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ANIMAL ETHICS - ONLINE APPLICATION QUESTIONS

This document allows you to review the questions that are part of the animal ethics application process, the application variation request process as well as the various reports to assist with your preparation of responses.

The Animal Ethics application, application variation request and reports in RIMS have six tabs, one of which includes a questionnaire which must be completed by applicants. To facilitate researcher planning, this document provides the questions applicants will encounter within the Questionnaire Tab when lodging an animal ethics submission in RIMS.

The questions are ordered as they will appear in RIMS. However, not all applicants will see all questions as the nature of the questions presented is dependent on the responses provided to previous questions.

All applicants submitting an animal ethics application will be asked the questions in the Introduction section where the questionnaire will determine whether the users submitting an application that falls outside the scope of animal ethics approval. If the user’s application is automatically determined to be outside of scope, the questionnaire will then ask the applicant to please submit their application for record keeping purposes.

A range of reference and support materials, such as Quick Reference Guides to aid users in creating and submitting an animal ethics application, application variation request or report, are provided at the Office for Research Animal Ethics website: (https://www.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications).

Introduction..........................................................................................................................................2

Scope Assessment.............................................................................................................................3

Application Type Selection................................................................................................................5

Laboratory Research Questionnaire Branch...................................................................................6

Field Research Questionnaire Branch...........................................................................................16

Prior Reviewed Research Questionnaire Branch.........................................................................33

Application Variation Request.........................................................................................................35

Annual/Final/Progress Report Questionnaire Branch..................................................................37

Adverse Event Report Questionnaire Branch...............................................................................41

Office for Research 08 March 2019

https://www.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications


IntroductionWelcome to Griffith University's Animal Ethics Application Questionnaire. In this questionnaire, you will be guided through a series of questions to allow the Animal Ethics Committee to assess your application.

Please note that there is a limit of 15 minutes to complete each question. If this time is exceeded your answer may not be saved by the system. It is recommended that you prepare long answers outside of RIMS and paste your responses into the questionnaire.

If you choose to edit your response to a previous question, please note that your responses to subsequent questions will be deleted. The restore button can be used to bring back your subsequent answers if this occurs, however only your first answer submitted for each question will be preserved. To ensure that your most recent responses are available to you, use the 'print form' button on the coversheet tab to produce a word document containing all information entered into the application. Save this file as a reference each time you change your responses.

For further information regarding application submissions, please consult the animal ethics website or contact the ethics team. If you experience any technical difficulties, please contact the INS RIMS support team at: [email protected] or 07 373 55444.

Continue – Go to the next introduction statement

Before completing the Animal Ethics Questionnaire please refer to the Australian code for the care and use of animals for scientific purposes (Code) and all other Acts and Guidelines applicable to your proposed research, such as: Animal Care and Protection Act (2001) (QLD)

The University is registered to use live animals for scientific purposes including teaching. If a project is conducted by Griffith University staff or students at another institution independent approvals from each AEC must be obtained. AEC approved Standard Operating Procedures are available here

Continue – Go to the next introduction statement

Please note, effective alternatives to using live animals must be considered. All use of animals for any purpose must be justified. Investigators have personal responsibility for all matters related to the welfare of the animals they use and must act in accordance with all requirements of the Code. This responsibility begins when an animal is allocated to a project and ends with its fate at the completion of the project. Investigators have an obligation to treat animals with respect and to consider their welfare when planning and conducting projects. Annual reporting is a requirement of the Code.

Proceed to questionnaire – Go to the scope assessment section


https://www.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications/standard-operating-procedures

https://www.legislation.qld.gov.au/LEGISLTN/CURRENT/A/AnimalCaPrA01.pdf


https://nhmrc.gov.au/research-policy/ethics/animal-ethics

https://www.nhmrc.gov.au/about-us/publications/australian-code-care-and-use-animals-scientific-purposes



Scope Assessment1- Does the proposed activity only include the use of animal tissues from a dead animal (e.g. cadavers, tissue samples, bones, fluids, excreta) where the animal was not killed for the collection of these tissues?

Yes – The responses you provided indicate that this work does not require animal ethics approval. The questionnaire has now been completed.

After uploading a short summary of your project to the 'Documents' tab, please click on the 'Coversheet' Tab and click 'Submit'. This application is to be submitted for record keeping purposes only, no further advice will be provided to you. If you wish to request an exemption notification, please contact [email protected]

No – Go to the next scope assessment question

2- Does the proposed activity only include the use of animals that are not legally defined animals (e.g. flies or other insects for scientific research)?




3- Does the proposed activity only include work with animals conducted for a non-scientific purposes (e.g. wildlife displays at schools or livestock displays at agricultural shows, diagnosis by a veterinarian within routine veterinary practice)?

Please note, teaching is considered a 'scientific purpose'.




4- Does the proposed activity include no use of animals, with animals not being interfered with in any way (e.g. observing visually or photography, not including spotlighting, eg. Bird watching and whale watching from a public beach using the


naked eye or binoculars) and no abnormal disruption of habitat (e.g. walking, remaining or driving in places to which people typically have that type of access such as public or national parks, tracks, roadsides and farmland)?



No – Go to the next (application type selection) question


Application Type Selection5- Has this application been previously or simultaneously submitted to this or another ethics committee? If yes, please provide reasons for re-submission or simultaneous submission and the name of the Animal Ethics Committee(s).

Text answer – Go to question 5a

5a- What type of application is this? If multiple apply, please contact [email protected]

Experimental (lab based) (research or teaching) – Start the Laboratory Research Questionnaire Branch

Free ranging wildlife (research or teaching) – Start the Field Research Questionnaire Branch

Protocol already submitted to another AEC – Start the Prior Review Research Questionnaire Branch


mailto:[email protected]

mailto:[email protected]

Laboratory Research Questionnaire Branch6- Please select the primary purpose from the drop down box below:

Maintain & improve human/animal health & welfare – Go to question 6a

Achieve educational outcomes in science – Go to question 6a

Improve animal management or production – Go to question 6a

Understand human or animal biology – Go to question 6a

Understand/maintain/improve the natural env. – Go to question 6a

6a - If this project involves undergraduate students, please describe how it will be ensured that that students have the opportunity to discuss the ethical and social issues, and legal responsibilities involved in the care and use of animals for scientific purposes before the use of animals commences. Please also describe what steps will be taken if a student conscientiously objects to the activity.

