Download - Home | The University of Sheffield - …/file/sample_C… · Web viewSpillages 1) Small spillages (< 25 ml) – soak up majority of the solution using absorbent “Blue Roll” towels

S. Foster COSHH assessments Feb. 2014

COSHH ASSESSMENTS

S. FOSTER LABORATORY

Every experimental procedure that involves chemicals and biological agents that present a potential hazard must be assessed for risk to human health. These assessments fall under the Control of Substances Hazardous to Health (COSHH) regulations.

Prior to conducting an experiment for the first time:

1. Consult Table 1 “Titles of COSHH assessments”

2. Look up the detailed assessment using the COSHH protocol number

3. Make sure you read and understand the assessment, paying particular attention to:

o RISK ASSESSMENT & CONTROL MEASURESo ACCIDENT/SPILLAGE PROCEDUREo WASTE DISPOSAL

4. If the procedure you intend to follow is not covered by these assessments, then you should conduct a risk assessment before you start. Do this using a blank form (see end of folder), and ensure that the completed form is typed up and added to the folder as soon as possible.

Notes:

Some techniques are covered by more than one protocol e.g. #s 19 and 18 cover SDS PAGE and sample preparation respectively

Risk Phrases can be found on the web sites of most manufactures’ web sites- their definitions are listed on page 2 - 4

If in doubt about how to write a COSHH assessment, contact S. Foster.

In the protocols, “Local Rules” refers to the risk assessments for handling ACDP hazard Group 2 pathogens and GMM class II organisms – WT2013_06 and GM2013_05.


ALL workers in the lab are informed about Coshh forms before starting in the lab.

DATE NAME UGS/PGS/PhD student or other

SIGNATURE


RISK PHRASES – PAGE 1R1 Explosive when dryR2 Risk of explosion by shock, friction, fire or other sources of ignitionR3 Extreme risk of explosion by shock, friction, fire or other sources of

ignitionR4 Forms very sensitive explosive metallic compoundsR5 Heating may cause an explosionR6 Explosive with or without contact with airR7 May cause fireR8 Contact with combustible material may cause fireR9 Explosive when mixed with combustible materialR10 FlammableR11 Highly flammableR12 Extremely flammableR14 Reacts violently with waterR14/15 Reacts violently with water, liberating extremely flammable gasesR15 Contact with water liberates extremely flammable gasesR15/29 Contact with water liberates toxic, extremely flammable gasesR16 Explosive when mixed with oxidising substancesR17 Spontaneously flammable in airR18 In use, may form flammable/explosive vapour-air mixtureR19 May form explosive peroxidesR20 Harmful by inhalationR20/21 Harmful by inhalation and in contact with skinR20/21/22 Harmful by inhalation, in contact with skin and if swallowedR20/22 Harmful by inhalation and if swallowedR21 Harmful in contact with skinR21/22 Harmful in contact with skin and if swallowedR22 Harmful when swallowedR23 Toxic by inhalationR23/24 Toxic by inhalation and in contact with skinR23/24/25 Toxic by inhalation, in contact with skin and if swallowedR23/25 Toxic by inhalation and if swallowedR24 Toxic in contact with skinR24/25 Toxic in contact with skin and if swallowedR25 Toxic if swallowedR26 Very toxic by inhalationR26/27 Very toxic and in contact with skinR26/27/28 Very toxic by inhalation, in contact with skin and if swallowedR26/28 Very toxic by inhalation and if swallowedR27 Very toxic in contact with skinR27/28 Very toxic in contact with skin and if swallowedR28 Very toxic if swallowedR29 Contact with water liberates toxic gasR30 Can become highly flammable in useR31 Contact with acids liberates toxic gasR32 Contact with acids liberates very toxic gasR33 Danger of cumulative effectsR34 Causes burnsR35 Causes severe burnsR36 Irritating to eyesR36/37 Irritating to eyes and respiratory systemR36/37/38 Irritating to eyes, respiratory system and skinR36/38 Irritating to eyes and skin


