home | the university of sheffield - …/file/sample_c… · web viewspillages 1) small spillages...
TRANSCRIPT
S. Foster COSHH assessments Feb. 2014
COSHH ASSESSMENTS
S. FOSTER LABORATORY
Every experimental procedure that involves chemicals and biological agents that present a potential hazard must be assessed for risk to human health. These assessments fall under the Control of Substances Hazardous to Health (COSHH) regulations.
Prior to conducting an experiment for the first time:
1. Consult Table 1 “Titles of COSHH assessments”
2. Look up the detailed assessment using the COSHH protocol number
3. Make sure you read and understand the assessment, paying particular attention to:
o RISK ASSESSMENT & CONTROL MEASURESo ACCIDENT/SPILLAGE PROCEDUREo WASTE DISPOSAL
4. If the procedure you intend to follow is not covered by these assessments, then you should conduct a risk assessment before you start. Do this using a blank form (see end of folder), and ensure that the completed form is typed up and added to the folder as soon as possible.
Notes:
Some techniques are covered by more than one protocol e.g. #s 19 and 18 cover SDS PAGE and sample preparation respectively
Risk Phrases can be found on the web sites of most manufactures’ web sites- their definitions are listed on page 2 - 4
If in doubt about how to write a COSHH assessment, contact S. Foster.
In the protocols, “Local Rules” refers to the risk assessments for handling ACDP hazard Group 2 pathogens and GMM class II organisms – WT2013_06 and GM2013_05.
S. Foster COSHH assessments Feb. 2014
ALL workers in the lab are informed about Coshh forms before starting in the lab.
DATE NAME UGS/PGS/PhD student or other
SIGNATURE
S. Foster COSHH assessments Feb. 2014
RISK PHRASES – PAGE 1R1 Explosive when dryR2 Risk of explosion by shock, friction, fire or other sources of ignitionR3 Extreme risk of explosion by shock, friction, fire or other sources of
ignitionR4 Forms very sensitive explosive metallic compoundsR5 Heating may cause an explosionR6 Explosive with or without contact with airR7 May cause fireR8 Contact with combustible material may cause fireR9 Explosive when mixed with combustible materialR10 FlammableR11 Highly flammableR12 Extremely flammableR14 Reacts violently with waterR14/15 Reacts violently with water, liberating extremely flammable gasesR15 Contact with water liberates extremely flammable gasesR15/29 Contact with water liberates toxic, extremely flammable gasesR16 Explosive when mixed with oxidising substancesR17 Spontaneously flammable in airR18 In use, may form flammable/explosive vapour-air mixtureR19 May form explosive peroxidesR20 Harmful by inhalationR20/21 Harmful by inhalation and in contact with skinR20/21/22 Harmful by inhalation, in contact with skin and if swallowedR20/22 Harmful by inhalation and if swallowedR21 Harmful in contact with skinR21/22 Harmful in contact with skin and if swallowedR22 Harmful when swallowedR23 Toxic by inhalationR23/24 Toxic by inhalation and in contact with skinR23/24/25 Toxic by inhalation, in contact with skin and if swallowedR23/25 Toxic by inhalation and if swallowedR24 Toxic in contact with skinR24/25 Toxic in contact with skin and if swallowedR25 Toxic if swallowedR26 Very toxic by inhalationR26/27 Very toxic and in contact with skinR26/27/28 Very toxic by inhalation, in contact with skin and if swallowedR26/28 Very toxic by inhalation and if swallowedR27 Very toxic in contact with skinR27/28 Very toxic in contact with skin and if swallowedR28 Very toxic if swallowedR29 Contact with water liberates toxic gasR30 Can become highly flammable in useR31 Contact with acids liberates toxic gasR32 Contact with acids liberates very toxic gasR33 Danger of cumulative effectsR34 Causes burnsR35 Causes severe burnsR36 Irritating to eyesR36/37 Irritating to eyes and respiratory systemR36/37/38 Irritating to eyes, respiratory system and skinR36/38 Irritating to eyes and skin
S. Foster COSHH assessments Feb. 2014
R37 Irritating to respiratory systemR37/38 Irritating to respiratory system and skinR38 Irritating to skinR39 Danger of very serious irreversible effectsR39/23 Toxic: danger of very serious irreversible effects through inhalationR39/23/24 Toxic: danger of very serious irreversible effects through inhalation and in
contact with skinR39/23/24/25 Toxic: danger of very serious irreversible effects through inhalation, in
contact with skin and if swallowedR39/23/25 Toxic: danger of very serious irreversible effects through inhalation and if
swallowedR39/24 Toxic: danger of very serious irreversible effects in contact with skinR39/24/25 Toxic: danger of very serious irreversible effects in contact with skin and if
swallowedR39/25 Toxic: danger of very serious irreversible effects if swallowedR39/26 Very Toxic: danger of very serious irreversible effects through inhalationR39/26/27 Very Toxic: danger of very serious irreversible effects through inhalation
