jp crude drug test - igz.ch drug analysis catalogue low res.pdf · capsici fructus 20 mg grade...

1

JP C

rud

e D

rug

Test

Product Name Wako Cat. No. Chemical Structural Formula

Achyranthes Root 017-22731

UnidentifiedCrude Drug Pkg. Size

Achyranthes root 5g

Grade Storage

for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.


Aconitum Diester Alkaloids Standard 018-23741

Crude Drug Pkg. Size

Aconiti Rhizoma 0.35 mg

Grade Storage

for the JP Crude Drugs Test (for Purity Determination)

Keep at 2-10℃.


Aconitum Monoester Alkaloids Standard 014-23721


Aconiti Rhizoma 0.25 mg

Grade Storage

for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.


Albiflorin, 98.0+ % (HPLC) 016-22201

C23H28O11 = 480.46CAS No. 39011-90-0


Paeoniae Radix 20 mg

Grade Storage

for the JP General Tests Keep at 2-10℃.


Alisol A, 97.0+ % (HPLC) 018-20681

C30H50O5 = 490.72CAS No. 19885-10-0


Alismatis Rhizoma 20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC) Keep at -20℃.

for other products, please visit the Wako Online Catalog www.e-reagent.com

2

JP C

rud

e Dru

g Test


Amygdalin 017-23571

C20H27NO11 = 457.43CAS No. 29883-15-6


・Armeniacae Semen・Persicae Semen

20 mg

Grade Storage

for the JP Crude Drugs Test (for Assay and TLC)

Keep at 2-10℃.


Asarinin, 98.0+ % (HPLC) 012-24121

C20H18O6 = 354.35CAS No. 133-04-0


asiasarum root 10 mg

Grade Storage

for the JP Crude Drugs Test (for Thin-Layer Chromatography)

Keep at 2-10℃.


(E)-Asarone, 98.0+ % (HPLC) 013-22571

C12H16O3 = 208.25CAS No. 2883-98-9


Perilla Herb 10 mg

Grade Storage



Astragaloside IV, 98.0+ % (HPLC) 015-20691

C41H68O14 = 784.97CAS No. 84687-43-4


Astragali Radix 20 mg

Grade Storage



Atractylenolide III 018-24461

C15H20O3 = 248.32CAS No. 73030-71-4


Atractylodes rhizome 20 mg

Grade Storage


Keep at 2-10℃.

3

JP C

rud

e D

rug

Test


Atractylodin TS (5.0 mg/L in methanol) 015-24471

C13H10O = 182.22CAS No. 55290-63-6


Atractylodes rhizome 1 mL x 5A

Grade Storage



Atropine Sulfate Hydrate, 99.0+ % (HPLC) 011-23731

(C17H23NO3)2・H2SO4・H2O = 694.83CAS No. 5908-99-6


・Belladonnae Radix・Ephedra Herb・Scopolia Rhizome

50 mg

Grade Storage


Keep at 2-10℃.


Arbutin 019-23771

C12H16O7 = 272.25


Uvae Ursi Folium 20 mg

Grade Storage


Keep at 2-10℃.


Artemisia Argyi 018-24221


Artemisiae Folium 10g

Grade Storage


Keep at 2-10℃.


Baicalin, 98.0+ % (HPLC) 024-15691

C21H18O11・H2O = 464.38CAS No. 21967-41-9


Scutellariae Radix 20 mg

Grade Storage



4

JP C

rud

e Dru

g Test


Barbaloin 022-16971

C21H22O9 = 418.39CAS No. 1415-73-2


Aloe 50 mg

Grade Storage


Keep at 2-10℃.


Benz [a] anthracene 023-16761

C18H12 = 228.29CAS No. 56-55-3


Wood-tar creosote 20 mg

Grade Storage



Benzo [a] pyrene 026-16751

C20H12 = 252.31CAS No. 50-32-8



Grade Storage



Benzoylmesaconine Hydrochloride 022-16611

C31H43NO10・HCl = 626.13


Aconiti Rhizoma 5 mg

Grade Storage


Keep at 2-10℃.


Berberine Chloride Hydrate, 95.0 - 102.0 % (calculated on the dehydrous basis)

025-17181

C20H18ClNO4 = 371.81CAS No. 141433-60-5


・Corydalis Tuber・Phellodendri Cortex・Coptidis Rhizoma

20 mg

Grade Storage


5

JP C

rud

e D

rug

Test


Bergenin, 99.0+ % (HPLC) 027-16421

C14H16O9 = 328.27CAS No. 477-90-7


Mallotus japonicus (Thunb.) Muell. Arg. 20 mg

Grade Storage



Bisdemethoxycurcumin 020-16411

C19H16O4 = 308.33CAS No. 33171-05-0


Curcuma longa L. 10 mg

Grade Storage



Bufalin 029-16981

C24H34O4 = 386.52CAS No. 465-21-4


Bufo bufo gargarizans Cantor 20 mg

Grade Storage



(E)-Capsaicin 034-21121

C18H27NO3 = 305.41CAS No. 404-86-4


Capsici Fructus 20 mg

Grade Storage


Keep at 2-10℃.


Chikusetsusaponin IV, 98.0+ % (HPLC) 035-23091

C47H74O18 = 927.08CAS No. 7518-22-1


Panax Rhizome 10 mg

Grade Storage



6

JP C

rud

e Dru

g Test


(E)-Chlorogenic Acid, 98.0+ % (HPLC) 039-20331

C16H18O9 = 354.31CAS No. 327-97-9


Lonicerae Folium Cum Caulis 10 mg

Grade Storage



(E)-Cinnamaldehyde, 98.0+ % (HPLC) 034-21981

This product may be deteriorated phototermally, so please use it in a cool dark place.

C9H8O = 132.16CAS No. 14371-10-9


Cinnamomi Cortex 1 mL x 5

Grade Storage



(E)-Cinnamic Acid 037-22211

This product may be deteriorated phototermally, so please use it in a cool dark place.

C9H8O2 = 148.16CAS No. 140-10-3


Cinnamomi Cortex 20 mg

Grade Storage


Keep at 2-10℃.


Cinobufagin, 98.0+ % (JP method) 037-21971

C26H34O6 = 442.54CAS No. 470-37-1



Grade Storage



Coptisine Chloride, 98.0+ % (HPLC) 038-22001

C19H14NO4Cl = 355.77CAS No. 6020-18-4


Coptidis Rhizoma 10 mg

Grade Storage


7

JP C

rud

e D

rug

Test


Curcumin 031-21991

C21H20O6 = 368.38CAS No. 458-37-7



Grade Storage



Cycloartenyl Ferulate, 98.0+ % (HPLC) 035-22011

C40H58O4 = 602.89CAS No. 21238-33-5


Oryzae Semen 10 mg

Grade Storage



Dehydrocorydaline Nitrate 045-32941

C22H24N2O7 = 428.44CAS No. 13005-09-9


Corydalis Tuber 20 mg

Grade Storage


Keep at 2-10℃.


Demethoxycurcumin 049-31001

C20H18O5 = 338.35CAS No. 22608-11-3



Grade Storage



Eleutheroside B 051-07551

C17H24O9 = 372.37CAS No. 118-34-3


Siberian Ginseng 20 mg

Grade Storage

for the JP Crude Drugs Test (for LC) Keep at 2-10℃.


8

JP C

rud

e Dru

g Test


(E)-Ferulic Acid 067-06091

C10H10O4 = 194.18CAS No. 537-98-4


・Angelicae Radix・Cimicifugae Rhizoma

20 mg

Grade Storage



Fructose, 99.0+ % (HPLC) 060-06081

C6H12O6 = 180.16CAS No. 57-48-7


Rehmanniae Radix 20 mg

Grade Storage



Geniposide, 95.0+ % (qNMR) 073-05891

C17H24O10 = 388.37CAS No. 24512-63-8


Gardeniae Fructus 20 mg

Grade Storage


Keep at 2-10℃.


Gentiopicroside, 98.0+ % (HPLC) 073-05031

C16H20O9 = 356.32CAS No. 20831-76-9


・Gentianae Radix・Gentianae Scabrae Radix

10 mg

Grade Storage



[6]-Gingerol 076-05901

C17H26O4 = 294.39


Zingiberis Rhizoma 20 mg

Grade Storage


Keep at 2-10℃.

