http cheng.dlearn.kmu.edu.tw ppt tissueengineering
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Tissue Engineering
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http://www.youtube.com/watch?v=PEc7QXAjsL4&feature=related
Mouse Ears
1997 MIT
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http://www.youtube.com/watch?v=WvSxZ4JKQAY&feature=related
Engineering Tissue
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Corneal Tissue Engineering
Blood vessel tissue engineering
Liver tissue engineering
Cartilage tissue engineering
Bone tissue engineering
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Cell Culture( stemcell, osteoblast,
chondrocyte,
hepatocyte
Signaling
Molecules(GrowthFactors, proteins
Scaffold( ceramics
collagen, polymers)
Tissue/Organ
regeneration
Key Factors involving tissue engineering
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Cell culture
Interactive traids of responsive cells
Scaffolds
Supportive matrix
Growth factorsBioactive molecules promoting differentiation
and regeneration
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Application of tissue engineering
3D culture
Transplantation
Cellular and tissuebases product
Alternative to animal
test
http://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gif -
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Developing procedures used in cartilage tissue engineering
1st generation 2nd generation 3rd generation
Principle
approachAutologous cell
transplant
Performed tissue
flaps 3D construct
In vivo regeneration
and guided tissue
repair
Importantcomponent
Periostealflaps( )Delivery
substances and
scaffold( )
Growth factors and
biomaterials( )
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Cartilage Tissue Engineering
I. Autologous chondrocyte
1. Isolation of tissue sample from patients
2. Tissue regeneration by precursor cells
or embryonic stem cells
articular cartilage repair, nasal septal
cartilage scaffolds for cartilage for
tissue repair
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ECM ( Extra cellular Matrix)
ECM interaction directly influence cell signaling via
cell adhesion molecules such as integrinand
cadhedrin, fibronectin, collagen, laminin.
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Extracellular Matrix in relation to epithelium endothelium and
connective tissue
Epithelial cell
Basement
membrane
Endothelial lining the
capillary
Connective tissue with
interatitial matrix
Fibroblast
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http://inano.au.dk/organization/research-groups/nanobiointerfaces/research/steering-stem-cell-differentiation/
Steering Stem Cell Differentiation
Differentiation of stem cells induced by binding to extracellular
matrix components(e.g. proteins such as fibronectin, collagen
and laminin)components
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II. Scaffold for tissue construction
1. Act as shapeand guidancetemplate for
in vi t roand in vivotissue development
2. Permit 3D immobilization and maintain
differentiation
materials: hyaluronic acid, collagen, alginate,
chutosan, synthetic biodegradable poly( -
hydroxy ester) polyacetic acid,( PLIA), polyglycolic
acid( PGA), PLGA
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Chondrocyte or
Mesenchyma stem cell
Polymer fiberEmbedding medium
( hydrogel)
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Biosynthetic scaffolds
Collagen scafold
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Tissue scafold +Cellsand
Growth factors
In vivotransplant
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http://www.youtube.com/watch?v=0QJh0yc2mAI&feature=related
Engineering Organs from Scratch
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Tissue in cardiovascular structure
Advantages:1. Avoid repeat operation
2. No rejection by the patients immune system
3. Cells behave like endothelial cells may have
low thrombus formation and low infection
i.e. valves, blood vessels, vascular patches
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Basic steps in tissue engineering of a cardiac valve
Bone marrowcells harvest
Valve
scaffold
Cell- scaffold
construct
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A h t t l ft
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Approaches to create a vascular graft
Cross
linkingExogenous
chemicals
Mechanical
stimulations
Cells + tubular scaffold Endothelial lining In vivo
implantation
Smooth muscle cells
progenitor cells
collagen gel/elastin
fibrin
Extra cellular matrix
Biodegradable
polymers
Autulogous EC
Circulating EPCAllogenic EC
Embryonic stem
cell
Non-thromgenicityImmune response
Remodeling
American Journal of Transplantation 2004; 4 (Suppl. 6): 3642
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http://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755b
e79cd210VgnVCM1000000100007fRCRD#tab-Videos/09347_00
How to Build a Beating Heart
http://channel.nationalgeographic.com/series/explorer/4828/Pho
tos/3671755be79cd210VgnVCM1000000100007fRCRD#tab-
Videos/10008_00
http://channel.nationalgeographic.com/series/explorer/4828/Pho
tos/3671755be79cd210VgnVCM1000000100007fRCRD#tab-
Videos/09346_00
http://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRD -
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Artificial skin
skin substitute
Adhere to the substrate,
Durable and sufficiently elastic
Tolerate some deformation
Allow evaporative water lossProvide a microbial barrier
Promote homeostasis
Easy to use
Readily availableimmediately after injury, and elicit a
Regeneration-like response from the wound bed
without evoking an inflammatory, foreign-body, or non-
self immunologic reaction.
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Annu. Rev. Med. 2000. 51:231244
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Artificial skin
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Artificial skin
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Therapy using Artificial skin
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MSC (mesenchyma stem cells)
Proliferate in cultures
Differentiate into multiple mesenchyma lineageie. correction of genetic disorder of , i.e bone cartilage
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Cell seeding on cotton gauzeframed collagen substratum
Formation of multilayer
mesenchyma cells
Physical detachment
3D culture
Preparation of 3-D mesenchyma cells
Preparation of 3-D mesenchyma cells
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Seeding of
mesenchyma cells
confluent
mesenchyma cells
Seeding of
epithelial cells
Coculture of epithelial
and mesenchyma cells
Detachment of epithelialcellsattached
mesenchyma cell sheet
Formation of
hetero-spheroid
Preparation of 3-D mesenchyma cells
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Osteoblast
Arise from mesenchyma cells( MSC)
MSC may be differentiate into osteoblast in vitro
Cultured osteoblast may form extracellular
matrix in culture
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Bone marrow derived
osteoblast
Cultured bone
T. Uemura et al. / Biomaterials 24 (2003) 22772286
Transplantation of cultured bone cells
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Bone marrow derivedosteoblast
Cultured bone
Transplantation of cultured bone cells
T. Uemura et al. / Biomaterials 24 (2003) 22772286
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T. Uemura et al. / Biomaterials 24 (2003) 22772286
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T. Uemura et al. / Biomaterials 24 (2003) 22772286
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http://www.jove.com/details.php?id=2691
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
http://www.youtube.com/watch?v=ofiLcTs7_Ys&feature=related
Tissue Engineering -- Building Body Parts
http://www.jove.com/details.php?id=2691http://www.youtube.com/watch?v=ofiLcTs7_Ys&feature=relatedhttp://www.youtube.com/watch?v=ofiLcTs7_Ys&feature=relatedhttp://www.jove.com/details.php?id=2691