and in contact with skinR39/26/27/28 Very Toxic: danger of very serious irreversible effects through
inhalation, in contact with skin and if swallowedR39/26/28 Very Toxic: danger of very serious irreversible effects through inhalation and
if swallowedR39/27 Very Toxic: danger of very serious irreversible effects in contact with skinR39/27/28 Very Toxic: danger of very serious irreversible effects in contact with skin and
if swallowedR39/28 Very Toxic: danger of very serious irreversible effects if swallowedR40 Limited evidence of a carcinogenic effectR40/20 Harmful: possible risk of irreversible effects through inhalationR40/20/21 Harmful: possible risk of irreversible effects through inhalation and in contact
with skinR40/20/21/22 Harmful: possible risk of irreversible effects through inhalation, in
contact with skin and if swallowedR40/20/22 Harmful: possible risk of irreversible effects through inhalation and if
swallowedR40/21 Harmful: possible risk of irreversible effects in contact with skinR40/21/22 Harmful: possible risk of irreversible effects in contact with skin and if
swallowedR40/22 Harmful: possible risk of irreversible effects if swallowedR41 Risk of serious damage to eyesR42 May cause sensitisation by inhalationR43 May cause sensitisation by skin contactR44 Risk of explosion if heated under confinementR45 May cause cancerR46 May cause heritable genetic damage
S. Foster COSHH assessments Feb. 2014
R48 Danger of serious damage to health by prolonged exposureR48/20 Harmful: danger of serious damage to health by prolonged exposure
through inhalationR48/20/21 Harmful: danger of serious damage to health by prolonged exposure
through inhalation and in contact with skinR48/20/21/22 Harmful: danger of serious damage to health by prolonged exposure
through inhalation, in contact with skin and if swallowedR48/20/22 Harmful: danger of serious damage to health by prolonged exposure
through inhalation and if swallowedR48/21 Harmful: danger of serious damage to health by prolonged exposure in
contact with skinR48/21/22 Harmful: danger of serious damage to health by prolonged exposure in
contact with skin and if swallowedR48/22 Harmful: danger of serious damage to health by prolonged exposure if
swallowedR48/23 Toxic: danger of serious damage to health by prolonged exposure
through inhalationR48/23/24 Toxic: danger of serious damage to health by prolonged exposure
through inhalation and in contact with skinR48/23/24/25 Toxic: danger of serious damage to health by prolonged exposure
through inhalation, in contact with skin and if swallowedR48/23/25 Toxic: danger of serious damage to health by prolonged exposure through
inhalation and if swallowedR48/24§ Toxic: danger of serious damage to health by prolonged exposure in contact
with skinR48/24/25 Toxic: danger of serious damage to health by prolonged exposure effects
in contact with skin and if swallowedR48/25 Toxic: danger of serious damage to health by prolonged exposure effects if
swallowedR49 May cause cancer by inhalationR50 Very toxic to aquatic organismsR50/53 Very toxic to aquatic organisms, may cause long-term adverse effects in
the aquatic environmentR51 Toxic to aquatic organismsR51/52 Toxic to aquatic organisms, may cause long-term adverse effects in the
aquatic environmentR52 Harmful to aquatic organismsR52/53 Harmful to aquatic organisms, may cause long-term adverse effects in
the aquatic environmentR53 May cause long-term adverse effects in the aquatic environmentR54 Toxic to floraR55 Toxic to faunaR56 Toxic to soil organismsR57 Toxic to beesR58 May cause long-term adverse effects in the environmentR59 Dangerous for the ozone layerR60 May impair fertilityR61 May cause harm to the unborn childR62 Possible risk of impaired fertilityR63 Possible risk of harm to the unborn childR64 May cause harm to breast-fed babiesR65 Harmful: may cause lung damage if swallowedR66 Repeated exposure may cause skin dryness or crackingR67 Vapours may cause drowsiness and dizziness
S. Foster COSHH assessments Feb. 2014
R68 Possible risk of irreversible effects
S. Foster COSHH assessments Feb. 2014
Table 1: Titles of COSHH assessments:
Protocols/experiments COSHH protocol number
Growth and handling of microorganisms, (HG1 and 2, including GMMs)
14SF044
Electroporation and transformation of bacteria 14SF045Preparation of protein samples 14SF046Staining bacterial cultures 14SF047Staining and immunostaining of cells 14SF048Approved disinfection methods 14SF049Preparation and handling of serum 14SF050Storage of cultures at -80oC or in liquid nitrogen 14SF051Agarose gel electrophoresis 14SF052Preparation of genomic and
plasmid DNA from bacteria 14SF053Extraction of DNA from Agarose gels 14SF054Recombinant DNA techniques- digestion, ligation, modification etc.