R37 Irritating to respiratory systemR37/38 Irritating to respiratory system and skinR38 Irritating to skinR39 Danger of very serious irreversible effectsR39/23 Toxic: danger of very serious irreversible effects through inhalationR39/23/24 Toxic: danger of very serious irreversible effects through inhalation and in

contact with skinR39/23/24/25 Toxic: danger of very serious irreversible effects through inhalation, in

contact with skin and if swallowedR39/23/25 Toxic: danger of very serious irreversible effects through inhalation and if

swallowedR39/24 Toxic: danger of very serious irreversible effects in contact with skinR39/24/25 Toxic: danger of very serious irreversible effects in contact with skin and if

swallowedR39/25 Toxic: danger of very serious irreversible effects if swallowedR39/26 Very Toxic: danger of very serious irreversible effects through inhalationR39/26/27 Very Toxic: danger of very serious irreversible effects through inhalation

and in contact with skinR39/26/27/28 Very Toxic: danger of very serious irreversible effects through

inhalation, in contact with skin and if swallowedR39/26/28 Very Toxic: danger of very serious irreversible effects through inhalation and

if swallowedR39/27 Very Toxic: danger of very serious irreversible effects in contact with skinR39/27/28 Very Toxic: danger of very serious irreversible effects in contact with skin and

if swallowedR39/28 Very Toxic: danger of very serious irreversible effects if swallowedR40 Limited evidence of a carcinogenic effectR40/20 Harmful: possible risk of irreversible effects through inhalationR40/20/21 Harmful: possible risk of irreversible effects through inhalation and in contact

with skinR40/20/21/22 Harmful: possible risk of irreversible effects through inhalation, in

contact with skin and if swallowedR40/20/22 Harmful: possible risk of irreversible effects through inhalation and if

swallowedR40/21 Harmful: possible risk of irreversible effects in contact with skinR40/21/22 Harmful: possible risk of irreversible effects in contact with skin and if

swallowedR40/22 Harmful: possible risk of irreversible effects if swallowedR41 Risk of serious damage to eyesR42 May cause sensitisation by inhalationR43 May cause sensitisation by skin contactR44 Risk of explosion if heated under confinementR45 May cause cancerR46 May cause heritable genetic damage


R48 Danger of serious damage to health by prolonged exposureR48/20 Harmful: danger of serious damage to health by prolonged exposure

through inhalationR48/20/21 Harmful: danger of serious damage to health by prolonged exposure

through inhalation and in contact with skinR48/20/21/22 Harmful: danger of serious damage to health by prolonged exposure

through inhalation, in contact with skin and if swallowedR48/20/22 Harmful: danger of serious damage to health by prolonged exposure

through inhalation and if swallowedR48/21 Harmful: danger of serious damage to health by prolonged exposure in

contact with skinR48/21/22 Harmful: danger of serious damage to health by prolonged exposure in

contact with skin and if swallowedR48/22 Harmful: danger of serious damage to health by prolonged exposure if

swallowedR48/23 Toxic: danger of serious damage to health by prolonged exposure

through inhalationR48/23/24 Toxic: danger of serious damage to health by prolonged exposure

through inhalation and in contact with skinR48/23/24/25 Toxic: danger of serious damage to health by prolonged exposure

through inhalation, in contact with skin and if swallowedR48/23/25 Toxic: danger of serious damage to health by prolonged exposure through

inhalation and if swallowedR48/24§ Toxic: danger of serious damage to health by prolonged exposure in contact

with skinR48/24/25 Toxic: danger of serious damage to health by prolonged exposure effects

in contact with skin and if swallowedR48/25 Toxic: danger of serious damage to health by prolonged exposure effects if

swallowedR49 May cause cancer by inhalationR50 Very toxic to aquatic organismsR50/53 Very toxic to aquatic organisms, may cause long-term adverse effects in

