufacturers.

Water with a conductivity reading above 15 uS/cm facilitates salt deposits on heating elements, eventually leading to the failure of the heating element. Water with a conductivity below 3 uS/cm can attack internal surfaces of the au-toclave, thusly damaging water le-vel sensors, heating elements, and connections.

Sterifeed water provides optimal conductivity, within the range of 5-11 uS/cm, granting reliable ope-ration of the autoclave.

A pressurized water storage tank provides a consistent water supply to the autoclave. Many autoclaves require a pressurized and purified water supply. Some autoclaves have priming pumps for purified water intake, but if the water sup-ply is not pressurized, an air gap may block the water flow. If an air blockage occurs, the operation of autoclave is not possible until the air is removed from the system. The pressurized water storage tank of the Sterifeed system eli-minates the formation of air gaps, and ensures reliable operation of the autoclave.

The Sterifeed system has an LCD display that provides information about the system status, water quality, and the remaining pre-fil-ter life. The water softening modu-le ensures long life of the purifying elements. These design features reduce the operational costs for your laboratory.

The Sterifeed comes with the ne-cessary accessories for connection to an autoclave, and no tools are required!

Sterifeed

Purified water specifications Sterifeed

Purified water resistivity

Purified water conductivity 5-11 μS/cm



Deionization module life N/A

Recovery >30 %


Pressurized storage tank 60 L

Tanks’ dimensions, cm 42x42x60



Specifications

pUre water systems

Consumables


10016Replacement pre-filter set, Sterifeed


Model Part number

Crystal Sterifeed CS-1002

Softeningcartridge

5 μmsediment

filter

ActivatedCarbon

cartridge

1 μmsediment

filter

To autoclave

Pressurizedstoragetank

Inputvalve

Boostpump

Drain

Pressureswitch

Input


(RO module)



21


Flow diagram

22

The Crystal Clinic water purifica-tion system is designed to provide purified water to automated bioc-hemistry analyzers. Purified water ensures long-term, reliable opera-tion from a biochemistry analyzer.

Water produced from the Crystal Clinic has a resistivity of 10

MegaOhm*cm. This exceeds the manufacturers’ requirements for feed water to biochemistry analyzers. A double RO module and pressurized 60L tank ensu-re a stable and reliable supply of water. The Crystal Clinic systems are equipped with a pressure re-gulator. Purified water pressure is

precisely adjusted to the require-ments of the analyzer.

The Crystal Clinic has two reverse osmosis modules and a high-ca-pacity deionization cartridge (DI). The large DI cartridge volume sig-nificantly reduces operating costs.

Water purified by the reverse os-mosis modules is collected in the storage tank.The water is then polished to 10 MegaOhm*cm by the deionization module. The two reverse osmosis modules enable a high flow rate through the perme-able membranes, ensuring that purified water is always available.

A large LCD display provides infor-mation about the system status, water quality, and pre-filter life. The Crystal Clinic has two water quality sensors. The first sensor controls the quality of water filling the storage tank. This is shown on the LCD display while the tank is beeing filled. The second sensor controls the quality of water sup-plied to the Biochemical Analyzer. The Crystal Clinic system automa-tically monitors the performance of the deionization module and alerts the operator when the DI module needs replacement.The DI module and pre-filter can be replaced by the operator. No tools required!

The Crystal Clinic has a Pure water dispense port on the front panel. Pure (Grade 2) water can be used for general laboratory applicati-ons.

Crystal Clinic

Specifications

Purified water specifications Crystal E Clinic

Grade 2 water resistivity >10 MΩ x cm

Grade 2 water conductivity <0.1 μS/cm



Dispense flow to analyzer 4 L/min

Dispense pressure 100 kPa

Deionization module life (high capacity module) 3 m3

Recovery >30 %


Pressurized storage tank 60 L

Tank’ dimensions, cm 42x42x60



Pictures are for reference only. Depending on configuration, some options may not be present

pUre water systems

Toanalyzer

Inputvalve

Inputvalve

Activatedcarbon/5um

pre-filter

Activatedcarbon/5um

pre-filter Boostpump

Drain

Pressureswitch

Pressureswitch

Input

Strainer

Strainer

Reverseosmosismodule

(ROmodule)

Checkvalve

Flowrestrictor



Pressurized storagetank

Deionizationmodule

Pressureregulator

Flowswitch

Pure waterdispense valve

Pure waterdispense

Final point-of-use filter

Consumables


10039Replacement pre-filter set, Crystal Clinic


10113Replacement high-capacity deionization module

„DI Err” message is shown, or dispense water conductivity > 0.1 μS/cm


As required (on average every 3 years)Only for systems equipped with 10102

Components

Part number Accessory Crystal

Clinic

10039 Pre-filter set for chlorine and organics reduction +

Boost pumps +

Reverse osmosis modules +

10101 High capacity deionization module +

10102 UV sterilization module option

Model Part number

Crystal Clinic CL-2810


Flow diagram

Dzerbenes 27, RigaLATVIA, LV – 1006Tel.: +371 67551894, +371 67551993Fax: +371 67551976e-mail: [email protected]

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