14SF055
SDS – PAGE 14SF056Preparation of samples for
PAGE (1D and 2D) 14SF057Western blotting – dry and wet 14SF058Antibody based detection of proteins on gels 14SF059ELISA 14SF060BCA protein assay 14SF061Coomassie blue staining 14SF062Ponceau staining 14SF063Extraction of proteins from gels and trypsin digestion 14SF064Glycosylation assay 14SF065Isoelectric focussing gel 14SF066Preparation and rp-HPLC analysis of peptidoglycan 14SF067
Use of glutaraldehyde for fixation of cells tissues 14SF068Preparation of buffer solutions 14SF069Purification of proteins: glutathione & nickel agaroseAffinity chromatography
14SF070
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF044
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FORREASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Growth and handling of microorganisms, including Hazard Group 1 and Hazard goup 2 organisms,including GMMs
PROTOCOL/SUBSTANCE RISK PHRASES
RISK STATEMENTS
Cultures of Enterococcus faecalis, Enterococcus faecium, Streptococcus pyogenes, Streptococcus suis, Streptococcus peumoniae, Bacillus cereus, Listeria monocytogenes (HG2), Staphylococcus aureusCultures of Escherichia coli and Lactococcus lactis (HG1)
AntibioticsKanamycin
Tetracyclin
Ampicillin
Neomycin
Chloramphenicol
Fusidic acid
Rifampicin
Gentamycin
Erythromycin
N/A
R61
R36/37/38
R36/37/38R42/43
R42/43
R45
R22
R22/36/37/38
R42/43
R42/43
N/A
May cause harm to the unborn childIrritating to eyes, respiratory system and skin
May cause sensitisation by inhalation or skin contact
May cause cancer
Harmful when swallowed
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)See risk assessment GMO2015_05 for details concerning the organisms manipulated, control and measures.All antibiotics are weighed under the hood with appropriate PPE (gloves, labcoat); antibiotic solutions require gloves at all times.
S. Foster COSHH assessments Feb. 2014
PROTOCOL/SUBSTANCE RISK PHRASES
RISK STATEMENTS
ACCIDENT/SPILLAGE PROCEDURE
Spillages1) Small spillages (< 25 ml) – soak up majority of the solution using absorbent “Blue Roll” towels and place these in an autoclave bag for disposal. Flood the area with 1% Virkon, leave for at least 10 minutes.Wipe area dry with absorbent towels, and place these in autoclave waste.
2) Larger (> 25ml spillages). a) Decontaminate area as in (a) above. b) Anything which cannot be decontaminated (paper, pencils etc.) must be discarded into the autoclave waste. c) Inform S. FOSTER d) REPORT THIS SPILL AS A DANGEROUS OCCURRENCE
WASTE DISPOSALSee risk assessment GMO2015_05 for details
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF045
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Electroporation and transformation of bacteria
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
E. coli culturesCalcium chloride; Use of electroporation apparatus (high voltage)
N/AN/AN/A
RISK ASSESSMENT & CONTROL MN/AEASURES (State if health surveillance required)
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Flood area with 1% Virkon. Mop up with paper towels and dispose in autoclave waste
WASTE DISPOSAL
Decontaminate HG1 bacterial cultures/supernatants with virkon 1% ; discard contaminated plasticware in biological waste.