9

JP C

rud

e D

rug

Test


Ginsenoside Rc, 98.0+ % (HPLC) 075-05231

C53H90O22 = 1079.27CAS No. 11021-14-0


・Ginseng Radix Rubra・Panacis Ginseng Radix

20 mg

Grade Storage



Ginsenoside Re, 99.0+ % (HPLC) 072-05241

C48H82O18 = 947.15CAS No. 52286-59-6



20 mg

Grade Storage



Ginsenoside-Rg1, 99.0+ % (HPLC) 076-05021

C42H72O14 = 801.01CAS No. 22427-39-0



10 mg

Grade Storage



4'-O-Glucosyl-5-O-methylvisamminol, 98.0+ % (HPLC)

076-05761

C22H28O10 = 452.45CAS No. 84272-85-5


Glehniae Radix cum Rhizoma 5 mg

Grade Storage



Glycyrrhizic Acid, 99.0+ % (HPLC) 070-05161

C42H62O16 = 822.93CAS No. 1405-86-3


Glycyrrhizae Radix 20 mg

Grade Storage



10

JP C

rud

e Dru

g Test


Guaiacol 073-05771

C7H8O2 = 124.14CAS No. 90-05-1



Grade Storage

for the JP General Tests (for Determination) Keep at 2-10℃.


Hesperidin 085-09311

C28H34O15 = 610.56CAS No. 520-26-3


Aurantii Nobilis Pericarpium 10 mg

Grade Storage


Keep at 2-10℃.


Hirsutine 085-09291

C22H28N2O3 = 368.47CAS No. 7729-23-9


Uncariae Uncis Cum Ramulus 5 mg

Grade Storage


Keep at 2-10℃.


Honokiol, 99.0+ % (HPLC) 083-08511

C18H18O2 = 266.33CAS No. 35354-74-6


Magnoliae Cortex 20 mg

Grade Storage



10-Hydroxy-2-(E)-decenoic Acid 081-09271

C10H18O3 = 186.25CAS No. 14113-05-4


Royal jelly 20 mg

Grade Storage


Keep at 2-10℃.

11

JP C

rud

e D

rug

Test


3-(3-Hydroxy-4-methoxyphenyl)-2-(E)-propenoic Acid, 98.0+ % (HPLC)

085-08691

C10H10O4 = 194.18CAS No. 537-73-5


Cimicifugae Rhizoma 20 mg

Grade Storage



Hyodeoxycholic Acid, 98.0+ % (HPLC) 089-09211

C24H40O4 = 392.57CAS No. 83-49-8


・Powdered Swine Bile・Fel Ursi

50 mg

Grade Storage



Hyperoside 087-08651

C21H20O12 = 464.38CAS No. 482-36-0


Crataegi Fructus 10 mg

Grade Storage



Icariin, 99.0+ % (HPLC) 094-05281

C33H40O15 = 676.66CAS No. 489-32-7


Epimedii Herba 20 mg

Grade Storage



Limonin, 98.0+ % (HPLC) 123-06101

C26H30O8 = 470.51CAS No. 1180-71-8


Phellodendri Cortex 10 mg

Grade Storage



12

JP C

rud

e Dru

g Test


Liquiritin, 98.0+ % (HPLC) 129-05341

C21H22O9 = 418.39CAS No. 551-15-5 (anhy)



Grade Storage



Loganin 128-05931

C17H26O10 = 390.38CAS No. 18524-94-2


・Corni Fructus・Lonicerae Folium Cum Caulis

20 mg

Grade Storage


Keep at 2-10℃.


Luteolin, 98.0+ % (HPLC) 125-05441

C15H10O6 = 286.24CAS No. 491-70-3


Chrysanthemi Flos 10 mg

Grade Storage



Magnolol, 98.0+ % (qNMR) 130-16781

C18H18O2 = 266.33CAS No. 528-43-8


Magnoliae Cortex 20 mg

Grade Storage


Keep at 2-10℃.


Manninotriose, 98.0+ % (HPLC) 135-17711

C18H32O16 = 504.44CAS No. 13382-86-0



Grade Storage


13

JP C

rud

e D

rug

Test


l-Menthol, 99.0+ % (JP method) 132-16562

C10H20O = 156.27CAS No. 2216-51-5


Menthae Herba 25g

Grade Storage

for the JP General Tests(for Determination) Keep at 2-10℃.


(E)-2-Methoxycinnamaldehyde,98.0+ % (HPLC)

134-16181

C10H10O2 = 162.19CAS No. 1504-74-1


Cinnamomi Cortex 20 mg

Grade Storage



Myristicin, 98.0+ % (HPLC) 137-16291

C11H12O3 = 192.21CAS No. 607-91-0


Myristica fragrans 20 mg

Grade Storage



Naringin, 99.0+ % (HPLC) 149-07901

C27H32O14 = 616.57CAS No. 10236-47-2


・Rhei Rhizoma・Aurantii Nobilis Pericarpium・Aurantii Pericarpium

20 mg

Grade Storage



Nodakenin, 98.0+ % (HPLC) 148-08571

C20H24O9 = 408.40CAS No. 495-31-8


Peucedani Radix 5 mg

Grade Storage



14

JP C

rud

e Dru

g Test


Osthole, 99.0+ % (HPLC) 151-02641

C15H16O3 = 244.29CAS No. 484-12-8


Cnidii Monnieri Fructus 20 mg

Grade Storage



Paeoniflorin, 98.0+ % (HPLC) 164-21731

C23H28O11 = 480.46CAS No. 23180-57-6


Paeoniae Radix 10 mg

Grade Storage



Paeonol, 98.0+ % (qNMR) 164-24891

C9H10O3 = 166.17CAS No. 552-41-0


Paeoniae Moutan Cortex 10 mg

Grade Storage


Keep at 2-10℃.


Palmatine Chloride, 99.0+ % (HPLC) 165-22481

C21H22ClNO4 = 387.86CAS No. 10605-02-4


Phellodendri Cortex 20 mg

Grade Storage



Perillaldehyde 161-24161

C10H14O = 150.22CAS No. 2111-75-3


Perillae Herba 30 mg× 5A

Grade Storage


Keep at 2-10℃.

15

JP C

rud

e D

rug

Test


Plantago Seed 168-24171


Plantaginis Semen 10g

Grade Storage



Powdered Swine Bile 168-24311


Fel Ursi 1g

Grade Storage



(±)-Praeruptorin A, 98.0+ % (HPLC) 165-25281

C21H22O7 = 386.40CAS No. 73069-25-7



Grade Storage



Puerarin, 98.0+ % (HPLC) 165-22001

C21H20O9 = 416.38CAS No. 3681-99-0


Puerariae Radix 20 mg

Grade Storage



Resibufogenin, 98.0+ % (JP method) 185-02681

C24H32O4 = 384.51CAS No. 465-39-4



Grade Storage


Keep at 2-10℃.


16

JP C

rud

e Dru

g Test


Rhein, 98.0+ % (HPLC) 180-02251

C15H8O6 = 284.22CAS No. 478-43-3


Rhei Rhizoma 10 mg

Grade Storage



Rhynchophylline 188-02671

C22H28N2O4 = 384.47CAS No. 76-66-4


Uncariae Uncis Cum Ramulus 10 mg

Grade Storage


Keep at 2-10℃.


Rosmarinic Acid 181-02661

C18H16O8 = 360.31CAS No. 20283-92-5


Perillae Herba 20 mg

Grade Storage


Keep at 2-10℃.


Saikosaponin a 192-16281

C42H68O13 = 780.98CAS No. 20736-09-8


Bupleuri Radix 25 mg

Grade Storage


Keep at 2-10℃.


Saikosaponin b2 199-16171

C42H68O13 = 780.98CAS No. 58316-41-9



Grade Storage


Keep at 2-10℃.

17

JP C

rud

e D

rug

Test


Saikosaponin d 199-16311

C42H68O13 = 780.98CAS No. 20874-52-6



Grade Storage



Schizandrin, 99.0+ % (HPLC) 197-13551

C24H32O7 = 432.51CAS No. 7432-28-2


Schisandrae Fructus 20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC) Keep at RT.


Scopoletin, 98.0+ % (HPLC) 191-16751

C10H8O4 = 192.17CAS No. 92-61-5


Artemisiae Folium 20 mg

Grade Storage



Sesamin, 98.0+ % (HPLC) 197-16231

C20H18O6 = 354.35CAS No. 607-80-7


Sesami Semen 20 mg

Grade Storage



[6]-Shogaol 192-16161

C17H24O3 = 276.37CAS No. 555-66-8


Zingiberis Siccatum Rhizoma 10 mg

Grade Storage


Keep at 2-10℃.