the aquatic environmentR51 Toxic to aquatic organismsR51/52 Toxic to aquatic organisms, may cause long-term adverse effects in the

aquatic environmentR52 Harmful to aquatic organismsR52/53 Harmful to aquatic organisms, may cause long-term adverse effects in

the aquatic environmentR53 May cause long-term adverse effects in the aquatic environmentR54 Toxic to floraR55 Toxic to faunaR56 Toxic to soil organismsR57 Toxic to beesR58 May cause long-term adverse effects in the environmentR59 Dangerous for the ozone layerR60 May impair fertilityR61 May cause harm to the unborn childR62 Possible risk of impaired fertilityR63 Possible risk of harm to the unborn childR64 May cause harm to breast-fed babiesR65 Harmful: may cause lung damage if swallowedR66 Repeated exposure may cause skin dryness or crackingR67 Vapours may cause drowsiness and dizziness


R68 Possible risk of irreversible effects


Table 1: Titles of COSHH assessments:

Protocols/experiments COSHH protocol number

Growth and handling of microorganisms, (HG1 and 2, including GMMs)

14SF044

Electroporation and transformation of bacteria 14SF045Preparation of protein samples 14SF046Staining bacterial cultures 14SF047Staining and immunostaining of cells 14SF048Approved disinfection methods 14SF049Preparation and handling of serum 14SF050Storage of cultures at -80oC or in liquid nitrogen 14SF051Agarose gel electrophoresis 14SF052Preparation of genomic and

plasmid DNA from bacteria 14SF053Extraction of DNA from Agarose gels 14SF054Recombinant DNA techniques- digestion, ligation, modification etc.

14SF055

SDS – PAGE 14SF056Preparation of samples for

PAGE (1D and 2D) 14SF057Western blotting – dry and wet 14SF058Antibody based detection of proteins on gels 14SF059ELISA 14SF060BCA protein assay 14SF061Coomassie blue staining 14SF062Ponceau staining 14SF063Extraction of proteins from gels and trypsin digestion 14SF064Glycosylation assay 14SF065Isoelectric focussing gel 14SF066Preparation and rp-HPLC analysis of peptidoglycan 14SF067

Use of glutaraldehyde for fixation of cells tissues 14SF068Preparation of buffer solutions 14SF069Purification of proteins: glutathione & nickel agaroseAffinity chromatography

14SF070


COSHH RISK ASSESSMENT FORM(To be completed for all processes involving hazardous substances)

DEPARTMENT: MBB S. FOSTER LABORATORY – F18

COSHH PROTOCOL NUMBER: 14SF044

ASSESSMENT CARRIED OUT BY:

S. FOSTER DATES FORREASSESSMENT:

ANNUAL

PROJECT/WORK PROCESS:

Growth and handling of microorganisms, including Hazard Group 1 and Hazard goup 2 organisms,including GMMs

PROTOCOL/SUBSTANCE RISK PHRASES

RISK STATEMENTS

Cultures of Enterococcus faecalis, Enterococcus faecium, Streptococcus pyogenes, Streptococcus suis, Streptococcus peumoniae, Bacillus cereus, Listeria monocytogenes (HG2), Staphylococcus aureusCultures of Escherichia coli and Lactococcus lactis (HG1)

AntibioticsKanamycin

Tetracyclin

Ampicillin

Neomycin

Chloramphenicol

Fusidic acid

Rifampicin

Gentamycin

Erythromycin

N/A

R61

R36/37/38

R36/37/38R42/43

R42/43

R45

R22

R22/36/37/38

R42/43

R42/43

N/A

May cause harm to the unborn childIrritating to eyes, respiratory system and skin

May cause sensitisation by inhalation or skin contact

May cause cancer

Harmful when swallowed

RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)See risk assessment GMO2015_05 for details concerning the organisms manipulated, control and measures.All antibiotics are weighed under the hood with appropriate PPE (gloves, labcoat); antibiotic solutions require gloves at all times.