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF046
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Preparation of protein samples
PROTOCOL/SUBSTANCE RISK PHRASE RISK STATEMENT
SDS
Glycine Urea TCA
Acetone
R20/22, R36/37/38, R42
N/AN/AR35-50/53
R11 R-36 R66/67
Harmful in contact with skin and if swallowed; irritating to eyes, respiratory system and skin; may cause sensitisation by inhalation
Causes severe burns. Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environmentEye Irritant. Repeated exposure may cause skin dryness/cracking. Vapour may cause dizziness.
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
SDS is an irritant in powder form. It is harmful when in contact with skin or when inhaled or swallowed. Wear gloves at all times. Wear face mask when weighing out powder
When using sonicator, make sure sound proof door is closed or that ear protection is wornGood Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Culture spills: Flood area with 1% Virkon. Mop up with paper towels and dispose in autoclave wasteSDS spillage: wash area with water and detergent. If SDS is ingested or comes in contact with eyes, seek medical attention.
WASTE DISPOSALTCA must be disposed of as Special waste.Acetone in non-chlorinated waste solvent bottle
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF047
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Staining bacterial cultures
PROTOCOL/SUBSTANCE RISK PHRASE RISK STATEMENT
Gram's crystal violet
Gram's iodineGram's safranin Gram's Decolorizer (alcohol or acetone)
R22, R41, R45, R46, R50/53
N/AR41R10
Harmful when swallowed; risk of serious damage to eyes; may cause cancer and heritable genetic damage; very toxic to aquatic organisms, may cause long term adverse effects to the aquatic environment
Risk of serious damage to eyesFlammable
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)All stains are potentially hazardous. Many are carcinogens, harmful if swallowed Alcohol and acetone are highly flammableGood Laboratory Practice
ACCIDENT/SPILLAGE PROCEDUREWash area with water and detergentIf affected, seek medical attention
WASTE DISPOSAL Waste stains and alcohol/acetone are washed down sink.Slides are placed in sharps boxBacteria are placed in autoclave waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF048
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Staining and immunostaining of cells
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
4% paraformaldehyde
bisBenzimide H 33258 (Hoescht stain)
R20/22, R34, R40, R43
22-36/38
Harmful by inhalation and if swallowed; causes burns; skin contact may cause sensitisation; limited evidence of carcinogenic effect. Harmful if swallowed, Irritating to the eyes, Irritating to the skin
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)Paraformaldehyde: avoid contact or inhalation. Keep in a fume cupboard whenever possible
Hoescht stain: avoid contact, keep in fume cupboard when in powder form, make pup into solution and use that.
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Spillage: wash affected area with water and detergentHoescht: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
WASTE DISPOSALWash down sink with copious amounts of water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF049
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Approved disinfection methods
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
EthanolChlorosHycolin
Virkon
R11 R21/22R24/25, R34
R38, R41
Highly flammableHarmful in contact with skin or if swallowedToxic in contact with skin or if swallowed; causes burnsIrritating to skin, risk of serious damage to eyes.
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory PracticeWear gloves at all times
If affected by exposure to Chloros or Hycolin, remove from exposure site and seek medical advice
ACCIDENT/SPILLAGE PROCEDURE
Spillage: rinse affected site with water.
WASTE DISPOSALRinse waste down sinkPlace tissues etc in clinical waste.
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF050
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Preparation and handling of serum
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Rodent or human blood and sera
N/A Human blood may carry infectious diseases.
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Risk of contamination of human blood or sera with pathogens, e.g. HIV, hepatitis virus
Refer to the Protocol for “Handling ACDP Hazard Group Two Pathogens” for detailed protocol
Inactivate human sera by heating at 56oC for 60 minutes
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Spillage: treat area with Virkon and discard towels as clinical wasteIn the event of any material entering the body in any way, e.g. through a cut, seek medical advice immediately.
WASTE DISPOSALDiscard in autoclave waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF051
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Storage of cultures at -80oC or in liquid nitrogen
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Liquid NitrogenSolid CO2 (dry ice)
Hazardous due to burns and asphyxiation
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Liquid nitrogen will freeze any tissue upon contact. Risk of eye injury if liquid enters eye.
Use only with thick gloves and a face shield.
Solid CO2 (dry ice) will cause burns on contact with skin. Always wear thick gloves. Risk of asphyxiation if large quantities of dry ice are kept in an enclosed area, e.g. a lift.
