# genome rearrangement by ghada badr part i

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Genome, chromosome, gene, gene order The entire complement of genetic material carried by an individual is called the genome. Each genome contains one or more DNA molecules, one per chromosomeTRANSCRIPT

Genome Rearrangement By Ghada Badr Part I Genome, chromosome, gene, gene order

The entire complement of genetic material carried by an individual is called the genome. Each genome contains one or more DNA molecules, one per chromosome Genome, chromosome, gene, gene order

A gene is a segment of DNA sequence with a specific function Genome, chromosome, gene, gene order

A C D F 5 3 3 5 B E Gene order:A -B C D EF Genes can be ordered by their DNA sequence location. DNA consists of two complementary strands twisted around each other to form a right-handed double helix. A sign (+/-) is usually used to indicate on which strand a gene is located. Genome, chromosome, gene, gene order

A B C D E F H I K J The DNA molecule (chromosome) may be circular or linear Genome Rearrangement A -B C D -E F B -E F -D A C

The genome is structurally specific to each species, and it changes only slowly over time. Therefore genome comparison among different species can provide us with much evidence about evolution. Genome rearrangements are an important aspect of the evolution of species. Even when the gene content of two genomes is almost identical, gene order can be quite different. A -B C D -E F Genome 1 B -E F -D A C Genome 2 Genome Rearrangement Gene order analysis on a set of organisms is a powerful technique for genomic comparison phylogenetic inference. Genome Rearrangement General Definition for the problem:

Given a set of genomes and a set of possible evolutionary events (operations), find a shortest set of events transforming (sorting) those genomes into one another. What genome means and what events are, makes the diversity of the problem. Since these events are rare, scenarios minimizing their number are more likely close to reality. Many models have been proposed. Genome Models Genes (or blocks of contiguous genes) are a good example of homologous markers, segments of genomes, that can be found in several species. The simplest possible model is: The order of genes in each genome is known, All the genomes share the same set of genes, All genomes contain a single copy of each gene, and All genomes consist of a single chromosome. Genome Models Genomes can be modeled by each gene can be assigned a unique number and is exactly found once in the genome. permutations: Signed Permutation: Each gene may be assigned + or - sign to indicate the strand it resides on. Unsigned Permutation: If the corresponding strand is unknown. Permutaions Genes (markers) are represented by integers:

with +,- sign to indicate the strand they lie on. The order and orientation of genes of one genome in relation to the other is represented by a signed permutation . = ( 2 n-1 n) of size n over {-n, ... , -1, 1, ... , n}, such that for each i from 1 to n, either i or -i is mandatory represented, but not both. Permutaions Identity permutation:

The identity permutation n = (1, 2, 3, , n). When multiple genomes with the same gene content are compared, one of them is chosen as a base (reference), i.e, represented as n, and all other identical genes are given the same integer values. Permutaions Sorted/unsorted permutation:

In order to sort a permutation this means that we want to apply some operations on to change it to n. If (1 = 2) We say that is sorted with respect to . If (1 2) We say that is unsorted with respect to . Permutaions Example: Mitochondrial Genomes of 6 Arthropoda

1= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) Fruit Fly Mosquito Silkworm Locust Tick Centipede 2= (1 , 2 ,3 , 4,5,6,8,7,9,-10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 3= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 14 , 13 , 15 , 16 , 17) 4= (1 , 2 ,3 , 5,4,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 5= (1 , 3 , 4,5,6,7,8,9, 10 ,11 ,-2 , 12 , 13, 14 , 15 , 16 , 17) 6= (1 , 3 , 4,5,6,7,8,9, 10 , 11 , -2 , 12 , 16 , 13 , 14 , 15 , 17) Permutaions Example: Mitochondrial Genomes of 6 Arthropoda

1= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) Fruit Fly Mosquito Silkworm Locust Tick Centipede 2= (1 , 2 ,3 , 4,5,6,8,7,9,-10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 3= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 14 , 13 , 15 , 16 , 17) 4= (1 , 2 ,3 , 5,4,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 5= (1, 3 , 4,5,6,7,8,9, 10 ,11 ,-2 , 12 , 13, 14 , 15 , 16 , 17) 6= (1 , 3 , 4,5,6,7,8,9, 10 , 11 ,-2 , 12 , 16 , 13 , 14 , 15 , 17) Permutaions Linear and circular permutation:

is linear when it represents a linear chromosome, or circular when it represents a circular chromosome. When = ( 2 n-1 n) is circular: = (-n n-1 2 1) all permutations obtained by shifts on or shift( , i) = (n-i+1 n-i+2n-1 n1 n-i are all equivalent. Example: (-3,2,1,-4) & (-1,-2,3,4) Permutaions Points in permutations

