international quality assurance programme ......the ice programme is a unodc mandated activity and...

INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)

INTERNATIONAL COLLABORATIVE EXERCISES (ICE)

Summary ReportSEIZED MATERIALS

2016/2

Samples Substances Concentrations Comments on substancesSample 1 Cocaine 77.0 %

Sample 2 JWH-073 14.5 %

Lactose - % Quantification not required

Sample 3 Ketamine 11.4 %

Lactose -

Sample 4 Heroin 28.4 %

Lactose -

Morphine minor component -

Test Samples InformationSamples Comments on samplesSample 1 SM-1 was prepared from a seizure containing 77.0 % (w/w) Cocaine base. The test sample also

contained benzoylecgonine with methylecgonine and cinnamoyl cocaine isomers as minor components.

Sample 2 SM-2 was prepared from a seizure containing 14.5 % (w/w) JWH-073. The test sample also contained lactose.

Sample 3 SM-3 contained 11.4% % (w/w) Ketamine base. The test sample also contained lactose.

Sample 4 SM-4 was prepared from a seizure containing 28.4 % (w/w) Heroin base. The test sample also contained lactose, acetylcodeine, 6-monoacetylmorphine with morphine, noscapine and papaverine as minor components

2016/2-SM Copyright (c) 2017 UNODC

INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)

% Quantification not required



Table of contents Introduction Page 3 Comments from the International Panel of Forensic Experts Page 3 NPS reported by ICE participants Page 5

Codes and Abbreviations Page 7

Sample 1 Analysis Page 8 Identified substances Page 8

Statement of findings Page 13 Identification methods Page 22

Summary Page 27 Z-Scores Page 28 Sample 2 Analysis Page 32 Identified substances Page 32

Statement of findings Page 36 Identification methods Page 45

Summary Page 50 Z-Scores Page 51


Statement of findings Page 59 Identification methods Page 68 Summary Page 73

Z-Scores Page 74


Statement of findings Page 82 Identification methods Page 91 Summary Page 96

Z-Scores Page 97

2

Introduction An important element of the UNODC International Quality Assurance Programme (IQAP) is the implementation of the International Collaborative Exercises (ICE). The exercises allow laboratories, from both developing and developed countries, to continuously monitor their performance in drug testing on a truly global scale. This report provides information on analytical results of laboratories participating in the Seized Materials (SM) group. In order to maintain confidentiality, the participating laboratories have been assigned random “Web Codes”, which change every round. The analytical results returned by laboratories participating in ICE are evaluated by UNODC and a confidential report is provided to each laboratory on its own performance. The overall analytical results are reviewed by the UNODC’s International Panel of Forensic Experts which oversees the implementation of these exercises, and offers guidance and support in addressing relevant quality issues. The exercises provide an overview of the performance and capacity of participating laboratories and enable UNODC to tailor technical support in the laboratory sector for greatest impact. The ICE programme is a UNODC mandated activity and is implemented through regular budget funds and through the UNODC Global Scientific and Forensic Programme – Support Project (GLOU54), which operationalizes the forensic aspects of the UNODC Thematic Programme on Research, Trend Analysis and Forensics"

Comments from the International Panel of Forensic Experts Participation of Laboratories In the 2016/2 round of the ICE programme, results were submitted within both the Seized Materials (SM) and Biological Specimens (BS) test groups by 225 laboratories in 73 countries. Within the SM test group, there were 181 participating laboratories from 67 countries and within the BS test group, results were submitted by 89 laboratories from 44 countries. Qualitative Analysis The analytical technique most commonly used for screening of test samples in the SM test group was the marquis reagent (41% of participants), while GC-MS (93% of participants) was the most commonly used technique for identification/confirmation of the components in the test samples followed by FTIR (44% of participants). The results for the qualitative identification of the controlled substances in the SM test group, the number of false positive/negative results and the analyses not performed are shown in the table 1.

Table 1. Performance of participants in the 2016/2 round of ICE.

Test sample SM-1

(Cocaine) SM-2

(JWH-073) SM-3

(Ketamine) SM-4

(Heroin)

Correct identification by participants

100% 90% 99% 99%

Number of false positives 0 3 2 1

Number of false negatives 0 11 1 1

Number of Analyses not performed 0 8 0 0

While the overall number of false positive/negative results reported by ICE participants is encouragingly low, laboratories reporting false positive or false negative results should investigate the reasons for this and corrective actions should be taken in order to continuously improve performance. There were only a total of eight analyses not performed, however ICE participants should note that test samples can contain any of the substances in the ICE menu and screening and identification carried out should take this into account.

3

Quantitative Analysis The number of participants who carried out quantitative analysis, 125 (69%) for SM-1, 40 (22%) for SM-2, 94 (43%) for SM-3 and 122 (67%) for SM-4 continues to be encouraging, particularly the high percentage of participants who quantified the cocaine and heroin in SM-1 and SM-4 respectively. It is also encouraging that 129 (71%) of participants in the SM test group performed quantitation and of these, 120 (93%) quantified more than one substance and 40 (31%) quantified all four test samples. It is understood that quantitative analysis of controlled substances is often governed by local legislation and the requirements of prosecution. With regard to the analytical techniques used by participants performing quantitation, 59% used GC-FID, 26% used mass spectrometry and 25% used HPLC. z-scores obtained by participants in quantification are shown in table 2 below.

Table 2. Quantitative performance of participants in the 2016/2 round of ICE.

z-score

Test sample

SM-1 SM-2 SM-3 SM-4

(Cocaine) (JWH-073) (Ketamine) (Heroin)

|z| < 2, satisfactory 84% 85% 87% 85%

2 ≤ |z| ≤ 3, questionable 7% 10% 4% 6%

|z| > 3, unsatisfactory 9% 5% 9% 9%

According to the recommendations in ISO 13528:2005, an unsatisfactory z-score is considered to give an action signal and a questionable z-score is considered to give a warning signal. A single action signal or warning signals in two successive rounds shall be taken that an anomaly has occurred that requires investigation. Participants with z-scores outside acceptable limits should review their quantification procedures. In the 2016/1 round of ICE, 17 laboratories obtained questionable z-scores with 2 ≤ |z| < 3. Twelve of these laboratories participated and performed quantification in the 2016/2 round with all laboratories improving their performance and obtaining acceptable z-scores with |z| < 2 for at least one substance. Three of these laboratories also obtained unsatisfactory z-scores and none of these laboratories obtained questionable z-scores in 2016/2. 22 laboratories obtained unsatisfactory z-scores in ICE 2016/1 with |z| ≥ 3. Of these laboratories, 17 participated in ICE 2016/2 and performed quantification, with 16 (94%) improving their performance in at least one substance. Ten (59%) of these laboratories also obtained an unsatisfactory z-score in the 2016/2 round and 4 (24%) obtained questionable z-scores.

Laboratories that need to perform quantitation routinely are encouraged to participate regularly in external proficiency testing or collaborative exercises such as the ICE programme. This will enable laboratories to assess the quality of their quantitative methods through the z-score values obtained. In addition to the use of

z-scores as a measure of performance in quantitation, participants are encouraged to compare their results with the assigned values provided in individual quantification reports, to assess the accuracy of their quantitative performance. Laboratories reporting false positive or false negative results should investigate the root causes and corrective actions taken promptly in order to continuously improve performance. Participation in the ICE programme also helps in monitoring the effect of corrective actions. UNODC would like to acknowledge the valuable contribution of the Chemical Metrology Laboratory of the Health Sciences Authority, Singapore, for the provision of specific software used for the quantitative statistical calculations in the ICE programme.

4

New Psychoactive Substances (NPS) reported by laboratories participating in the 2016/2 round of the ICE programme During the 2016/2 round of ICE, participating laboratories provided information on NPS that had been identified in their laboratories. In total there were 225 reports of 135 substances. The most commonly reported substances were equally from the groups of synthetic cathinones (32%) and synthetic cannabinoids (32%) as shown in figure 1.

Figure 1. NPS reported by ICE laboratories during the 2016/2 round of ICE

In terms of the mode of action of the NPS reported by ICE participants, the predominant substances reported were stimulants (42%), while 31% were synthetic cannabinoid receptor agonists (SCRA’s) and 9% were synthetic opioids (which included seven fentanyl derivatives) as shown in figure 2.

Figure 2. Mode of action of NPS reported by ICE participants in the 2016/2 round of ICE

32%

32%

20%

7% 4% 2%

2%

1%

Synthetic cannabinoids

Synthetic cathinones

Others

Phenethylamines

Ketamine and phencyclidinetype substances

Tryptamines

Aminoindanes

Piperazines

42%

32%

9%

7%

6%

4%

Stimulants

SCRA's

Opioids

Hallucinogens

Dissociatives

Sedatives/Hypnotics

5

NPS most commonly reported by laboratories participating in ICE 2016/2 The substances most often reported by ICE participants were the synthetic cannabinoid receptor agonists 5F-MDMB-PINACA (methyl 2-(1-(5-fluoropentyl)-1H-indazole-3-carboxamido)-3,3-dimethylbutanoate, AB-CHMINACA (N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-(cyclohexylmethyl)-1H-indazole-3-carboxamide and the synthetic opioid furanylfentanyl (N-(1-phenethylpiperidin-4-yl)-N-phenylfuran-2-carboxamide. For further details on these substances and others reported to the UNODC Early Warning Advisory, ICE participants can log on to the UNODC ICE portal and select the icon for the Early Warning Advisory on New Psychoactive substances as shown below.

6

Codes and Abbreviations

Tech Code Name140 Colorimetric reactions

141 Marquis reagent (sulphuric acid, formaldehyde)

142 Cobalt thiocyanate

150 Thin Layer Chromatography

160 High/Ultra High Performance Liquid Chromatography

161 High Performance Liquid Chromatography with diode array detection

170 Gas Chromatography NPD

171 Gas Chromatography FID

172 Gas Chromatography ECD

180 Gas Chromatography/Mass Spectrometry

190 Fourier Transform Infrared Spectrometry

200 Spectrophotometry (visible, UV)

210 Others (specify)

211 NMR

220 Microcrystal test

230 Liquid chromatography/mass spectrometry

231 Liquid chromatography/tandem mass spectrometry

(+) “positive”: Indicates that the analyte is identified; for presumptive tests (e.g. colour reactions), indicates that a positive reaction was obtained.

(-) “negative”: Indicates that the analyte is not identified.

(ANP): Analysis not performed.