18

JP C

rud

e Dru

g Test


Sodium Glycocholate, 98.0+ % (HPLC) 197-15491

C26H42NNaO6 = 487.60CAS No. 863-57-0


Fel Ursi 20 mg

Grade Storage



Sodium Tauroursodeoxycholate, 98.0+ % (HPLC)

190-15501

C26H44NNaO6S = 521.69CAS No. 35807-85-3


Fel Ursi 20 mg

Grade Storage



Stachyose, 99.0+ % (HPLC) 195-16911

C24H42O21 = 666.58CAS No. 470-55-3



Grade Storage



Swertiamarin, 99.0+ % (HPLC) 191-13951

C16H22O10 = 374.34CAS No. 17388-39-5


Swertia Herb 20 mg

Grade Storage


Product Name Chemical Structural Formula

Theophylline

C7H8N4O2 = 180.16CAS No. 58-55-9

Crude Drug

Swertia Herb

Grade Storage

for the JP Crude Drugs Test (for Determination) Keep at 2-10℃.

Wako Cat. No.

203-18281 209-18283

Pkg. Size

100mg 1 g

19

JP C

rud

e D

rug

Test


Umbelliferone, 98.0+ % (HPLC) 212-01461

C9H6O3 = 162.14CAS No. 93-35-6


Artemisiae Folium 20 mg

Grade Storage



Wogonin, 98.0+ % (HPLC) 236-02321

C16H12O5 = 284.26CAS No. 632-85-9



Grade Storage


Functional group Packing material name Column SizeWako Cat. No.

Purity of silica gelD (DuPont type) W (Waters type)

Separation mode: Partition

ODS (C30) Navi C30-5 4.6 φ x 150 mm 237-61191 233-61193

High purity

ODS (C18) Navi C18-5 4.6 φ x 150 mm 231-60511 237-60513

Wakosil-II 5C18 4.6 φ x 150 mm 230-51811 236-51813

Wakosil-II 5C18 AR 4.6 φ x 150 mm 238-50751 234-50753

Wakosil-II 5C18 HG 4.6 φ x 150 mm 238-51111 234-51113

Wakosil-II 5C18 RS 4.6 φ x 150 mm 238-51471 234-51473

Wakosil 5C184.6 φ x 150 mm 233-53981 239-53983

Normal4.6 φ x 250 mm 237-54001 233-54003

Wakosil 5C18-200 4.6 φ x 250 mm 235-55521 231-55523

Ph (phenyl) Wakosil 5Ph 4.6 φ x 250 mm 230-57411 236-57413

Product Name Package Size Wako Cat. No. Grade

Silicagel 70 Plate Wako 100 sheets ( 5 × 20 cm) 197-08384

for Thin-Layer Chromatography

Silicagel 70 F254 Plate Wako 100 sheets ( 5 × 20 cm) 197-08404

Silicagel 70 PF254 Plate Wako 10 sheets (20 × 20 cm) 195-12871

Silicagel 70 FM Plate Wako 100 sheets ( 5 × 20 cm) 194-08394

TLC plates used in analysis listed in this catalog

HPLC columns used in analysis listed in this catalog


20

Cru

de D

rug

TestProduct Name Wako Cat. No. Chemical Structural Formula

Achyranthes Root 015-22271

unidentified


Achyranthis Radix 5 g

Grade Storage

for Crude Drugs Determination Keep at 2-10℃.

Example analysis

TLC

Sample: Dissolve the 2 g in 10 mL of water. → Shake for 10 min. → Add 5 mL of 1-Butanol. → Shake for 10 min. → The supernatant after centrifugation.

Injection: 10 μLThin-layer plate: Silicagel plate for TLCDeveloping solvent: Ethyl Acetate 5 + Water 1 + Formic Acid 1 (volume ratio) or

1-Propanol 4 + Ethyl Acetate 4 + Water 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Spray diluted sulfuric acid.→Dry at 105℃ for 5 min.

→ Visual judgment.


Aconitine Standard, 99.0+ % (HPLC) 019-14621

C34H47NO11 = 645.74CAS No. 302-27-2



Grade Storage


Example analysis

TLC HPLC

Sample: 2 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: The upper layer of [Butyl Acetate 8 + 1-Propanol1 + Ammonia

Solution 1 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: ① Spray Dragendorff's Reagent 1) →Visual judgment.

②UV exposure

1) Bismuth (III) Nitrate Basic 0.17 g + Acetic Acid 2 mL + Water 8 mL → Shake → Add Potassium Iodide Solution (400 g/L) 10 mL → Shake

Sample: 1 mg/mL (Acetonitrile)Injection: 10 μLColumn: Wakosil-II 5C18 AR, 4.6 mm φ × 15 cm

[Wako Cat. No.: 234-50753 (W), 238-50751 (D)] *Column Temperature: Room temperatureEluent: 0.05 mol/L Phosphate buffer 1) 68 + Acetonitrile 32 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm

1) [Disodium Hydrogen Phosphate 7.1 g + Water （→1 L)] + [Potassium Dihydrogen Phosphate 6.8 g + Water （→1 L)] (→ pH 7.4).


Acteoside Standard　【Verbascoside】, 98.0+ % (HPLC)

013-24531

C29H36O15 = 624.59CAS No. 61276-17-3


Cistanche Herb 10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 10 mg/2 mL (Methanol) Sample spotted: 20 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 20 + Methanol 3 + Water 2 (volume ratio)Distance spot travels: abt. 7 cmDetection method: Dry at RT. → Spray 2,6-Dibromo-N-Chloro-p-benzoquinone

Monoimine Solution 1). →Leave it in ammonia gas.→ Visual judgment.

1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.5 g + Methanol→100 mL

Sample: 2 mg/10 mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18HG，4.6 mm φ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Phosphoric Acid 85 + Acetonitrile 15 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 330 nm

* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)

21

Cru

de

Dru

g Te

stProduct Name Wako Cat. No. Chemical Structural Formula

Alisol B Acetate, 98.0+ % (HPLC) 018-13231

C32H50O5 = 514.74CAS No. 26575-95-1



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 55 + Acetone 35 + Hexane 10 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Dry at 100℃ for 10 min. →Allow to cool

→Spray Sulfuric Acid‐Ethanol (99.5) solution (1+20) → Dry at RT → Heat at 200℃ for 10 min. → Visual judgment.

Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18，4.6 mm φ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room temperatureEluent: Acetonitrile 80 + Water 20 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203 nm


Alisol B Standard, 99.0+ % (HPLC) 010-13431

C30H48O4 = 472.70CAS No. 18649-93-9



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 55 + Acetone 35 + Hexane 10 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray sulfuric acid (10 vol%)→Heat at 120℃ for 5 min.

Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18，4.6 mmφ x 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column temperature: Room TemperatureEluent: Acetonitrile 75 + Water 25 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203nm


Alkannin Standard, 99.0+ % (HPLC) 013-19901

C16H16O5 = 288.30CAS No. 517-88-4


Lithospermum erythrorhizon 10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Acetone) Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt.10 cmDetection method: UV exposure and Visual judgment

Sample: 1 mg/mL (Methanol)Injection: 5 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 2 vol% Phosphoric Acid solution 25 + Methanol 75（volume ratio）Flow rate: 1.0 mL/minDetector: UV 273nm


22

Cru

de D

rug


Arctiin Standard, 98.0+% (HPLC) 019-24631

C27H34O11 = 534.55CAS No. 20362-31-6


Arctium lappa L. 20 mg

Grade Storage


Example analysis

HPLC

Sample: 5 mg/5 mL (Eluent)Injection: 10μlColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Water 75 + Acetonitrile 25 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 278nm


Artemisia Argyi 018-24221

Unidentified


Artemisiae Folium 10 g

Grade Storage


Keep at 2-10℃.

Example analysis

TLC

Sample: Add the 0.5 g in 5 mL of [Methanol 3 + Water 2 (volume ratio)]. → Shake for 10 min. → Centrifuged supernatant.

Injection: 10 μLThin-layer plate: Octadecylsilanized silica gel for TLCDeveloping solvent: Methanol 4 + Water 1 (volume ratio) Distance spot travels: abt. 7 cmDetection method: Dry at RT. →Spray diluted sulfuric acid.→Dry at 105℃ for 5

min. →UV exposure (dominant wavelength: 365 nm). → Green fluorescing spots at around 0.3 and 0.4 of Rf value are confirmed as identification of eupatilin and jaceosidin.


Atractylodin Standard, 98.0+ % (HPLC) 011-21531

C13H10O = 182.22CAS No. 55290-63-6


Atractylodis Lanceae 20 mg

Grade Storage

for Crude Drugs Determination Keep at -80℃.

Example analysis

TLC HPLC

Sample: 20 μg/mL (Hexane)Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Hexane 7 + Acetone 1 (volume ratio) Distance spot travels: abt. 10 cmDetection method: Dry at RT. →Spray p-dimethylaminobenzaldehyde solution.