RISK STATEMENTS

ACCIDENT/SPILLAGE PROCEDURE

Spillages1) Small spillages (< 25 ml) – soak up majority of the solution using absorbent “Blue Roll” towels and place these in an autoclave bag for disposal. Flood the area with 1% Virkon, leave for at least 10 minutes.Wipe area dry with absorbent towels, and place these in autoclave waste.

2) Larger (> 25ml spillages). a) Decontaminate area as in (a) above. b) Anything which cannot be decontaminated (paper, pencils etc.) must be discarded into the autoclave waste. c) Inform S. FOSTER d) REPORT THIS SPILL AS A DANGEROUS OCCURRENCE

WASTE DISPOSALSee risk assessment GMO2015_05 for details






S. FOSTER DATES FOR REASSESSMENT:

ANNUAL


Electroporation and transformation of bacteria

PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTS

E. coli culturesCalcium chloride; Use of electroporation apparatus (high voltage)

N/AN/AN/A

RISK ASSESSMENT & CONTROL MN/AEASURES (State if health surveillance required)

Good Laboratory Practice


Flood area with 1% Virkon. Mop up with paper towels and dispose in autoclave waste

WASTE DISPOSAL

Decontaminate HG1 bacterial cultures/supernatants with virkon 1% ; discard contaminated plasticware in biological waste.







ANNUAL


Preparation of protein samples

PROTOCOL/SUBSTANCE RISK PHRASE RISK STATEMENT

SDS

Glycine Urea TCA

Acetone

R20/22, R36/37/38, R42

N/AN/AR35-50/53

R11 R-36 R66/67

Harmful in contact with skin and if swallowed; irritating to eyes, respiratory system and skin; may cause sensitisation by inhalation

Causes severe burns. Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environmentEye Irritant. Repeated exposure may cause skin dryness/cracking. Vapour may cause dizziness.

RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)

SDS is an irritant in powder form. It is harmful when in contact with skin or when inhaled or swallowed. Wear gloves at all times. Wear face mask when weighing out powder

When using sonicator, make sure sound proof door is closed or that ear protection is wornGood Laboratory Practice


Culture spills: Flood area with 1% Virkon. Mop up with paper towels and dispose in autoclave wasteSDS spillage: wash area with water and detergent. If SDS is ingested or comes in contact with eyes, seek medical attention.

WASTE DISPOSALTCA must be disposed of as Special waste.Acetone in non-chlorinated waste solvent bottle







ANNUAL


Staining bacterial cultures


Gram's crystal violet

Gram's iodineGram's safranin Gram's Decolorizer (alcohol or acetone)

R22, R41, R45, R46, R50/53

N/AR41R10

Harmful when swallowed; risk of serious damage to eyes; may cause cancer and heritable genetic damage; very toxic to aquatic organisms, may cause long term adverse effects to the aquatic environment

Risk of serious damage to eyesFlammable

RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)All stains are potentially hazardous. Many are carcinogens, harmful if swallowed Alcohol and acetone are highly flammableGood Laboratory Practice

ACCIDENT/SPILLAGE PROCEDUREWash area with water and detergentIf affected, seek medical attention

WASTE DISPOSAL Waste stains and alcohol/acetone are washed down sink.Slides are placed in sharps boxBacteria are placed in autoclave waste







ANNUAL


Staining and immunostaining of cells


4% paraformaldehyde

bisBenzimide H 33258 (Hoescht stain)

R20/22, R34, R40, R43

22-36/38

Harmful by inhalation and if swallowed; causes burns; skin contact may cause sensitisation; limited evidence of carcinogenic effect. Harmful if swallowed, Irritating to the eyes, Irritating to the skin

RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)Paraformaldehyde: avoid contact or inhalation. Keep in a fume cupboard whenever possible

Hoescht stain: avoid contact, keep in fume cupboard when in powder form, make pup into solution and use that.



