Ghost Pulses Eric Baril

Mr. Barnard3rd

Due: Dec. 10, 2013

Essential Question

• Do different energy drinks, the ingredients in the energy blend of the 5 Hour Energy Drink, and combinations of those ingredients have an effect on the heart rate of a larger species such as Palaemantes Paludousus? And what are they?

Purpose

• My past two years worth of science projects have been testing the heart rates of Daphnia Magna. This year I’m using a different organism known as the Ghost shrimp. These are mush larger than the Daphnia and might have different results. I wanted to use a larger organism because the point of my projects are to research how these drinks will affect humans. Since Daphnia are so small you need a microscope to see them the comparison is hard to grasp. Also This Project is to verify the fact that energy drinks can harm humans. By using a larger organism we can solidify the Fact.

Research

Heart

Research

Chemical Compounds in 5 Hour Energy Drink energy blend• Taurine• Tyrosine• Caffeine• Phenylalanine• Glucuronolactone• Malic acid • Choline

Research

• In 2011, sales of the beverage category of “energy shots” rose to 1 billion dollars.• 5 hour leads the category with increased sales of 45.3%, which

yielded 885.2 million dollars.• The energy drinks category increased 15.7% yielding 6.9 billion dollars

for the 52 weeks prior to May 15, 2011.• Share of volume by region in the U.S. in 2008 : West-42.4%, South-

27.8%, Midwest-18.9%, North East-10.8%.

ResearchThe Ghost Shrimp:• Females are larger than males• Also called glass shrimp because they are

clear• Type of feeder shrimp often used in

larger aquariums to Clean the tank or as fish food

• Maximum size is two inches• Palemontes Paludousus

Hypothesis

• I hypothesize that the ingredients and the different drinks will have the same effect on the glass shrimp as they did on daphnia magna. • I feel that the ingredients will combine to have a larger effect on the

shrimp. I feel the more ingredients added to the compound the larger the effect they will have.

Materials

• Palaemantes Paludousus• Water • Medicine Droppers• Smart Phone • Laser pointer• Water • Taurine• Tyrosine

• Caffeine• Phenylalanine • 5 Hour Energy Drink• Monster• Amp• Red Bull• Nos • Small three inch fish net

• Super glue• Scotch tape• Flash light• Soda bottle • Metal cutters • Hammer • Scissors

Compounds• Taurine-Tyrosine• Taurine-Phenylalanine • Taurine-Caffeine• Tyrosine-Phenylalanine • Tyrosine-Caffeine • Caffeine-Phenylalanine• Taurine-Tyrosine-Phenylalanine• Tyrosine-Phenylalanine-Caffeine • Phenylalanine-Caffeine-Taurine• Caffeine-Taurine-Tyrosine • Taurine-Tyrosine-Phenylalanine-Caffeine

Procedure

1. Use the cutters to cut the outer tube of the laser pointer so you now have a piece of metal with two holes in it.

2. Remove the circuit board from the laser tubing .3. Open the tube carefully as to not break the lens.4. Remove the lens.5. Take the outer tubing an flatten it so you have a flat piece of

metal with two holes. 6. Put the lens in the holes and secure it with super glue. 7. Tape the piece of metal with the lens in side to the camera

on your phone.8. Now take your soda bottle and cut of the neck of the bottle.9. Cut it down to the lip right before the cap.10. Remove the ring around the cap.11. Put the cap on.

Procedure (continued)

1. Remove one shrimp with the net and put him in the cap with a little of its own water.

2. Use your smart phone camera to count the heart beats for one minute.

3. Add a drop of a substance in the water.4. Wait one minute. 5. Count the beats for one minute. 6. Repeat steps 1-5 For each trial for each substance making sure to

record your data after you count each time.7. Do this for the different energy drinks, ingredients, and compounds.