→ Heat at 105℃ for 5 min. →Visual judgment

Sample: 20 μg/mL (Methanol)Injection: 20 μLColumn: Wakosil-II 5C18 RS, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: [Water 55 + Phosphoric Acid 1 (volume ratio)] 33 + Acetonitrile 67 (volume ratio)Flow rate: 0.8 mL/min Detector: UV 336 nm


23

Cru

de

Dru

g Te


Aucubin Standard, 97.0+ % (HPLC) 016-10351

C15H22O9 = 346.33CAS No. 479-98-1


Plantaginis Semen 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: The lower layer of [Chloroform 26 + Methanol 14

+ Water 5 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p‐Anisaldehyde 1g + Ethanol (99.5) 50 mL

+ Sulfuric Acid (1+5) 50 mL] → Heat → Visual judgment

Sample: 1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mm φ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room TemperatureEluent: Acetonitrile 3 + Water 97 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 206 nm


Baicalein Standard, 98.0+ % (HPLC) 027-07751

C15H10O5 = 270.24CAS No. 491-67-8



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 2 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetic Acid 10 + Ethyl Acetate 70 + Methanol 30

+ Water 5 (volume ratio) Distance spot travels: abt. 10 cmDetection method: UV exposure (dominant wavelength: 254 nm)

Sample: 0.1 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18, 4.6 mmφ × 15 cm

[Wako Cat. No.: 236-51813 (W), 230-51811 (D)] *Column temperature: a constant temperature aroung room temperature Eluent: 0.1 mol/L Phosphate buffer (pH2.1) 1) 40 + Methanol 60 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 280 nm1) Potassium Dihydrogen Phosphate 2.72 g + Water 180 mL → Adjust the pH to 2.1 with Phosphoric Acid. + Water (→ 200 mL).


Bufotalin Standard, 99.0+ % (HPLC) 024-10691

C26H36O6 = 444.57CAS No. 471-95-4



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetone 3 + Chloroform 3 + Cyclohexane 4 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfuric Acid (1+5) Solution (10 g/L)

→ Heat at 105℃ for 5 min.

Sample: 0.5 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room temperatureEluent: Acetonitrile 7 + Water 13 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 280 nm


24

Cru

de D

rug


Calycosin 7-O-Glucoside Standard, 98.0+ % (HPLC)

039-22271

C22H22O10 = 446.4CAS No. 20633-67-4


Astragali Radix 10 mg

Grade Storage


Example analysis

HPLC

Sample: 0.2 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18 RS, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃ Eluent: Water 850 + Acetonitrile 150 + Acetic Acid 1 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 254 nm


Carbazochrome Sodium Sulfonate Trihydrate, 99.0+ % (Calculated on the dehydrous basis)

039-21551

C10H11N4NaO5S・3H2O = 376.32CAS No. 51460-26-5


Gardeniae Fructus 500 mg

Grade Storage

for Crude Drugs Determination Keep at RT.

Example analysis

HPLC

Sample: 0.5 mg/mL (Water)Injection: 10 μLColumn: ODS (particle size: 7 μm), 4.6 mmφ × 25 cm Column Temperature: 40℃ Eluent: 10 mmol/L Phosphate buffer 1) 925 + Ethanol (95) 75 (volume ratio)

+ Phosphoric Acid (→pH 3.0)Detector: UV 360 nm

1) Ammonium dihydrogen phosphate 1.2 g + Water 1,000 mL


Catalpol Standard, 98.0+ % (HPLC) 032-10551

C15H22O10 = 362.33CAS No. 2415-24-9



Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: The lower layer of [Chloroform 65 + Methanol 35

+ Water 10 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray Sulfuric Acid (1+10). →Heat →Visual judgment

Sample: 0.5 mg/mL (Eluent)Injection:10 μLColumn: Wakosil 5C18, 4.6 mmφ x 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 35℃Eluent: Acetonitrile 1 + Water 99 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 203 nm


25

Cru

de

Dru

g Te


Cinobufotalin Standard, 98.0+ % (HPLC) 035-13721

C26H34O7 = 458.55CAS No. 1108-68-5



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetone 3 +Chloroform 3 +Cyclohexane 4 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfric Acid (1+5) Solution (1 w/v%)

→ Heat at 105℃ for 5 min.→Visual judgment.

Sample: 1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm



Corosolic Acid Standard, 98.0+ % (HPLC) 030-20741

C30H48O4 = 472.70CAS No. 4547-24-4


Lagerstroemia speciosa 10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Chloroform 10 + Methanol 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Spray Sulfuric Acid-Ethanol (99.5) solution (1+20).

→Dry at RT.→Heat at 130℃ for 10 min.→Visual judgment


[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Trifluoroacetic Acid 35 + Acetonitrile 65 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 210nm


Corydaline Standard, 99.0+ % (HPLC) 036-14971

C22H27NO4 = 369.45CAS No. 518-69-4


Corydalis Tuber 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent：Acetone 3 +Chloroform 3 +Cyclohexane 4（volume ratio）Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfuric Acid (1+5) Solution (1 w/v%)

→ Heat at 105℃ for 5 min. →Visual judgment

Sample: 1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm



26

Cru

de D

rug


Dehydrocostus Lactone Standard, 98.0+ % (HPLC)

042-32711

C15H18O2 = 230.3CAS No. 477-43-0


Saussureae Radix 20mg

Grade Storage


Example analysis

TLC HPLC

Sample: 2 mg/ 2 mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394] Developing solvent: Acetone 1 + Ethyl Acetate 2 + Hexane 7 (volume ratio) Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfuric Acid (1+5) Solution (10 g/L)

→Heat at 105℃ for 5 min.→Visual judgment

Sample: 0.2 mg/mL (Eluent) Injection：10 μL Column: Wakosil-Ⅱ 5C18HG, 4.6 mmφ×15 cm,

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃Eluent: Acetonitrile 60 + Water 40 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 215nm


Dihydrocapsaicin Standard, 99.0+ % (HPLC)

045-28911

C18H29NO3 = 307.43CAS No. 19408-84-5


Capsici Fructus 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 10 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Diethyl Ether 19 + Methanol 1 (volume ratio) Distance spot travels: abt. 12 cmDetection method: Spray 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine

Solution 1). →Leave it in ammonia gas.→ Visual judgment.

1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.1 g + Methanol 20 mL.

Sample: 0.2 mg/mL (Methanol)Injection: 20 μL Column: Wakosil 5Ph，4.6 mmφ × 25 cm

[Wako Cat. No.: 236-57413 (W), 230-57411 (D)] *Column Temperature: 40℃ Eluent: 0.1 vol% Phosphoric Acid 50 + Acetonitrile 50 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 281 nm


5,7-Dimethoxyflavone Standard, 98.0+ % (HPLC)

040-32991

C17H14O4 = 282.29CAS No. 21392-57-4


Kaempferia parviflora, 20 mg

Grade Storage


Example analysis

HPLC

Sample: 0.1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 25 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃ Eluent: Acetonitrile 55 + Water 45 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 260 nm


27

Cru

de

Dru

g Te


2,6-Dimethoxyphenol Standard 047-30681

(CH3O)2C6H3OH = 154.16CAS No. 91-10-1


- 5 g

Grade Storage


Example analysis


Dimethylesculetin Standard 042-32331

C11H10O4 = 206.19CAS No. 120-08-1


Artemisiae Capillari Flos 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394] Developing solvent: Hexane 50 + Acetone 50 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (Dominant wavelength: 365 nm)

Sample: 0.1 mg/mL (Acetonitrile)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃ Eluent: Water 80 + Acetonitrile 20 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 230 nm


Echinacoside Standard, 98.0+ % (HPLC) 056-08601

C35H46O20 = 786.73CAS No. 82854-37-3


Cistanche Herb 10 mg

Grade Storage


Example analysis

HPLC

Sample: 5 mg/ 20 mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG，4.6 mm φ × 25 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Phosphoric acid 85 + Acetonitrile 15 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 330 nm


28

Cru

de D

rug


Emetine Dihydrochloride Standard, 98.0+ % (HPLC)

059-08211

C29H40N2O4・2HCl = 553.56CAS No. 316-42-7


Carapichea ipecacuanha 20mg

Grade Storage


Example analysis

HPLC

Sample: 10mg/10ml (Eluent) Injection: 10 μLColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃Eluent: Sodium 1-heptanesulfonate Solution 1) 50 + Methanol 50(volume ratio) Flow rate: 1.0 mL/minDetector: UV 283nm

1) Sodium 1-heptanesulfonate 2.0g＋Water 500mL＋Acetic Acid (→pH4.0).