The ANZCCART Ethical guidelines for students using animals or animal tissues for educational purposes (https://www.adelaide.edu.au/ANZCCART/docs/aust-ethical-guide2013.pdf) is a useful resource for students.

If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 7

7- Please select the social relevance or significance from the drop down box below:

Conservation/Environment – Go to question 8

Medical Science – Go to question 8

Veterinary Science – Go to question 8

Basic Biology – Go to question 8

Other (please specify) – Go to question 7a to specify

7a- Please specify social relevance or significance if 'Other' was specified, otherwise type 'N/A' and press next to skip this question.


8- Please select the primary subject from the drop down box below. If none apply, please select 'None of the above'.


https://www.adelaide.edu.au/ANZCCART/docs/aust-ethical-guide2013.pdf


Behaviour – Go to question 9

Cell Biology – Go to question 9

Biomaterials – Go to question 9

Molecular biology – Go to question 9

Pharmacology – Go to question 9

Biochemistry – Go to question 9

Clinical sciences – Go to question 9

Genetics/gene manipulation – Go to question 9

Nutrition – Go to question 9

None of the above (next page) – Go to question 8a.

8a- Please select the primary subject from the drop down box below. If none apply, please select 'Other' and press next.

Toxicology – Go to question 9

Drug development – Go to question 9

Immunology – Go to question 9

Neurobiology – Go to question 9

Physiology – Go to question 9

Other (please specify) – Go to question 8b

8b- Please specify primary subject.


9- Acronyms and glossary. It is important that all AEC members are provided with sufficient information to complete an assessment of the proposal. To allow this, ensure that where the use of scientific language is deemed unavoidable, it is supported by a suitable lay description or included in the below glossary of terms.


10- Please state the scientific or educational aims in lay terms, avoiding the use of technical language so that they can be understood by a person without a scientific background. Where a project fits in a wider-body of animal research, investigators must include an outline of how the project relates to an overall program of work (additional information may be uploaded as a separate document)


Include any essential reference citations here. Remember to upload all essential papers to the Documents tab for reading by the Committee.


11- Please describe the potential impact and benefits of the outcomes of this research. If this application relates to a teaching activity, justification must include an outline of how the attainment of educational outcomes will be assessed, including, as applicable, national educational outcomes, required Vocational Education and Training (VET) package competency achievements, endorsed program outcomes and other curriculum-related outcomes.


12- Please explain the balance of harms to benefits in this study to support the case of ethical acceptability of the proposed use of animals. Please address whether the potential effects on the wellbeing of the animals involved is justified by the potential benefits of the research. The Code defines well-being as the positive mental state of an animal, able to have positive experiences, express innate behaviours, and respond and cope with potentially adverse conditions. The Code also states that the wellbeing of animals must be considered in terms of the cumulative effects of an animal’s lifetime experience. It is therefore necessary to weigh up the positive as well as the negative experiences of all animals involved, based on their will to survive and thrive, and to consider all alternative actions which could be maximise well-being and avoid harm to sentient beings. To answer this question, please refer to the Governing Principles of the Code (1.1, 1.3, 1.5, 1.6, 1.7 1.8).


13- Please provide a clear description of the steps taken to consider and apply the 3Rs (Replacement, Refinement, Reduction - see clauses 1.18-1.30 of the Code). Please comment on any refinements applied to methods and procedures that will applied and what efforts have been made to ensure current best practice is applied.

14- Please provided details of what is being done by you or your University, and/or others within or outside of your field to replace the use of animals to achieve the stated aims. Provide details of the potential alternative actions that could replace the use of animals in all or part of the project and why these alternatives are not currently being used.


15- If requesting animal tissue or other biological material only, can tissue be obtained from euthanised animals used for other projects?

Yes – Go to question 15a

No – Go to question 16

N/A – Go to question 16

15a- In response to the question 'If requesting animal tissue or other biological material only, can tissue be obtained from euthanised animals used for other projects?' Provide that AEC Protocol number below (if known):



16- In 2017, the NHMRC released a guidance document regarding Best practice methodology in the use of animals for scientific purposes which complements the governing principals of the Australian Code for the Care and Use of Animals for Scientific Purposes (NHMRC, 2013). This guidance documentis endorsed by the Australian Research Council (ARC), the Commonwealth Scientific Industrial Research Organisation (CSIRO) and Universities Australia. As part of the University’s implementation of this Guidance all relevant matters highlighted in Sections 1 and 3 of the Guidance must be addressed by the applicant before the Animal Ethics Committee may make a judgement about the ethical acceptability of the proposed use of animals. We recommend that all researchers are familiar with this guidance document.The AEC encourages researchers to consult the 3Rs website for information on Experimental design and statistics: https://www.nc3rs.org.uk/experimental-designstatisticsThe 3Rs experimental design assistant (EDA) can be of assistance in answering the questions below.https://www.nc3rs.org.uk/experimental-design-assistant-edaThe following questions address section 3.2 (Quality of Experimental Statistics) of the Best practicemethodology in the use of animals for scientific purposes (NHMRC, 2017).

Text answer – Go to question 16.1

16.1 Will you be using a statistical analysis as part of the research?


16.2 Please list (in order of priority) the research questions that you wish to address using a statistical

analysis.


16.3 What will you be measuring in each experiment (outcome measure) to address each research question? Outcome measure refers to the parameters that you intend to measure quantitatively. Please indicate if they are direct measures from the experiment, or derived from experimental measures. If they are derived, please explain how this measure is calculated.


16.4 Please indicate the planned statistical analysis for each outcome measure (E.g. T test, ANOVA).


16.5 Are there independent variables to be included in your analysis? (E.g., sex, breed, treatment).Please list these, and any interactions between these variables that may be required in the analysis(E.g. Do you expect that the treatment response may vary between the sexes?).



16.6 Please explain the design and data collection for the analysis, including information about experimental groups, and interventions. Please define this clearly, pictorially would be ideal. Diagrams can be uploaded as supporting documents in the documents tab. The EDA online tool can help to build a stepwise visual representation of an experiment.


16.7 In order to ensure that the number of animals requested are minimal but sufficient to achieve statistically robust results (Code, Clauses 1.21-1.27), please justify your sample size. If using a Power analysis, please indicate which sample size calculation you are using. Please also include the source of the Power analysis (eg. Statistical software package or online tool). Please note, the committee would like to see all inputs, such as anticipated group means, and standard deviations. Please reference papers and research from which these values are elicited, and if the research is entirely new, please provide the rationale for your assumptions or data from a pilot study. Also, please note that ideally you should consider multiple likely scenarios. Consider providing a set of power curves under multiple likely scenarios which take into account the potential variability of your results.). Please upload a supporting document with your statistical justification in the documents tab.