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Liquid nitrogen and dry ice spillages: leave area and wait until evaporation occursBurns and other injuries –seek medical advice
WASTE DISPOSALThere is no designated waste disposal. Leave either chemical in a safe place to evaporate or sublime
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF052
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Agarose gel electrophoresis
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Ethidium bromide
UV light
R22, R26, R36/37/38, R68
Harmful when swallowed; very toxic by inhalation; irritating to eyes, respiratory system and skin, possible risk of irreversible effectsHazardous
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)Ethidium bromide is very toxic by inhalation, ingestion and skin contact. Causes skin/eye irritation. Irritating to mucous membranes & upper respiratory tract.
Avoid all skin contact or inhalation & observe GLP
Wear gloves. Never handle powder.
UV light is a powerful mutagen. Full-face UV protective face shield must be worn, and no skin should be exposed to UV light.
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Spillage: Wipe up spill with towels – place in clinical wasteWash area with water and detergent. Seek medical advice if ethidium bromide is internalised or if concentrated solution contacts skin.
WASTE DISPOSALDiscard gel buffers down sink with copious amounts of waterPlace used gels in clinical waste.
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF053
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Preparation of genomic and plasmid DNA from bacteria
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Plasmid prep kitsPhenol and phenol/chloroform
Lysozyme, mutanloysin
R20/22 R23/24/25, R34, R36, R45, R46
R23/24/25
Harmful by inhalation and if swallowedToxic by inhalation, contact with skin, or if swallowed; causes burns; irritating to eyes; may cause cancer or heritable genetic damage.Toxic by inhalation, contact with skin or if swallowed
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory PracticeWhen using phenol or phenol/chloroform, always wear eye protection and gloves.
ACCIDENT/SPILLAGE PROCEDURE
In case phenol solution is in contact with skin, wash with copious amounts of water and swab area with polyethylene glycol until odour has gone. If affected seek medical advice.
WASTE DISPOSALDiscard all solutions as autoclave waste except phenol/phenol chloroformPhenol / phenol chloroform – place in “chlorinated hydrocarbon waste” pot
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOLNUMBER: 14SF054
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Extraction of DNA from Agarose gels
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Qiaex gel extraction kitBuffer QX1 Scalpels
R22 Harmful if swallowed
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Buffer QX1 contains sodium perchlorate, a strong oxidising agent.
Avoid contact with strong acids, organics, fine powdered metals and reducing agents
Avoid contact with skin and eyes
Wear Safety glasses and gloves
Scalpels are sued to excise gel fragments. Handle with care.
Good Laboratory PracticeACCIDENT/SPILLAGE PROCEDURE
Remove from exposure, rinse affected site with water.If affected, seek medical advice
WASTE DISPOSALReagents – autoclave wasteScalpels – Cin Bins – do not keep for re-use
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF055
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Recombinant DNA techniques- digestion, ligation, modification etc.
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
DNA substrates, enzymes containing glycerol, buffers
N/A
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSALPlace in autoclave waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF056
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
SDS – PAGE
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
SDS
Acrylamide and bis-acrylamide
TEMED
R21/22, R36/37/38, R42
R23/24/25, R33, R45, R46
R11, R20/22, R34
Harmful in contact with skin and if swallowed; irritating to eyes, respiratory system and skin; may cause sensitisation by inhalationToxic by inhalation, in contact with skin and if swallowed, danger of cumulative effects, may cause cancer and heritable genetic damage.Carcinogen, mutagen, teratogenHighly flammable, harmful by inhalation and if swallowed, causes burns
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Acrylamide is highly toxic and is easily absorbed by the skin
TEMED is highly flammable, irritant
Use mask when weighing out solid SDS Good Laboratory Practice
Wear safety glasses and gloves
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSALUnpolymerised acryalamide – polymerise with TEMED and ammonium persulphate and treat as clinical wastePolymerised gels - clinical waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF057
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Preparation of samples for PAGE (1D and 2D)
PROTOCOL/SUBSTANCE RISK PHRASE RISK PHRASE
SDS
DTT
mercaptoethanol
R21/22, R36/37/38, R42
R22, R36/37/38
R22, R24, R34, R51/53
Harmful in contact with skin and if swallowed; irritating to eyes, respiratory system and skin; may cause sensitisation by inhalationHarmful if swallowed; irritant to eyes, respiratory system and skinHarmful if swallowed; toxic in contact with skin; causes burns; toxic to aquatic organisms and may cause long term adverse effects in the aquatic environment.
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice mercaptoethanol - do not breath vapour. Handle stock solution in the fume cupboardSDS - powder is an irritant. Wear mask when weighing outGood laboratory practice
ACCIDENT/SPILLAGE PROCEDURE
Wash area with water and detergent. Seek medical advice if affected.