Graph (tyrosine)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 104 112 156 164 188 196

Trial 2 112 130 152 178 180 194

Trial 3 122 138 164 172 186 184

Average 122.66 126.66 157.33 171.33 184.66 191.33

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75

125

175

225

Trial 1 Trial 2 Trial 3 Average

Graph (Taurine)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 126 162 166 182 196 210

Trial 2 124 166 192 204 216 224

Trial 3 150 178 202 212 222 220

Average 133.333333333333 168.666666666667 186.666666666667 199.333333333333 211.333333333333 218

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225

Trial 1 Trial 2 Trial 3 Average

Graph (phenylalanine)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 144 182 186 194 202 202

Trial 2 154 182 196 204 208 210

Trial 3 170 186 200 224 224 208

Average 156 183.333333333333 194 207.333333333333 211.333333333333 206.666666666667

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75

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175

225

Trial 1 Trial 2 Trial 3 Average

Graph (caffeine)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 174 196 242 236 0 0

Trial 2 178 210 240 236 12 6

Trial 3 172 202 238 230 100 14

Average 174.666666666667 202.666666666667 240 234 37.3333333333333 6.66666666666667

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Trial 1 Trial 2 Trial 3 Average

Graph (5 Hour-control)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 174 206 244 254 182 112

Trial 2 178 216 248 258 160 120

Trial 3 182 248 262 180 0 0

Average 178 223.3333333 251.3333333 230.6666667 114 77.33333333

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Trial 1 Trial 2 Trial 3 Average

Graph (ingredients compilation)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Tyrosine 122.66 126.66 157.33 171.33 184.66 191.33

Taurine 133.33 168.66 186.66 199.33 211.33 218

Phenylalanine 156 183.33 194 207.33 211.33 206.66

Caffeine 174.66 202.66 240 234 37.33 6.66

5-Hour (control) 178 223.33 251.33 230.66 114 77.3

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Tyrosine Taurine Phenylalanine Caffeine 5-Hour (control)

Graph (Monster)

no drop 1 drop 2 drops 3 drops 4 drops 5 drops

Trial 1 166 174 196 226 250 268

Trial 2 176 192 216 248 136 54

Trial 3 156 184 214 242 202 178

average 166 183.333333333333 208.666666666667 238.666666666667 196 166.666666666667

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Trial 1 Trial 2 Trial 3 average

Graph (Amp)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 152 182 212 188 150 108

Trial 2 170 206 252 264 296 322

Trial 3 154 170 194 216 234 252

Average 158.666666666667 186 219.333333333333 222.666666666667 226.666666666667 227.333333333333

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325

Trial 1 Trial 2 Trial 3 Average

Graph (red bull)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 174 200 228 250 274 280

Trial 2 164 194 214 246 264 270

Trial 3 142 162 178 192 218 224

Average 160 185.333333333333 206.666666666667 229.333333333333 252 258

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Trial 1 Trial 2 Trial 3 Average

Graph (nos)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 182 226 250 270 294 304

Trial 2 138 150 178 186 214 244

Trial 3 150 176 194 218 228 254

Average 156.666666666667 184 207.333333333333 224.666666666667 245.333333333333 267.333333333333

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Trial 1 Trial 2 Trial 3 Average

Graph (5 Hour)

No Drops Drop 1 Drop 2 Drop 3 Drop 4 Drop 5

Trial 1 174 206 244 254 182 112

Trial 2 178 216 248 258 160 120

Trial 3 182 248 262 180 0 0

Average 178 223.3333333 251.3333333 230.6666667 114 77.33333333

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Trial 1 Trial 2 Trial 3 Average

Graph (drinks compilation)

no drops 1 drop 2 drops 3 drops 4 drops 5 drops

Monster 166 183.3333333 208.6666667 238.6666667 196 166.6666667

Amp 158.6666667 186 219.3333333 222.6666667 226.6666667 227.3333333

Red Bull 160 185.3333333 206.6666667 229.3333333 252 258

Nos 156.6666667 184 207.3333333 224.6666667 245.3333333 267.3333333

5 Hour 178 223.3333333 251.3333333 230.6666667 114 77.33333333

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Monster Amp Red Bull Nos 5 Hour

Compounds (trial 1)

0 drops 1 drop 2 drops 3 drops 4 drops 5 drops

Ta Ty 150 192 208 234 246 258

Ta Ph 162 194 222 250 264 294

Ta Ca 164 202 225 250 228 192

Ty Ph 142 182 220 254 276 302

Ty Ca 146 192 236 262 294 304

Ca Ph 168 186 214 240 228 202

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Ta Ty Ta Ph Ta Ca Ty Ph Ty Ca Ca Ph