17- Have, or will, any of the animals be re-used in other experiments? If so, please specify the AEC protocol number (if known) and justify their use in this project by discussing the benefits balanced against the adverse effects on these animals.


18- Does this application involve repetition of previous experiments? If so, please justify the repetition of these experiments.


19- Provide a justification for use of the selected species, strain or breed. Please also justify why either one or both sexes will be used).


20- Upload a document titled 'Protocol' to the Documents Tab that provides a detailed but concise description of experimental protocol(s) & how they relate to the aims of the project.

Refer to Clause 2.7.4 of the Code to ensure that you provide all the required information. Include and fully cite essential references.

Ensure this section includes:

Details (sequentially) of what happens to the animal(s) from the time you obtain them until the time the project is completed. A flow chart or sequence of events table may assist in making this information clear.

Identify all factors and procedures that may have an impact on an animal’s wellbeing.



This may include handling, husbandry, housing etc as well as specific experimental procedures (Refer to the CHECKLIST) to ensure all details have been considered).

Where applicable, include information on procedures that apply to breeding programs that are integral to a project such as the creation of a new line of animals, including genetically modified or cloned animals or that are integral to the maintenance of a line of animals in a facility.

Include a table showing the number of animals to be used in treatments (and controls).

Continue… – Go to question 21

21- Daily animal monitoring is a condition of your ethics approval and should be documented in a monitoring scoresheet. Additionally, a clinical welfare score sheet should be completed if any abnormalities are detected during daily monitoring, to define signs of pain and distress and where specific interventions will take place to relieve pain and distress and implement humane endpoints. Upload a document titled 'Animal Monitoring and Clinical Score Sheets' to the Documents Tab. You may use your own score sheet or a template can be found here: This template MUST be modified with the criteria relevant to your specific project and animal model. It is important that you consider the likely clinical signs for your animal model. Each team member should be clear about what the signs are and how they are to be scored, and what interventions must occur.


22- How long will individual animals be held and/or subjected to experimental manipulations?


23- Proposed analgesic (pain relief) (dose rate and regime).


24- Does this project involve experimental studies of un-anaesthetised animals which may cause pain or distress and where analgesia will not be used? If so, state and justify the end point for the experiment and indicate measures to be taken to minimise pain and distress.


25- How long will animals be held after they recover from experimental procedures?


26- What will happen to animals at the completion of the project? If the animals will be humanely killed, how will this be done?




https://www.griffith.edu.au/__data/assets/pdf_file/0034/996415/CHECKLIST-for-lab-based-and-free-ranging-studies.pdf

27- Will animals be humanely killed within sight of other unanaesthetised active animals? If so, please justify why this cannot be avoided. Please also specify if a number of animals will be humanely killed simultaneously.


28- Does this experiment pose any health risk to staff or other animals? If so, how will this health risk be minimised?


29- Will the Griffith University Animal Facilities Staff be responsible for the maintenance and cleaning of cages/ aquarium/enclosures?



30- If you intend to house/use animals in the Griffith Biosciences Resources Facilities, please specify the intended facility(ies) on the Animal Usage Tab's Locations Tab. You may click on the Animal Usage tab now, enter the details, then return back to the Questionnaire Tab. Your responses will not be lost. If you intend to house/use animals at another location. Please provide details below:


31- Name the source(s) of the animals


32- Will Biosciences Resources Facility Staff be requested to maintain a breeding colony for this project? Describe the details.

Text answer – Go to question 32a or 33

32a- Please specify the micro and macro environment required. Ensure to include the following information:- Housing (group, individual or not applicable)- Cage type (Conventional, IVC, Micro-isolator or not applicable)- Bedding (Normal, Special or not applicable)- Feeding (Normal, Special diet, Special regime or not applicable)- Watering (Normal, Supplemented, Special regime or not applicable)- Temperature (Ambient, Other [please specify])- Humidity (Ambient, Other [please specify])- Containment (Normal, SPF, not applicable)


33- Animal marking and genotyping methods - Ear clip + gel analgesic (AEC preferred).

Note: Include justification of methods in your protocol document.

Yes – Go to question 34

Other – Go to question 34

N/A – Go to question 34


34- What environmental enrichment will be provided for these animals?


35- Will Biosciences Resources Facility staff be requested to perform technical work on animals in addition to routine husbandry?



35a- You indicated staff will be requested to perform technical work on animals in addition to routine husbandry. Provide details.


36- Will Biosciences Resources Facility staff be requested to provide training in any techniques required? If so please describe:


37- Have the details provided regarding animal facilities been discussed with the Biosciences Resources Facility Manager?



38- If the care and management of animals does not accord with current best practice, please provide details and a justification as to why this is necessary.


39- Are any external investigators involved in this project? If so, please ensure they are listed on the Coversheet and upload a signed External Researchers Declaration to the Document's tab prior to the submission of this application.


40- Will facilities of another institution be used?



40a- Please describe how facilities of another institution will be used.


41- Do any actual or potential interests exist, including any financial interest or other relationship or affiliation that may affect the judgement and decisions regarding the well being of the animals involved? If please describe.


42- Please specify what funding source and grant number is associated with this protocol (if applicable).



43- Please access the Team Member Table at https://www.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications and upload the completed table and emergency contact details to the Documents Tab. You may click on the Documents Tab now, upload the document, then return back to the Questionnaire Tab. Your responses will not be lost.

Please also ensure to assign either the Chief Investigator or one of the Internal Investigators as the Responsible Investigator on the Coversheet Tab via one of the role drop down boxes. The Responsible Investigator is the person with ultimate responsibility for the care and use of animals in the project (see 2.4.5 of the Code).


44- Are any Genetically Modified Organisms involved?



44a- Please specify the University Biosafety Committee (UBC) reference number and upload the approval memo and approved application to the Documents Tab.

If the application has not been approved by the UBC please note this below.

You may click on the Documents Tab now, upload the documents, then return back to the Questionnaire Tab. Your responses will not be lost.

Text answer – Go to the question 48

45- Does this proposal raise any other ethical concerns? If so, please elaborate.


46- Does this project require a scientific research and educational purposes permit (ECOACCESS)?



46a- Provide your Scientific research and educational purposes (ECOACCESS) permit number and upload a copy to the Documents tab.


