OOOO ABSTRACTS

Volume 117, Number 2 Abstracts e223

Oleate monoethanolamine offers effective and safe treatment.This study shows the results of treatment of BVLs withsclerotherapy using 5% ethanolamine oleate. The recordsof patients treated at School of Dentistry of UFMG forBVL were reviewed retrospectively, noting resolution, post-operative complications, and degree of satisfaction withtreatment. The sample consisted of 15 patients (19 BVLs), 7(46.7%) men and 8 (53.3%) women, with a mean age of 62.3years. Resolution of lesions was observed an average of 14days after initiation of treatment. There were no reportedpostoperative complications, and patients’ level of satisfactionwas above 8 on a visual analog scale. Our results suggest thatsclerotherapy with 5% ethanolamine oleate can be an effectivetreatment for BVL. CNPq #309209/2010-2; 472045/2011-3,FAPEMIG.

PE-463 - SCROFULA: A PUBLIC HEALTH PROBLEM.ANNA TEREZA OLIVEIRA GÓES SIQUEIRA CAMPOSLIMA, JOÃO DE JESUS VIANA PINHEIRO, ANDRÉ LUISRIBEIRO RIBEIRO, SERGIO DE MELO ALVES JUNIOR.UNIVERSIDADE FEDERAL DO PARÁ.

Tuberculosis (TB) is a contagious infectious disease, com-mon in developing countries, where the health strategies are lowand weak. Therefore special care is needed on the part of healthprofessionals and the population because there is no predictableway to eradicate TB. Usually its manifestation is pulmonary, butin a few cases it can be extrapulmonary, as in skeletal, urogenital,and lymph node tuberculosis. Brazilian young woman, 18, cameto the CESUPA’s pathology department complaining of pain andswelling of the left submandibular cervical region. The standardsubmandibular incision was used to excise the enlarged lymphnode and specimens were sent for histological evaluation andculture. Tissue cultures were positive for Mycobacterium tuber-culosis. Final histological results revealed necrotizing, granulo-matous, and multinucleated giant cells. The diagnosis was basedon the lymph node histological culture, permitting appropriatetreatment.

PE-464 - SEVERE SECONDARY HYPERPARATHYROID-ISM AND PANORAMIC RADIOGRAPHY PARAMETERS.JOÃO CÉSAR GUIMARÃES HENRIQUES, REINHILDEJACOBS, RAFAELA RANGEL ROSA, SÉRGIO VITORINOCARDOSO, CAIO VINÍCIUS BARDI MATAI, JULIOCEZAR DE MELO CASTILHO. UNIVERSIDADE FED-ERAL DE UBERLÂNDIA.

This study evaluated the effects of renal osteodystrophy(RO) using panoramic radiography parameters. Materials andMethods: Qualitative (mandibular cortical index/trabecular bonepattern) and quantitative (mental index/calcification andresorption foci) parameters were applied from panoramic ra-diographs of chronic kidney disease (CKD) patients and con-trols. Results: Qualitative parameters demonstrated significantdifferences compared to the control group. The mean mentalindex was lower in patients with CKD, but the difference wasnot significant. Parathyroid hormone levels correlated withmental index, mandibular cortical index, and trabecular bonepattern. The mental index, mandibular cortical index, andtrabecular bone pattern are good parameters for evaluating theeffects of renal osteodystrophy. Conclusion: Renal osteodys-trophy presented sufficient characteristics that could be identi-fied by panoramic radiographic parameters.

PE-465 - SHOULD PERICORONAL FOLLICLES ASSO-CIATED WITH MANDIBULAR THIRD MOLARSROUTINELY BE SENT FOR HISTOPATHOLOGICALANALYSIS? A PROSPECTIVE STUDY AND SYSTEMATICREVIEW OF THE LITERATURE. THALES SALLESANGELIM VIANA, GALYLÉIA MENESES CAVALCANTE,PAULO GOBERLÂNIO BARROS SILVA, ROBERTABARROSO CAVALCANTE, ALEXANDRE SIMÕESNOGUEIRA, KARUZA MARIA ALVES PEREIRA, FÁBIOWILDSON GURGEL COSTA. UNIVERSIDADE FEDERALDO CEARÁ.

Pathological changes associated with third molar follicleshave been used to justify prophylactic tooth removal. This studyinvestigated the pathological alterations related to mandibularthird molar dental follicles. A prospective study was conductedwith 120 patients. Patients were divided into two groups: G1(presence of lesion) and G2 (absence of lesion). A systematicreview of the literature was also performed. A total of 113 sur-gical specimens were analyzed. G1 was the most prevalentfinding (p ¼ 0.0004). Lesions were found in patients betweenages 20 and 25 years (p < 0.004). The most prevalent histologicaldiagnosis was paradental cyst (47.7%; p < 0.0001). The sys-tematic review of the literature showed that the majority of casesoccur in patients under age 25 years and are mainly dentigerouscysts (p < 0.05). According to this study, prophylactic removal ofmandibular third molars is suggested, in addition to routinelysending pericoronal follicles for histopathological analysis.

