ista forest tree and shrub seed committee 2010 - 2011

ISTAForest Tree and Shrub Seed

Committee2010 - 2013

ISTA Annual Meeting, Venlo NLJune 12, 2012

Chair Fabio Gorian Italy GER, BSC, RUL

Vice-chair Zdenka Prochazkova Czech Republic

Members Isolde D. KossmannFerraz

Brazil

Robert P. Karrfalt United States AOSA, MOI

Dave Kolotelo Canada

Stefanie Krämer Germany TEZ

Beti Piotto Italy

Hugh W. Pritchard United Kingdom STO

Heidi Røsok Bye Norway

Dale Simpson Canada

Victor Vankus United States AOSA

Rosa Colomer Spain

Efisio Mattana Italy

Czeslaw Koziol Poland

Vacant

FTSC MEMBERS

A- Rules developmentA1 Introduction of new methods

1.Water activity measurement

2.Alternative germination substrate for conservation programs and small lots

3.MOI: working sample


1. Alternative method to the moisture content determination2. MOI and AW were compared in 2012 in ITML 0600

Water activity measurement


The use of the agar for germination tests in conservation programs and with smalllots

1. sterile substrate;2. Homogeneous and constant moisture conditions

Proposal to Germination Committee and Storage


Discussion is open among MOI members to change ISTA rule n° 9.1.5.2 (and 9.1.5.5):

Proposal: also for large seeds (as tree seeds) 2 working samples each of 5 gr

MOI cutting working group

Scope 1: to verify if cutting is compulsory for the seeds of Pinus genus when the TSW is > 200 g.

Scope 2: to verify if it is possible to test 2 working samples of 5 g (or 10 g) of seeds instead of 5 seeds x 2 replicates.

1. ITML0600

2. CZDL0200

3. USDL0300

4. DEDL0400

5. FRDL0200

6. BEDL0200

Partecipating labs

Samples are in preparation for distribution

MOI cutting working group

A- Rules developmentA2 Introduction of new species

1.Validation of germination methods for tropical/subtropical species2.Tetrazolium 3.Pretreatments on Mediterranean shrub seeds4.Juglans spp.

Basic germination protocols for many tropical tree species are available.

Palm seed germination at 30°C

0

5

10

15

20

<20 <40 <60 <80

Mean time to germinate (d)

Num

ber o

f spe

cies


34 Palm species (Pritchard, Mark, 2011)

Rubia peregrina subsp. peregrina Rubus ulmifolius

Species 1000 TSW TEZ test MOI behaviour

Rubia peregrina L. subsp. peregrina 14,5 g 95% Orthodox

Rubus ulmifolius Schott 2,6 g 93% Orthodox

ITML 0600



GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES*(May 2012)

*Montaraz laboratories -Madrid (Spain)

SPECIES TREATMENT % GER DAYS

Arbutus unedo Soaking in water (48 hours) + Cold Stratificacion (1 month). Tª (15-20ºC) 65-80 28-35

Cistus clusii Bowling water (1 minute) 45-60 25-30

Cistus creticus Soaking in water (24 hours) 60-70 35-40

Cistus laurifolia Bowling water (5 seconds). Tª(15-20ºC) 40-50 25-30

Colutea arborescens Bowling water (5 seconds) + Soaking in water (24 hours). Tª(15/25ºC) 70-85 35-45

Cytisus multiflorus Bowling water (5 seconds). Tª(15) 80-90 28-35

Genista scorpius Bowling water (5 seconds) + Ice + Soaking in water (24 hours) . Tª(15-20ºC) 60-70 28-35


GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES*(May 2012)

*Montaraz laboratories -Madrid (Spain)

SPECIES TREATMENT % GER DAYS

Halimium atriplicifolium Bowling water (5 seconds). Tª(15-20ºC) 60-70 25-30

Pinus halepensis No soaking before sowing 85-95 14-21

Pinus nigra laricio No soaking before sowing 85-95 14-21

Pinus nigra salzmannii No soaking before sowing 85-95 14-21

Retama monosperma SA(3 h)+CA(24 h) 70-80 14-21

1. Both ISTA and AOSA no rules2. Important commercial species

Only among European laboratories

ViableNo viable


Juglans spp.