Epihesperidin Standard, 99.0+ % (HPLC) 085-08211

C28H34O15 = 610.56CAS No. 369593-42-0


Aurantii Nobilis Pericarpium 10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 125 μg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 8 + Ethanol (99.5) 2 + Water 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure (254 nm)

Sample: 125 μg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18，4.6 mm φ × 15 cm

[Wako Cat. No.: 236-51813 (W), 230-51811 (D)] *Column Temperature: 50℃Eluent: Water 82 + Acetonitrile 18 + Acetic Acid 1 (volume ratio) Flow rate: 0.7 mL/minDetector: UV 285 nm


Ergosterol Standard, 98.0+ % (HPLC) 052-04921

C28H44O = 396.65CAS No. 57-87-4


Polyporus 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Chloroform)Sample spotted: 5 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetone 1 + Chloroform 1 + Toluene 10 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure

Sample: 0.4 mg/mL (Chloroform 1 + Methanol 1 (volume ratio))Injection: 10 μLColumn: Wakosil 5C18, 4.6 mm φ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: RTEluent: MethanolFlow rate: 1.0 mL/minDetector: UV 254 nm


29

Cru

de

Dru

g Te


β-Eudesmol Standard, 98.0+ % (HPLC) 056-05161

C15H26O = 222.37CAS No. 473-15-4


Atractylodis Lanceae 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Acetone 1 + Ethyl Acetate 2 + Hexane 7 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)-Sulfuric Acid (1+5) Solution (10 g/L).

→Heat at 105℃ for 5 min.

Sample: 0.5 mg/mL (Eluent)Injection: 10 μL Column: Navi C30-5, 4.6 mm φ × 15 cm

[Wako Cat. No.: 233-61193 (W), 237-61191 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 60 + Water 40 (volume ratio)Detector: UV 203 nm


Evodiamine Standard, 98.0+ % (HPLC) 051-08411

C19H17N3O = 303.36


Evodiae Fructus 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Chloroform)Injection: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Hexane 50 + Ethyl Acetate 50 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (dominant wavelength: 254 nm)

Sample: 0.2 mg/mL (Acetonitrile)　Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃ Eluent: Acetonitrile 50 + 0.01 vol% Phosphoric Acid 50 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 270 nm


Geniposidic Acid Standard, 98.0+ % (HPLC)

078-05841

C16H22O10 = 374.34CAS No. 27741-01-1


・Gardeniae Fructus・Plantaginis Semen

20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol) Sample spotted: 5 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Chloroform 13 + Methanol 7 + Water 1 (volume ratio)Distance spot travels: abt. 10 cm Detection method: ①UV exposure at 254 nm

②Spray [Vanillin 1 g + Ethanol (99.5) 50 mL + Sulfuric Acid(1+5) 50 mL]→ Heat at 105℃) →Visual judgment.

Sample: 0.2 mg/mL (Eluent)Sample spotted: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 35℃ Eluent: 2 vol% Acetic Acid 95 + Acetonitrile 5 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 240 nm


30

Cru

de D

rug


[6]-Gingerol Standard, 98.0+ % (HPLC) 071-06051

C17H26O4 = 294.39



Grade Storage


Example analysis

TLC HPLC

Sample: 0.5 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 1 + Hexane 1 (volume ratio) Distance spot travels: abt. 10 cm Detection method: Dry at RT.→ Spray 4-dimethylaminobenzaldehyde Solution 1).

→Heat at 105℃ for 5 min. →Allow to cool. → Visual judgment.

1) 4-dimethylaminobenzaldehyde 10g + chilled [Sulfuric Acid 90 mL + Water 10 mL]


[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃ Eluent: Water 620 + Acetonitrile 380 + Phosphoric Acid 1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 282 nm


[8]-Gingerol Standard, 98.0+% (HPLC) 079-06231

C19H30O4 = 322.44CAS No. 23513-08-8



Grade Storage


Example analysis

HPLC

Sample: 5 mg/5 mL (Eluent) Injection: 10 μLColumn: Wakosil-II5C18HG, 4.6 mmφ×15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃Eluent: Water 50 + Acetonitrile 50 + Acetonitrile 0.1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 280nm


[10]-Gingerol Standard, 98.0+% (HPLC) 072-06221

C21H34O4 = 350.49CAS No. 23513-15-7



Grade Storage


Example analysis

HPLC

Sample: 5 mg/5 mL (Eluent) Injection: 10 μLColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃Eluent: Water 40 + Acetonitrile 60 + Acetonitrile 0.1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 280nm


31

Cru

de

Dru

g Te


Ginsenoside-Rb1 Standard, 98.0+ % (HPLC)

079-02191

C54H92O23 = 1109.29CAS No. 41753-43-9



10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: The lower layer of [Chloroform 13 + Methanol 7

+ Water 2 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p-Anisaldehyde 1 g + Ethanol (99.5) 50 mL + Sulfuric Acid

(1+5) 50 mL] → Heat →Visual judgment.

Sample: 0.5 mg/mLInjection: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 35 + Water 65 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203 nm


Ginsenoside-Rd Standard, 99.0+ % (HPLC)

072-03301

C48H82O18 = 947.15CAS No. 52705-93-8



20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 10 μL Thin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: The lower layer of [Chloroform 13 + Methanol 7

+ Water 2 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p-methoxybenzaldehyde 1 g + Ethanol (99.5) 50 mL

+ Sulfuric Acid (1+5) 50 mL] → Heat → Visual judgment.

Sample: 0.5 mg/mL (Eluent) Injection: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm



Glabridin, 97.0+ % (HPLC) 070-04821

C20H20O4 = 324.37CAS No. 59870-68-7



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Ethanol)Injection: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404] Developing solvent：Hexane 5 + Diethyl Ether 2 + Ethanol (99.5) 2 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (254nm)


[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 40℃ Eluent: 2 vol% Acetic Acid 40 + Acetonitrile 60 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 282 nm


32

Cru

de D

rug


Glycocholic Acid Standard, 98.0+ % (HPLC)

072-05361

C26H43NO6 = 465.62CAS No. 475-31-0


Fel Ursi 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 10 + Acetic Acid 10 + Water 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Spray Sulfuric Acid (1+18) → Heat at 105℃ for 10 min.

→ Visual judgment

Sample: 0.45 mg/mL (Methanol) 　Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 67 + Acetonitrile 33 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 200 nm

1) Potassium Dihydrogen Phosphate1.36g + Water 450 mL + Phosphoric Acid (→pH 3.0) + Water (→500 mL)


Glycohyodeoxycholic Acid Standard, 98.0+ % (HPLC)

073-06251

C26H43NO5 = 449.62CAS No. 13042-33-6


・Powdered Swine Bile・Fel Ursi

50mg

Grade Storage


Example analysis

TLC HPLC

Sample: 10 mg/5 mL (Methanol)Sample spotted: 2 μLThin-layer plate: Silicagel 60 Plate, 20 x 5 cm Developing solvent: Ethyl Acetate 12 + Methanol 2 + Formic Acid 1 (volume ratio) Distance spot travels: abt. 7 cm Detection method: Dry at RT. →Spray [Vanillin 3 g + Ethanol (99.5) 100 mL + Sulfuric

Acid 0.5 mL]→ Heat at 105℃ for 10 min.→Visual judgment.

Sample: 5 mg/ 5 mL (Methanol) 　Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm


1) Potassium Dihydrogen Phosphate 1.42g + Water 400 mL + Phosphoric Acid (→pH 3.0) + Water (→500 mL)


Glycyrrhizic Acid Standard, 99.0+ % (HPLC)

071-02271

C42H62O16 = 822.93CAS No. 1405-86-3



Grade Storage


Example analysis

TLC HPLC

Sample: 2 mg/mL (Ethanol (50 vol%))Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394] Developing solvent: 1-Butanol 7 + Acetic Acid 1 + Water 2 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (254 nm)

Sample: 0.25 mg/mL (Ethanol (50 vol%))Injection: 20 μLColumn: Wakosil 5C18-200, 4.6 mmφ × 25 cm

[Wako Cat. No.: 237-55503 (W), 231-55501 (D)] *Column Temperature: 40℃ Eluent: Acetic Acid (6.7 vol%) 1) 3 + Acetonitrile 2 (volume ratio) Flow rate: 0.8 mL/min Detector: UV 254 nm

1) Acetic Acid 20 mL + Water (→300 mL).


33

Cru

de

Dru

g Te


Gomisin A Standard, 98.0+ % (HPLC) 077-04951

C23H28O7 = 416.46CAS No. 58546-54-6


Schisandrae Fructus 10mg

Grade Storage


Example analysis

TLC HPLC

Sample: 2.5 mg/mL (Acetonitrile)Sample spotted: 1 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 10 + Hexane 10 + Acetic Acid 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV 254 nm

Sample: 1 mg/mL (Acetonitrile)Injection: 5 μLColumn: Wakosil-II 5C18 HG，4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Methanol 60 + Water 40 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm


Gomisin N Standard, 98.0+ % (HPLC) 074-04961

C23H28O6 = 400.46CAS No. 69176-52-9


Schisandrae Fructus 10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 2.5 mg/mL (Acetonitrile)Sample spotted: 1 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 10 + Hexane 10 + Acetic Acid 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV 254 nm

Sample: 1 mg/mL (Acetonitrile)Injection: 5 μLColumn: Wakosil-II 5C18 HG，4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Methanol 75 + Water 25 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm


Hypaconitine, 98.0+ % (HPLC) 084-07061

C33H45NO10 = 615.72CAS No. 6900-87-4


Aconiti Rhizoma 20mg

Grade Storage

for Crude Drugs Determination Keep below 25℃.

