Ion 16S™ Metagenomics Kitand Ion Reporter™ metagenomics workfl ow solutionCulture-free rapid identifi cation of polybacterialresearch samples using the Ion PGM™ System

Ion 16S™ Metagenomics KitMetagenomics research is made easier with next-generation sequencing. With this culture-free sequencing method, you can get genus- and species-level taxonomic results in less than 3 days. The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. The amplifi ed fragments can then be sequenced on the Ion PGM™ System and analyzed using the Ion 16S™ metagenomics workfl ow in Ion Reporter™ Software (Figure 1).

The combination of the two primer pools enables broad-range, sequence-based identifi cation of bacteria from complex mixed populations.

ANALYZESEQUENCESAMPLE

ION PGM™ SEQUENCER

ION REPORTER™

SOFTWAREION 16S™ METAGENOMICS KIT

Tube 1: V2, V4, V8Tube 2: V3, V6–7, V9

My Sample Tube 2Tube 1

Figure 1. Integrated, cost-effective, scalable 16S rRNA sequencing workfl ow for the Ion PGM™ System, with a rapid workfl ow from library to analyzed results. Following library construction and template preparation, 400 bp sequencing runs are completed in just 2.3 hours for the Ion 314™ Chip v2 and 4.4 hours for the Ion 318™ Chip v2. BAM fi les produced with Torrent Suite™ Software are automatically analyzed, annotated, and taxonomically assigned in the Ion Reporter™ Software 16S metagenomics workfl ow.

Benefi ts of the Ion 16S™ Metagenomics Kit include:• Use starting material from direct

samples without culture, mixed culture, or pure culture

• Comprehensive primer setsallow for accurate detection andidentifi cation of a broad rangeof bacteria, down to genusor species level

• Flexibility—primer sets provided as two pools allow for separate analyses using individual pools

• Primers can also be barcoded

• Integrated workfl ow/end-to-end solution

Try the workflow at lifetechnologies.com/ion16smetagenomicsFor Research Use Only. Not for use in diagnostic procedures. © 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CO119380 0914

Ion Reporter™ Software— beta metagenomics workflowIon Reporter™ Software enables the rapid identification (at genus or species level) of microbes present in complex polybacterial research samples, using both curated Greengenes and premium curated MicroSEQ® ID 16S rRNA reference databases. The Ion Reporter™ metagenomics workflow also provides primer information, classification information, percent ID, and mapping information. It’s easy to interpret population diversity for your research at any taxonomic level with the interactive display (Figure 2).

Benefits• Premium curated MicroSEQ® ID

and curated Greengenes databases

• Fast analysis with easy interactive Krona chart result display

• Try it at lifetechnologies.com/ionreporter Figure 2. Automated analysis, annotation, and taxonomical assignment implemented in

Ion Reporter™ Software. Consensus view at the genus level of the balanced mock bacterial sample, using more stringent analysis parameters. Slash calls at the genus level are circled in red.

Ordering information

Product Cat. No.

Ion 16S™ Metagenomics Kit, 100 reactions A26216