WASTE DISPOSAL
Small quantities (microlitres) in autoclave waste; larger quantities dispose by sink with copious amounts of water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF058
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Western blotting – dry and wet
PROTOCOL/SUBSTANCE RISK PHRASE RISK STATEMENT
Methanol
Acetic acid
R11, R39/23/24/25
R10, R35
Highly flammable. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowedFlammable, causes severe burns
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
ACCIDENT/SPILLAGE PROCEDURE
Wipe spills with tissues, water and detergent
WASTE DISPOSAL
Discard reagents down sink
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF059
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Antibody based detection of proteins on gels
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
ECL reagents 1 and 2Antibody conjugatesPrimary antibodies
N/AN/AN/A
No known hazardNo known hazardIf human, potentially could contain viruses
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory PracticeIf antibodies used are human origin, refer to Local Rules before starting workAll human sera must be heat treated prior to handling
ACCIDENT/SPILLAGE PROCEDURE
Wash area with water and detergent
WASTE DISPOSAL
Place gels in clinical waste; solutions down sink with excess water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF060
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
ELISA
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Primary antibodyAntibody conjugatesOPD
citric acid30% hydrogen peroxide solution3M sulphuric acid (16%)
N/AN/AR20/21, R25, R36, R40, R43, R50/53, R68
R41R34
R35
No known hazards, but if human may contain virusesNo known hazardsHarmful by inhalation and in contact with skin, toxic if swallowed, irritating to eyes, limited evidence of a carcinogenic effect, may cause sensitisation by skin contact, very toxic to aquatic organisms, may cause long term adverse effects to the aquatic environmentRisk of serious damage to eyesCauses burns
Causes severe burns
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
ACCIDENT/SPILLAGE PROCEDURE
WASTE DISPOSALPlace in autoclave waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER:14SF070
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Purification of proteins: glutathione and nickel agarose affinity chromatography
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Nickel Agarose resin
Imidazole Glutathione sepharoseGlutathione+ other things ??
R10, R22, R40, R42/43
R22, R34N/A?N/A
Flammable; harmful when swallowed, limited evidence of carcinogenic effect, may cause sensitisation by skin contact or by inhalationHarmful when swallowed, causes burns
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice , wear gloves
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSALPlace in clinical waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF61
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
BCA protein assay
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
NaCO3; NaHCo3 Na tartrate NaOH Bicinchoninic acid CuSO4
BSA in 0.05% Na azide
R36R35R36/37/38R22, R36/38R28, R23
Irritating to eyesCauses severe burnsIrritating to eyes, respiratory system and skinHarmful when swallowed; irritating to eyes and skinVery toxic if swallowed, toxic by inhalation
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good laboratory practice
Wear glovesACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSALPlace small amounts in clinical waste; large amounts wash down sink with copious amounts of water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF062
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Coomassie blue staining
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Coomassie brilliant blueMethanol
Acetic acid
N/AR11, R39/23/24/25
R10, R35
Highly flammable. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowedFlammable, causes severe burns
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice.
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with waterIf ingested or inhaled, seek immediate medical attention
WASTE DISPOSALPour solutions down sink with copious amounts of water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF063
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Ponceau staining
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Acetic acid
Ponseau S
R10, R35
R36/37/38, R51/53
Flammable, causes severe burns
Irritating to eyes, respiratory system and skin; toxic to aquatic organisms and may cause long-term adverse effects in the aquatic environment
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water
WASTE DISPOSALPour solutions down sink with copious amounts of water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF064
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Extraction of proteins from gels and trypsin digestion
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Tris, EDTA, NaCl, MgCl
MnCl
Sodium deoxycholate
Urea
R36/37/38
R22, R36/37/38, R40
R22, R52
N/A
Irritating to eyes, respiratory system and skin
As above plus harmful when swallowed, limited evidence of carcinogenic effect
Harmful if swallowed; harmful to aquatic life
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSALPour solutions down sink with copious amounts of water; place gel fragments in clinical waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF065
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Glycosylation assay
PROTOCOL/SUBSTANCE RISK PHRASES
RISK STATEMENTS
Sodium-meta-perjodate
Sodium metabisulfite
N,N-Dimethylformamide
Digoxigenin-3-O-succinyl-amidocapron acid-hydrazid-hydro-chloride
4-Nitro blue tetrazolium chloride
Acetic acid
Ponceau S
R7, R20/22
R22, R31, R36/37, R41
R61, R20/21, R36
R26/27/28, R33, R36
R20/21/22
R10, R35
R36/37/38
May cause fire, harmful by inhalation or if swallowed
Harmful when swallowed, contact with acids liberates toxic gas, irritating to eyes and respiratory system, risk of serious damage to eyes
May cause harm to the unborn child, harmful by inhalation and in contact with skin, irritating to eyes
Very toxic by inhalation, in contact with skin or if swallowed, danger of cumulative effects; irritating to eyes
Harmful by inhalation, if swallowed or in contact with skin
Flammable,causes severe burns
Irritating to eyes, respiratory system and skin
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)Good laboratory practice
Although small amounts are used, take extreme care.
