if1if2 df1df2df3df4 ms laser microscope (observation) objectives (observation, focus) a) b)b) figure...
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IF1IF2 DF1DF2DF3DF4
MS
LASER
microscope (observation)
objectives (observation, focus)
a)
b)
Figure 1
CCD camera(500/200 * 5= X12.5)
f=500mm condenser lens
Coaxial epi-illumination
355nm LASER
X5 infinity-corrected objectives(NA=0.13, WD=35mm)Spot diameter R=1.6mm （ theoretical ）Focal depth 263≒ mm(R=20mm)
Dichroic mirror
Half mirror
a)
b)
Figure 2c)
Figure 3
Peak picking
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.
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MS slice images
Average spectrum viewer
MS slice viewer
TIC image viewer
a)
b)
Figure 4
A B
C D
Figure 5
A
B C
D E
Figure 6
273.03904
382.82639
667.34340 795.53110 931.657030.5
1.0
1.5
2.0
2.5
5x10
347.00358441.29799
667.34340
795.53110
931.65703
1.0
1.5
2.0
2.5
4x10
200 300 400 500 600 700 800 900 m/z
321.04874
321.07601
321.09105 321.12783
321.20400
0.80
0.85
0.90
0.95
1.00
4x10
321.09463321.18384
321.21053
2
4
6
4x10
321.04895
321.09390 321.13211
0.0
0.2
0.4
0.6
0.8
1.05x10
321.04 321.06 321.08 321.10 321.12 321.14 321.16 321.18 321.20 321.22 m/z
C
B
A
D
Figure 7
E
Figure 8
Figure 9
a)
b)
Figure 10
a)
b)
c)
A
A
A
B
B
B
C
C
C
LDI MALDI/DHBA
MALDI/CHCA
246E(m/z)=495.401
771E(m/z)=490.752
86E(m/z)=336.988
132E(m/z)=536.278
2652E(m/z)=373.679
2242E(m/z)=244.300
868E(m/z)=475.115
Figure 11