Spillage: wash affected area with water and detergentHoescht: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

WASTE DISPOSALWash down sink with copious amounts of water







ANNUAL


Approved disinfection methods


EthanolChlorosHycolin

Virkon

R11 R21/22R24/25, R34

R38, R41

Highly flammableHarmful in contact with skin or if swallowedToxic in contact with skin or if swallowed; causes burnsIrritating to skin, risk of serious damage to eyes.


Good Laboratory PracticeWear gloves at all times

If affected by exposure to Chloros or Hycolin, remove from exposure site and seek medical advice


Spillage: rinse affected site with water.

WASTE DISPOSALRinse waste down sinkPlace tissues etc in clinical waste.







ANNUAL


Preparation and handling of serum


Rodent or human blood and sera

N/A Human blood may carry infectious diseases.


Risk of contamination of human blood or sera with pathogens, e.g. HIV, hepatitis virus

Refer to the Protocol for “Handling ACDP Hazard Group Two Pathogens” for detailed protocol

Inactivate human sera by heating at 56oC for 60 minutes



Spillage: treat area with Virkon and discard towels as clinical wasteIn the event of any material entering the body in any way, e.g. through a cut, seek medical advice immediately.

WASTE DISPOSALDiscard in autoclave waste







ANNUAL


Storage of cultures at -80oC or in liquid nitrogen


Liquid NitrogenSolid CO2 (dry ice)

Hazardous due to burns and asphyxiation


Liquid nitrogen will freeze any tissue upon contact. Risk of eye injury if liquid enters eye.

Use only with thick gloves and a face shield.

Solid CO2 (dry ice) will cause burns on contact with skin. Always wear thick gloves. Risk of asphyxiation if large quantities of dry ice are kept in an enclosed area, e.g. a lift.



Liquid nitrogen and dry ice spillages: leave area and wait until evaporation occursBurns and other injuries –seek medical advice

WASTE DISPOSALThere is no designated waste disposal. Leave either chemical in a safe place to evaporate or sublime







ANNUAL


Agarose gel electrophoresis


Ethidium bromide

UV light

R22, R26, R36/37/38, R68

Harmful when swallowed; very toxic by inhalation; irritating to eyes, respiratory system and skin, possible risk of irreversible effectsHazardous

RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)Ethidium bromide is very toxic by inhalation, ingestion and skin contact. Causes skin/eye irritation. Irritating to mucous membranes & upper respiratory tract.

Avoid all skin contact or inhalation & observe GLP

Wear gloves. Never handle powder.

UV light is a powerful mutagen. Full-face UV protective face shield must be worn, and no skin should be exposed to UV light.



Spillage: Wipe up spill with towels – place in clinical wasteWash area with water and detergent. Seek medical advice if ethidium bromide is internalised or if concentrated solution contacts skin.

WASTE DISPOSALDiscard gel buffers down sink with copious amounts of waterPlace used gels in clinical waste.







ANNUAL


Preparation of genomic and plasmid DNA from bacteria


Plasmid prep kitsPhenol and phenol/chloroform

Lysozyme, mutanloysin

R20/22 R23/24/25, R34, R36, R45, R46

R23/24/25

Harmful by inhalation and if swallowedToxic by inhalation, contact with skin, or if swallowed; causes burns; irritating to eyes; may cause cancer or heritable genetic damage.Toxic by inhalation, contact with skin or if swallowed


Good Laboratory PracticeWhen using phenol or phenol/chloroform, always wear eye protection and gloves.


In case phenol solution is in contact with skin, wash with copious amounts of water and swab area with polyethylene glycol until odour has gone. If affected seek medical advice.

