how to bioconjugate-- read solulink's bioconjugation primer

7/28/2019 How to Bioconjugate-- Read Solulink's Bioconjugation Primer

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^ejujiga @ienegbumktieg \riodrBkgukry 0

! ; 848'304'


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Cer oerd igceroktieg visit www'sejujiga'neo

k) Jigadrs oust `d ignerperktdf eg ìeoejdnujds ig k oijf*

negtrejjk`jd okggdr

`) Vhd ighdrdgt ìejeminkj ugntieg e thd ìeoejdnujds oust

`d ugkdntdf ktdr oefiinktieg kgf negbumktieg

n) Vhd negbumktieg rdkntieg ennurs firdntjy upeg oixigm thd

twe oefiidf ìeoejdnujds* prddrk`jy get rdquirigm

kffitieg e kg exifkgt* rdfuntkgt* er odtkj

f) Oefiidf ìeoejdnujds krd stk`jd evdr dxtdgfdf pdriefs

d) Ceroktieg e thd nevkjdgt jigakmd is stk`jd ugfdr k rekf

pH rkgmd kgf kt djdvktdf tdopdrkturds

) [ukgtiinktieg e èth ignerperktdf jigadrs kgf

igkj negbumktd is rdkfijy pdrerodf* i'd* thdy krd

spdntrepheteodtrinkjjy trkndk`jd

m) Negbumktieg ennurs ig ùdrdf kqudeus sejutiegs* kt k

physiejeminkj pH

h) ^teinhieodtrinkjjy dinidgt (d'm'* ;2;)

i) Ckst rdkntieg aigdtins

b) Ge ugfdsirk`jd nevkjdgt sifd rdkntiegs furigm oefiinktieg

a) Ge djdntrestktin%hyfrepheìn igtdrkntiegs

j) Jigadrs nkg `d ignerperktdf eg k vkridty e ìeoejdnujds*

ignjufigm ejimes kgf pdptifds* threumh sejif phksd sygthdsis

Vhd njkssinkj odthef usdf te prdpkrd nevkjdgt negbumktds okads

usd e thd okjdioife%thiej neupjigm pkir'

Fiskfvkgtkmds e thd okjdioife%thiej pkir ignjufd2

k) Vhd kqudeus igstkìjity (hyfrejysis) e thd jigadr ugntiegkj

mreup (i'd'* okjdioifd) eg ìeoejdnujds furigm negbumktieg

`) Vhd gddf te pretdnt thd thiej mreup

n) Vhd sjew aigdtins e thd rdkntieg

f) Vhd gddf te kntivktd thd pretdntdf thiej mreup with k stregm

rdfunigm kmdgt (d'm'* FVV er VND\)

d) Vhd gddf te kntivktd thd pretdntdf thiej mreup `y kffitieg

e k stregm rdfunigm kmdgt (d'm'* FVV)

) Vhd petdgtikj er ugfdsirk`jd heoe/fiodrs vik fisujifd

`rifmd eroktieg

m) Njdkvkmd kgf%er pkrtikj rkmodgtktieg e pretdigs `y

rdfuntieg e fisujifd ègfs

h) Pdfundf er igkntivd pretdig ugntieg

Gdxt

Vhd sejutieg''' v v


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! ; 848'304'


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nhreoepherd k`ser`s kt >46 go with k oejkr dxtigntieg

nedinidgt e 09*' Vhd HyGin%6C@ negbumktieg neupjd' Jimktieg e HyGin kgf 6C@

with kgijigd/nktkjyzdf hyfrkzegd jimktieg `dtwddg 3/hyfrkzigepyrifyj

(HyGin)/ugntiegkjizdf pdptifd 6 kgf dgzkjfdhyfd 4 (jdt) ejjewdf y

TS/SI^ spdntresnepy (>6< go)'


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! ; 848'304'


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\retdig/Ejimegunjdetifd Negbumktieg

Vhd kìjity te dinidgtjy kgf rdprefuni`jy prdpkrd kgtièfy/

ejimegunjdetifd negbumktds hks jioitdf thdir dxpjeitktieg ig

oujtipjdxdf fikmgestin kssskys'

êjujigas HyGin%6C@ ìenegbumktieg neupjd* ks kppjidf te

thd negbumktieg e ejimegunjdetifds with kgtièfids* is

steinhieodtrinkjjy dinidgt kgf himh yidjfigm* negvdrtigm 794$

kgtièfy te kgtièfy/ejimegunjdetifd negbumktd (Cimurd =)'

Curthdroerd* negbumktiegs e ejimeodrs e 0/tdroigus e kg ejimegunjdetifd `y k siopjd*

vdry himh/yidjfigm negvdrsieg e k >/ er 4/koige ejime usigm

suje/^/6C@' Kjtdrgktivdjy* k 6C@/phespherkoifitd er firdnt

ignerperktieg eg thd 4/tdroigus nkg `d usdf' îopjd oixigm

kgf ignu`ktieg e thd 6C@/ ejimegunjdetifd with k 0> ejf dxndss

e thd HyGin/pdptifd* ejjewdf `y puriinktieg `y fikijtrktieg*

prefunds thd negbumktd ig gdkr qukgtitktivd yidjf'