Example analysis

TLC HPLC

Sample: 5 mg/ 5mL (Methanol) Injection: 20 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure

Sample: 1 mg/mL (Methanol) Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.05 mol/L Phosphate buffer (pH 2.4) 1) 55 + Tetrahydrofuran 7

+ Acetonitrile 2 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm

1) Potassium Dihydrogen Phosphate1.36 g + Water 180 mL →Adjust the pH to 2.4 with Phosphoric Acid + Water （→200 mL).


34

Cru

de D

rug


Isoacteoside Standard, 98.0+ %（HPLC) 095-06671

C29H36O15 = 624.59CAS No. 61303-13-7


Cistanche Herb 10mg

Grade Storage


Example analysis

TLC HPLC

Sample: 10 mg/2 mL (Methanol)Sample spotted: 20 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 20 + Methanol 3 + Water 2 (volume ratio)Distance spot travels: abt. 7 cmDetection method: Dry at RT. → Spray 2,6-Dibromo-N-Chloro-p-benzoquinone

Monoimine Solution 1). →Leave it in ammonia gas. → Visual judgment.

1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.5 g + Methanol (→100 mL)

Sample: 2 mg/10 mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18HG，4.6 mm φ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Phosphoric Acid 85 + Acetonitrile 15 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 330 nm


Isofraxidine Standard, 99.0+ % (HPLC) 099-03651

C11H10O5 = 222.19CAS No. 486-21-5


Acanthopanax Root Bark 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 2 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetic Acid 1 + 1-Butanol 5 + Water 2 (volume ratio) Distance spot travels: abt. 10 cm Detection: UV exposure

Sample: 0.2 mg/mL (Methanol)Injection: 5 μLColumn: Wakosil 5C18, 4.6 mm φ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 40℃ Eluent: 0.1% Acetic Acid 80 + Acetonitrile 20 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 354 nm


(Z)-Ligustilide Standard Solution (0.1 mg/mL Methanol Solution)

121-05801

C12H14O2 = 190.24CAS No. 4431-01-0


Angelicae Radix 500 μL x 5A

Grade Storage


Example analysis

TLC HPLC

Sample spotted: 1 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 1 + Hexane 1 (volume ratio)Distance spot travels: abt. 10 cm Detection: UV exposure

Injection: 20 μLColumn: Wakosil-II 5C18 RS, 4.6 mm φ × 15 cm

[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: Phosphoric Acid (1+55) 40 + Acetonitrile 60 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 326 nm


35

Cru

de

Dru

g Te


Magnoflorine Standard, 95.0+% (HPLC) 138-17201

C20H24NO4 = 342.41CAS No. 2141-09-5


Magnoliae Flos 5 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 5 + Acetone 3 + Water 1 + Formic Acid 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Air drying →Spray Dragendorff's Reagent 1) →Visual judgment.

1) Bismuth Nitrate Basic 0.85 g + Acetic Acid 10 mL + Water 40 mL (Dissolve by shaking vigorously)(= Soln. A). Potassium Iodide 8 g + Water 20 mL (= Soln. B). Just before use, mix equal part of Soln. A and Soln. B. Take the 4 mL of the mixture and add 20 mL of 1 mol/L Acetic Acid.

Sample: 0.2 mg/mL (Methanol) Injection: 20 μLColumn: Wakosil-II 5C18 RS, 4.6 mm φ × 15 cm

[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: SDS Solution 1) 65 + Acetonitrile 35 + Phosphoric Acid 0.1 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 270 nm 1) Sodium Dodecyl Sulfate 1 g + Water (→130 mL).


Magnoflorine Iodide Standard, 95.0+% (qNMR)

138-17941

C20H24INO4 = 469.31CAS No. 4277-43-4


Magnoliae Flos 10mg

Grade Storage


Example analysis

TLC HPLCSample: 5 mg + 1 mL of [Methanol 1 + Water 1] → ultrasonic irradiation + 2 mL of

[Methanol 1 + Water 1]Sample spotted: 10 μLThin-layer plate: Silicagel 60, 20 x 5 cm Developing solvent: Ethyl Acetate 5 + Acetone 3 + Water 1 + Formic Acid 1 (volume ratio)Distance spot travels: abt. 7 cmDetection method: Dry at RT.→Spray Dragendorff Solution 1). → Dry at RT.→Spray

Sodium Nitrite Solution 2)→ Visual judgment.

1) Bismuth Subnitrate 0.85g + Acetic Acid 10 mL +Water 40 mL (Beat Up) → [Soln. C]. Potassium Iodide 8 g + Water 20 mL → [Soln. D]. Soln. C 2 mL + Soln. D 2 mL + 1 mol/L Acetic Acid 20 mL (Prepare these solutions just before use.)

2) Sodium Nitrite 10 g + Water (→ 100 mL)

Sample: 5 mg + 5 mL of [Methanol 1 + Water 1] → Ultrasonic Irradiation → Add [Methanol 1 + Water 1] to prepare the 10 mL soln.

Injection: 10 μLColumn: Wakosil-II 5C18 RS, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: Sodium Lauryl Sulfate 5 g + Acetonitrile 350 mL + Water 650 mL

+ Phosphoric Acid 1 mL (volume ratio) Flow rate: 1.0 mL/minDetector: UV 303 nm


Mesaconitine, 98.0+% (HPLC) 130-12261

C33H45NO11 = 631.71CAS No. 2752-64-9



Grade Storage

for Crude Drugs Determination Keep below 25℃.

Example analysis

TLC HPLC

Sample: 2 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method : UV exposure

Sample: 1 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mm φ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.05 mol/L Phosphate buffer (pH 2.4) 1) 90 + Tetrahydrofuran 10 (volume ratio)Flow rate: 1.2 mL/minDetector: UV 236 nm

1) Potassium Dihydrogen Phosphate1.36 g + Water 180 mL→ Adjust the pH to 2.4 with Phosphoric Acid + Water (→ 200 mL).


36

Cru

de D

rug


Neohesperidin Standard, 98.0+% (HPLC) 142-09191

C28H34O15 = 610.56CAS No. 13241-33-3


immature orange 10mg

Grade Storage


Example analysis

TLC HPLC

Sample: 2 mg / 2 mL (Methanol) Sample spotted: 20 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384] Developing solvent: Ethyl Acetate 7 + 1-Propanol 5 + Water 4 + Acetic Acid 1 (volume

ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT.→Spray 2,6-Dibromo-N-Chloro-1,4-benzoquinone

Monoimine Solution 1). →Leave it in ammonia gas.→ Visual judgment.

1) 2,6-Dibromo-N-Chloro-1,4-benzoquinone Monoimine 0.5g + Methanol (→100 mL).

Sample: 2 mg / 10mL (Methanol)Injection: 10 μLColumn: Wakopak Navi C18-5，4.6 mm φ × 15 cm

[Wako Cat. No.: 237-60513 (W),231-60511 (D)] *Column Temperature: 40℃Eluent: Water 82 + Acetonitrile 18 + Acetic Acid 1 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 285 nm


γ-Oryzanol Standard, 98.0+% (molecular absorptiometric analysis)

156-02831

Mixture of steryl ferulates

CAS No. 11042-64-1


Oryzae Semen 20 mg

Grade Storage


Example analysis

TLC Molecular absorptiometric analysis

Sample: 2.5 mg/mL (Ethyl Acetate)Sample spotted: 10 μLThin-layer plate: Silicagel 60 F254, 20 × 5 cmDeveloping solvent: Hexane 5 + Acetone 2 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV 365 nm

Sample: 10μg/mL (Heptane 1))Detection method: Measure the absorption maximum wavelength around 315 nm.

1) for HPLC


Oxymatrine Standard, 98.0+% (HPLC) 150-01511

C15H24N2O2 = 264.37CAS No. 16837-52-8


Sophorae Radix 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Chloroform1 + Methanol 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT.→Dry at 80℃ for 10 min.→Allow to cool.→Spray

Dragendorff's Reagent 1)→Visual judgment.