WASTE DISPOSALDiscard all solutions as autoclave waste except phenol/phenol chloroformPhenol / phenol chloroform – place in “chlorinated hydrocarbon waste” pot




COSHH PROTOCOLNUMBER: 14SF054



ANNUAL


Extraction of DNA from Agarose gels


Qiaex gel extraction kitBuffer QX1 Scalpels

R22 Harmful if swallowed


Buffer QX1 contains sodium perchlorate, a strong oxidising agent.

Avoid contact with strong acids, organics, fine powdered metals and reducing agents

Avoid contact with skin and eyes

Wear Safety glasses and gloves

Scalpels are sued to excise gel fragments. Handle with care.

Good Laboratory PracticeACCIDENT/SPILLAGE PROCEDURE

Remove from exposure, rinse affected site with water.If affected, seek medical advice

WASTE DISPOSALReagents – autoclave wasteScalpels – Cin Bins – do not keep for re-use







ANNUAL


Recombinant DNA techniques- digestion, ligation, modification etc.


DNA substrates, enzymes containing glycerol, buffers

N/A




Wipe area with water and detergent

WASTE DISPOSALPlace in autoclave waste







ANNUAL


SDS – PAGE


SDS

Acrylamide and bis-acrylamide

TEMED

R21/22, R36/37/38, R42

R23/24/25, R33, R45, R46

R11, R20/22, R34

Harmful in contact with skin and if swallowed; irritating to eyes, respiratory system and skin; may cause sensitisation by inhalationToxic by inhalation, in contact with skin and if swallowed, danger of cumulative effects, may cause cancer and heritable genetic damage.Carcinogen, mutagen, teratogenHighly flammable, harmful by inhalation and if swallowed, causes burns


Acrylamide is highly toxic and is easily absorbed by the skin

TEMED is highly flammable, irritant

Use mask when weighing out solid SDS Good Laboratory Practice

Wear safety glasses and gloves



WASTE DISPOSALUnpolymerised acryalamide – polymerise with TEMED and ammonium persulphate and treat as clinical wastePolymerised gels - clinical waste







ANNUAL


Preparation of samples for PAGE (1D and 2D)

PROTOCOL/SUBSTANCE RISK PHRASE RISK PHRASE

SDS

DTT

mercaptoethanol

R21/22, R36/37/38, R42

R22, R36/37/38

R22, R24, R34, R51/53

Harmful in contact with skin and if swallowed; irritating to eyes, respiratory system and skin; may cause sensitisation by inhalationHarmful if swallowed; irritant to eyes, respiratory system and skinHarmful if swallowed; toxic in contact with skin; causes burns; toxic to aquatic organisms and may cause long term adverse effects in the aquatic environment.


Good Laboratory Practice mercaptoethanol - do not breath vapour. Handle stock solution in the fume cupboardSDS - powder is an irritant. Wear mask when weighing outGood laboratory practice


Wash area with water and detergent. Seek medical advice if affected.

WASTE DISPOSAL

Small quantities (microlitres) in autoclave waste; larger quantities dispose by sink with copious amounts of water







ANNUAL


Western blotting – dry and wet


Methanol

Acetic acid

R11, R39/23/24/25

R10, R35

Highly flammable. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowedFlammable, causes severe burns



Wipe spills with tissues, water and detergent

WASTE DISPOSAL

Discard reagents down sink







ANNUAL


Antibody based detection of proteins on gels


ECL reagents 1 and 2Antibody conjugatesPrimary antibodies

N/AN/AN/A

No known hazardNo known hazardIf human, potentially could contain viruses


Good Laboratory PracticeIf antibodies used are human origin, refer to Local Rules before starting workAll human sera must be heat treated prior to handling


Wash area with water and detergent

WASTE DISPOSAL

Place gels in clinical waste; solutions down sink with excess water







ANNUAL


ELISA


Primary antibodyAntibody conjugatesOPD

citric acid30% hydrogen peroxide solution3M sulphuric acid (16%)