47- Does this proposed animal use require an Australian Government - Department of Agriculture– Biosecurity Import Conditions System (BICON) import permit?







47a- Please specify the Australian Government - Department of Agriculture – Biosecurity Import Conditions System (BICON) import permit number and upload a copy to the Documents tab.


48- Is a prohibited matter permit required? (The Biosecurity Act 2014 lists prohibited matter in Schedule 1 of the Act).



48a- Please specify the prohibited matter permit number and upload a copy to the Documents tab.


49- Will any Schedule 8 drugs be used for this project?



49a- S8 Licence details. Provide the licence number and name of holder and upload a copy to the Documents tab.


50- Are any of your permits/approvals still pending?


No – Go to next question

50a- You've indicated that you have permits/approvals still in the process of approval. Please indicate the permit/approval type and their expected approval date (if known).

Text answer – Go to next question

The Laboratory research questionnaire has been completed. Please ensure you complete the 'Animal Usage' tab, and attach any necessary permits/approvals in the 'Documents' tab before hitting Submit on the coversheet.


Continue… – Questionnaire has been completed

Questionnaire has been completed


Field Research Questionnaire Branch6- Please provide the names of any PhD, Masters, Honours or Undergraduate Students involved in this project.


6a - If this project involves undergraduate students, please describe how it will be ensured that that students have the opportunity to discuss the ethical and social issues, and legal responsibilities involved in the care and use of animals for scientific purposes before the use of animals commences. Please also describe what steps will be taken if a student conscientiously objects to the activity.

The ANZCCART Ethical guidelines for students using animals or animal tissues for educational purposes (https://www.adelaide.edu.au/ANZCCART/docs/aust-ethical-guide2013.pdf) is a useful resource for students.



7- Please select the primary purpose from the drop down box below:

Maintain and improve human or animal health – Go to question 8

Understanding of human or animal biology – Go to question 8

Achievement of education outcomes in science – Go to question 8

Improvement of animal management or production – Go to question 8

Environmental Study – Go to question 8

8- Please select the social relevance or significance from the drop down box below. If none apply, please select 'Other'.

Conservation/Environment – Go to question 9

Basic biology – Go to question 9

Veterinary Science – Go to question 9

Other (please specify on next question) – Go to question 8a

8a- Please specify the social relevance or significance.





9- Please select the primary subject from the drop down box below. If none apply, please select other and press next.

Behaviour – Go to question 10

Ecology – Go to question 10

Biomaterials – Go to question 10

Nutrition – Go to question 10

Other (please specify on next question) – Go to question 9a

9a- Please specify primary subject area.


10- Acronyms and glossary. It is important that all AEC members are provided with sufficient information to complete an assessment of the proposal. To allow this, ensure that where the use of scientific language is deemed unavoidable, it is supported by a suitable lay description or included in the below glossary of terms.


11- Please state the scientific or educational aims in lay terms, avoiding the use of technical language so that they can be understood by a person without a scientific background. Ensure to include definitions for any acronyms or scientific terms when used for the first time. Where a project fits in a wider-body of animal research, investigators are asked to include an outline of how the project relates to an overall program of work (additional information may be uploaded as a separate document). Please also specify if the study is hypothesis-testing or hypothesis-generating.

Include any essential reference citations here. Remember to attach copies of all essential papers for reading by the Committee to the Documents Tab. You may click on the Documents Tab now, upload the document, then return back to the Questionnaire Tab. Your responses will not be lost.


12- Please describe the potential impact and benefits of the outcomes of this research. If this application relates to a teaching activity, justification must include an outline of how the attainment of educational outcomes will be assessed, including, as applicable, national educational outcomes, required Vocational Education and Training (VET) package competency achievements, endorsed program outcomes and other curriculum-related outcomes.


13- Please provide details of why the use of animals is essential to achieve all the stated aims. Provide details of the potential alternatives that are available to replace the use of animals in all or part of the project and why these alternatives are not suitable.



14- Please provide information about the balance of harm to benefit in this study to support the case of ethical acceptability of the proposed use of animals. Please address whether the potential effects on the wellbeing of the animals involved is justified by the potential benefits of the research. The Code defines well-being as the positive mental state of an animal, able to have positive experiences, express innate behaviours, and respond and cope with potentially adverse conditions. The Code also states that the wellbeing of animals must be considered in terms of the cumulative effects of an animal’s lifetime experience. It is therefore necessary to weigh up the positive as well as the negative experiences of all animals involved, based on their will to survive and thrive, and to consider all alternative actions which could be maximise well-being and avoid harm to sentient beings. To answer this question, please refer to the Governing Principals of the Code (1.1, 1.3, 1.5, 1.6, 1.7 1.8).


15- If animals are being captured, why is it necessary to capture animals? What alternatives to capturing animals could be used? What precautions will be taken if lactating animals or animals with pouch young are captured? Note: If using alternatives such as spotlighting or remote cameras, please detail procedures. E.g. the power of spotlights; and duration of use; if camera sites are baited/unbaited; if cameras use flash or infrared.

Note: Under section 42 of the Land Protection (Pest and Stock Route Management) Act 2002 a person must not, without reasonable excuse, release a declared pest other than under a declared pest permit. Under section 109 of the National Parks and Wildlife Act, it is an offence to liberate, anywhere in New South Wales, any animal other than a homing pigeon or a captured animal which is native to New South Wales.



16- Justify the use of animals for obtaining information which benefits the Primary Purpose you identified earlier in the questionnaire. A justification for use of the selected species must also be provided.


17- If animals will be humanely killed, is there the opportunity to share tissues and other biological material?



18- Provide a justification for the numbers of each species sampled per site per annum including evidence that the numbers are minimal, but statistically robust to achieve the aims of the research. Where appropriate, information MUST be provided on:

a) Experimental design and statistical considerations.


b) For teaching projects, the ratio of students to animals, and the number of times that each animal will be used in each class, and/or handled per day and/or per week.


19- List all study personnel who are blinded, or not blinded below. For all people who are not blinded, please provide a short explanation of why not. Please note, the failure to use blinding when performing an intervention, and when assessing results is a common flaw in experimental design which may lead to bias.


20- Is randomisation being used as a method of experimental control? Please describe how and why or why not.


21- Please provide a clear description of the steps taken to consider and apply the 3Rs (Replacement, Reduction, Refinement) (See clauses 1.18-1.30 of the Code).


22- Detail (sequentially) what happens to the animal(s) from the time you obtain them until the time the project is completed. A flow chart or sequence of events table uploaded to the Documents tab may assist in making this information clear.