B- PublicationsB1 Rules accompanying publications

1.Tree and shrub seed handbook (revision) No progress2.Sampling handbook (tree seed) Discussion is in progress

among BSC members about chapters 2,4,5 and 6

1.Guia de propagulos e plantulas da Amazonia, vol.2

2.Handbook on seedling evaluation(tree seed)

B- PublicationsB2 Training publications on specific seed testing topics

Fruits, Seeds and Seedlings of Amazonian Trees Vol. 2Ferraz, I.D.K., Camargo J.L.C. & Albuquerque M.C.F.

0123456789

10

Núm

ber o

f spe

cies

<10g 11-100g 101-1.000g

1.001-10.000g

10.001-100.000g

Thousand Seed Weight

0

1

2

3

4

5

Num

ber o

f spe

cies

0-1w 1-2w 2-3w 3-4w 1-2m 2-3m 3-4m 5-6m 7-8m 8-9m 9-10m 11-12m 1-2y

Mean Germination Time

Mean germination time: from 6 to 728 days

Seed size TSW varied between

3 g and 26,700 kg25 of 75 species concluded

25 species – Vol II

Species Fam. Species Fam

Duguetia chrysea Anno Bombacopsis nervosa Malv

Ephedranthus amazonicus Anno Carapa guianensis Meli

Xylopia amazonica Anno Carapa procera Meli

Couma guianensis Apoc Cedrela odorata Meli

Protium klugii Burs Brosimum parinarioides Mora

Protium peruvianum Burs Maquira sclerophyla Mora

Goupia glabra Cela Trymatococcus amazonicus Mora

Glycydendron amazonicum Euph Compsoneura ulei Myri

Abarema cochleata Faba Osteophloeum platyspermum Myri

Cedrelinga cataeniformis Faba Lacunaria jenmanii Quii

Eperua duckeana Faba Matayba arborescens Sapi

Eschweilera amazoniciformis Lecy

Eschweilera pseudodecolorans Lecy

Gustavia elliptica Lecy

Protium peruvianum Burseraceae

Osteophloeum platyspermum Myristicaceae

ISTA Annual Meeting, ZürichJune 14, 2011

1. Guia de propagulos e plantulas da Amazonia, vol.1 (English version)

2. Guia de propagulos e plantulas da Amazonia, vol.2

3. Handbook on seedling evaluation(tree seed)Ex. Adenocarpuscomplicatus

NormalAbnormal

The handbook “Seedlingevaluation” needs some standardization regarding the pictures…

1) Seed storage in polyethylene bags of a recalcitrant species (Quercus ilex): analysis of some bio-energetic and oxidative parametersS. Pasquini, M. Mizzau, E. Petrussa, E. Braidot, S. Patui, F. Gorian, M. Lambardi, A. Vianello

B- PublicationsB3 Scientific information publications

2) Interchangeable effects of giberellic acid and temperature on embryo growth, seed germination and epycotyl emergence in Ribes multiflorum ssp. sandalioticum(Grossulariaceae)E. Mattana, H.W. Pritchard, M. Porceddu, W.H. Stuppy, G. Bacchetta

B- PublicationsB3 Scientific information publications

1.Madrid (E): Workshop on Mediterranean species, June 20132.Milkow (PL): Workshop Current technologies of forest seed treatment, May 2012

C- Workshops and seminarsC1 Training and education workshops

Madrid Workshop, June 2013

Trees and shrubs seeds from the Mediterranean Basin: development of test methods to be

introduced into the ISTA Rules.

