improving rast annotations within rast
DESCRIPTION
Improving RAST annotations within RAST. Veronika Vonstein Fellowship for Interpretation of Genomes December 2008. Your genome after RAST. SEED genome in RAST. Use Function based c omparison t ool. Choose genome to compare to. - PowerPoint PPT PresentationTRANSCRIPT
www.rast.nmpdr.org
Improving RAST annotations within RAST
Veronika VonsteinFellowship for Interpretation of Genomes
December 2008
www.rast.nmpdr.org
Your genome after RAST
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SEED genome in RAST
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Use Function based comparison tool
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Choose genome to compare to
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Compare Metabolic Reconstruction of your genome (A) and the SEED
genome (B)
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1. Case: Candidate present but Function different
But to eliminate the risk of annotating a paralog
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Compare Regions
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Annotate Chromosomal Clusters
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Annotate set 1
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Set 1 done continue
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2. Case: The real missing
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3. Case: Missed gene call
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Your genome after RAST
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Your improved genome
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Summary
• Re-annotated 490 gene functions• Increased the number of pegs in
subsystems from 36% to 50%• This added 60 subsystems to the
metabolic reconstruction of your genome• Possible to add missed genes, annotate
them and connect them to subsystems