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergentSeek medical attention if affected
WASTE DISPOSALPlace in clinical waste
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF066
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Isoelectric focussing gel
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Tris
CHAPS bufferUrea
R36/37/38
R36/37/38 N/A
36/37/38: Irritating to eyes, respiratory system and skin
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good Laboratory Practice
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSALPlace gels in clinical wasteLiquid waste should be washed down sink with excess water
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF067
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Preparation and rp-HPLC analysis of peptidoglycan
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTSAmmonium phosphateTCATFA
Methanol
Acetonitrile
Na-azide
N/AR35-50/53R20/R35/R52/53
R11, R39/23/24/25
R11-R23/24/25
R28, R23
N/ACauses severe burns. Highly corrosive
Highly flammable. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowedHighly flammable (flash point +2°C, Toxic
Very toxic if swallowed, toxic by inhalation
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good laboratory practiceAll buffers containing TFA, Methanol and acetonitrile should be prepared under the hood, and transferred into sealed bottles to avoid inhalation.
ACCIDENT/SPILLAGE PROCEDURE
Good laboratory practice
WASTE DISPOSAL
TCA must be disposed of as Special waste.Methanol volumes less than 50ml are disposed of in the sinkDispose of large amounts of HPLC buffer containing diluted chemicals (wash down sink with copious amounts of water)
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF068
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Use of glutaraldehyde in scanning EM and fixation of cells tissues
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Glutaraldehyde R20/21/22R36/37R42
Harmful by inhalation, in contact with skin and if swallowedIrritating to eyes and respiratory systemMay cause sensitization by
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Glutaraldehyde is toxic and must be used with extreme care. Allergy due to sensitisation is common.It must only be handled in a fume cupboard.Goggles, gloves and labcoats must be worn when handling stock or dilute solutions.Once cells/tissues have been fixed and washed, they may be treated as “normal” biological specimens outside the fume cupboard – but always wear gloves and labcoat.
ACCIDENT/SPILLAGE PROCEDURE
Within fume cupboard- clear spill with absorbent material and dispose of as hazerdous waste by the route described in 2.2.5Within main lab - use absorbent granules on spill and evacuate area
WASTE DISPOSALPlace is special container in fume cupboard- disposal is by contractor
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBBS. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF069
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Preparation of buffer solutions
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Tris- HClPhosphate buffered saline (PBS)
HEPES
N/AR22, R24/25
N/A
Harmful when swallowed, toxic in contact with skin and if swallowed
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good laboratory practice
ACCIDENT/SPILLAGE PROCEDURE
Good laboratory practice
WASTE DISPOSAL
All waste should be disposed of via the sink
S. Foster COSHH assessments Feb. 2014
COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)
DEPARTMENT: MBB S. FOSTER LABORATORY – F18
COSHH PROTOCOL NUMBER: 14SF070
ASSESSMENT CARRIED OUT BY:
S. FOSTER DATES FOR REASSESSMENT:
ANNUAL
PROJECT/WORK PROCESS:
Purification of proteins: glutathione and nickel agarose affinity chromatography
PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS
Nickel Agarose resin
Imidazole Glutathione sepharoseGlutathione
R10, R22, R40, R42/43
R22, R34N/A?N/A
Flammable; harmful when swallowed, limited evidence of carcinogenic effect, may cause sensitisation by skin contact or by inhalationHarmful when swallowed, causes burns
RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)
Good laboratory practice; wear gloves at all times
ACCIDENT/SPILLAGE PROCEDURE
Wipe area with water and detergent
WASTE DISPOSAL Place in clinical waste