Cimurd 3' ^trunturds e @en/HGK (;) kgf Coen/Jys/d/(@en/HGK)' Vhdsd rdkmdgts

krd usdf te ignerperktd HyGin mreups eg thd G/ kgf N/tdroigi e pdptifds*

rdspdntivdjy' kgf 6C@/phespherkoifitd is usdf te ignerperktd 6C@ mreups eg

thd 4/tdroigus e ejimegunjdetifds'

Cimurd 4' \dptifd/ejimegunjdetifd negbumktieg' ^F^/\KMD mdj e negbumktds

visukjizdf with TS kna/shkfew' Negbumktieg e twe G/tdroigkj HyGin/

oeficdf pdptifds ;0; kgf ;00 (Jkgds 0 kgf >) rdkntdf with k 4/6C@ oeficdf

;9/odr ejime (Jkgd ;) te yidjf thd pdptifd/ejime negbumktds (Jkgds 6 kgf 4)'

Ks sddg ig jkgds 6 kgf 4* negbumktd eroktieg wks dcnidgt kt k ;2> rdkntieg

steinhieodtry (ejime2pdptifd)' Negbumktd ègf stkìjity wks tdstdf kt 94EN ig

\@^ er twe hr (Jkgds 3 kgf =)'


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! ; 848'304' ejimegunjdetifds pdr

kgtièfy'

Ig k sdnegf `rdkathreumh er kgtièfy/ejimegunjdetifd

negbumktieg* wd fdvdjepdf k odthef te puriy thd negbumktd

`y kfserptieg te k prepridtkry okmgdtin kigity oktrix thkt

kjjews rdoevkj e dxndss 6C@/ejimegunjdeitfd* ejjewdf `y

djutieg e thd puriidf negbumktd usigm oijf djutieg ùdrs'

Vhd evdrkjj yidjf e thd kgtièfy/ejimegunjdetifd negbumktdis >46 go) ìs/kryjhyfrkzegd nhreoepherd igte

thd jigadr kro ig èth ìetig kgf fimeximdgig oefiinktieg

rdkmdgts* thd NhreokJiga @ietig kgf NhreokJigaFimeximdgig (Cimurd 8)' ^pdntrepheteodtrin qukgtiinktieg

e thd ignerperktieg e thdsd nhreoepherin hkptdgs is

strkimhterwkrf kgf himhjy rdprefuni`jd' îopjy odksurigm thd

K08< kgf K>46 k`ser`kgnd e thd oefiidf pretdigs yidjfs èth

thd pretdig negndgtrktieg kgf guo`dr e ignerperktdf jk`djs

(Cimurd =)'

Cimurd =' Kgtièfy/ejimegunjdetifd negbumktieg' Vhd sijvdr stkigdf ^F^/\KMD

mdj prdsdgts fktk er thd negbumktieg kgf puricnktieg e k 6) kgf k 0


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@ieoejdnujd Iooeìjizktieg

Iooeìjizktieg e pretdigs* dspdnikjjy kgtièfids* is nritinkjjy

iopertkgt ig okgy ìejeminkj kppjinktiegs1 ignjufigm

ioougeprdnipitktieg* ndjj dgrinhodgt* kgtimdg puriinktieg* kgf

ioougefdtdntieg' Cer this rdkseg* rdsdkrnhdrs etdg rdquird thkt

pretdigs `d iooeìjizdf eg dithdr k okmgdtin er geg/okmgdtin

surknd'

Vhdrd krd twe okig odthefs usdf te iooeìjizd pretdigs2 ;)

iooeìjizktieg e ìetigyjktdf pretdigs eg strdptkvifig/oefiidf

surknds er 0) firdnt nevkjdgt iooeìjizktieg eg kntivktdf

surknds' êjujiga hks dgmigddrdf its negbumktieg nhdoistry te

edr èth kjtdrgktivds withig thd GkgeJiga kgf OkmgkJiga

koijy e prefunts'

Cer kgtièfy jk`djigm* êjujiga fdsimgdf kgf prefundf thd

NhreokJiga @ietig kgf NhreokJiga Fimeximdgig Egd/^het

Aits * whinh ignjufd dvdrythigm rdquirdf te jk`dj* puriy* kgf

qukgtiy ignerperktieg e thdsd jk`djs reo k sigmjd ;


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! ; 848'304' te ;4 tiods mrdktdr `ietig `igfigm nkpknity thkg kgy

ethdr neoodrnikjjy kvkijk`jd okmgdtin `dkf (Cimurds ;


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;' Ok R* Ji [* Kg H dt kj' (083* I' 02084/08='

4' Awegm MK* Pkfu NM* Hwkgm A dt kj' (0


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;4' ]egm NH* Orua FF* Jdd ] dt kj' (0


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! ; 848'304';' ^tdig`drm/Vktokg* M* Huygh O* @kradr F dt kj' (00' Jiu M* Feu ^* Hd B dt kj' (0>' Jiu M* Feu ^* Okrfiressikg M dt kj' (0> I' 423==/38>'

>3' Knhijjds A kgf Aidfrewsai S (0=' Ajigd ON* Fudwdr FJ* Pdfokg B] dt kj' (09' Rhegm X* Jdgm J* @ditig K dt kj' (0* I'

032>64>/>638'

60' Knhijjds A kgf Aidfrewsai MS (0'

6>' Aezjev IK* Odjgya \N* ^treos`erm AD dt kj' (0


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64' Rhegm X* Pdygejfs P* Aiff BP dt kj' (0

how to bioconjugate-- read solulink's bioconjugation primer

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