1) Bismuth Nitrate Basic 0.17 g + Acetic Acid 2 mL + Water 8 mL→ Shake + Potassium Iodide Solution (400 g/L) 10 mL→ Shake.


[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 35℃Eluent: [0.02 mol/L Phosphate buffer 1) 65 + Acetonitrile 35 (volume ratio)]400 mL + Sodium Dodecyl Sulfate 0.2 gFlow rate: 1.0 mL/minDetector: UV 220 nm

1) Potassium Dihydrogen Phosphate 0.81g + Water (→300 mL) → Adjust the pH to 2.5 with Phosphoric Acid.


37

Cru

de

Dru

g Te


Plantago Seed 166-23751

Unidentified


Plantaginis Semen 10 g

Grade Storage


Example analysis

TLC

Sample: Add the 2 g in 3 mL of Methanol and heat for 3 min. in a water bath. After cooling, centrifuge followed by taking the supernatant.　

Sample spotted: 10 μL Thin-layer plate: Silicagel plate for TLCDeveloping solvent: Acetone 10 + Ethyl Acetate 10 + Water 3 + Acetic Acid (100)

1 (volume ratio) or　Ethyl Acetate 6 + Water 1 + Formic acid 1 (volume ratio)

Distance spot travels: abt. 10 cmDetection method: After air drying, spray 4-methoxybenzaldehyde ・ Sulfuric Acid

reagent. → Heat at 105℃ for 10 min. → Visual judgment


Praeruptorin A Standard, 98.0+% (HPLC) 168-22231

C21H22O7 = 386.4CAS No. 73069-25-7



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol) Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Diethyl Ether 3 + Hexane 1 (volume ratio)Distance spot travels : abt. 10 cmDetection method: UV 365 nm

Sample: 1 mg/mL (Methanol) Injection: 5 μLColumn: Wakopak Navi C18-5, 4.6 mmφ × 15 cm

[Wako Cat. No.: 237-60513 (W),231-60511 (D)] *Column Temperature: 40℃Eluent: Water 45 + Acetonitrile 55 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 322 nm


Rhaponticin Standard, 98.0+% (HPLC) 189-02721

C21H24O9 = 420.41CAS No. 155-58-8


Rhei Rhizoma 5 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 2 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404] Devedloping Solvent: Ethyl Formate 10 + 2-Butanone 7 + Formic Acid 1

+ Water 1 (volume ratio) Distance spot travels: abt. 10 cmDetection method: UV exposure (dominant wavelength: 365 nm)

Sample: 0.2 mg/mL (Methanol)Sample spotted: 5 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃ Eluent: 0.1 vol% Phosphoric Acid 65 + Methanol 35 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 324 nm


38

Cru

de D

rug


Rutin Standard, 95.0+% (HPLC) 188-02791

C27H30O16 = 610.52CAS No. 153-18-4


Crataegi Fructus 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Methanol) Sample spotted: 2 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Ethyl Acetate 5 + 2-Butanone 3 + Water 1 + Formic Acid 1 (volume

ratio)Distance spot travels: abt. 10 cmDetection method: After air drying, spray diluted sulfuric acid.→After heating at 105℃

for 5 min, expose to UV (dominant wavelength: 365 nm)

Sample: 0.4 mg/mL (Methanol) Injection: 5 μLColumn: Wakosil 5C18,4.6 mm φ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 40℃Eluent: Water 70 + Methanol 30 + Acetic Acid 5 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254nm


Saikosaponin c Standard, 99.0+% (HPLC) 197-08421

C48H78O17 = 927.12CAS No. 20736-08-7



Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: The lower layer of [Chloroform 7 + Methanol 3

+ Water 1 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p-Anisaldehyde 1 g + Ethanol (99.5) 50 mL

+ Sulfuric Acid (1+5) 50 mL].→ Heat →Visual judgment.

Sample: 0.5 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG，4.6 mmφ × 15 cm



Scopolamine Hydrobromide n-Hydrate Standard, 99.0+% (HPLC)

190-16341

C17H21NO4・HBr = 384.26CAS No. 114-49-8


Scopoliae Rhizoma 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 0.6 mg/mL (Ethanol (99.5))Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Methanol 200 + 28% Ammonia Solution 3 (volume ratio)Distance spot travels : abt. 10 cmDetection method: Dry at RT.→Spray Dragendorff's Reagent 1) → Visual judgment.

1) Bismuth (III) Nitrate Basic 0.17g + Acetic Acid 2 mL+ Water 8 mL → Shake + Potassium Iodide Soln. (400 g/L) 10 mL→ Shake.


[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Phosphoric Acid・Triethylamine buffer 1) 9 + Acetonitrile 1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 210 nm

1) Potassium Dihydrogen Phosphate 6.8 g + Water 900 mL + Triethylamine 10 mL + Phosphoric Acid (→pH 3.5) + Water (→ 1 L).


39

Cru

de

Dru

g Te


Sennoside A Standard, 98.0+% (HPLC) 194-16241

C42H38O20 = 862.74CAS No. 81-27-6


・Sennae Folium・Rhei Rhizoma

10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Tetrahydrofuran 7 + Water 3 (volume ratio))Sample spotted: 20 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Ethyl Acetate 40 + 1-Propanol 40 + Water 30

+ Acetic Acid 1 (volume ratio)Distance spot travels:abt. 15 cmDetection method: UV 365 nm


[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃Eluent: [Acetic Acid･Sodium Acetate buffer (pH 5.0) 1) 17 + Acetonitrile 8 (volume ratio)] 1 L

+ Tetra-n-heptylammonium Bromide 2.45 gFlow rate: 0.7 mL/minDetector: UV 340 nm

1) [Sodium Acetate Trihydrate 13.6 g + Water ( → 100 mL)] ＋ [Acetic Acid 6 g + Water (→100 mL)](→pH 5.0) →Take the 100 mL + Water (→1 L).


Sennoside B Standard, 98.0+% (HPLC) 197-16091

C42H38O20 = 862.74CAS No. 128-57-4


・Sennae Folium・Rhei Rhizoma

10 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Tetrahydrofuran7 + Water 3 (volume ratio))Sample spotted: 20 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Ethyl Acetate 7 + 1-Propanol 7 + Water 4

+ Formic Acid 2 (volume ratio)Distance spot travels: abt. 15 cmDetection method: UV 365 nm


[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃Eluent: [Acetic Acid･Sodium Acetate buffer (pH 5.0) 1) 17 + Acetonitrile 8 (volume ratio)] 1 L

+ Tetra-n-heptylammonium Bromide 2.45 gFlow rate: 0.7 mL/minDetector: UV 340 nm

1) [Sodium Acetate Trihydrate 13.6 g + Water ( → 100 mL)] + [Acetic Acid 6 g + Water ( → 100 mL)](→ pH 5.0) →Take the 100 mL + Water (→ 1 L).


Shikonin (mixture of optical isomers, abt. 6 : 1), 98.0+% (HPLC)

198-13581

C16H16O5 = 288.30CAS No. 517-89-5



Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Acetone) Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Visual judgment and UV exposure

Sample: 1 mg/mL (Methanol) Injection: 5 μLColumn: CHIRALCEL OJ-H, 4.6 mmφ × 25 cmColumn Temperature: 40℃Eluent: Hexane 95 + 2-Propanol 5 + Acetic Acid 0.3 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 273 nm


40

Cru

de D

rug


Shikonin (mixture of optical isomers, abt. 1 : 1), 98.0+% (HPLC)

198-13601

C16H16O5 = 288.30CAS No. 54952-43-1



Grade Storage


Example analysis

TLC HPLC (1)

Sample: 5 mg/mL (Acetone)Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: ①Visual judgment　②UV exposure

Sample: 1 mg/mL (Methanol) Injection: 5 μLColumn: CHIRALCEL OJ-H, 4.6 mmφ × 25 cmColumn Temperature: 40℃Eluent: Hexane 95 + 2-Propanol 5 + Acetic Acid 0.3 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 273 nm

HPLC (2)

Sample: 1mg/mL (Methanol)Injection: 5 μLColumn: Wakosil-II 5C18 HG，4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 2 vol% Phosphoric Acid 25 + Methanol 75 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 273 nmQuantitation: Percentage by area


Shikonin Standard, 99.0+% (HPLC) 191-13331

C16H16O5 = 288.30CAS No. 517-89-5



Grade Storage


Example analysis

TLC HPLC

Sample: 5 mg/mL (Acetone)Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure and visual judgment

Sample: 1 mg/mL (Methanol)Injection: 5 μLColumn: Wakosil-II 5C18 HG，4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 2 vol% Phosphoric Acid solution 25 +Methanol 75 (volume ratio)Flow rate: 1.0 mL/min.Detector: UV 273nm


Sinomenine Standard, 98.0+% (HPLC) 193-09361

C19H23NO4 = 329.39CAS No. 115-53-7


Sinomeni Caulis et Rhizoma 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 10 mg/mL (Chloroform)Sample spotted: 2 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Chloroform 20 + Methanol 20

+ Ammonia Solution 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure

Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18，4.6 mmφ × 15 cm

[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room temperatureEluent: Sodium Dodecyl Sulfate Solution (5 g/L) 11 + Acetonitrile 9 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 260 nm


41

Cru

de

Dru

g Te


Swertiamarin Standard, 98.0+% (HPLC) 198-16901

C16H22O10 = 374.34CAS No. 17388-39-5


Swertia Herb 20mg

Grade Storage


Example analysis

HPLC

Sample: 2 mg/ 10 mL (Eluent)Injection: 10μLColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 10 + Water 90 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 238nm


Tauroursodeoxycholic Acid Standard, 98.0+% (HPLC)

203-17061

C26H45NO6S = 499.70CAS No. 14605-22-2


Fel Ursi 20mg

Grade Storage


Example analysis

TLC HPLC

Sample: 10 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 10 + Acetic Acid 10 + Water 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT.→Spray Sulfuric Acid (1+18) →Heat at 105℃ for 10 min.