N/AN/AR20/21, R25, R36, R40, R43, R50/53, R68

R41R34

R35

No known hazards, but if human may contain virusesNo known hazardsHarmful by inhalation and in contact with skin, toxic if swallowed, irritating to eyes, limited evidence of a carcinogenic effect, may cause sensitisation by skin contact, very toxic to aquatic organisms, may cause long term adverse effects to the aquatic environmentRisk of serious damage to eyesCauses burns

Causes severe burns



WASTE DISPOSALPlace in autoclave waste




COSHH PROTOCOL NUMBER:14SF070



ANNUAL


Purification of proteins: glutathione and nickel agarose affinity chromatography


Nickel Agarose resin

Imidazole Glutathione sepharoseGlutathione+ other things ??

R10, R22, R40, R42/43

R22, R34N/A?N/A

Flammable; harmful when swallowed, limited evidence of carcinogenic effect, may cause sensitisation by skin contact or by inhalationHarmful when swallowed, causes burns


Good Laboratory Practice , wear gloves



WASTE DISPOSALPlace in clinical waste







ANNUAL


BCA protein assay


NaCO3; NaHCo3 Na tartrate NaOH Bicinchoninic acid CuSO4

BSA in 0.05% Na azide

R36R35R36/37/38R22, R36/38R28, R23

Irritating to eyesCauses severe burnsIrritating to eyes, respiratory system and skinHarmful when swallowed; irritating to eyes and skinVery toxic if swallowed, toxic by inhalation


Good laboratory practice

Wear glovesACCIDENT/SPILLAGE PROCEDURE


WASTE DISPOSALPlace small amounts in clinical waste; large amounts wash down sink with copious amounts of water







ANNUAL


Coomassie blue staining


Coomassie brilliant blueMethanol

Acetic acid

N/AR11, R39/23/24/25

R10, R35

Highly flammable. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowedFlammable, causes severe burns


Good Laboratory Practice.


Wipe area with waterIf ingested or inhaled, seek immediate medical attention

WASTE DISPOSALPour solutions down sink with copious amounts of water







ANNUAL


Ponceau staining


Acetic acid

Ponseau S

R10, R35

R36/37/38, R51/53

Flammable, causes severe burns

Irritating to eyes, respiratory system and skin; toxic to aquatic organisms and may cause long-term adverse effects in the aquatic environment




Wipe area with water

WASTE DISPOSALPour solutions down sink with copious amounts of water

http://www.serva.de/products/info/R_51_53.shtml

http://www.serva.de/products/info/R_36_37_38.shtml







ANNUAL


Extraction of proteins from gels and trypsin digestion


Tris, EDTA, NaCl, MgCl

MnCl

Sodium deoxycholate

Urea

R36/37/38

R22, R36/37/38, R40

R22, R52

N/A

Irritating to eyes, respiratory system and skin

As above plus harmful when swallowed, limited evidence of carcinogenic effect

Harmful if swallowed; harmful to aquatic life





WASTE DISPOSALPour solutions down sink with copious amounts of water; place gel fragments in clinical waste







ANNUAL


Glycosylation assay


RISK STATEMENTS

Sodium-meta-perjodate

Sodium metabisulfite

N,N-Dimethylformamide

Digoxigenin-3-O-succinyl-amidocapron acid-hydrazid-hydro-chloride

4-Nitro blue tetrazolium chloride

Acetic acid

Ponceau S

R7, R20/22

R22, R31, R36/37, R41

R61, R20/21, R36

R26/27/28, R33, R36

R20/21/22

R10, R35

R36/37/38

May cause fire, harmful by inhalation or if swallowed

Harmful when swallowed, contact with acids liberates toxic gas, irritating to eyes and respiratory system, risk of serious damage to eyes

May cause harm to the unborn child, harmful by inhalation and in contact with skin, irritating to eyes

Very toxic by inhalation, in contact with skin or if swallowed, danger of cumulative effects; irritating to eyes

Harmful by inhalation, if swallowed or in contact with skin

Flammable,causes severe burns

Irritating to eyes, respiratory system and skin

RISK ASSESSMENT & CONTROL MEASURES (State if health surveillance required)Good laboratory practice

Although small amounts are used, take extreme care.