7

Identified substances for Test Sample 1

Code Identified Substances0D11QZ cocaine

0HSVIE Cocaine

0LAKP0 Cocaine

0P0W03 COCAINE

10B0XJ Cocaine

1JJBJS cocaine

1YQDGQ cocain HCL

1ZKJHF Cocaine

211119 Cocaine

2PUPPP Cocaine

2QC44H Cocaine

2R20JW cocaine

33DUO3 cocaine

3PV3WL Cocaine

40HFEP Cocaine

4J4H44 Cocaine hydrochloride

55BR5Z cocaine

5MLCMA Cocaine

5ZDDX6 cocaine

7666AG cocaine

7IUIII cocaine

7JUSKK cocaine

7T7YHK Cocaine

8AHAJA cocain

8D1PTX COCAINE

8II46Y Cocaine

8J4DWT cocaine

8MV6V6 Cocaine

8Q8VEF Cocaine

8WEYGQ Cocaine

8Z8ZZZ cocaine

9994MR cocaine

9Q999K cocaine

A33Q33 Cocaine

A5GR55 Cocaine

A7LUO7 COCAINE

A8YSK1 cocaine

AEDDRS cocaine

APX4KD Cocaine

ARZMMQ Cocaine HCl

Sample 1 Analysis



8

Code Identified SubstancesASLAA5 Cocaine

AYNT4F Cocaina

B3SNNC cocaine

BARYZ2 cocaine

BFIU0G cocaine

BRVRR4 Cocaine hydrochloride

C9CDOR cocaine

CANEE8 cocaine

CCIVVK cocaine

CJWZIJ Cocaine

CRSEZI cocaine

CU6JGV COCAINE

CWEOYI cocaine

CZGHZZ cocaïne

DMM5Z5 cocaine

DNNSKS Cocaine

DWRNDI cocaine

E2OA5N cocaine

E3MIHV cocaïne

E5LDLX cocaine

E66LY6 cocaine

EHJ60F COCAINE 76.3 %

EMJ8VM cocaine

EXZYYP Cocaine HCl

FK5BHB Cocaine

FMFLFF cocaine

G0B9NL COCAINE HYDROCHLORIDE

G2GBDV cocaine

GGA8CG Cocaine Hydrochloride

GJIRPN Cocaine

GKHDEM cocaine

GNUILW cocaine

GUDEBT COCAINE

GV2PGK cocaine hydrochloride

H13HRX COCAINE CHLORIDE

HEHCMO Cocaine

HF3FYI cocaine

HJLSL6 Cocaine

HRYNGY COCAINE

IMVADS Cocaine

IQYQE4 Cocaine

IRWMIX Cocaine

IWA0AA Cocaine

IXUAHB Cocaine Hydrochloride



9

Code Identified SubstancesIZLLW0 cocaine

JCUITV cocaine

JEVX45 cocaine

JKG5E5 cocaine

JM2GKM cocaína

JSZ6XW Cocaine

K3S33Y Cocaine

KBUURH cocaine hydrochloride

KKKWIC cocaine

KSQKKK cocaine

L4444V COCAINE-HCl

LDDCDD Cocaine

LL8LML Cocaine

LLL1RB Cocaine hydrochloride

LNUUEI cocaine

LQ8989 cocaine hydrochloride

LVVWC7 cocaine

M1XUWT Cocaine

M72KOQ Cocaine

MCCCCC Cocaine

MDDETU Cocaine

MDQMM4 Cocaine

MMMMT5 Cocaine

MOFYQQ cocaïne

MOKSHS cocaine

MWXNUW Cocaine

MZMHCW Cocain

N8BTCF Cocaine

NWJDJI Cocaine

NZOTC6 Cocaine

O4KFNX cocaine

O888ML Cocaine

ODOOO2 cocaine hydrochloride

OJFEOO cocaine

OK66R6 cocaine

OO9QOO Cocaine

OQ4QQQ cocain ,benzoylecgonine

OS838A cocaine

OTSOQ9 cocaína

P0FZ8V cocaine

PHWNRB Cocaine

PPLICW Cocaine

PQQKLQ COCAINE

QQQQJW cocaine



10

Code Identified SubstancesR7BBLB Cocaine

RI3K33 cocaine HCl

RJINCE Cocaine

RJNRSN Cocaine

RU8UQS cocaine

RVP7VW cocaine

SIFXH3 Cocaine

SMYNNU Cocaine

SUUW3G Cocaine

SVGG03 Benzoylecognine, cocaine & ecognine methyl ester

T2DI1R Cocaine

T4VMD2 Cocaine

TI22ZK Cocaine

TQFFPF Cocaine

TQMVZE Cocaine

TRVLKK Cocaine

TSSRQR Cocaine

TSW3IE COCAINE

TZOONA COCAINE hcl

UFK6EF cocaine HCl

UG88A8 COCAINE

UJWYPY cocaine

UMXXSY Cocaine

UUUOU9 cocaine

UWBOLU cocaine

UXVVAC COCAINE

UYFCCR COCAINE

UYJBJJ Cocaine

V011CO Cocaine

VBEY7Y Cocaine

VFC8CC cocaine

VIFKAW cocaine

VPHJRP COCAINE

VVIRG4 Cocaine

VVN4CQ cocain

W0HPES

WGZNNM Cocaine

WMTTV7 Cocaine

WQOZYA cocaine

X0X444 cocaine

XEE66N cocaine

XNNNU1 Cocaíne (Hydrochloride)

XXFX8D Cocaine

YGQE84 Cocaine, Ecgonine methyl ester and Cinnamoylcocaine



11

Code Identified SubstancesYLHIIH COCAINE

YQYLYY cocaine

Z2BIDB cocaine

Z8T8N1 cocaine

ZCCQMZ Cocaine

ZQHUPQ Cocaine hydrochloride

ZWL0DM Cocaine

ZXGCGG Cocaine



12

Code Cocaine 0D11QZ +

cocaine base 49%

0HSVIE +

0LAKP0 +The sample contains cocaine (73.9%)

0P0W03 +A color test (SCOTT-Cobalt thiocyanate TEST) with positive reaction to Cocaine, to the TLC was used as a solvent for the preparation of the samples the Dichloromethane and as eluent methanol, used an aluminum plate 20 × 20 cm. Confirmation of results were made on the equipment of THERMO SCIENTIFIC NICOLET Is 10 and NICOLET Is 50 FT-IR.In relation to 2016/2 sample/SM-1, all tests are in compliance and the identified substance is cocaine.

10B0XJ +Confirmatory test (GCMS) shows test sample contains cocaine

1JJBJS +

1YQDGQ +impurites of cocain ( benzoylecgonin, cinnamoylcocain cis, trans, cocaethylen, ecgonine methyl ester, benzoic acid)

1ZKJHF +Examination revealed the presence of Cocaine

211119 +Results of screening test were positive for cocaine. Relation time and fragmentation pattern obtained with sample are equivalent to those obtained with cocaine. Cocaine was detected at 75.41 % by weight.

2PUPPP +Sample contains 78.7 ± 3.0 % (w/w) Cocaine base.

2QC44H +The sample obtains with positive result with Cobalt thiocyanate reagent.TLC : The Rf sample obtain with the same Rf Cocaine Standard.GC : The Rt sample obtain with the same Rt Cocaine Standard.

2R20JW +cocaine chloridrate 73.97% as base

33DUO3 +cocaine was as hydrochloride form.

3PV3WL +The sample SM1 contained 80,1 % of cocaine. Esthers of cocaine was in the sample SM1 identefied.

40HFEP +Mass spectrometry profile correlation between sample and reference material certified

4J4H44 +Cocaine hydrochloride confirmed in 1 unit tested of 1 unit received.

55BR5Z +./.

5MLCMA +Sample 2016/2/SM-1 found to contain Cocaine

5ZDDX6 +the sample contain cis and trans cinnamoylcocaine, pseudoecgonine

7666AG +

Statement of findings for Test Sample 1



13

Code Cocaine 7IUIII +

The result of the sm 1 (123)tests received has confirmed that the mentioned simple contains cocaine with 70.95 percentage by weight

7JUSKK +

7T7YHK +On analysis, I found sample SM-1 to contain Cocaine.

8AHAJA +

8D1PTX +

8II46Y +Cocaine was identified by GC-MS.

8J4DWT +82% +/-6

8MV6V6 +In the test sample 2016/2/SM-1 was detected cocaine

8Q8VEF +

8WEYGQ +White powder containing cocaine.

8Z8ZZZ +

9994MR +White powder, usual smell, totally soluble in water, precence of chloride ions

9Q999K +Sampel SM_1 was found to contain cocaine.

A33Q33 +Cocaine confirmed

A5GR55 +ecgonidine, methyl estercinnamoylcocaine

A7LUO7 +BY GC-MS AND FTIR

A8YSK1 +Cocaine 80.1%

AEDDRS +found cocaine with 77.7%ww

APX4KD +The white powder was analysed and found to contain cocaine hydrochloride.

ARZMMQ +

ASLAA5 +THE SAMPLE WAS FOUND TO CONTAIN COCAINE

AYNT4F +De análisis realizados se concluye que la sustancia se trata de cocaina

B3SNNC +The sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule I 2. (2) Cocaine (benzoylmethylecgonine)



14

Code Cocaine BARYZ2 +

BFIU0G +The test sample number SM-1 containing a psycho narcotic drug cocaine.

BRVRR4 +Examination and analyses performed on the white solid in SM1 showed it to contain cocaine hydrochloride (a central stimulant). Cocaine hydrochloride is a salt of cocaine. Assay SM1 = 82.6% cocaine hydrochloride

C9CDOR +

CANEE8 +

CCIVVK +The sample contain cocaine.

CJWZIJ +The sample contained 83.0% of cocaine.

CRSEZI +ecgonine methyl estercinnamoyl cocainenor-cocaine

CU6JGV +

CWEOYI +after chemical analysis was performed the sample was contain 93.8% cocaine

CZGHZZ +

DMM5Z5 +Screeninhg test with cobaltthiocyanate test positive and confirmation with GCMS.

DNNSKS +Ecgonine methyl ester, Ecgonine benzoylester, Cinnamoylcocaine

DWRNDI +cocaine, benzoylecgonine

E2OA5N +

E3MIHV +142: apparition d'une coloration bleue (Réactif 1); Décoloration en Rose (réactif 2), apparition d'une coloration bleue foncés de la couche inférieure.150: le rf du spot présent dans l'échantillon est non différentiable de celui du spot donné par le standard de cocaïne

E5LDLX +cocaine: 75.1%ww

E66LY6 +

EHJ60F +

EMJ8VM +Sample 2016/2/SM-1 contains cocaine, a dangerous drug. It also contains cinnamoylcocaine isomers, benzoyl ecgonine and methyl ecgonine.



15

Code Cocaine EXZYYP +

The sample was performed by cobalt thioyanate test,then confirmed by TLC and GC/MS. Guantified by GCFID, the sample contained cocaine 78.1% as base.

FK5BHB +After analycing the simple with colorimetric tests, GC/MS confirmation and subsequent quantification by GC-FID we can say that this simple contains 82.38% in cocaine base

FMFLFF +cocaine

G0B9NL +Off-white powder 229 milligrams 1 package COCAINE HYDROCHLORIDE (88%) Class A [Note to UNODC we report salt form purities where salt form has been identified analytically]

G2GBDV +cocaine is controlled by K.B. of 31/12/1930, changed by K.B. of 8/10/1963

GGA8CG +A sealed glass vial containing a white powder. This powder contained an average of 88% Cocaine Hydrochloride.

GJIRPN +Cocaine is a Class A controlled drug subject to the provisions of the Misuse of Drugs Act 1971.

GKHDEM +cocaine was detected by gc-ms screening and it was quantified by gc-fid using calibration curve

GNUILW +List I of Single Convention on Narcotics drugs,1961.

GUDEBT +Sample 2016/2/SM-1 a white powder gave a positive result for cocaine a controlled substance under national legislation on drugs.

GV2PGK +Cocaine hydrochloride is present (75% w/w as base) with benzoylecgonine and cis-trans cinnamoylcocaine.

H13HRX +ECGONIDINE METHYL ESTHER, ECGONINE METHYL ESTHER, CINNAMOYL COCAINE

HEHCMO +A.p. of trans-cinnamoylcocaine and cis-cinnamoylcocaine

HF3FYI +cocaine, concentration approx 80 % as free base

HJLSL6 +Sample contained cocaine as hydrochloride, and small amounts of cis and trans cinnamoylcocaine. Identification of cocaine by FTIR and MS spectrum, quantification by GC/FID

HRYNGY +POWDER

IMVADS +SM-1 contains a drug: cocaine, 79,8%

IQYQE4 +

IRWMIX +Sample 2016/2/SM-1 consists of white powder. Cocaine, a prohibited substance included in the National Law forNarcotics, is found to be present in this sample.

