Sugar Sensitive Phenylboronic Acid-Based Hydrogels: Towards glucose analysis in sweat

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HENOK TESFAY 4th year project presentation

Supervisors: Dr. Aoife Morrin,

Dr Larisa Elena Florea Dr. Colm Delaney

School of Chemical Sciences May 2016

Phenylboronic acid (PBA)

PBA bearing hydrogel

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Sugar

Photochemical polymerization(Gel forming reagents)

Acrylamide(AAm)

linker

3-(Acrylamido) phenylboronic acid – (PBA)

sugar reactive monomer

2-Hydroxy-2-methpropiophene-(HMPP)

photo initiator

Methylenebisacrylamide (BIS)

Cross-linker

3PolyDimethylsiloxane(PDMS) mould

Uvp CL 1000 ultraviolet crosslinker

Source: http://www. idf.org/about-diabetes [1] 4

Diabetes is becoming a big problem in our society, affecting currently one in every 20 people in the world.[1]

Prevalence of Diabetes • Diabetes caused 5.1 million

deaths in 2013. [1]• Every six seconds a person

dies from diabetes.[1]

Source: http://www. idf.org/about-diabetes [1]

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Is there a cure for Diabetes?• No cure for diabetes • Can be controlled but not

easy. • Must prick their finger to

get drops of blood for test. • Limitation – don’t know

blood glucose between checks?

• Need advanced sensor

Source: http://www.slideshare.net/funk97 / glucose-monitoring-smart-watch

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• Non-invasive *Blood free * not causing pain or trauma *real-time glucose sensors. *Ables to monitor during the day and night *use sweat, saliva or tears .

Source: http://ingenuitylab.ca/about

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Source: Health Tech Insider

Time dependent swelling of Hydrogel in 10mM Fructose solution (5mol% PBA)

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gravimetric method

Behaviour of 5mol% PBA Hydrogel in Glucose, Sucrose & Fructose Solutions

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Behaviour of Hydrogel (20mol% PBA) in different concentration of Glucose, Fructose & Sucrose

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20mol%PBA gel in Glucose

• Control

• In 4mM

• In 6mM

• In 10mM

• In 20 mM

20mol%PBA gel in Fructose

•Control

• In 4mM

• In 6mM

• In 10mM

• In 20mM

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Linear regression Analysis of Calibration Plots

Sugars R2 (1-5mM) Slope (Swelling ratio/[]

LOD (3σ/M) LOQ(10σ/M)

Fructose 0.9971 0.067 0.30 1.0

Glucose 0.9933 0.026 0.50 1.50

Sucrose 0.8661 0.003 2.20 7.22

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Glucose in 20mol% & 5mol% PBA Hydrogel

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Conclusion

• The hydrogel shown a swelling in response to the Glucose and Fructose. But didn’t show a response to the sucrose.

• Challenges: Accuracy, the PBA has more sensitive for fructose than glucose

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Future work

• Measuring using impedance. • Adding tertiary amine

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fixed voltage 20 mV frequency range 0.1 – 10 MHz

Solartron (Impedance)

0 5 10 15 20 25 30 35 40 45 500

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2

3

4

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C (n

F)

glucose concentration (mM)

1 kHz

Acrylamide20 mol % PBA1 mol % bisAm1mol % HMPP(1:1) water/DMSO

UV light30 minutes

Electrode Functionalization with Hydrogel

Glucose Additions

Electrical Impedance Spectroscopy

Source: Insight centre for Data analytics (DCU)

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