→ Visual judgment.

Sample: 0.5 mg/mL (Methanol)Injection: 10 μL Column: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm


1) Potassium Dihydrogen Phosphate1.36 g + Water 450 mL + Phosphoric Acid (→pH 3.0) + Water (→500 mL).


Timosaponin A-III Standard, 98.0+% (HPLC)

207-16861

C39H64O13 = 740.92CAS No. 41059-79-4


Anemarrhenae Rhizoma 20 mg

Grade Storage


Example analysis

TLC HPLC

Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method : Dry at RT.→ Spray [Sulfuric Acid 1 + Ethanol (99.5) 20 (volume

ratio)]→ Dry at RT. → Heat at 105℃ for 5 min.→Visual judgment.

Sample: 10 mg/mL (DMSO 3 + Methanol 7)Sample spotted: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm

[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Water 50 + Acetonitrile 50 (volume ratio)Flow rate: 1.0 mL/minDetector: Differential refractometer detector


42

Product Name PAGE

Index

AAchyranthes Root ···························· 1, 20Aconitine Standard ······························ 20Aconitum Diester Alkaloids Standard ······· 1Aconitum Monoester Alkaloids Standard ····· 1Acteoside Standard ····························· 20Albiflorin ················································· 1Alisol A ··················································· 1Alisol B Acetate ··································· 21Alisol B Standard ································· 21Alkannin Standard ······························· 21Amygdalin ·············································· 2Arbutin ··················································· 3Arctiin Standard ··································· 22Artemisia Argyi ································ 3, 22Asarinin ·················································· 2(E )-Asarone ··········································· 2Astragaloside IV ···································· 2Atractylenolide III ··································· 2Atractylodin Standard ·························· 22Atractylodin TS ······································ 3Atropine Sulfate Hydrate ······················· 3Aucubin Standard ································ 23

BBaicalein Standard ······························ 23Baicalin ·················································· 3Barbaloin ··············································· 4Benz [a] anthracene ······························· 4Benzo [a] pyrene ···································· 4Benzoylmesaconine Hydrochloride ······· 4Berberine Chloride Hydrate ··················· 4Bergenin ················································ 5Bisdemethoxycurcumin ························· 5Bufalin ··················································· 5Bufotalin Standard ······························· 23

CCalycosin 7-O-Glucoside Standard ······· 24(E )-Capsaicin ········································· 5Carbazochrome Sodium Sulfonate Trihydrate ············································· 24Catalpol Standard ······························· 24Chikusetsusaponin IV ···························· 5(E )-Chlorogenic Acid ····························· 6(E )-Cinnamaldehyde ······························ 6(E )-Cinnamic Acid ································· 6Cinobufagin ··········································· 6Cinobufotalin Standard ······················· 25Coptisine Chloride ································· 6Corosolic Acid Standard ····················· 25Corydaline Standard ···························· 25Curcumin ··············································· 7Cycloartenyl Ferulate ····························· 7

DDehydrocorydaline Nitrate ····················· 7Dehydrocostus Lactone Standard ······· 26Demethoxycurcumin ····························· 7Dihydrocapsaicin Standard ················· 265,7-Dimethoxyflavone Standard ·········· 262,6-Dimethoxyphenol Standard ·········· 27Dimethylesculetin Standard ················ 27

EEchinacoside Standard ······················· 27

Eleutheroside B ····································· 7Emetine Dihydrochloride Standard ····· 28Epihesperidin Standard ······················· 28Ergosterol Standard ····························· 28β-Eudesmol Standard ························· 29Evodiamine Standard ·························· 29

F(E )-Ferulic Acid ······································ 8Fructose ················································ 8

GGeniposide ············································ 8Geniposidic Acid Standard ·················· 29Gentiopicroside ····································· 8[6]-Gingerol ············································ 8[6]-Gingerol Standard ·························· 30[8]-Gingerol Standard ·························· 30[10]-Gingerol Standard ························ 30Ginsenoside Re ····································· 9Ginsenoside-Rb1 Standard ················· 31Ginsenoside Rc ····································· 9Ginsenoside-Rd Standard ··················· 31Ginsenoside-Rg1 ··································· 9Glabridin ·············································· 314'-O-Glucosyl-5-O-methylvisamminol ····· 9Glycocholic Acid Standard ·················· 32Glycohyodeoxycholic Acid Standard ····· 32Glycyrrhizic Acid ···································· 9Glycyrrhizic Acid Standard ·················· 32Gomisin A Standard ···························· 33Gomisin N Standard ···························· 33Guaiacol ·············································· 10

HHesperidin ··········································· 10Hirsutine ·············································· 10Honokiol ·············································· 1010-Hydroxy-2-(E )-decenoic Acid ········ 103-(3-Hydroxy-4-methoxyphenyl)-2-(E )-propenoic Acid ···································· 11Hyodeoxycholic Acid ··························· 11Hypaconitine ······································· 33Hyperoside ·········································· 11

IIcariin ··················································· 11Isoacteoside Standard ························ 34Isofraxidine Standard ·························· 34

L(Z)-Ligustilide Standard Solution ········· 34Limonin ················································ 11Liquiritin ··············································· 12Loganin ················································ 12Luteolin ················································ 12

MMagnoflorine Iodide Standard ············· 35Magnoflorine Standard ························ 35Magnolol ·············································· 12Manninotriose ······································ 12l-Menthol ············································· 13Mesaconitine ······································· 35(E )-2-Methoxycinnamaldehyde ··········· 13Myristicin ············································· 13

NNaringin ··············································· 13Neohesperidin Standard ····················· 36Nodakenin ··········································· 13

Oγ-Oryzanol Standard ··························· 36Osthole ················································ 14Oxymatrine Standard ·························· 36

PPaeoniflorin ·········································· 14Paeonol ··············································· 14Palmatine Chloride ······························ 14Perillaldehyde ······································ 14Plantago Seed ······························· 15, 37Powdered Swine Bile ·························· 15(±)-Praeruptorin A ······························· 15Praeruptorin A Standard ······················ 37Puerarin ··············································· 15

RResibufogenin ····································· 15Rhaponticin Standard ·························· 37Rhein ··················································· 16Rhynchophylline ·································· 16Rosmarinic Acid ·································· 16Rutin Standard ···································· 38

SSaikosaponin a ···································· 16Saikosaponin b2 ··································· 16Saikosaponin c Standard ···················· 38Saikosaponin d ···································· 17Schizandrin ·········································· 17Scopolamine Hydrobromide n-Hydrate Standard ·············································· 38Scopoletin ··········································· 17Sennoside A Standard ························ 39Sennoside B Standard ························ 39Sesamin ··············································· 17Shikonin ········································· 39, 40Shikonin Standard ······························· 40[6]-Shogaol ·········································· 17Sinomenine Standard ·························· 40Sodium Glycocholate ·························· 18Sodium Tauroursodeoxycholate ·········· 18Stachyose ············································ 18Swertiamarin ······································· 18Swertiamarin Standard ························ 41

TTauroursodeoxycholic Acid Standard ····· 41Theophylline ········································ 18Timosaponin A-III Standard ················· 41

UUmbelliferone ······································ 19

VVerbascoside ······································· 20

WWogonin ·············································· 19

IGZ Instruments AGRäf felstrasse 32CH – 8045 Zürich

Tel. +41 44 456 33 33Fax +41 44 456 33 30www.igz.ch [email protected]

Instrumentssmart solutions & service

jp crude drug test - igz.ch drug analysis catalogue low res.pdf · capsici fructus 20 mg grade...

Documents