IWA0AA +The sample contains cocaine which is listed as a controlled drug according to french order, appendix I of February 22nd 1990.



16

Code Cocaine IXUAHB +

229 milligrams - Cocaine Hydrochloride (91%) - Class A

IZLLW0 +The sample contains a controlled substance as cocaine

JCUITV +

JEVX45 +White powder containing 72.2%ww in cocaine

JKG5E5 +cocaine hydrochloride

JM2GKM +Pruebas colorimétricas con Marquis, ScottPrueba de MicrocristalesGC-MS y FT-IR

JSZ6XW +The above article was examined and found to consist of a brown glass vial containing a quantity of white powder. The powder, which weighed 0.22 grams, was analysed and found to contain Cocaine.A sample of the powder was further analysed and found to contain 78% (weight for weight) Cocaine.Cocaine is a class A controlled drug within the provisions of the Misuse of Drugs Act 1971.

K3S33Y +The sample contains cocainehydrochloride. The content of cocainehydrochloride is determined to 95 %. Cocaine is a controlled substance in X.

KBUURH +cocaine base 78,26 %

KK6WBZ +

KKKWIC +2016/2/SM-1 = CocaineThe sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule I 2. (2) Cocaine (benzoylmethylecgonine)

KSQKKK +In the sample was found cocaine

L4444V +The sample fas found to contain COCAINE and : anhydrecgonine methyl ester - Methyl ecgonine - cinnamoylcocaine, as minor components

LDDCDD +Blue colour by Cobalt Thiocyanate test indicates the possible presence of cocaine in sample 1.Then TLC result and GCMS result confirmed that the presence of cocaine in SM 1.

LL8LML +Cocaine was detected with a purity of approximately 82% (as base)

LLL1RB +One glass bottle containing 0.23 grammes of a powder containing 0.18 grammes of cocaine.

LNUUEI +the powder is cocaine hydrochloride. The concentration of cocaine, as base, is 80.1% by weight

LQ8989 +the item is powder containing cocaine hydrochloride 74.3% purity

LVVWC7 +



17

Code Cocaine M1XUWT +

Powder (sample) contains Cocaine

M72KOQ +Traces of Cinnamoylcocaine.

MCCCCC +From screening test using (CI) and ion scan and from confirmatory test using (GC-MS) the sample was found to contain the controlled drug cocaine.

MDDETU +Sample contains 76,6 % of cocaine

MDQMM4 +Methylecgoninecinnamoilcocaine

MMMMT5 +The mixture contains cocaine and some form of sugar

MOFYQQ +positive

MOKSHS +

MWXNUW +The Test sample 2016/2/SM-1 was analyzed by Colour test and Thin Layer Chromatography technique and foundto contain Cocaine.

MZMHCW +It contains cocain in a purity of 75.1%. It also contains lactose

N8BTCF +This sample contains cocaine and has no diluent.

NWJDJI +Based on the above analysis, the sample was found to contain cocaine.

NZOTC6 +-

O4KFNX +White powder containing 81.0%ww cocaine

O888ML +The test sample 2016/2/SM-1 was found to contain 70.5% controlled drug cocaine.

ODOOO2 +The presence of drug was not as always confirmed by FTIR spectroscopy analysis because of equipment failure.

OJFEOO +Cocaina is included in Table II Schedule I drugs (narcotics use by medicine) approved by Government Decision of Republic of Moldova no. 1088 of 05.10.2004 (Last Updated 5/13/2016) (Tables and lists of narcotic drugs,psychotropic substances and their precursors under control)

OK66R6 +Only the cocaine can be identified.

OO9QOO +

OQ4QQQ +

OS838A +The sample contains cocaine which is scheduled as illicit drug in France.



18

Code Cocaine OTSOQ9 +

cocaína clorhidrato , polvo fino blanco. 83.31%

P0FZ8V +

PHWNRB +The sample was found to contain 78.5% of cocaine (as base).

PPLICW +The sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule I 2. (2) Cocaine (benzoylmethylecgonine)

PQQKLQ +THE SAMPLE 2016/2/SM-1 WAS FOUND TO CONTAIN COCAINE.

QQQQJW +

R7BBLB +Cocaine (80 % w/w) in mixture with non-specified saccharides

RI3K33 +Cocaine was identified in SM1. In addition Ecgonidine methyl ester, cis- and trans-cinnamoylcocaine and p-fluorococaine were identified. Analysis revealed that percentage of cocaine is 51.80%.

RJINCE +Ergonidine,Cinnamoylcocaine

RJNRSN +Cocaine detected in sample 2016/2/SM-1. Quantitative analysis not performed.

RU8UQS +we have found results from swg-drug library, which the last version it, and we have studied for quantitative analysies with GC-FID.

RVP7VW +

SIFXH3 +Sample 2016/2/SM-1 contains Cocaine.

SMYNNU +The sample consist controlled drugs - Cocaine

SUUW3G +ontained cocaine, cinnamoilcocaine, cis-cinnamoilcocaine and trace of methylecgonidine ,

methylecgonineSVGG03 +

The sample cnotains 78.28 % cocaine & also it contains benzoylecgonine & ecognine methyl ester .

T2DI1R +The chemical test and the coincidence in the retention time and mass fragmentogram with the reference material in the same conditions that the analyzed sample, indicate the cocaine presence.

T4VMD2 +based on GC/MS analysis, cocaine had been identified

TI22ZK +x grams of powder containing 75% cocaine. Class A

TQFFPF +Analyses performed on Item # 1 revealed presence of Cocaine.

TQMVZE +In the sample 1 we have faund cocaine. Cocaine is on the list of the drug in Montenegro.



19

Code Cocaine TRVLKK +

SM1 contains Cocaine; Sample also contains benzoylecgonine, methylesterecgonine, cis/trans-cinnamoylcocaine and norcocaine as minor components

TSSRQR +

TSW3IE +ANALYZED WHITE POWDER CONTAINS COCAINE

TZOONA +COCAINE, ANHIDROMETHYLECGONINE, NORCOCAINE, CIS & TRANS CINNAMOILCOCAINE. BESE COCAINE 75,8 +/-2,7

UFK6EF +The sample contains cocaine.Cocaine is sheduled as illicit drug.

UG88A8 +WHITE CRYSTALINE MATERIAL THAT CONTAINS DRUG COCAINE.

UJWYPY +

UMXXSY +Cocaine, Ecgoninemethylester, Ecgonidine methylester, Cis-cinnamozlcocaine, Trans-cinnamozlcocaine, Norcocaine- GCMS - Compare massspectra with library retention time of reference sample and FTIR-Cocaine - compare with IR spectrum library

UUUOU9 +

UWBOLU +the white powder contains cocaine (77.0%ww) wich is controlled by national law

UXVVAC +Presence of cocaine hydrochloride was detected in the sample. The sample also containedmultitude of trace level alkaloid impurities:(cis/trans)cinnamoylcocaine, benzoylecgonine,methylecgonine, norcocaine.

UYFCCR +IN SAMPLE 2016/2/SM-1 COCAINE WAS FOUND.

UYJBJJ +This sample is cocaine.

V011CO +The high quality of measurement results (99%) proves the presence of cocaine in the sample.

VBEY7Y +It is usually detected in our laboratory. High purity, characteristic of important seizures.

VFC8CC +

VIFKAW +the sample contains cocaine.

VPHJRP +The sample containing 74.5% cocaine base :also contain Benzoylecgonine

VVIRG4 +Samples with high concentrations of Cocaine equivalent to the samples seized in Viet Nam

VVN4CQ +cocain-HCL

W0HPES +



20

Code Cocaine WGZNNM +

WMTTV7 +The sample contains cocaine.

WQOZYA +cinnamoylcocaine

X0X444 +white powder containing cocaine and ecgonidine methylester, ecgonine methylester as cocaine impurities

XEE66N +the sample is containing cocaine and percentage of pure cocaine in it is 77.9%

XNNNU1 +Powder containing Cocaine Hydrochloride 64,7%Legal status: Illegal - Portuguese Drug law 15/93

XXFX8D +The sample found to contain Cocaine

YGQE84 +Cobalt Thiocyanate (CT) positive. GC/MS Retention time and mass charge (m/z) similar characteristics with the substances used as standard or pattern

YLHIIH +BENZOILECGONINE AND ECGONINE METYL ESTER

YQYLYY +Presence of cocaine, 66.80% concentration, with traces of cinnamoylcocaine and benzoylecgonine

Z2BIDB +On analysis, I found that the substances contain 77.9% w/w of cocaine.

Z8T8N1 +

ZCCQMZ +Cocaine HCl

ZQHUPQ +It has been detected the presence of cocaine hydrochloride in the sample

ZWL0DM +not

ZXGCGG +The identification was realized with prelimimary color test with cobalt thiocyanate and silver nitrate, the confirmative assay with FT-IR and CG-MS and the quantitative analysis was carried by GC-FID.



21

Identification methods for Test Sample 1

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

0D11QZ SI I

0HSVIE SI

0LAKP0 S S S I I

0P0W03 S S I I

10B0XJ SI SI I SI Fast blue B salt

1JJBJS SI

1YQDGQ SI

1ZKJHF SI

211119 S S I

2PUPPP S I SI SI

2QC44H S S S I

2R20JW S S S I

33DUO3 SI I

3PV3WL SI I

40HFEP I I

4J4H44 I SI I

55BR5Z I SI SI

5MLCMA S I

5ZDDX6 S SI I I I I

7666AG I SI

7IUIII S S S I I

7JUSKK S S SI S fast blue B

7T7YHK S I I

8AHAJA S I I

8D1PTX SI SI I

8II46Y S S S I I

8J4DWT S I

8MV6V6 I

8Q8VEF I S

8WEYGQ S I SI

8Z8ZZZ S S I I

9994MR I SI

9Q999K S I

A33Q33 S S SI I

Analytical Technique Codes

Legend: S ... used for Screening I ... used for Identification SI ... used for both



22

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

A5GR55 SI I

A7LUO7 S S I I

A8YSK1 S I

AEDDRS SI

APX4KD S I I

ARZMMQ I SI

ASLAA5 S S I I

AYNT4F S S I I S Macroscopic

B3SNNC SI I

BARYZ2 SI I

BFIU0G S S I I

BRVRR4 S S S SI I I

C9CDOR S I

CANEE8 SI

CCIVVK S SI I SI

CJWZIJ SI I I UPLC-PDA

CRSEZI I I SI I

CU6JGV SI S SI I

CWEOYI I S I

CZGHZZ SI I

DMM5Z5 S I I

DNNSKS S I I I

DWRNDI I SI I

E2OA5N SI SI SI I

E3MIHV S S S I

E5LDLX I SI

E66LY6 S S S I I S ac. nitrico

EHJ60F S S S SI I SI

EMJ8VM S S S I I I Duquenois-Levine Test

EXZYYP S S S I I SI

FK5BHB S S I I Duquenois

FMFLFF SI I

G0B9NL S S S I I I

G2GBDV I S

GGA8CG S S I I S Basic Cobalt thiocynanate (for ketamine)

