How to use the TMRCA Case Study Workbook

Kathleen Kerwin, MSPA candidate in the Predictive Analytics Department of the School

Professional Studies at Northwestern University 2015

The review version of the TMRCA Case Study workbook has the following worksheets:

‘C. Outline’ ‘Directions’ ‘C. yDNA tree’ ‘Surname definitions and origins’ ‘C. TMRCA base Haplogroup’ Then the list of individual ‘C. TMRCA SNP’

worksheets

Overview

‘C. Outline’ shows the summary of all TMRCA worksheet results

‘Directions’ describes the features and functions on the TMRCA worksheets

  ‘C. yDNA tree’ – list of member yDNA 67 marker results organized by

SNP then placed in a phylogenetic tree order.

‘Surname definitions and origins’ – list provided by Dr. Paul MacCotter

‘C. TMRCA base Haplogroup’ – the entire list of those testing positive for CTS4466. See the training video for ‘TMRCA definitions and analysis’ at: http://southirish.com/training.htm

Then the list of individual ‘C. TMRCA [by SNP]’ worksheets

Worksheet details

‘C. Outline’

This is a list of each TMRCA worksheet which uses the dataset found on the ‘C. yDNA tree’ worksheet. Note the base Haplogroup, A541 with its sub SNPs etc The current TMRCA of each worksheet is found summarized here for comparison purposes.

This worksheet provides directions and a description of the features and functions found on each TMRCA worksheet. You can also turn the messaging on/off. If you turn messaging off, you will only get limited messages when you use the function buttons on the worksheets. If on, the messaging will describe the functions selected in more detail.

‘Directions’

This is where the member yDNA dataset resides that is acquired by each TMRCA worksheet through redirection.

Note:Member yDNA entry with ‘phylo order’, ‘A151’ followed by yDNA itself

Functions available on this worksheet – see next slide

‘C. yDNA tree’

Development functions: - not found in this review versionDelete yDNA entries – clears worksheet data, this will be removed soon

Build Phylogenetic tree order – takes the data in the original dataset worksheet and places it in phylogenetic tree order.

Add Family History comments – add member family history comments to each member entry that has formatted on the ‘comments’ worksheet

Copy C. yDNA tree back to dataset – copies the data on this worksheet back to the original worksheet.

Add SNPs to dataset – add SNP data

‘C. yDNA tree’ functions

‘Surname definitions and origins’

List provided by Dr. Paul MacCotter is used in ancestral origin and surname comparisons

‘C. TMRCA base Haplogroup’

See TMRCA definition and analysis video at http://southirish.com/training.htm for details on how to use the TMRCA functionality

How to build a TMRCA wksht

1. Select a TMRCA worksheet as a template, not the base haplogroup version

2. Rename the worksheet

3. Press the ‘Clear data’ button to delete all data

4. Access the ‘C.yDNA tree’ data for the SNP to be analyzed,1. Put an = sign in cell A3, then go to the ‘C. yDNA tree’

worksheet2. Select first kit number in your set, then return to the

TMRCA wksht

5. Drag the contents of the A3 cell down until all data has been redirected in to the worksheet. Add or subtract cells as necessary.

6. Press the ‘format/reset’ button

7. Now you are ready to begin your TMRCA calculation for the included dataset

Running a TMRCA calculation

These are only the runtime directions. You’ll need to review the ‘TMRCA definition and analysis’ training video at http://southirish.com/training.htm for detailed directions.

1. Go to panel 12, then at the last data row press the ‘autocount 12’ button

2. Note the Ahaplotype row at the top of the panel. It has been set as the most common row with all duplicate rows in the panel colored light green with the mutations colored grey. Is there a better common row that can be selected?

3. Note the Calculation Box at the bottom of the panel 12. Is the confidence level > or < 80%?

4. Work through panels 12, 25, 37 and 67 markers using the ‘autocount x’ and ‘count only’ buttons.

5. At the 67 marker panel, look to see the TMRCA summary box under the calculation box. Is this number in a reasonable range?