introduction and lab rules weiwei tang [email protected] biochemistry experiment ①

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Introduction and lab Rules Weiwei TANG [email protected] Biochemistry Experiment

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Page 1: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Introduction and lab Rules

Weiwei TANG

[email protected]

Biochemistry Experiment ①

Page 2: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

2 |Biochemistry laboratory

1. Aims of Biochemistry Experiments

Experiments Rules

Instruments Introduction

Page 3: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

GRADING

Theory EXAM : 70%Attendance(Theory): 10%Lab Performance: 20%

Page 4: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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2. Experiment Rules

① Put on your white lab coat before the class begin

Biochemistry laboratory

Page 5: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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2. Experiment Rules

② It is better done than said

Biochemistry laboratory

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2. Experiment Rules

③ Make Compensation

For Any Damage

Biochemistry laboratory

Page 7: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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2. Experiment Rules

④ Please shut off

the faucet and Power

Biochemistry laboratory

Page 8: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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2. Experiment Rules

⑤ Clean the classroom before you leave!

Biochemistry laboratory

Page 9: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

If you break any rules above, I will lower your 

participation  grade !

Page 10: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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3. Instruments Introduction

① check instruments for each group by the table below

category number category number

250-ml beaker 1 Dropper 1

100-ml beaker 1 Glass stirring rod 1

Large Test tube 18 Test-tube rack 1

Small Test tube 6 Bulb for pipette 1

Biochemistry laboratory

Page 11: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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3. Instruments Introduction

Beaker

Biochemistry laboratory

Page 12: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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3. Instruments Introduction

Test tube

Biochemistry laboratory

Page 13: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Solution Mixing

Rotation

Fliping

Shaking

Biochemistry laboratory

Page 14: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

3. Instruments Introduction

Test-tube

rack

Biochemistry laboratory

Page 15: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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3. Instruments Introduction

Test-tube

brush

Biochemistry laboratory

Page 16: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Test tube Washing

1. Flush the test tube with water At least 3 times

2. Scrub it with  detergent both inside & outside

3. Flush the test tube with water at least 5 times just like step1

4. Rinse it with distilled water and place

them upside down on the rack to leave it dry

Biochemistry laboratory

Page 17: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

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3. Instruments Introduction

Dropper

Biochemistry laboratory

Page 18: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

3. Instruments Introduction

Glass-stirring rod

Biochemistry laboratory

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3. Instruments Introduction

Tube clip

Biochemistry laboratory

Page 20: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

3. Instruments Introduction

Bulb for pipette

Biochemistry laboratory

Page 21: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

3. Instruments Introduction

Scaled pipette

Biochemistry laboratory

Page 22: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Scaled Pipette Pattern:

0.5 ml 、 1 ml 、 2 ml

5 ml 、 10 ml etc.

Biochemistry laboratory

Page 23: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

• Hold Like This

Biochemistry laboratory

Page 24: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Usage

Hold pipette

Absorb Solution

Adjust the Volume

Drop Solution

Biochemistry laboratory

Page 25: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

3. Instruments Introduction

Micropipette

Biochemistry laboratory

Page 26: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Micropipette Specifications: 10 μl 、 20 μl 、 100 μl 、 200 μl 、 1000 μl & 5000 μl

Biochemistry laboratory

Page 27: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

THE INTRODUCTION ON PIPETTE

Biochemistry laboratory

Page 28: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

THE INTRODUCTION ON PIPETTE

Biochemistry laboratory

Page 29: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Usage

1. Set the volume of solution

2. Choose paired tip ,and put on it

3. Absorb solution with 1st Lock , drop solution with 2nd Lock.

Page 30: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Summary

1. Be careful

2. Be patient

3. Be lively

Biochemistry laboratory

Page 31: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

Please hand in your Report in time !

Thank youBiochemistry laboratory

Changsha Medical University

Page 32: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

LAB REPORT

Page 33: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

LAB REPORT

Page 34: Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

LAB REPORT