GJIRPN S S I

GKHDEM SI

GNUILW I SI

GUDEBT S S I I



23

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

GV2PGK I I

H13HRX SI SI S SI I I S Benedict

HEHCMO S S S SI I

HF3FYI SI I

HJLSL6 I I SI

HRYNGY SI

IMVADS S S S I I S

IQYQE4 I I

IRWMIX S S I I

IWA0AA I I

IXUAHB S S S I I

IZLLW0 S I S S I

JCUITV S S SI

JEVX45 I I SI

JKG5E5 SI S I SI

JM2GKM S S S I I I

JSZ6XW S I

K3S33Y I I

KBUURH SI I I

KK6WBZ I SI

KKKWIC SI I

KSQKKK S S I I I

L4444V S SI I

LDDCDD S S SI I

LL8LML S S I I

LLL1RB S S I SI I I Raman Spectroscopy

LNUUEI I I SI I

LQ8989 I

LVVWC7 S I SI I

M1XUWT I

M72KOQ I SI

MCCCCC S S I

MDDETU SI

MDQMM4 I SI

MMMMT5 S I I S

MOFYQQ S I I S Drug Testing Kits

MOKSHS I SI

MWXNUW

S S SI S Fast Blue B Salt Test

MZMHCW S I



24

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

N8BTCF I S I I

NWJDJI S I I

NZOTC6 SI

O4KFNX I I SI

O888ML S S S SI I

ODOOO2 S I I I

OJFEOO S S S I I I

OK66R6 S I SI I

OO9QOO SI SI

OQ4QQQ S S I SI

OS838A S S I

OTSOQ9 S I I S

P0FZ8V SI

PHWNRB SI

PPLICW SI I

PQQKLQ S I SI

QQQQJW I

R7BBLB I SI I I S Raman

RI3K33 S S I I I S Fast Blue B salt test

RJINCE S S I I

RJNRSN S SI I

RU8UQS S S I SI

RVP7VW SI SI SI I

SIFXH3 S S S I I

SMYNNU I SI I

SUUW3G SI

SVGG03 SI I

T2DI1R S S I

T4VMD2 S I

TI22ZK S S I

TQFFPF S S I I

TQMVZE S S I I I

TRVLKK I S

TSSRQR SI I

TSW3IE S S I I

TZOONA S S S I I I I XRD

UFK6EF I SI I

UG88A8 SI S SI SI S Simon

UJWYPY S SI SI I



25

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

UMXXSY S S SI I

UUUOU9 SI SI SI I

UWBOLU I SI

UXVVAC S I I I I

UYFCCR I SI

UYJBJJ S I I

V011CO S S I I

VBEY7Y SI S S I I I

VFC8CC SI

VIFKAW SI I

VPHJRP S I I I I HPLC/RID

VVIRG4 S I I I LC-MS-TOF

VVN4CQ I SI SI

W0HPES I SI I

WGZNNM I SI

WMTTV7 SI SI

WQOZYA SI I

X0X444 SI I

XEE66N SI S S SI I

XNNNU1 S S I

XXFX8D S S I S Janovsky

YGQE84 S S S I I

YLHIIH I SI

YQYLYY I

Z2BIDB S S S I I I Raman Spectrometer

Z8T8N1 SI I

ZCCQMZ I SI I

ZQHUPQ I S I

ZWL0DM SI I

ZXGCGG S S S I I



26

RESPONSE SUMMARY FOR TEST SAMPLE 1

Participants: 181

The response summary of all 181 participating laboratories for the identification of SM-1, frequency of use of screening and identification techniques and false positives and negatives:

Identification of Test Sample-1:

Substance Name Number of laboratories correctly identifying: Cocaine 181

False positives for Test Sample-1:

Round Code: Substance reported: Tech Code: 0

False negatives for Test Sample-1 (techniques(s) used for ANP):

Round Code: Substance Name: Tech Code: 0

Frequency of use of screening, identification and quantification techniques:

Response Summary

Participants 181

Technique 140 141 142 150 160 161 170 171 172 180 190 200 210 211 220 230 231

Screening (No.) 54 75 62 19 0 3 0 4 0 72 19 1 13 0 1 2 0

(%) 29.8 41.4 34.3 10.5 0.0 1.7 0.0 2.2 0.0 39.8 10.5 0.6 7.2 0.0 0.6 1.1 0.0

Identification (No.) 13 6 5 34 5 12 1 35 1 168 79 4 8 3 0 7 4

(%) 7.2 3.3 2.8 18.8 2.8 6.6 0.6 19.3 0.6 92.8 43.6 2.2 4.4 1.7 0.0 3.9 2.2

Quantification (No.) 0 0 0 0 8 24 0 76 0 30 0 0 1 3 0 1 0

(%) 0.0 0.0 0.0 0.0 6.2 18.6 0.0 58.9 0.0 23.3 0.0 0.0 0.8 2.3 0.0 0.8 0.0

2016/ -SM Copyright (c) 201 UNODC27

Z‐Score Report per Sample per Substance

Round: 2016/2 Robust average = 77.3 %Substance: SM‐1; Cocaine UNODC value = 77.0 %

‐13.3

‐6.5

‐5.9

‐5.3

‐4.5

‐4.0

‐3.5

‐2.9 ‐2.8

‐2.4 ‐2.2

‐1.7 ‐1.6

‐1.6

‐1.6 ‐1.5 ‐1.5

‐1.4 ‐1.3 ‐1.2

‐1.2

‐1.2 ‐1.1 ‐1.0 ‐0.9 ‐0.9 ‐0.8 ‐0.8

‐0.8

‐0.8 ‐0.7

‐0.7

‐0.7 ‐0.7

‐0.6 ‐0.6 ‐0.5

‐0.5

‐0.5

‐0.5

‐0.5

‐0.5

‐0.5 ‐0.4 ‐0.4 ‐0.3

‐0.3 ‐0.3 ‐0.2 ‐0.2 ‐0.1

‐0.1

‐0.1

‐0.1

‐0.1 0.0

0.0 0.0

0.1

0.1 0.1 0.1 0.1

0.1 0.1

0.1

0.1 0.2

0.2

0.2

0.2 0.2

0.2 0.2

0.2

0.2 0.3 0.3 0.3

0.3 0.4

0.4 0.4

0.4

0.4 0.4

0.4 0.5

0.5 0.5 0.5

0.5

0.5

0.6 0.6 0.6 0.7

0.7

0.7 0.7 0.7 0.8 0.8 0.9

0.9 0.9 1.0 1.1

1.1 1.2 1.3 1.4 1.5 1.5 1.6 1

.82.2 2.4

2.6 2.7 2

.93.43.7 3.8

4.9

‐4.0

‐3.0

‐2.0

‐1.0

0.0

1.0

2.0

3.0

4.0

5ZDDX

60D

11QZ

RI3K

33ZXGCG

GXN

NNU1

4J4H

447666

AGE66LY6

VFC8

CCYQ

YLYY

W0H

PES

DWRN

DI

E2OA5

NO88

8ML

KSQKK

KIZLLW0

7IUIII

HRY

NGY

VVIRG4

GKH

DEM

JEVX

45UXV

VAC

DNNSKS

RU8U

QS

33DUO3

JM2G

KMBR

VRR4

0LAK

P0JKG5E5

2R20JW

8II46Y

ZQHUPQ

LQ89

89RV

P7VW

VPHJRP

HJLSL6

GV2

PGK

OS838A

TI22ZK

ODOOO2

E5LD

LXMZM

HCW

C9CD

OR

2111

19JCUITV

SUUW3G

TZOONA

KK6W

BZEH

J60F

MDD

ETU

A5GR5

5AR

ZMMQ

MOKSHS

T2DI1R

UWBO

LUSM

YNNU

CRSEZI

40HFEP

8Z8ZZZ

P0FZ8V

LVVW

C7AE

DDR

S1JJBJS

UFK6EF

X0X4

44XEE66N

Z2BIDB

GGA8

CGGNUILW

JSZ6XW

VIFKAW

8MV6

V6EXZYYP

HEH

CMO

KBUURH

SVGG03

ZCCQ

MZ

PHWNRB

2PUPP

PTSSRQR

BARY

Z2UYJBJJ

G2G

BDV

G0B

9NL

ZWL0DM

55BR

5ZWGZN

NM

RJINCE

IQYQ

E4LLL1RB

GJIR

PNR7

BBLB

UUUOU9

HF3FYI

IMVA

DSCA

NEE8

3PV3

WL

A8YSK1

LNUUEI

OO9Q

OO

IWA0

AACZGHZZ

CU6JGV

IXUAH

BO4K

FNX

TQMVZ

EVB

EY7Y

8J4D

WT

LL8LML

FK5B

HB

CJWZIJ

OTSOQ9

1YQDGQ

7JUSKK

8Q8V

EFK3

S33Y

NZO

TC6

A7LU

O7

MDQ

MM4

Z8T8N1

9994

MR

WQOZYA

H13HRX

CWEO

YI10B0

XJ

z‐scores can be interpreted by participants in line with ISO 13528:2005, secion 7.4.2 and ISO/IEC guide43‐1:1997(E) as follows:IzI 3 = unsatisfactory

According to the recommendations in ISO 13528:2005, an unsatisfactory z‐score is considered to give an action signal and a questionable z‐score is considered to give a warning signal. A single action signal or warning signal in two successive rounds shall be taken that an anomaly has occured that requires inverstigation.

28


Round: 2016/2Substance: SM‐1; Cocaine

-10

-8

-6

-4

-2

0

2

4

6

8

10

z-sc

ore

Dispersion of z-scores using different analytical techniques for the quantification of cocaine in 2016/2 SM-1

HPLC GC‐FID GC‐MS

29

Z-Scores for Test Sample 1



Concentration (mass%)

Deviation from robust average

z-scoreConcentration

(mass%)

Deviation from robust

averagez-score

5ZDDX6 19.6 -57.7 -13.3 ARZMMQ 77.0 -0.3 -0.10D11QZ 49.0 -28.3 -6.5 MOKSHS 77.0 -0.3 -0.1RI3K33 51.8 -25.5 -5.9 T2DI1R 77.0 -0.3 -0.1ZXGCGG 54.1 -23.2 -5.3 UWBOLU 77.0 -0.3 -0.1XNNNU1 57.6 -19.7 -4.5 SMYNNU 77.2 -0.1 0.04J4H44 60.0 -17.3 -4.0 CRSEZI 77.3 0.0 0.07666AG 62.0 -15.3 -3.5 40HFEP 77.4 0.1 0.0E66LY6 64.7 -12.7 -2.9 8Z8ZZZ 77.5 0.2 0.1VFC8CC 65.2 -12.1 -2.8 P0FZ8V 77.5 0.2 0.1YQYLYY 66.8 -10.5 -2.4 LVVWC7 77.6 0.3 0.1W0HPES 67.6 -9.7 -2.2 AEDDRS 77.7 0.4 0.1DWRNDI 70.0 -7.3 -1.7 1JJBJS 77.8 0.5 0.1E2OA5N 70.5 -6.8 -1.6 UFK6EF 77.8 0.5 0.1O888ML 70.5 -6.8 -1.6 X0X444 77.9 0.6 0.1KSQKKK 70.5 -6.8 -1.6 XEE66N 77.9 0.6 0.1IZLLW0 70.7 -6.6 -1.5 Z2BIDB 77.9 0.6 0.17IUIII 71.0 -6.3 -1.5 GGA8CG 78.0 0.7 0.2

HRYNGY 71.0 -6.3 -1.4 GNUILW 78.0 0.7 0.2VVIRG4 71.5 -5.8 -1.3 JSZ6XW 78.0 0.7 0.2GKHDEM 72.1 -5.2 -1.2 VIFKAW 78.0 0.7 0.2JEVX45 72.2 -5.1 -1.2 8MV6V6 78.1 0.8 0.2UXVVAC 72.2 -5.1 -1.2 EXZYYP 78.1 0.8 0.2DNNSKS 72.5 -4.8 -1.1 HEHCMO 78.2 0.9 0.2RU8UQS 73.0 -4.3 -1.0 KBUURH 78.3 1.0 0.233DUO3 73.2 -4.1 -0.9 SVGG03 78.3 1.0 0.2JM2GKM 73.5 -3.8 -0.9 ZCCQMZ 78.4 1.1 0.3BRVRR4 73.8 -3.5 -0.8 PHWNRB 78.5 1.2 0.30LAKP0 73.9 -3.4 -0.8 2PUPPP 78.7 1.4 0.3JKG5E5 73.9 -3.4 -0.8 TSSRQR 78.7 1.4 0.32R20JW 74.0 -3.3 -0.8 BARYZ2 78.8 1.5 0.48II46Y 74.2 -3.1 -0.7 UYJBJJ 78.8 1.5 0.4