Please also provide a template of the Fieldwork Animal Monitoring Record. An example template can be found under 'OFF-CAMPUS (FIELD) ANIMAL USE' at https://www2.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications

Note: Ensure to include a detailed but concise description of experimental or survey protocol(s) and how they relate to the aims of your project. Attach all essential references for the Committee's reading. Please refer to Clause 2.7.4 of the Code to ensure that you provide all the required information.


23- Identify all factors and procedures that may have an impact on an animal's well-being. This may include handling, husbandry, housing etc as well as specific experimental procedures (Refer to the CHECKLIST to ensure all details have been considered). Include prior experience of methods used (e.g. for trapping or tagging).

Also, please specify if an attrition rate is expected. If a higher than expected rate of attrition occurs, ensure to notify the AEC via the submission of an Unexpected Adverse Event Report in RIMS.



https://www.griffith.edu.au/__data/assets/pdf_file/0034/996415/CHECKLIST-for-lab-based-and-free-ranging-studies.pdf


https://www2.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications

https://www2.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications

24- Describe each factor or procedure and detail how any adverse impact will be minimised. Details should include treatment substances, dose rates, routes of administration, anaesthetic and analgesic regimes etc if applicable. (Refer to the CHECKLIST to ensure all details have been considered).


25- Location - where will the research take place? Please name the closest town(s):


26- Will any field research be done outside of Queensland?



27- Will any research be done in NSW?

Note: You may require a scientific licence from the NSW National Parks and Wildlife Service.



27a- Please indicate the primary purpose for the work based in NSW:

Education – Go to question 27b

Stock breeding – Go to question 27b

Stock maintenance – Go to question 27b

Research: human or animal Biology – Go to question 27b

Research: human or animal health and welfare – Go to question 27b

Research: animal management or production – Go to question 27b

Research: environmental study – Go to question 27b

Production of biological products – Go to question 27b

Diagnostic procedures – Go to question 27b

Regulatory product testing – Go to question 27b


https://intranet.secure.griffith.edu.au/__data/assets/pdf_file/0029/48764/CHECKLIST-for-lab-based-and-free-ranging-studies.pdf

27b- Have any of the project investigators ever had an animal research authority or animal suppliers licence cancelled?


28- Will any research be done in Tasmania?

Note: You may also require a scientific permit for research involving the collection or disturbance of protected wildlife, and/or an interstate import/export permit of wildlife and wildlife products and/or a permit to take freshwater and marine fish.



28a- Please indicate the primary purpose for the work based in Tasmania:

Note: An Animal Research Licence is required to undertake research in Tasmania. Additionally, the DPIPWE's Biodiversity Conservation Branch, Wild Fisheries Management Branch and the Inland Fisheries Service have requirements for the use of Tasmanian wildlife including fish in research. Investigators of licensed institutions are required to obtain a "Scientific Permit" before any wildlife is used for research.

Understanding biology – Go to question 29

Health and welfare – Go to question 29

Management or production – Go to question 29

Education – Go to question 29

Environmental study – Go to question 29

29- Will any research be done in Victoria?



29a- Please indicate the primary purpose for the work based in Victoria:

Note: Organisations or individuals wanting to conduct Scientific Procedures with animals in Victoria must hold an authorising licence issued by the Head of the Victorian Department of Economic Development, Jobs, Transport and Resources (DEDJTR). Scientific Procedures may be authorised by a Scientific Procedures Premises Licence (SPPL) or a Scientific Procedures Fieldwork Licence (SPFL) depending on the type of animals used, as well as the type of location where the work will be conducted.


You may also require a Wildlife Research Permit from the Department of Environment and Primary Industries.

Understanding biology – Go to question 29b

Health and welfare – Go to question 29b

Management or production – Go to question 29b

Education – Go to question 29b

Environmental study – Go to question 29b

29b- Please select the most appropriate option to describe the benefit of the protocol:

Fundamental biology/physiology – Go to question 30

Diseases – Go to question 30

Environmental monitoring – Go to question 30

Domestic management/production – Go to question 30

Wildlife management – Go to question 30

Vertebrate pest management – Go to question 30

Production of biological products – Go to question 30

Xenotransplantation – Go to question 30

Development of techniques – Go to question 30

Teaching – Go to question 29c

29c- In the last question you indicated 'Teaching' to describe the benefit of the protocol.

Please now choose the most appropriate sub-option to describe the benefit of the protocol:

Education (demonstration) – Go to question 30

Teaching (student use) – Go to question 30

30- Will any research be done in Western Australia?

Note: A licence from the WA Department of Agriculture and Food to use animals for scientific purposes is required in this state. Additionally, you may require a Licence to Take Fauna for scientific purposes. Where proposed activities may have an impact on the value of a World Heritage property or other values regulated by the Commonwealth Environment Protection and Biodiversity Conservation Act 1999, you are encouraged to contact the Commonwealth Department of Sustainability, Environment, Water, Population and Communities.




30a- Please indicate the primary purpose for the work based in WA:

Education – Go to question 31

Stock breeding (GM) – Go to question 31

Stock maintenance – Go to question 31

Research: Biology – Go to question 31

Research: health and welfare – Go to question 31

Research: management or production – Go to question 31

Research: environmental study – Go to question 31

Product testing – Go to question 31

31- Will any research be done in the Northern Territory?

Please note: To carry out research in the Northern Territory a licence from the NT Animal Welfare Authority is required. Please contact the Ethics & Integrity team to assist you in your application. For wildlife research, a permit to undertake scientific research is required from the NT Parks and Wildlife Commission and permission from the land holder on which the research is conducted is required.

Please upload these documents in the documents tab. You may click on the documents tab now, upload the document, then return back to the questionnaire tab. Your responses will not be lost.



32- Will any research be done in South Australia?

Please note: There are special requirements for undertaking research in South Australia. These include: Approval by a South Australian Animal Ethics Committee. You also need to obtain a licence for carrying out teaching, research or experimentation involving animals. Wildlife researchers may also need to obtain permits to take carcasses, blood, eggs or tissue samples from protected animals outside of South Australia. Permits from PIRSA Fisheries Division may also be needed if studying native fish. Please note, Investigators (rather than institutions) are responsible for all annual reporting requirements.




33- Will any research be done in the ACT?

Please note: There are special requirements for undertaking research in the ACT. Please contact the Research Ethics and Integrity team if you would like to conduct research in the ACT.