PE-466 - SPLUNC2A EXPRESSION IN PATIENTSHAVING AUTOLOGOUS BONE MARROW TRANS-PLANTATION. LARA MARIA ALENCAR RAMOS,WILFREDO ALEJANDRO GONZÁLEZ-ARRIAGADA,ANDRÉIA APARECIDA DA SILVA, MARIA ELVIRAPIZZIGATTI CORREA, LYNNE BINGLE, PABLO AGUSTINVARGAS, MARCIO AJUDARTE LOPES. PIRACICABADENTAL SCHOOL, UNIVERSITY OF CAMPINAS, UNI-CAMP, SÃO PAULO, BRAZIL.

This study analyzed SPLUNC2A expression in patients whounderwent high-dose chemotherapy for autologous hematopoieticstem cell transplantation (AHSCT). Materials and Methods: Astudy was performed with 14 AHPCT patients (mean age 45years). Unstimulated saliva was collected for 5 min before thetransplant, as well as 7 (D+7) and 21 (D+21) after. SPLUNC2Aexpression was analyzed by Western blotting. Data were analyzedstatistically (ANOVA, T test, p < 0.05). Results: Densitometry ofprotein bands showed a higher level of SPLUN2A after trans-plant, measuring 0.54 � 0.22 compared to 0.44 � 0.35 before thetransplant (p ¼ 0.58). In addition, salivary flow rate decreasedafter AHSCT, measuring 0.40 � 0.33 compared with 0.47 � 0.31before the AHSCT (p ¼ 0.55). Conclusion: The present study,despite the small sample, describes recent findings showing thatpatients who undergo AHSCT may have changes in SPLUNC2Aexpression and in salivary flow rate.

PE-467 - IMMUNE CELLS IN DIFFERENT CLINICALPRESENTATIONS OF DENTURE STOMATITIS. KARENHENRIETTE PINKE, PATRÍCIA FREITAS-FARIA, THAÍSHELENA GASPAROTO, ALINE CARVALHO BATISTA,TAIANE PRISCILA GARDIZANI, VANESSA SOARESLARA. BAURU SCHOOL OF DENTISTRY, UNIVERSITYOF SÃO PAULO.

ORAL AND MAXILLOFACIAL PATHOLOGY OOOO

e224 Abstracts February 2014

Denture stomatitis (DS) is a frequent lesion that affects themucosa covered by a denture, especially in elderly patients. Theetiology is multifactorial, but is strongly associated with Candidaalbicans. We quantified different cells in DS lesions clinicallyclassified as simple or diffuse inflamed mucosa (DS1) or hyper-plastic surface (DS2) under a maxillary complete denture(papillary hyperplasia). The molecules CD3, CD4, CD8, CD20,CD68, and mast cell tryptase were labeled by immunohisto-chemistry in 32 DS cases and 6 healthy human palatal mucosasamples (control) obtained from the files of the Anatomical Pa-thology Laboratory of FOB/USP. DS1 lesions demonstrate acellular infiltrate similar to that of controls. However, in the DS2group, CD4 and CD8 lymphocytes, mast cells, and B cells weremore prevalent in connective tissue. These changes may indicatean association between aspects of immune response and differentclinical presentations of DS.

PE-468 - INDUCTION OF APOPTOSIS IN DENTURESTOMATITIS BY CANDIDA ALBICANS. ANA REGINACASAROTO, RAFAELA ALVES DA SILVA ALAVARCE,SAMIRA SALMERON, MARIA FÁTIMA GUARIZOKLINGBEIL, MONICA BEATRIZ MATHOR, MARIALÚCIA RUBO DE REZENDE, VANESSA SOARES LARA.BAURU SCHOOL OF DENTISTRY, UNIVERSITY OF SÃOPAULO.

The presence of Candida albicans in adherent biofilmunder prostheses is related to denture stomatitis (DS). Thisstudy evaluated the ability of C. albicans to induce apoptoticevents in human epithelium cells in vitro. Study Design: Cellsobtained from palatal tissue were challenged by direct andindirect contact with viable C. albicans (0.01/1, 0.025/1, and0.1/1 yeast/keratinocyte) at 3, 6, and 10 hours. Apoptotic cellswere determined by fluorescent nuclear staining, expressed as apercentage of the total number of cells counted, and evaluatedusing contrast and/or Mann-Whitney tests (p < 0.05). Results:In the direct assay, the ratio of 0.025/1 caused significantapoptosis at the 3-hour assessment compared with other times.The increase of the fungal concentration resulted in significantcell apoptosis compared to unchallenged epithelium. The samewas observed for the indirect assay. Conclusion: These resultssuggested that C. albicans induces early epithelial apoptosis inthe DS.