ZQHUPQ 74.2 -3.1 -0.7 G2GBDV 78.9 1.6 0.4LQ8989 74.3 -3.0 -0.7 G0B9NL 79.0 1.7 0.4RVP7VW 74.4 -2.9 -0.7 ZWL0DM 79.0 1.7 0.4VPHJRP 74.5 -2.8 -0.6 55BR5Z 79.1 1.8 0.4HJLSL6 74.8 -2.5 -0.6 WGZNNM 79.1 1.8 0.4GV2PGK 75.0 -2.3 -0.5 RJINCE 79.3 2.0 0.5OS838A 75.0 -2.3 -0.5 IQYQE4 79.3 2.0 0.5TI22ZK 75.0 -2.3 -0.5 LLL1RB 79.4 2.1 0.5

ODOOO2 75.1 -2.2 -0.5 GJIRPN 79.6 2.3 0.5E5LDLX 75.1 -2.2 -0.5 R7BBLB 79.6 2.3 0.5

MZMHCW 75.1 -2.2 -0.5 UUUOU9 79.7 2.4 0.5C9CDOR 75.2 -2.1 -0.5 HF3FYI 79.7 2.4 0.6211119.0 75.4 -1.9 -0.4 IMVADS 79.8 2.5 0.6JCUITV 75.6 -1.7 -0.4 CANEE8 80.0 2.7 0.6

SUUW3G 75.8 -1.5 -0.3 3PV3WL 80.1 2.8 0.7TZOONA 75.8 -1.5 -0.3 A8YSK1 80.1 2.8 0.7KK6WBZ 76.0 -1.3 -0.3 LNUUEI 80.1 2.8 0.7EHJ60F 76.3 -1.0 -0.2 OO9QOO 80.4 3.1 0.7

MDDETU 76.6 -0.7 -0.2 IWA0AA 80.5 3.2 0.7A5GR55 77.0 -0.3 -0.1 CZGHZZ 80.6 3.3 0.8

Code

Cocaine

Code

Cocaine

30





averagez-score

CU6JGV 80.8 3.5 0.8IXUAHB 81.0 3.7 0.9O4KFNX 81.0 3.7 0.9TQMVZE 81.3 4.0 0.9VBEY7Y 81.4 4.1 1.08J4DWT 82.0 4.7 1.1LL8LML 82.0 4.7 1.1FK5BHB 82.4 5.1 1.2CJWZIJ 83.0 5.7 1.3OTSOQ9 83.3 6.0 1.41YQDGQ 83.6 6.3 1.57JUSKK 83.9 6.6 1.58Q8VEF 84.0 6.7 1.6K3S33Y 84.9 7.6 1.8NZOTC6 87.0 9.7 2.2A7LUO7 87.5 10.2 2.4

MDQMM4 88.7 11.4 2.6Z8T8N1 89.0 11.7 2.79994MR 89.9 12.6 2.9WQOZYA 92.2 14.9 3.4H13HRX 93.4 16.1 3.7CWEOYI 93.8 16.5 3.810B0XJ 98.4 21.1 4.9

Cocaine

Code

31


Code Identified Substances0D11QZ JWH073

0HSVIE JWH-073

0LAKP0 JWH073

0P0W03 JWH - 073

10B0XJ not able to identify

1JJBJS JWH-073

1YQDGQ JWH-073

1ZKJHF JWH-073

211119 JWH-073

2PUPPP JWH-073

2R20JW JWH-073

33DUO3 Naphtalen-1-yl-(1-butylandol-3-yl) methanone (JWH-073)

3PV3WL JWH 073

40HFEP JWH-073

4J4H44 JWH-073

55BR5Z JWH073

5MLCMA JWH-073

5ZDDX6 JHW-073

7IUIII JWH-073

7JUSKK JWH-073

7T7YHK JWH-073

8AHAJA JWH-073

8D1PTX JWH-073, LACTOSE

8II46Y JWH-073

8J4DWT JWH-073

8MV6V6 JWH-073

8Q8VEF JWH-073

8WEYGQ JWH-073

8Z8ZZZ JWH-073

9994MR jwh-073

9Q999K JWH-O73

A33Q33 JWH-073

A5GR55 JWH-073

A7LUO7 JWH-073 AND LACTOSE

A8YSK1 JWH-073

AEDDRS JWH-073

APX4KD JWH-073

ARZMMQ JWH-073

ASLAA5 JWH-073

AYNT4F JWH-073

Sample 2 Analysis



32

Code Identified SubstancesB3SNNC JWH-073

BARYZ2 JWH-073

C9CDOR JWH-73

CANEE8 JWH 073

CCIVVK JWH-073

CJWZIJ JWH-073

CRSEZI JWH 073

CU6JGV WE CAN´T IDENTIFY THESE DRUGS

CWEOYI JWH-073

CZGHZZ JWH-073

DMM5Z5 JWH073

DWRNDI jwh-073

E2OA5N no investigado

E3MIHV JWH-073

E5LDLX JWH-073

E66LY6 JWH-073 and Lactose

EHJ60F JWH-073

EMJ8VM JWH 073

EXZYYP JWH-073

FK5BHB JWH-073

FMFLFF JWH073

G0B9NL 1-BUTYL-3-(1-NAPHTHOYL)INDOLE (JWH-073)

G2GBDV JWH-073

GGA8CG JWH-073

GJIRPN JWH-073

GKHDEM jwh-073

GNUILW JWH-073

GV2PGK JWH 073

HEHCMO JVH-073

HF3FYI JWH-073

HJLSL6 JWH-073

HRYNGY JWH-073

IMVADS JWH-073

IQYQE4 JWH-073

IWA0AA JWH-073

IXUAHB JWH-073

IZLLW0 JWH-073

JEVX45 jwh-073

JKG5E5 JWH-073

JM2GKM JWH-073

JSZ6XW JWH-073

K3S33Y JWH-073

KBUURH JWH-073

KKKWIC JWH-073



33

Code Identified SubstancesKSQKKK JWH-073

L4444V JWH-073(synthetic cannabinoid)

LDDCDD JWH

LL8LML JWH-073

LLL1RB JWH-073

LNUUEI JWH-073

LQ8989 JWH 073

LVVWC7 JWH-073

M1XUWT JWH-073

M72KOQ Cocaine

MCCCCC Napthalen-l-yl-(1-butylindol-3-yl) methanone (JWH-073)

MDDETU JWH-073

MDQMM4 JWH-073

MMMMT5 JWH-073

MOKSHS JWH-073

MWXNUW JWH-073 and Lactose

MZMHCW JWH-073

N8BTCF JWH-073

NWJDJI JWH-073

NZOTC6 JWH-073

O4KFNX JWH-073

O888ML JWH-073

ODOOO2 JWH073

OJFEOO Naphthalen-1-yl-(-butylindol-3-yl)methanone (JWH-073)

OK66R6 JWH-073

OO9QOO JWH-073

OQ4QQQ The sample number two was analyzed with GCMS and yielded one pik at Rt= 18.9( dihydromethyl dioxo...),which is not recognize/readable by the library.The library is installed in 2008.

OS838A JWH-073, lactose

OTSOQ9 JWH-073

PHWNRB JWH-073

PPLICW JWH-073

PQQKLQ NIL

R7BBLB JWH-073

RI3K33 Naphthalen-1 yl(1-butylindol-3-yl) methanone / JWH-073

RU8UQS jwh-073

SIFXH3 JWH-073

SMYNNU JWH-073

SUUW3G JWH-073

SVGG03 JWH-073

T2DI1R JWH-073

T4VMD2 JWH-073

TI22ZK JWH-073

TQMVZE JWH-073



34

Code Identified SubstancesTRVLKK JWH-073

TSSRQR JWH-073

TSW3IE JWH-073

TZOONA JWH-073

UFK6EF JWH-073

UG88A8 N/A

UJWYPY JWH-073

UMXXSY JWH-073

UUUOU9 Benzodiazepine (possible)

UWBOLU JWH-073

UXVVAC JWH-073

UYFCCR JWH-073

UYJBJJ JWH-073

V011CO JWH-073

VBEY7Y JWH-073

VFC8CC JWH-073

VIFKAW JWH-073

VPHJRP JWH 073

VVIRG4 JWH(073)

VVN4CQ JWH-073

W0HPES JWH73

WGZNNM JWH-073

WMTTV7 JWH-073

WQOZYA JWH-073

X0X444 JWH-073

XEE66N jwh-73

XNNNU1 JWH-073

XXFX8D JWH-073

YGQE84 JWH-073 (Naphtalen-1-y1-(1-butylindol-3-y1)methanone and Lactose

YLHIIH JWH-073

YQYLYY JWH-073

Z2BIDB JWH-073

Z8T8N1 JWH-073

ZCCQMZ JWH 073

ZQHUPQ JWH-073

ZWL0DM JWH073

ZXGCGG JWH-073



35

Code JWH-073 Lactose 0D11QZ + ANP

0HSVIE + ANP

0LAKP0 + -The sample contains JWH073 (not quantified).

0P0W03 + +A color test (SCOTT-Cobalt thiocyanate TEST) with positive reaction to Cocaine, to the TLC was used as a solvent for the preparation of the samples the Dichloromethane and as eluent methanol, used an aluminum plate 20 × 20 cm. Confirmation of results were made on the equipment of THERMO SCIENTIFIC NICOLET Is 10 and NICOLET Is 50 FT-IR.For 2016/2 sample/SM-2 should stress that the preliminary test was not conclusive. With the FT-IR sensed the presence of LACTOSE and other substances which are not suitable to specify here. With the TLC was able to identify the substance JWH-073.

10B0XJ - -Could not be identified

1JJBJS + +

1YQDGQ + +LACTOSE

1ZKJHF + ANPExamination revealed the presence of synthetic cannabinoids JWH-073

211119 + ANPResults of screening test were positive for amphetamines type stimulants. Relation time and fragmentation pattern obtained with sample are equivalent to those obtained with JWH-073.

2PUPPP + +Sample contains 14.39 ± 0.79 % (w/w) JWH-073. The sample also contains lactose.

2QC44H - ANP

2R20JW + ANPJWH-073 34.08%

33DUO3 + ANP

3PV3WL + +The sample SM2 contained JWH 073. Quantification of JWH 073 our institute not performed. Lactose was in the sample SM2 identify.

40HFEP + +Mass spectrometry profile correlation between sample and reference material certified

4J4H44 + -JWH-073 confirmed in 1 unit tested of 1 unit received.

55BR5Z + +./.

5MLCMA + ANPSample 2016/2/SM-2 found to contain JWH-073

5ZDDX6 + +the sample also contains lactosa

7666AG + ANPJWH-073




36

Code JWH-073 Lactose 7IUIII + -

The result of the sm 2 (117) tests received has confirmed that the mentioned simple contains JWH-073 but we have not been able to give a cuantification result.

7JUSKK + ANPIn Albanian is not in narcotic and psychotropic list

7T7YHK + +On analysis, I found sample SM-2 to contain JWH-073 and Lactose.

8AHAJA + +

8D1PTX + +

8II46Y + +JWH-073 was identified by GC-MS.

8J4DWT + ANP

8MV6V6 + ANPIn the test sample 2016/2/SM-2 was detected JWH-073

8Q8VEF + -

8WEYGQ + ANPWhite powder containing JWH-073.