34- Are any animals used in this protocol located overseas?



34a- Please name the country/countries where animals used in this protocol are located and describe how the proposed project can meet the principles of the Code, taking into account compliance with local requirements. Please upload any relevant documentation in the documents tab. You may click on the documents tab now, upload the document, then return back to the questionnaire tab. Your responses will not be lost.


35- Will the methods described in the Terrestrial Vertebrate Fauna Survey Guidelines for Queensland (available at: https://www.qld.gov.au/environment/plants-animals/biodiversity/vertebrate-survey/) be followed? If not, why not?

If work is to be conducted within NSW, will the methods described in the Animal Research Review Panel's guideline 'Wildlife surveys' (available at: http://www.animalethics.org.au/policies-and-guidelines/wildlife-research/wildlife-surveys) and the Trapping Policy (available at: http://www.animalethics.org.au/policies-and-guidelines/wildlife-research/pitfall-traps) be followed to minimise impact on animals? If not, why not?


35a- How many traps will be set and over what period of time?

Text answer – Go to question 35b

35b- State the maximum number of traps per investigator that will be set. If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 35c


http://www.animalethics.org.au/policies-and-guidelines/wildlife-research/pitfall-traps

http://www.animalethics.org.au/policies-and-guidelines/wildlife-research/wildlife-surveys

http://www.animalethics.org.au/policies-and-guidelines/wildlife-research/wildlife-surveys

https://www.qld.gov.au/environment/plants-animals/biodiversity/vertebrate-survey/

35c- What traps* will be used?

*Note: If pitfall traps are to be used, the guideline 'Use of Pitfall Traps' by NSW Animal Research Review Panel available at: http://www.animalethics.org.au/policies-and-guidelines/wildlife-research/pitfall-traps must be followed.

*Note: If pitfall traps are to be used in QLD, the Terrestrial vertebrate fauna survey guidelines available at: https://www.qld.gov.au/environment/plants-animals/biodiversity/vertebrate-survey/ must be followed.

Text answer – Go to question 35d

35d- If using Mist Nets please detail experience and training in use. If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 35e

35e- How often and at what times will traps be checked and/or cleared? If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 35f

35f- Describe any other methods to be used for capture. If this question does not apply, please enter 'N/A' in the response box.


36- What precautions will be taken in the event of inclement weather?


37- Will samples be taken, e.g. milk, hair, scales? If so, how will samples be taken?


38- How will animals be handled or restrained?

Note: If capturing and handling amphibians compliance with the Threatened Species Management Information Circular No 6 - Hygiene protocol for the control of disease in frogs is required. Guideline is available at http://www.environment.nsw.gov.au/resources/nature/hyprfrog.pdf.

Note: If capturing and handling amphibians in NSW compliance with the Threatened Species Management Information Circular No 6 - Hygiene protocol for the control of


http://www.environment.nsw.gov.au/resources/nature/hyprfrog.pdf

https://www.qld.gov.au/environment/plants-animals/biodiversity/vertebrate-survey/



disease in frogs is required. Guideline is available at: http://www.environment.nsw.gov.au/resources/nature/hyprfrog.pdf.



39- If animals will be transported (such as to a base camp for holding or to a veterinarian for treatment), please provide details of transport including precautions against cold/heat stress?



40- Will animals be marked for identification? If so, how will they be marked?


41- Please identify known and potential causes of adverse impacts on the wellbeing of an animal and how such impacts will be avoided or minimised. Experimental and non-experimental factors must be addressed.


42- If invasive methods are to be used, how will pain/distress be minimised?


43- Will any radio tracking collars or other radio tracking equipment be used? If so, please specify what equipment will be used on the animal, how will it be attached, what is the weight of the equipment and the impact on the animal? How will the equipment be retrieved?


44- How long will individual animals be held and/or subjected to experimental manipulations? If this question does not apply, please enter 'N/A' in the response box.


44a- How long will animals be held after they recover from experimental procedures? If this question does not apply, please enter 'N/A' in the response box.


45- Is this a repetition of a previous experiment? If so, please justify the repetition of this experiment.



http://www.environment.nsw.gov.au/resources/nature/hyprfrog.pdf

46- Have, or will, any of the animals be used in other experiments? If so, please provide the AEC protocol number (if known) and justify their use in this project.


47- Does this project involve experimental studies of un-aneasthetised animals which may cause pain or distress and where analgesia will not be used. If so, please state and justify the end point for the experiment and indicate measures to be taken to minimise pain and distress.


48- Does this experiment pose any health risk to staff or to other animals? If so, how will this health risk be minimised?


49- Does this proposal raise any other ethical concerns? If so, please elaborate. (For example, what action would be taken if you encounter injured wildlife?)


50- What will happen to animals at the completion of the project?


51- Will voucher specimens be taken? If so, justify the taking and the number of voucher specimens and detail where the voucher specimens will be lodged.


52- How will you humanely kill animals if there is an emergency or for collection of voucher specimens? Please detail the procedures to be followed, specify where the humane killing will occur, by whom and detail their experience in the technique(s) to be used?


53- How will the animals be monitored during capture, handling, any other procedures and post release? Include frequency and methods used.


53a- Who will monitor the animals during capture, handling and post release? Include names, qualifications and experience with the species being used. Ensure to include


details for the monitoring during weekdays, weekends and holidays and at night (if applicable).


53b- Will animals be held even for a short time e.g. during the day prior to release? If so, justify why animals need to be held and not released immediately. Also include details of the locations where animals will be held and whether any procedure will be performed.


53c- How will the animals be held (including whether held separately or not) and in what type of facilities? If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 53d

53d- State dimensions of cages/pens. If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 53e

53e- What will be the duration of holding? If this question does not apply, please enter 'N/A' in the response box.

Text answer – Go to question 53f

53f- Will animals be fed? If so, what will they be fed and how often will they be fed?

Text answer – Go to question 53g

53g- Will the Griffith University Biosciences Resources Facility Staff be responsible for the maintenance and cleaning of cages/ aquarium/enclosures?

Yes – Go to question 53i

No – Go to question 53h

53h- If applicable, please attach a document to the Documents Tab titled 'Maintenance and Cleaning' specifying what maintenance and cleaning of cages/aquarium/enclosures will take place. Ensure to provide the schedule and specify who is responsible for each task described. For aquariums, ensure to describe the monitoring of water temperature and quality. You may click on the Documents tab now, upload the document, then return back to the questionnaire tab. Your responses will not be lost.