PE-469 - MDM2 AND SUMO-1 EXPRESSION INACTINIC CHEILITIS AND LIP CANCER. ADRIANA DAMOTA DELGADO, MÔNICA GHISLAINE OLIVEIRAALVES, TABATA DE MELO TERA, IVAN BALDUCCI,YASMIN RODARTE CARVALHO, ANA SUELI RODRIGUESCAVALCANTE, JANETE DIAS ALMEIDA. UNIVERSIDADEESTADUAL PAULISTAeUNESP.

This study compared the expression of MDM2 and SUMO-1proteins between actinic cheilitis and squamous cell carcinoma(SCC) of the lip. Methods: The sample consisted of lower lipmucosa specimens obtained from patients with a clinical andhistopathological diagnosis of actinic cheilitis (n ¼ 26) and SCC(n ¼ 25) as well as specimens of semimucosa (n ¼ 15) withoutclinical alterations. The tissue samples were stained with hema-toxylin-eosin and anti-MDM2 and anti-SUMO-1 antibodies.Data were analyzed by Kruskal-Wallis and Dunn’s tests (5%).Results: The median expression of MDM2 and SUMO-1 wassimilar in cases of actinic cheilitis and SCC of the lip, butdiffered significantly from that observed for semimucosa

specimens. Conclusion: We observed an overexpression ofMDM2 and SUMO-1 in cases of actinic cheilitis and lip SCC, sowe believe that it could be related to the regulatory mechanismsof apoptosis.

PE-470 - CONNECTION BETWEEN MONONUCLEARCELL INFILTRATE AND THE EXPRESSION OF KI-67,COLLAGEN TYPE IV, AND LAMININ IN RADICULARCYSTS. RENATA VERAS CARVALHO MOURÃO, ANAPAULA NEGREIROS NUNES ALVES, ALCEU MACHADODE SOUSA, DENNYS RAMON DE MELO FERNANDESALMEIDA, ANA LARIYSSA FERREIRA GOMES PORTO,MÁRIO ROGÉRIO LIMA MOTA, ERASMO BERNARDOMARINHO. UNIVERSIDADE FEDERAL DO CEARÁ.

Radicular cyst (RC) and dentigerous cyst (DC) have differentetiopathogenesis. This study evaluated the relationship betweeninflammatory infiltrate, expression of proliferative immuno-markers, proteins of basement membrane (BM), and extracellularmatrix in RC. The expression of Ki-67, collagen type IV (C-IV),and laminin were examined in highly inflamed RC (HIRC)(n¼17), slightly inflamed RC (SIRC) (n¼9), and DC (n¼9). Theimmunoexpression of Ki-67 was higher in SIRC than in HIRCand DC. The immunoexpression of C-IV in the BM of the SIRCwas lower than in HIRC and DC. The anti-laminin expression inBM of HIRC and DC was negative, and in SIRC it was focal. Itwas concluded that severity of inflammatory content in the RCwall appeared to modify the expression of proliferation factors inthe BM, but not in the extracellular matrix.

PE-471 - SURVIVAL ANALYSIS AND FOLLOW-UPOF MALIGNANT ODONTOGENIC TUMORS. THAISSOUSA DA SILVA, DANIELA OTERO PEREIRA COSTA,PAULO ANTÔNIO FARIA, DANIELLE RESENDECAMISASCA, FERNANDO LUIZ DIAS, SIMONELOURENÇO, VANESSA SOARES LARA. INSTITUTONACIONAL DO CÂNCER (INCA) - RJ, BRASIL.

This study presents the clinicopathologic features, survival,and follow-up of a series of malignant odontogenic tumors.Methods: Fourteen cases of malignant odontogenic tumors werereviewed. Sociodemographic and clinicopathologic data wererecorded. Survival was analyzed using the Kaplan-Meier method.Results: The most common tumor was ameloblastic carcinoma(50.0%), followed by malignant odontogenic tumors not other-wise classified (21.43%). The average age was 46.2 years old, themale to female ratio was 1.6:1, and the maxilla was the site mostoften affected (64.28%). Surgical resection was the treatmentemployed in all cases. Mean overall survival (OS) and disease-free interval were 73.88 months and 54.93 months, respectively.Survival analysis identified maxilla (p ¼ 0.05) and diagnosis (p ¼0.006) as being associated with the worst OS. Conclusions: Theseneoplasms present an aggressive and sometimes lethal course,especially tumors in the maxilla, which was the most meaningfulparameter in survival analysis.

PE-472 - SYNDECAN-1 EXPRESSION IN SOLID AME-LOBLASTOMA AND CALCIFYING CYSTIC ODONTO-GENIC TUMOR. SABRINA NOGUEIRA DE MORAES,FABIO RAMOA PIRES, LUCIANA ARMADA DIAS,REBECA SOUZA AZEVEDO. FACULDADE DE ODONTO-LOGIA DE PIRACICABAeUNICAMP.

Ameloblastoma (AME) and calcifying cystic odontogenictumor (CCOT) are benign odontogenic tumors having some