8Z8ZZZ + +

9994MR + +

9Q999K + ANPsample SM-2 was found to contain JWH-073

A33Q33 + ANPJWH-073 confirmed

A5GR55 + +Lactose

A7LUO7 + +BY GC-MS AND FTIR

A8YSK1 + +JWH-073 and lactose

AEDDRS + ANP

APX4KD + +The white powder was analysed and found to contain (1-butyl-1H-indol-3-yl)(naphthalen-1-yl)methanone (JWH-073).

ARZMMQ + +additional substaces: lactose

ASLAA5 + +The sample was found to contain JWH-073 and lactose

AYNT4F + ANPDe análisis realizados se concluye que la sustancia se trata del cannabionide sintético identificado como JWH-073



37

Code JWH-073 Lactose B3SNNC + ANP

The sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule II 2. (2) (ii) A synthetic cannabinoid receptor type I against namely, 1-butyl-3-(1-naphthoyl)indole (JWH-073) or an isomer there of.

BARYZ2 + +

BFIU0G ANP ANPThe test sample number SM-2 is not delivered.

BRVRR4 - ANPExamination and analyses performed on SM2 did not reveal the presence of dangerous drugs.

C9CDOR + +

CANEE8 + +

CCIVVK + +The sample contain JWH-073.

CJWZIJ + +The sample contained 12.9% of JWH-073 and lactose.

CRSEZI + +lactose

CU6JGV - -

CWEOYI + +after chemical analysis was performed the sample was contain 2.566% JWH-073 & LACTOSE

CZGHZZ + +reference missing

DMM5Z5 + -Tested by GCMS

DNNSKS - -

DWRNDI + -jwh-073

E2OA5N ANP ANP

E3MIHV + ANP150: le Rf du spot donné par le standard est non identique avec le Rf obtenu avec la substance de référence JWH-073 (les deux uniquement, échantillon et standard ont été analysés par la suite sur la même plaque.

E5LDLX + +

E66LY6 + +

EHJ60F + -

EMJ8VM + +Sample 2016/2/SM-2 contains JWH 073 and lactose.

EXZYYP + ANPThe sample gave negative result with Marquis' and Cobalt Thiocyanate reagents. Confirmed by GC/MS, and quantified by GCFID, the sample contained JWH-073 16.8%.



38

Code JWH-073 Lactose FK5BHB + -

After analycing the simple with colorimetric tests, GC/MS confirmation we are able to say that this simple is JWH-073. It is not quantified because it does not have the righet stardands

FMFLFF + ANPJWH073, synthetic cannabinoid

G0B9NL + +White powder 228 milligrams 1 package 1-BUTYL-3-(1-NAPHTHOYL)-INDOLE (JWH-073) Class B

G2GBDV + ANPJWH-073 is controlled by K.B. of 22/01/1998, changed by K.B. of 26/09/2011

GGA8CG + +A sealed glass vial containing a white powder. This powder contained 1-BUTYL-3-(1-NAPHTHOYL)INDOLE (JWH-073).

GJIRPN + ANPJWH-073 is a Class B controlled drug subject to the provisions of the Misuse of Drugs Act 1971.

GKHDEM + ANPJWH-073 was detected by gc-ms screening and it was quantified by gc-fid using response factor

GNUILW + ANPJWH-073 belong to synthetic cannabinoid receptor agonists group, however, currently is not a controlled drug according to spanish legislation.

GUDEBT + ANPSample 2016/2/SM-2 a white powder gave a positive result for JWH-073.

GV2PGK + +JWH073 is present with lactose.

H13HRX - +LACTOSE

HEHCMO + +No

HF3FYI + ANPJWH-073, concentration approx 12%

HJLSL6 + +Sample contained JWH-073 and lactose. Identification of JWH 073 by FTIR and MS spectrum, quantification by GC/FID

HRYNGY + +POWDER

IMVADS + ANPSM-2 contains a drug: JWH-073, 13.8%

IQYQE4 + -

IRWMIX ANP +Sample 2016/2/SM-2 consists of white powder. No prohibited substances are detected in this sample.

IWA0AA + +The sample contains JWH-073 which is listed as a controlled drug according to french order, appendix IV of February 22nd 1990.

IXUAHB + +225 milligrams - JWH-073 - Class B Lactose - Not Controlled

IZLLW0 + ANPNon-controlled substance with effects similar to cannabis

JCUITV ANP ANP



39

Code JWH-073 Lactose JEVX45 + ANP

White powder containing jwh-073 not quantified

JKG5E5 + -JWH-073

JM2GKM + +Marquis, Scott y Simon'sGC-MS, FT-IR, UV

JSZ6XW + -The above article was examined and found to consist of a brown glass vial containing a quantity of white powder. The powder, which weighed 0.21 grams, was analysed and found to contain JWH-073.JWH-073 is a compound structurally derived from 3–(1–naphthoyl)indole by substitution at the nitrogen atom of the indole ring by an alkyl substituent, and as such is a class B controlled drug within the provisions of the Misuse of Drugs Act 1971.

K3S33Y + +The sample contains JWH-073. JWH-073 is a controlled substance in X.

KBUURH + +JWH 14.01 %, laktose

KK6WBZ + -

KKKWIC + ANPThe sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule II 2. (2) (ii) A synthetic cannabinoid receptor type I against namely, 1-butyl-3-(1-naphthoyl)indole (JWH-073) or an isomer there of.

KSQKKK + +JWH-073 was found in the sample

L4444V + +The sample was found to contain a SYNTHETIC CANNABINOID JWH-073(1-butyl-1H-indol-3YL)Naphtalen-1-YL, and LACTOSE

LDDCDD + ANPNo colour reaction with conventional reagents in our lab. But in TLC method (Toluene:Diethylamine system with Fast Blue B spray) Rf and colour of SM 2 is similar to Rf and colour of standard JWH. So SM 2 is JWH.

LL8LML + ANPJWH-073 was detected.

LLL1RB + +One glass bottle containing 0.23 grammes of a powder containing (1-butyl-1H-indol-3-yl)-1-naphthalenyl-methanone, being a compound structurally derived from 3-(1-naphthoyl)indole by substitution at the nitrogen atom of the indole ring with an alkyl substituent.

LNUUEI + +The powder is a misture of lactose and JWH-073, a synthetic cannabinoid. The concentration of JWH-073 in the sample is 13.2% by weight.

LQ8989 + ANPthe item is powder containing JWH073 non quantified

LVVWC7 + +The sample contains Lactose.

M1XUWT + -Powder (sample) contains JWH-073

M72KOQ - +Lactose



40

Code JWH-073 Lactose MCCCCC + ANP

From screening test using (MR) and ion scan and from confirmatory test using (GC-MS) the sample was found to contain the controlled drug JWH-073.

MDDETU + -Sample contains JWH-073

MDQMM4 + ANP

MMMMT5 + ANPThe mixture contains JWH-073 and some form of sugar

MOFYQQ - -negative

MOKSHS + -lactose

MWXNUW + +The Test sample 2016/2/SM-2 was analyzed by Colour test and Thin Layer Chromatography technique and foundto contain JWH-073 and Lactose.

MZMHCW + +It contains JWH-073. It also contains lactose

N8BTCF + +This sample contains JWH-073 and has lactose as diluent.

NWJDJI + +Based on the above analysis, the sample was found to contain JWH-073 and lactose.

NZOTC6 + ANP-

O4KFNX + +White powder containing 12.1%ww JWH-073 and lactose

O888ML + -The test sample 2016/2/SM-2 was found to contain the psychoactive substance Naphthalen-yl-(1-butylindol-3-yl) methanone (JWH-073).

ODOOO2 + +Sample also contain lactose. The presence of lactose and JWH073 were not as always confirmed by FTIR spectroscopy analysis because of equipment failure.

OJFEOO + -JWH-073 is included in Table I Schedule I drugs (narcotics-not use by medicine ) approved by Government Decision of Republic of Moldova no. 1088 of 05.10.2004 (Last Updated 5/13/2016) (tables and lists of narcotic, drugs, psychotropic substances and their precursors under control)

OK66R6 + +The sample also contains lactose.

OO9QOO + -lactose trace

OQ4QQQ - ANP

OS838A + +The sample contains JWH-073 which is scheduled as illicit drug in France. The lactose is not scheduled as illicit drug in France. It's a cutting agent.

OTSOQ9 + +cannabinoide sintetico

P0FZ8V - ANP



41

Code JWH-073 Lactose PHWNRB + +

The sample was found to contain JWH-073.

PPLICW + ANPThe sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule II 2. (2) (ii) A synthetic cannabinoid receptor type I against namely, 1-butyl-3-(1-naphthoyl)indole (JWH-073) or an isomer there of.

PQQKLQ ANP ANPTHE SAMPLE 2016/2/SM-2 WAS NOT INCLUDED IN THE ICE PACKAGE.

QQQQJW ANP ANP

R7BBLB + +JWH-073 (14.5 % w/w) and lactose

RI3K33 + +Further analysis revealed that percentage of JWH-073 in sample is 1.20. In addition to JWH -073, lactose was also identified in the sample by FTIR.

RJINCE + -JWH _073

RJNRSN + +JWH-073 and lactose were detected in sample 2016/2/SM-2. Quantitative analysis not performed.

RU8UQS + ANPwe have found results from swg-drug library, which the last version it, and we have studied for quantitative analysies with GC-FID.

RVP7VW ANP ANP

SIFXH3 + ANPSample 2016/2/SM-2 contains JWH-073.

SMYNNU + -The sample consist controlled drugs - JWH-073

SUUW3G + ANPontained JWH-073

SVGG03 + ANPThe sample contains 13.88 % JWH-073 .

T2DI1R + ANPThe chemical test and the coincidence in the retention time and mass fragmentogram with the reference material in the same conditions that the analyzed sample, indicate the JWH-073 presence.

T4VMD2 + ANPThis sample yield yellow color during colorimetric analysis been performed by using Froehde and Marquis reagent.Based on GC/MS analysis, JWH-073 had been identified

TI22ZK + ANPX grams of white powder containing JWH-073 (Naphthalen-1-yl(1-butylindol-3-yl)methanone. Class B

TQFFPF + ANPAnalyses performed on Item # 1 revealed presence of JWH-073 which is sintetic Canabinoid and which is not scheduled by the national Law on narcotics, psychotropic substances and precursors.

TQMVZE + +In the sample 2 we have faund JWH 073. JWH 073 is on the list of the drug in Montenegro.

TRVLKK + +SM2 contains JWH-073 and Lactose



42

Code JWH-073 Lactose TSSRQR + +

TSW3IE + +ANALYZED WHITE POWDER CONTAINS JWH-073

TZOONA + +JWH-073, LACTOSE

UFK6EF + +The sample contains JWH-073 and lactose.JWH-073 is sheduled as illicit drug.

UG88A8 ANP ANPN/A

UJWYPY + +lactose

UMXXSY + +JWH-073 - methanol extraction, alkaline solvent extraction (CHCl3) - GCMS - compare masspectra with library; Lactose - Extraction by ethanol - Residue - FTIR after evaporation to dryness under stream of Nitrogen - JWH-073 - compare IR spectra with library

UUUOU9 - -

UWBOLU + ANPthe white powder contains synthetic cannabinoid JWH-073 controlled by national law

UXVVAC + +Presence of JWH-073 and lactose was detected in the sample. The sample also contained multitude of trace level impurities.

UYFCCR + ANPIN SAMPLE 2016/2/SM-2 JWH-073 WAS FOUND.

UYJBJJ + +This sample contains JWH-073

V011CO + -The high quality of measurement results (99%) proves the presence of JWH-073 in the sample.