SEMILLAS MONTARAZ,

A SEED COMPANY WITH A QUALITY SEED LABORATORY

SPECIALIST OF TREES, SHRUBS, WILDS, HERBS AND NATIVE SEEDS SPECIES FROM THE MEDITERRANEAN AREA

SINCE 1990 – MADRID - SPAIN

QUALITY SEED LABORATORY

Skilled professionals

Personal member of TCM/FTS

Equipment and

protocols following

ISTA procedures

R&D on methods and treatments

Quality control, improvement, treatments and conservation of seeds from Mediterranean and Sub-tropical plant species.

850 Mediterranean and sub-tropical plant species of seeds from 1,800 plant species genotypes and varieties from 800 genera Mediterranean and worldwide.

Joint Research & Development programmes with universities and research centres on seed treatments and adaptation to environmental changes.

QUALITY SEED LABORATORY

Plant Biology DepartmentEscuela Universitaria de Ingeniería Técnica Agrícola

Technical University of Madrid• Located near de city center with good public transport (underground)• Conference room and laboratories

Researchlaboratory

Teachinglaboratory

Seminar room

The team

• Seed germination and conservation research group:– Félix Pérez-García (Professor)– M. Elena González-Benito (Professor)– Sara Mira (lecturer)

• Over 25 years experience on seed germination and conservation research work mainly with Mediterranean species

Seed germination of Phillyrea angustifolia

• Phillyrea angustifolia

• Oleaceae, related to Ligustrum and Olea

• A representative evergreen Mediterranean shrub

• Distribution: Northern Africa and South of Europe

• Economic importance: ornamental

• Fruit: black-blue drupe with lignified endocarp

• Seed oil content: 21%

•

Mira S., Ruiz C., González-Benito M.E. and Pérez-García F.

Departamento de Biología Vegetal, E.U.I.T. Agrícola, Universidad Politécnica de Madrid (UPM),Madrid, Spain

CONCLUSIONS:

• Germination needs the complete removal of the endocarp

• Germination is higher at 15-20ºC

• Seed germinations is faster at 15ºC

• Water imbibition for 72h increases the speed of germination

• Cold stratification has a negative effect on seed germination

• Plant growth in greenhouse is very slow

Seed germination of Phillyrea angustifolia

WORKSHOPCURRENT TEHNOLOGY OF FOREST SEED TREATMENT

21-25 MAY 2012, MILKOW, POLANDKostrzyca Forest Genebank

WORKSHOPMiłków, Poland

The aims of the workshop were to provide information on:

- the use of Polish forestry practices and treatment of seed material, - dissemination of actions, to encourage workshop participants to include set standards for work procedures used in their business units,- creating the possibility of an international exchange of experience

The workshop participants came from 11 European countries and they represented

the following institutions: State Forests

National ParksUniversities and Research Institutes

ISTAENSCONETMSB Kew

FAO


The main goals of the workshop

1. Report from the FAO Forest Genetic Resources Working Group - the status of forests / forest conservation in Europe.

2. Collection of seeds of forest trees and shrubs, herbaceous plants and crops - a comparison of accepted standards.

3. Seed quality testing and methods of storage:storage methods (length and conditions) depending on the category of seeds (orthodox, suborthodox, recalcitrant).Creation of seed samples.

4. Stratification and other dormancy breaking methods: stratification in the substrate and without substrate; length and conditions of stratification; other dormancy breaking methods


The main conclusions of the workshop

1.Workshop participants exchanged their knowledge and their experience in topics related to collection, evaluation and storage of herbaceous plants, crops and forest seeds. 2.Emphasized the need to organize the next workshop in another seed bank.3.Emphasized the necessity of extend educational activities for private forest owners relating to the purchase, handling and planting of recommended forest reproductive material in the context of ongoing climate changes


Paris, April 24-25, 2012OECD-Technical Working Group on forest scheme

1.Guidelines on the FRM chain2.Short rotation plantation for bioenergy and agro-forestry and shrub species3.Proposal to introduce the category “Tested” in the foreste scheme4.Uganda new member (26th)

Thank you for your attention!

ista forest tree and shrub seed committee 2010 - 2011

Documents