Proceed to next question – Go to question 53i


53i- What environmental enrichment will be provided for these animals?

Text answer – Go to question 53j

53j- If the care and management of animals does not accord with current best practice, please provide details and a justification as to why this is necessary.


54- Identify possible emergencies which might arise. E.g. animal injured in a trap, bushfire, hailstorm.


54a- List procedures you have in place to deal with these emergencies.


54b- In the event of an emergency involving animals used in this project, who will be contacted? Please provide after hours contact numbers for the appropriate people i.e. contact number of local veterinary surgeon. Please provide information in the following format where possible:

Name:Phone:Role:Comments:


55- Are any external investigators involved in this project? If so, please ensure they are listed on the Coversheet and upload a signed External Researchers Declaration to the Document’s tab prior to the submission of this application.


56- Will facilities of another institution be used? If so, please describe how.


57- Do any actual or potential interests exist, including any financial interest or other relationship or affiliation, that may affect judgements and decisions regarding the wellbeing of the animals involved?



58- Please access the Team Member Table at https://www.griffith.edu.au/research/research-services/research-ethics-integrity/animal/applications-and-forms and upload the completed table to the Documents Tab. You may click on the Documents Tab now, upload the document, then return back to the Questionnaire Tab. Your responses will not be lost. Please also ensure to assign either the Chief Investigator or one of the Internal Investigators as the Responsible Investigator on the Coversheet Tab via one of the role drop down boxes. The Responsible Investigator is the person with ultimate responsibility for the care and use of animals in the project (see 2.4.5 of the Code).


61- Does this project involve endangered or threatened species or populations? Please note, an additional permit from the Queensland Department of Environment and Heritage Protection is required for work with threatened species.



61a - Provide your Queensland Department of Environment and Heritage Protection permit number (if known) and upload a copy of the permit to the Documents tab.


62- Is a Queensland National Parks and Wildlife Service (QNPWS) permit required?



62a- Provide your Queensland National Parks and Wildlife Service (QNPWS) permit number (if known) and upload a copy of the permit to the Documents tab.


63- Is a Department of Natural Resources permit required?



63a- Provide you Department of Natural Resources permit number (if known) and upload a copy of the permit to the Documents tab.


64- Is a Fisheries permit required?




64a- Provide your Fisheries permit required (if known) and upload a copy of the permit to the Documents tab.


65- Is a Great Barrier Reef Marine Park Authority Permit (GBRMPA) permit required?



65a- Provide your Great Barrier Reef Marine Park Authority Permit (GBRMPA) permit number (if known) and upload a copy of the permit to the Documents tab.


66- Is a Moreton Bay Marine Park Authority permit required?



66a- Provide your Moreton Bay Marine Park Authority permit number (if known) and upload a copy of the permit to the Documents tab.


67- Is an Environment Australia Permit (EPA) required?



67a- Provide your Environment Australia Permit (EPA) (if known) and upload a copy of the permit to the Documents tab.


68- Is a Stock Movement Permit required?



68a- Provide your Stock Movement Permit (if known) and upload a copy of the permit to the Documents tab.



69- Does this project require a scientific research and educational purposes permit (ECOACCESS)?



69a- Provide your Scientific research and educational purposes (ECOACCESS) permit number (if known) and upload a copy of the permit to the Documents tab.


70- Is an Australian Government - Department of Agriculture - Australian Quarantine and Inspection Service (AQIS) permit required?



70a- Provide your Australian Government - Department of Agriculture - Australian Quarantine and Inspection Service (AQIS) permit number (if known) and upload a copy of the permit to the Documents tab.


71- Is a prohibited matter permit required? (The Biosecurity Act 2014 lists prohibited matter in Schedule 1 of the Act).



71a- Please specify the prohibited matter permit number and upload a copy to the Documents tab.


72- Will any Schedule 8 drugs be used for this project?



72a- S8 licence details. Provide the licence number and name of holder (if known) and upload a copy of the licence to the Documents tab.



73- Are any of your permits/approvals still pending?



73a- You've indicated that you have permits/approvals still in the process of approval. Please indicate the permit/approval type and their expected approval date (if known).


The Field Research questionnaire has been completed. Please ensure you complete the 'Animal Usage' tab, and upload any necessary permits/approvals in the 'Documents' tab before hitting 'Submit' on the coversheet.




Prior Reviewed Research Questionnaire Branch6- Please provide the names of any PhD, Masters, Honours or Undergraduate Students involved in this project.


7- Please provide the name of the collaborator(s) affiliated with the other Institution/Organisation.


8- Please provide the name of the other Institution/Organisation.


9- Please provide the Scientific User Number or accreditation number of the other Institution/Organisation.


10- In what country is the other Institution/Organisation located? If overseas, please explain why animal use will be undertaken overseas rather than in Australia and provide advice on how the proposed project can meet the principles of the Code, taking into account compliance with local requirements.


11- In what state is the other Institution/Organisation is located in?


12- Please provide the name and contact details (phone and email address) for the AEC that has approved (or is currently considering) this project.

Please also upload a copy of the original application approved by the Primary AEC along with a copy of the approval notification issued to the Documents tab. You may click on the Documents tab now, upload the document, then return back to the Questionnaire tab. Your responses will not be lost.



13- Which institutional AEC will be responsible for monitoring this project? Please describe how the monitoring will take place. If more than one AEC will undertake the monitoring of this project, please specify the parts of the project that will be monitored by each AEC.


14- Which institution will produce mandatory annual report? Only ONE institution should report on each project.


15- Will any animals be held or used in Griffith facilities? If yes, please elaborate. Note: If yes, a full application to Griffith AEC may be required.


16- Briefly describe the nature of the involvement of Griffith personnel:


17- Please specify what funding source and grant number is associated with this protocol (if applicable).

Text answer – Go to the next question

The Prior Review questionnaire has been completed. Please ensure you complete the 'Animal Usage' tab, and attach any necessary permits/approvals in the 'Documents' tab.




Application Variation Request1- Describe the proposed changes to the approved application you have just made on the Coversheet and/or Animal Usage Tab and provide a reason for each change.


2- For any changes to the methods or procedures described in the approved application, please upload the entire approved protocol with all proposed changes highlighted to the Documents Tab. Please also provide a description of the proposed amendment in lay terms and specify how the proposed action(s) differ from that already approved in the original project; describe the anticipated positive and negative impact of the amendment on the animal(s), including all possible/potential risks to animal welfare and describe how any potential negative impacts will be minimised; justify why the proposed amendment is necessary to meet the aims of the project.