VBEY7Y + +Up to date it has never been detected in our laboratory.

VFC8CC + +

VIFKAW + +the sample contains naphthalen-1-yl-(1-butylindol-3-yl)methanone and lactose.

VPHJRP + +The sample containing 15.9% JWH073 and lactose.

VVIRG4 + +Sample found to contain JWH(073) and lactoseWe do not conduct quantitative drug samples JWH(073) lost by standard sample plos

VVN4CQ + +lactose

W0HPES + -base

WGZNNM + +

WMTTV7 + +The sample contains JWH-073, a sintetic cannabinoid and lactose



43

Code JWH-073 Lactose WQOZYA + ANP

X0X444 + +white powder containing JWH-073 and lactose as diluent

XEE66N + +the sample is containing 8.9% pure jwh-73

XNNNU1 + ANPPowder containing JWH-073Legal status: illegal - Portuguese NPS Law

XXFX8D + ANPThe sample found to contain JWH-073

YGQE84 + +Coloimetric reactions (CR) positive. GC/MS Retention time and mass charge (m/z) similar characteristics with the substances used as standard or pattern. FTIR absorption bands coincident with the substances used as pattern.

YLHIIH + -NAPHTHALEN-1-y1-(1butylindol-3-yl)methanone (JWH-073)

YQYLYY + ANPPresence of the synthetic cannabinoid JWH-073.

Z2BIDB + +On analysis, I found that the substances contain JWH-073.

Z8T8N1 + -

ZCCQMZ + +JWH 073 in lactose powder

ZQHUPQ + +It has been detected the presence of JWH-073 in the sample. This substance appears in the European Database on New Drugs since March 2009.

ZWL0DM + +LACTOSE

ZXGCGG + +The identification was realized with prelimimary color test with Marquis Reagent the confirmative assay with FT-IR and CG-MS and also the quantitative analysis.



44

Identification methods for Test Sample 2

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

0D11QZ SI I

0HSVIE SI

0LAKP0 S S S I I

0P0W03 S S I I

10B0XJ SI SI I SI Fast blue B salt

1JJBJS SI

1YQDGQ SI

1ZKJHF SI

211119 S S I

2PUPPP S I SI SI

2QC44H S S S I

2R20JW S S S I

33DUO3 SI I

3PV3WL SI I

40HFEP I I

4J4H44 I SI I

55BR5Z I SI SI

5MLCMA S I

5ZDDX6 S SI I I I I

7666AG I SI

7IUIII S S S I I

7JUSKK S S SI S fast blue B

7T7YHK S I I

8AHAJA S I I

8D1PTX SI SI I

8II46Y S S S I I

8J4DWT S I

8MV6V6 I

8Q8VEF I S

8WEYGQ S I SI

8Z8ZZZ S S I I

9994MR I SI

9Q999K S I

A33Q33 S S SI I

Analytical Technique Codes

Legend: S ... used for Screening I ... used for Identification SI ... used for both



45

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

A5GR55 SI I

A7LUO7 S S I I

A8YSK1 S I

AEDDRS SI

APX4KD S I I

ARZMMQ I SI

ASLAA5 S S I I

AYNT4F S S I I S Macroscopic

B3SNNC SI I

BARYZ2 SI I

BFIU0G S S I I

BRVRR4 S S S SI I I

C9CDOR S I

CANEE8 SI

CCIVVK S SI I SI

CJWZIJ SI I I UPLC-PDA

CRSEZI I I SI I

CU6JGV SI S SI I

CWEOYI I S I

CZGHZZ SI I

DMM5Z5 S I I

DNNSKS S I I I

DWRNDI I SI I

E2OA5N SI SI SI I

E3MIHV S S S I

E5LDLX I SI

E66LY6 S S S I I S ac. nitrico

EHJ60F S S S SI I SI

EMJ8VM S S S I I I Duquenois-Levine Test

EXZYYP S S S I I SI

FK5BHB S S I I Duquenois

FMFLFF SI I

G0B9NL S S S I I I

G2GBDV I S

GGA8CG S S I I S Basic Cobalt thiocynanate (for ketamine)

GJIRPN S S I

GKHDEM SI

GNUILW I SI

GUDEBT S S I I



46

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

GV2PGK I I

H13HRX SI SI S SI I I S Benedict

HEHCMO S S S SI I

HF3FYI SI I

HJLSL6 I I SI

HRYNGY SI

IMVADS S S S I I S

IQYQE4 I I

IRWMIX S S I I

IWA0AA I I

IXUAHB S S S I I

IZLLW0 S I S S I

JCUITV S S SI

JEVX45 I I SI

JKG5E5 SI S I SI

JM2GKM S S S I I I

JSZ6XW S I

K3S33Y I I

KBUURH SI I I

KK6WBZ I SI

KKKWIC SI I

KSQKKK S S I I I

L4444V S SI I

LDDCDD S S SI I

LL8LML S S I I

LLL1RB S S I SI I I Raman Spectroscopy

LNUUEI I I SI I

LQ8989 I

LVVWC7 S I SI I

M1XUWT I

M72KOQ I SI

MCCCCC S S I

MDDETU SI

MDQMM4 I SI

MMMMT5 S I I S

MOFYQQ S I I S Drug Testing Kits

MOKSHS I SI

MWXNUW

S S SI S Fast Blue B Salt Test

MZMHCW S I



47

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

N8BTCF I S I I

NWJDJI S I I

NZOTC6 SI

O4KFNX I I SI

O888ML S S S SI I

ODOOO2 S I I I

OJFEOO S S S I I I

OK66R6 S I SI I

OO9QOO SI SI

OQ4QQQ S S I SI

OS838A S S I

OTSOQ9 S I I S

P0FZ8V SI

PHWNRB SI

PPLICW SI I

PQQKLQ S I SI

QQQQJW I

R7BBLB I SI I I S Raman

RI3K33 S S I I I S Fast Blue B salt test

RJINCE S S I I

RJNRSN S SI I

RU8UQS S S I SI

RVP7VW SI SI SI I

SIFXH3 S S S I I

SMYNNU I SI I

SUUW3G SI

SVGG03 SI I

T2DI1R S S I

T4VMD2 S I

TI22ZK S S I

TQFFPF S S I I

TQMVZE S S I I I

TRVLKK I S

TSSRQR SI I

TSW3IE S S I I

TZOONA S S S I I I I XRD

UFK6EF I SI I

UG88A8 SI S SI SI S Simon

UJWYPY S SI SI I



48

Code 140 141 142 150 160 161 170 171 172 180 190 200 211 220 230 231 210

UMXXSY S S SI I

UUUOU9 SI SI SI I

UWBOLU I SI

UXVVAC S I I I I

UYFCCR I SI

UYJBJJ S I I

V011CO S S I I

VBEY7Y SI S S I I I

VFC8CC SI

VIFKAW SI I

VPHJRP S I I I I HPLC/RID

VVIRG4 S I I I LC-MS-TOF

VVN4CQ I SI SI

W0HPES I SI I

WGZNNM I SI

WMTTV7 SI SI

WQOZYA SI I

X0X444 SI I

XEE66N SI S S SI I

XNNNU1 S S I

XXFX8D S S I S Janovsky

YGQE84 S S S I I

YLHIIH I SI

YQYLYY I

Z2BIDB S S S I I I Raman Spectrometer

Z8T8N1 SI I

ZCCQMZ I SI I

ZQHUPQ I S I

ZWL0DM SI I

ZXGCGG S S S I I



49

Theof s

RouCP

O1HBM2DMU

Freq

RespPartTecScre

Iden

Qua

e response screening an

und Code: CU6JGV P0FZ8V Q4QQQ

10B0XJ H13HRX

RVRR4 OFYQQ QC44H NNSKS

M72KOQ UUOU9

quency of

sponse Summticipants 181hniqueeening (No

(%

tification (No

(%

antification (No(%

summary ofnd identifica

SubstaJWH-0Lactos

RoM7UUXN

False

Answe-----------

use of scre

mary

140 141o.) 54 75

%) 29.8 41.4

o.) 13 6

%) 7.2 3.3

o.) 0 0%) 0.0 0.0

RESPON

f all 181 paation techniq

ance Name 073 se

F

und Code: 72KOQ UUOU9

NNU1

negatives

r Code: - - - - - - - - - - -

eening, ide

1 142 1505 62 19

4 34.3 10.5

5 34

3 2.8 18.8

0 00 0.0 0.0

NSE SUMM

Par

rticipating laques and fa

Identificati

Num

alse positi

SubstancCocaineBenzodiaMorphine

for Test Sa

Tech Cod171

180/180180/180

180 150/180

180 161/180

150 150/180

180 180

entification

0 160 1610 3

5 0.0 1.7

5 12

8 2.8 6.6

8 246.2 18.6

MARY FOR

rticipants:

aboratories alse positive

ion of Test

mber of labo

ves for Tes

ce reported

azepine (poe

ample-2 (te

de:

0 0

0

0

0

R

n and quant

170 1710 4

0.0 2.2

1 35

0.6 19.3

0 766 0.0 58.9

R TEST SA

181

for the idenes and nega

t Sample-2

oratories cor162 89

st Sample-

: Te18

ossible) 1418

echniques(s

Round CodeQQQQJWRVP7VWJCUITV E2OA5NIRWMIX PQQKLQBFIU0G UG88A8

tification te

172 1800 72

0.0 39.8

1 168

0.6 92.8

0 300.0 23.3

AMPLE 2

ntification ofatives:

:

rrectly ident

2:

ech Code: 80 40/171 80

s) used for

e: Answ

echniques:

190 20019 1

10.5 0.6

79 4

43.6 2.2

0 00.0 0.0

f SM-2, freq

tifying:

r ANP):

wer Code: ANP ANP ANP ANP ANP ANP ANP ANP

:

210 21113 0

7.2 0.0

8 3

4.4 1.7

1 30.8 2.3

quency of u

Tech C--------

220 2301 2

0.6 1.1

0 7

0.0 3.9

0 10.0 0.8

se

ode:

2310

0.0

4

2.2

00.0

2016/2-SM Copyright (c) 2017 UNODC50


Robust average = 13.2 %

Round: 2016/2 UNODC value = 14.5 %Substance: SM‐2; JWH‐073

‐5.4

‐2.2 ‐2.1 ‐2.0

‐1.6

‐1.6 ‐1.5

‐1.2 ‐1.1 ‐1.0

‐0.9

‐0.6 ‐0.4 ‐0.4

‐0.2

‐0.1 ‐0.1

‐0.1

0.0 0.1 0.1

0.1 0.3

0.3 0.3 0.4 0.4 0.5 0.6 0.6

0.6 0.7

0.7 0.8

1.3 1.4

1.8 1.8

3.0

10.6

‐4.00

‐3.00

‐2.00

‐1.00

0.00

1.00

2.00

3.00

4.00

CWEO

YI

XEE66N

Z8T8N1

LVVW

C7

MOKSHS

TSSRQR

W0H

PES

MDQ

MM4

ZWL0DM

SMYN

NU

5ZDD

X6

KK6W

BZ

WQOZYA

HF3FYI

NZO

TC6

CJWZIJ

4J4H

44

A5GR

55

LNUUEI

CRSEZI

HEHC

MO

SUUW3G

8II46Y

33DU

O3

IMVA

DS

SVGG

03

KBUURH

O4K

FNX

UXV

VAC

2PUPP

P

1JJBJS

R7BB

LB

7666

AG

HJLSL6

GKHD

EM

VPHJRP

VFC8

CC

EXZYYP

ZXGC

GG

2R20JW

z‐scores can be interpreted by participants in line with ISO 13528:2005, secion 7.4.2 and ISO/IEC guide43‐1:1997(E) as follows:IzI 3 = unsatisfactory

According to the recommendations in ISO 13528:2005, an unsatisfactory z‐score is considered to give an action signal and a questionable z‐score is considered to give a warning signal. A single action signal or warning signal in two successive rounds shall be taken that an anomaly has occured that requires inverstigation.