Wipe area with water and detergentSeek medical attention if affected

WASTE DISPOSALPlace in clinical waste







ANNUAL


Isoelectric focussing gel


Tris

CHAPS bufferUrea

R36/37/38

R36/37/38 N/A

36/37/38: Irritating to eyes, respiratory system and skin





WASTE DISPOSALPlace gels in clinical wasteLiquid waste should be washed down sink with excess water







ANNUAL


Preparation and rp-HPLC analysis of peptidoglycan

PROTOCOL/SUBSTANCE RISK PHRASES RISK STATEMENTSAmmonium phosphateTCATFA

Methanol

Acetonitrile

Na-azide

N/AR35-50/53R20/R35/R52/53

R11, R39/23/24/25

R11-R23/24/25

R28, R23

N/ACauses severe burns. Highly corrosive

Highly flammable. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowedHighly flammable (flash point +2°C, Toxic

Very toxic if swallowed, toxic by inhalation


Good laboratory practiceAll buffers containing TFA, Methanol and acetonitrile should be prepared under the hood, and transferred into sealed bottles to avoid inhalation.



WASTE DISPOSAL

TCA must be disposed of as Special waste.Methanol volumes less than 50ml are disposed of in the sinkDispose of large amounts of HPLC buffer containing diluted chemicals (wash down sink with copious amounts of water)

http://en.wikipedia.org/wiki/R52/53:_Harmful_to_aquatic_organisms,_may_cause_long-term_adverse_effects_in_the_aquatic_environment

http://en.wikipedia.org/wiki/R35:_Causes_severe_burns

http://en.wikipedia.org/wiki/R20:_Harmful_by_inhalation







ANNUAL


Use of glutaraldehyde in scanning EM and fixation of cells tissues


Glutaraldehyde R20/21/22R36/37R42

Harmful by inhalation, in contact with skin and if swallowedIrritating to eyes and respiratory systemMay cause sensitization by


Glutaraldehyde is toxic and must be used with extreme care. Allergy due to sensitisation is common.It must only be handled in a fume cupboard.Goggles, gloves and labcoats must be worn when handling stock or dilute solutions.Once cells/tissues have been fixed and washed, they may be treated as “normal” biological specimens outside the fume cupboard – but always wear gloves and labcoat.


Within fume cupboard- clear spill with absorbent material and dispose of as hazerdous waste by the route described in 2.2.5Within main lab - use absorbent granules on spill and evacuate area

WASTE DISPOSALPlace is special container in fume cupboard- disposal is by contractor



DEPARTMENT: MBBS. FOSTER LABORATORY – F18




ANNUAL


Preparation of buffer solutions


Tris- HClPhosphate buffered saline (PBS)

HEPES

N/AR22, R24/25

N/A

Harmful when swallowed, toxic in contact with skin and if swallowed





WASTE DISPOSAL

All waste should be disposed of via the sink







ANNUAL


Purification of proteins: glutathione and nickel agarose affinity chromatography


Nickel Agarose resin

Imidazole Glutathione sepharoseGlutathione

R10, R22, R40, R42/43

R22, R34N/A?N/A

Flammable; harmful when swallowed, limited evidence of carcinogenic effect, may cause sensitisation by skin contact or by inhalationHarmful when swallowed, causes burns


Good laboratory practice; wear gloves at all times



WASTE DISPOSAL Place in clinical waste

Download - Home | The University of Sheffield - …/file/sample_C… · Web viewSpillages 1) Small spillages (< 25 ml) – soak up majority of the solution using absorbent “Blue Roll” towels

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