You may click on the Documents Tab now, upload the documents, then return back to the Questionnaire Tab. Your responses will be saved.


3- If you have added one or more investigators to this project please describe why and specify for each new member the following: the institution they are associated with, their role on the project, their experience and prior training in the procedures or activities they will perform, and if animal facility induction is required.

Please specify if the new team member has had any AEC approval withdrawn or been suspended from working with animals for any period. Ensure to provide details and relevant project numbers.

Please also upload a signed New Team Members Declaration Form, available at: https://www.griffith.edu.au/research/research-services/research-ethics-integrity/animal/animal-ethics-applications.


4- If you have removed any investigators from this project please describe why and specify the name of each investigator being removed & note which team member(s) will take on their role in the project.





5- If you have extended the end date, please describe why. Please note, extensions of more than 1 year past the original expiry date cannot be approved by the Animal Ethics Committee. If further extension is required, please submit a new application.


6- If you have made changes to the details on the Animal Usage tab, please describe these and why they are requested. Please note, if the total number of animals used will be greater than what has been approved in the original application, a new application should be submitted.

Text answer – Go to the next question



Annual/Final/Progress Report Questionnaire BranchPlease specify the type of report you wish to submit

Progress, Annual or Final Report – Go to the next question

Unexpected Adverse Event – Go to the ‘Adverse Event Report Questionnaire Branch’ section of this document.

1- Select the appropriate report type from the drop down box.

Progress Report – Go to question 2

Final Report – Go to question 2

Annual Report – Go to question 2

2- What is the current status of your project?

Not currently commenced – Go to question 3b

Not commenced and abandoned – Go to question 3a

Commenced but abandoned – Go to question 3c

Ongoing until approval expires – Go to question 4

Complete – Go to question 3d

Note: If you would like to request an extension please complete a Variation form


3a- Specify the date of abandonment (dd/mm/yyyy)


3b- Please comment on the abandonment or delay of this project (if applicable) and note any Variations that may be required.

Note: Even though no animal use has occurred during this reporting year, fields in the Animal Usage tab must be completed (zeros added) to allow for accurate reporting to the regulator.


3c- Specify the date of abandonment (dd/mm/yyyy)



3d- Specify the date of completion (dd/mm/yyyy)


4- If the approved number of animals were not used, please provide a brief explanation as to why.


5- Has this project been conducted according to the approved protocol?


6- Please comment on what progress has been achieved and any subsequent Variations that may be required


6a- Please comment on what progress was made and the cause of the abandonment.


7- Has the well being of animals been consistent with that anticipated in the approved application?


No – Go to question 7a

7a- Please provide details of those risks that were not addressed and the actions taken by the research team


8- Have the animals used in this project not been held at Griffith University and reported to another AEC? (i.e. Is this a Prior Review project?)



8a- Please complete the Animal Usage information for each species/strain that you are approved to use on the Animal Usage tab. Please note, animals as defined in the QLD Animal Care and Protection Act need to be reported. These include:



- Any live vertebrate including amphibians, birds, fish, mammals, reptiles (but not human beings).

- All species of the class Cephalopoda (octopi, squid, cuttlefish and nautilus).

- Live pre-natal or pre-hatched creatures in the last half of gestation, e.g. a mammalian or reptilian foetus, pre-hatched avian, mammalian or reptilian young and live marsupial young. However, a human foetus is not an animal, nor are the eggs, spat, or spawn of fish.

- The remains of an animal that was killed for a scientific purpose is also considered to be an animal.

Note: Even if no animal use occurred during the reporting year, fields in the Animal Usage tab must be completed to allow for accurate reporting to the regulator.


9- Please provide a 200-400 word description of results achieved according to your project's aims. Include discussion of publications and other outputs (e.g. curriculum change, higher degree completion, or services provided). If this is a final report, please advise on conclusions as to how procedures in future projects could be modified to reduce any impact on animal welfare.


10- Have any Animal tissues been utilised by other researchers?



11- I have included a copy of a completed animal score sheet used during the project and copies of all permits and/or permit numbers as part of this report (where applicable). Note copies of score sheets are required when an animal is held during a project.

I have included the necessary items. – Go to question 12

12- I confirm that the information provided in this report is accurate and that to date, this project has been conducted in accordance with the approved application, except where indicated otherwise, and within the governing principles of the Australian Code for the Care and Use of Animals for Scientific Purposes, and the Griffith University Guidelines for Animal Care and Use in Teaching and Research

I agree. – Go to question 13

13- Are there any ethical issues or complaints about the conduct of this project that you would like to bring to the attention of the Animal Ethics Committee?



14- Please select one of the following: I am...

The Chief Investigator – Questionnaire has been completed

A Team Member – Questionnaire has been completed



Adverse Event Report Questionnaire Branch

1- Please provide a summary of the circumstances. Include details of the symptoms and/or signs exhibited by the animal, e.g. weight loss, diarrhoea, vomiting, respiratory difficulty, collapse, abdominal swelling or other signs of injury or distress, or found dead. Also specify at what stage of the project the event occurred and what treatments/procedures had been performed on the particular animal(s) prior to the event. Include a timeline of events if relevant and date(s). Detail what action was taken when the event happened or was discovered.


2- Was there any unplanned mortality?


No – Go to question 2b

2a- How many animals died?


2b- Was there any unplanned euthanasia?

Yes – Go to question 2c


2c- How many animals were euthanised?


3- Was a post mortem conducted?

Yes – Go to question 3b


3a- Please explain why no post mortem was conducted



3b- Please provide the name of the person or company who conducted the post mortem, and detail their relationship to this study (consultant veterinarian/member of the project team)


3c- Please provide a summary of the post mortem results


4- Was there a recovery of some or all animals that were involved in the event which were then able to be used in the approved study?



4a- All animals recovered?



5- Did the event cause the experiment to be cancelled?



5a- What date did the experiment cease? (dd/mm/yyyy)


6- Are there any other details you wish to report about the event? Please upload any supporting documentation (photos/references/reports etc) on the Document’s tab. Note: If any animal tissues have been utilised by other researchers please provide details of the types of tissues that have been shared and name the investigator(s) you provided these tissues to.


7- Have any future prevention strategies/precautions been identified? Please describe



8- Will the protocol be amended as a result of this unexpected or adverse event and a Variation be submitted?

Yes – Questionnaire has been completed

No – Questionnaire has been completed