51


Round: 2016/2Substance: SM‐2; JWH‐073

-10

-8

-6

-4

-2

0

2

4

6

8

10

z-sc

ore

Dispersion of z-scores using different analytical techniques for the quantification of JWH-073 in 2016/2 SM-2

HPLC GC‐FID GC‐MS NMR

52

Z-Scores for Test Sample 2





averagez-score

CWEOYI 2.6 -10.6 -5.4XEE66N 8.9 -4.3 -2.2Z8T8N1 9.0 -4.2 -2.1LVVWC7 9.2 -4.0 -2.0MOKSHS 10.0 -3.2 -1.6TSSRQR 10.0 -3.2 -1.6W0HPES 10.3 -2.9 -1.5MDQMM4 10.9 -2.3 -1.2ZWL0DM 11.0 -2.2 -1.1SMYNNU 11.3 -1.9 -1.05ZDDX6 11.3 -1.9 -0.9KK6WBZ 12.0 -1.2 -0.6WQOZYA 12.3 -0.9 -0.4HF3FYI 12.4 -0.8 -0.4NZOTC6 12.9 -0.3 -0.2CJWZIJ 12.9 -0.3 -0.14J4H44 13.0 -0.2 -0.1A5GR55 13.0 -0.2 -0.1LNUUEI 13.2 0.0 0.0CRSEZI 13.3 0.1 0.1

HEHCMO 13.4 0.2 0.1SUUW3G 13.4 0.2 0.18II46Y 13.7 0.5 0.3

33DUO3 13.7 0.5 0.3IMVADS 13.8 0.6 0.3SVGG03 13.9 0.7 0.4KBUURH 14.0 0.8 0.4O4KFNX 14.1 0.9 0.5UXVVAC 14.3 1.1 0.62PUPPP 14.4 1.2 0.61JJBJS 14.4 1.2 0.6R7BBLB 14.5 1.3 0.77666AG 14.5 1.3 0.7HJLSL6 14.8 1.6 0.8

GKHDEM 15.7 2.5 1.3VPHJRP 15.9 2.7 1.4VFC8CC 16.7 3.5 1.8EXZYYP 16.8 3.6 1.8ZXGCGG 19.1 5.9 3.02R20JW 34.1 20.9 10.6

Code

JWH-073

53


Code Identified Substances0D11QZ ketamine

0HSVIE Ketamine

0LAKP0 Ketamine

0P0W03 KETAMINE

10B0XJ Ketamine

1JJBJS ketamine

1YQDGQ KETAMINE HCL

1ZKJHF Ketamine

211119 Ketamine

2PUPPP Ketamine

2QC44H Ketamine

2R20JW ketamine

33DUO3 Ketamin

3PV3WL Ketamine

40HFEP Ketamine

4J4H44 Ketamine hydrochloride

55BR5Z KETAMINE

5MLCMA Ketamine

5ZDDX6 ketamine

7666AG ketamine

7IUIII KETAMINE

7JUSKK ketamine

7T7YHK Ketamine

8AHAJA ketamine

8D1PTX KETAMINE, LACTOSE

8II46Y Ketamine

8J4DWT ketamine

8MV6V6 ketamine

8Q8VEF Ketamine

8WEYGQ Ketamine

8Z8ZZZ ketamine

9994MR ketamine

9Q999K ketamine

A33Q33 Ketamine

A5GR55 Ketamine

A7LUO7 KETAMINE AND LACTOSE

A8YSK1 Ketamine

AEDDRS ketamine

APX4KD Ketamine

ARZMMQ ketamine

Sample 3 Analysis



54

Code Identified SubstancesASLAA5 Ketamine

AYNT4F Ketamina

B3SNNC Ketamine

BARYZ2 ketamine

BFIU0G ketamine

C9CDOR ketamine + lactose

CANEE8 Ketamin

CCIVVK ketamine

CJWZIJ Ketamine

CRSEZI ketamine

CU6JGV KETAMINE

CWEOYI ketamine

CZGHZZ kétamine

DMM5Z5 Ketamine

DNNSKS Ketamine

DWRNDI ketamine

E2OA5N ketamine

E5LDLX ketamine

E66LY6 Ketamine and Lactose

EHJ60F KETAMINE

EMJ8VM ketamine

EXZYYP Ketamine HCl

FK5BHB Ketamine

FMFLFF ketamine

G0B9NL KETAMINE HYDROCHLORIDE

G2GBDV ketamine

GGA8CG Ketamine Hydrochloride

GJIRPN Ketamine

GKHDEM ketamine

GNUILW Ketamine

GUDEBT KETAMINE

GV2PGK ketamine hydrochloride

H13HRX COCAINE, KETAMINE

HEHCMO Ketamine

HF3FYI ketamine

HJLSL6 Ketamine

HRYNGY KETAMINE

IMVADS Ketamine

IQYQE4 Ketamine

IRWMIX Ketamine

IWA0AA Ketamine

IXUAHB Ketamine

IZLLW0 Ketamine

JCUITV KETAMINE



55

Code Identified SubstancesJEVX45 ketamine

JKG5E5 ketamine

JSZ6XW Ketamine

KBUURH ketamine hydrochloride

KKKWIC ketamine

KSQKKK Ketamine

L4444V KETAMINE (Anesthetic)

LDDCDD Ketamine

LL8LML Ketamine

LLL1RB Ketamine

LNUUEI ketamine

LQ8989 Ketamine Hydrochloride

LVVWC7

M1XUWT Ketamine

M72KOQ Ketamine

MCCCCC Ketamine

MDDETU Ketamine

MDQMM4 Ketamine

MMMMT5 ketamine

MOFYQQ ketamine

MOKSHS Ketamine

MWXNUW Ketamine and Lactose

N8BTCF Ketamine

NWJDJI Ketamine

NZOTC6 Ketamine

O4KFNX ketamine

O888ML Ketamine

ODOOO2 Ketamine

OJFEOO Ketamine

OK66R6 ketamine

OO9QOO Kétamine

OQ4QQQ ketamin

OS838A ketamine, lactose

OTSOQ9 KETAMINA

P0FZ8V ketamine

PHWNRB Ketamine

PPLICW Ketamine

PQQKLQ KETAMINE

QQQQJW ketamine

R7BBLB Ketamine

RI3K33 Ketamine

RJINCE Ketamine

RJNRSN Ketamine

RU8UQS ketamine



56

Code Identified SubstancesRVP7VW ketamine

SIFXH3 Ketamine

SMYNNU Ketamine

SUUW3G Ketamine

SVGG03 Ketamine

T2DI1R Ketamine

T4VMD2 Ketamine

TI22ZK Ketamine

TQMVZE Ketamine

TRVLKK Ketamine

TSW3IE KETAMINE

TZOONA KETAMINE hcl

UFK6EF Ketamine

UG88A8 KETAMINE

UJWYPY ketamine

UMXXSY Ketamine

UUUOU9 ketamine

UWBOLU ketamine

UXVVAC Ketamine

UYFCCR KETAMINE

UYJBJJ Ketamine

V011CO Ketamine

VBEY7Y Ketamine

VFC8CC Ketamine

VIFKAW Ketamine

VPHJRP Ketamine

VVIRG4 Ketamine

VVN4CQ ketamine

W0HPES ketamine

WGZNNM Ketamine

WMTTV7 Ketamine

WQOZYA ketamine

X0X444 ketamine

XEE66N ketamin

XNNNU1 Ketamine

XXFX8D Ketamine

YGQE84 Ketamine

YLHIIH KETAMINE

YQYLYY katmine

Z2BIDB ketamine

Z8T8N1 ketamine

ZCCQMZ Ketamine

ZQHUPQ ketamine hydrochloride

ZWL0DM KETAMINE



57

Code Identified SubstancesZXGCGG Ketamine



58

Code Ketamine Lactose 0D11QZ + ANP

0HSVIE + ANP

0LAKP0 + -The sample contains ketamine.

0P0W03 + +A color test (SCOTT-Cobalt thiocyanate TEST) with positive reaction to Cocaine, to the TLC was used as a solvent for the preparation of the samples the Dichloromethane and as eluent methanol, used an aluminum plate 20 × 20 cm. Confirmation of results were made on the equipment of THERMO SCIENTIFIC NICOLET Is 10 and NICOLET Is 50 FT-IR.For 2016/2 sample/SM-2 should stress that the preliminary test was not conclusive. With the FT-IR sensed the presence of LACTOSE and other substances which are not suitable to specify here. With the TLC was able to identify the substance KETAMINE.

10B0XJ + -Confirmatory test (GCMS) shows test sample contains ketamine

1JJBJS + +

1YQDGQ + +LACTOSE

1ZKJHF + ANPExamination revealed the presence of Ketamine

211119 + ANPResults of screening test were positive for cocaine. Relation time and fragmentation pattern obtained with sample are equivalent to those obtained with ketamine.

2PUPPP + +Sample contains 10.56 ± 0.59 % (w/w) Ketamine base. The sample also contains lactose.

2QC44H + ANPThe sample obtains with positive result with Marquis reagent.TLC : The Rf sample obtain with the same Rf Ketamine Standard.GC : The Rt sample obtain with the same Rt Ketamine Standard.

2R20JW + ANPketamine chloridrate 10,49% as base

33DUO3 + ANP

3PV3WL + +The sample SM3 contained 10,1 % of ketamine. Lactose was in this sample identify.

40HFEP + +Mass spectrometry profile correlation between sample and reference material certified

4J4H44 + +Ketamine hydrochloride and lactose confirmed in 1 unit tested of 1 unit received.

55BR5Z + +./.

5MLCMA + ANPSample 2016/2/SM-3 found to contain Ketamine

5ZDDX6 + +the sample also contains lactosa

7666AG + ANP




59

Code Ketamine Lactose 7IUIII + -

The result of the sm 3 (25) tests received has confirmed that the mentioned simple contains ketamine but we have not been able to give a cuantification result.

7JUSKK + ANP

7T7YHK + +On analysis, I found sample SM-3 to contain Ketamine and Lactose.

8AHAJA + +

8D1PTX + +

8II46Y + +Ketamine was identified by GC-MS.

8J4DWT + ANP

8MV6V6 + ANPIn the test sample 2016/2/SM-3 was detected ketamine

8Q8VEF + -

8WEYGQ + ANPWhite powder containing ketamine.

8Z8ZZZ + +

9994MR + +White powder, totally soluble in water, slightly soluble in chloroform

9Q999K + ANPsample SM-3 was found to contain 11.4% of ketamine.

A33Q33 + ANPKetamine confirmed

A5GR55 + +Lactose

A7LUO7 + +BY GC-MS AND FTIR

A8YSK1 + +Ketamine and lactose

AEDDRS + ANP

APX4KD + +The white powder was analysed and found to contain ketamine.

ARZMMQ + +additional substaces: lactose

ASLAA5 + +The sample was found to contain ketamine and lactose

AYNT4F + ANPDe análisis realizados se concluye que la sustancia se trata de ketamina

B3SNNC + ANPThe sample was found to contain a controlled substance within the meaning of the Controlled Drug and Substances Act: Schedule I 14. (1) Ketamine (2-(2-chlorophenyl)-2-(methylamino)cyclohexanone)

2016/2-SM Copyri

international quality assurance programme ......the ice programme is a unodc mandated activity and...

Documents