Compounds (trial 1)

no drops 1 drop 2 drops 3 drops 4 drops 5 drops

Ta Ty Ph2 136 186 234 256 278 336

Ty Ph Ca 150 182 222 232 198 162

Ph Ca Ta 156 192 228 252 262 228

Ca Ta Ty 162 208 232 254 180 120

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375

Ta Ty Ph2 Ty Ph Ca Ph Ca Ta Ca Ta Ty

Compounds (trial 2)

0 drops 1 drop 2 drops 3 drops 4 drops 5 drops

Ta Ty 152 194 210 236 246 254

Ta Ph 164 196 224 252 264 284

Ta Ca 166 204 226 256 228 188

Ty Ph 144 184 222 256 276 304

Ty Ca 148 190 238 264 296 306

Ca Ph 170 188 216 242 228 206

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Ta Ty Ta Ph Ta Ca Ty Ph Ty Ca Ca Ph

Compounds (trial 2)

no drops 1 drop 2 drops 3 drops 4 drops 5 drops

Ta Ty Ph 134 184 228 254 278 334

Ty Ph Ca 150 180 220 230 198 166

Ph Ca Ta 148 190 226 248 262 238

Ca Ta Ty 160 206 230 252 180 124

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Ta Ty Ph Ty Ph Ca Ph Ca Ta Ca Ta Ty

Complete compound with energy drink compilation.

no drops 1 drop 2 drops 3 drops 4 drops 5 drops

Monster 166 183.3333333 208.6666667 238.6666667 196 166.6666667

Amp 158.6666667 186 219.3333333 222.6666667 226.6666667 227.3333333

Red Bull 160 185.3333333 206.6666667 229.3333333 252 258

Nos 156.6666667 184 207.3333333 224.6666667 245.3333333 267.3333333

5 Hour 178 223.3333333 251.3333333 230.6666667 114 77.33333333

complete trial 1 166 216 114 87 47 0

complete trial 2 164 214 244 124 86 0

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Monster Amp Red Bull Nos 5 Hour complete trial 1 complete trial 2

Conclusion

• In Conclusion it would appear that my Hypothesis was correct and the ingredients did in fact effect the ghost shrimp. This just goes to show that energy drinks do in fact have negative affects on humans. I do believe there is a good purpose for energy drinks they just haven’t found it yet.• My hypothesis was also correct that the drinks and ingredients had

the same affect on the shrimp. This just goes to show that these substances will harm people and that they are capable of great damage to the human body.

Bibliography• http://www.engineeringtoolbox.com/water-density-specific-weight-d_595.html• http://www.webmd.com/vitamins-supplements/ingredientmono-979-CAFFEINE.aspx?activeIngredientId=979&activeIngre

dientName=CAFFEINE• ©2005-2012 WebMD, LLC. All rights reserved.• http://www.webmd.com/vitamins-supplements/ingredientmono-1090-CITICOLINE.aspx?activeIngredientId=1090&activeIn

gredientName=CITICOLINE• http://www.webmd.com/vitamins-supplements/ingredientmono-1037-TYROSINE.aspx?activeIngredientId=1037&activeIng

redientName=TYROSINE• http://www.webmd.com/vitamins-supplements/ingredientmono-653-PHENYLALANINE.aspx?activeIngredientId=653&activ

eIngredientName=PHENYLALANINE• http://www.webmd.com/vitamins-supplements/ingredientmono-1024-TAURINE.aspx?activeIngredientId=1024&activeIngr

edientName=TAURINE• http://www.webmd.com/vitamins-supplements/ingredientmono-977-Malic%20Acid%20(ALPHA%20HYDROXY%20ACIDS).a

spx?activeIngredientId=977&activeIngredientName=Malic%20Acid%20(ALPHA%20HYDROXY%20ACIDS)• GLUCURONOLACTONE http://www.bodybuilding.com/fun/jrod15.htm• http://www.liveaquaria.com/product/prod_display.cfm?c=1075+1468&pcatid=1468

Phone Microscope Example

Pictures

• Glass shrimp through camera • Glass shrimp in bag

Picture• Microscope from the outside

Pictures • Set up