For a given permutation = ( 2 n-1 n), there is a point between each pair of consecutive values i and i+1 in . If is linear: there are two additional points, one before and one after n. If is circular: there is one additional point between nand 1. Pts() = n+1 if linear, and pts() = n if circular. Permutaions Linear extension of a permutation:

For a given = ( 2 n-1 n) If is linear: a linear extension of is = (0, 2 n-1 n, n+1) If is circular: a linear extension of is = (0, 2 n-1 n-1, n) Permutaions Example: = (4,8,9,7,6,5,1,3,2)

= (0,4,8,9,7,6,5,1,3,2,10) = ( ) Then Pts() = 10 Now: we want to compare our genomes. Permutations - similarity/distance

Problem: Given two genomes, How do we measure their similarity and/or distance? A Related Problem: Given two permutations, How do we measure their similarity and/or distance? Permutations - similarity/distance

A distance measure should be a metric on the set of genomes. A Metric d on a set S (d: S S R) satisfies the following three axioms: Positivity: for all s, t in S, d(s,t) 0, and d(s,t)=0 iff s = t. Symmetry: for all s, t in S, d(s,t) = d(t,s). Triangular inequality: for all s, t, u in S, d(s,u) d(s,t) + d(t,u). Permutations - similarity/distance

Measures of similarity between permutations that are used in computational biology are numerous in literature. First measures used are (will be useful later on): Breakpoints (Introduced by Sankoff and Blanchette (1997)) Common intervals Permutations-distance - Breakpoints

When analyze with respect to , each point in can be an adjacency or a breakpoint. A point (pair of consecutive values) (i, i+1) in is an adjacency between and : when either (i, i+1) or (-I+1, -i) are consecutive in . If is linear: we have adjacency before if is also the first value in , and an adjacency after n, if n is also last value in . If is circular: we assume that n is also last value in and (n, 1) is an adjacency if is also the first value in . Permutations-distance - Breakpoints

brp() = pts() - adj() where: pts() is the number of points in . adj() is the number of adjacencies. If is sorted ( = ): has only adjacencies and no breakpoints (brp() = 0). If is unsorted ( ): has at least onebreakpoint (brp() 0). Breakpoint distance counts the lost adjacencies between genomes. The breakpoint distance between and is: Permutations-distance - Breakpoints

Back to our Example: = (4,8,9,7,6,5,1,3,2) = (0,4,8,9,7,6,5,1,3,2,10) = ( ) Then Pts() = 10, brp()? Adjacencies? n= ( ) (8,9) (7,6) (6,5) (3,2) adj() = 4 brp() = pts() - adj() = = 6 Permutations-distance - Breakpoints

Breakpoint distance is based on the notion of conserved adjacencies and can be defined on a set of more than two genomes. It is easy to compute. It always fails to capture more global relations between genomes. The first generalization of adjacencies is the notion of common intervals. Permutations-distance - Common Intervals

Common intervals: subsets of genes that appear consecutively together in two or more genomes, where genes are the same in each interval but may be not in the same order or orientation. Example (circular chromosomes) 1= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 2= (1 , 2 ,3 , 4,5,6,8,7,9,-10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 3= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 14 , 13 , 15 , 16 , 17) 4= (1 , 2 ,3 , 5,4,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 5= (1 , 3 ,4 , 5,6,7,8,9, 10 , 11 ,-2 , 12 , 13 , 14 , 15 , 16 , 17) 6= (1 , 3 ,4 , 5,6,7,8,9, 10 , 11 ,-2 , 12 , 16 , 13 , 14 , 15 , 17) If compare the first 4 species: they share 6 adjacencies {1,2}, {2,3},{11.12},{15,16},{16,17},{17,1} If compare all 6 species: they share only 1 adjacency {17,1} Permutations-distance - Common Intervals

Common intervals: subsets of genes that appear consecutively together in two or more genomes, where genes are the same in each interval but may be not in the same order or orientation. Example (circular chromosomes) 1= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 2= (1 , 2 ,3 , 4,5,6,8,7,9,-10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 3= (1 , 2 ,3 , 4,5,6,7,8,9, 10 , 11 , 12 , 14 , 13 , 15 , 16 , 17) 4= (1 , 2 ,3 , 5,4,6,7,8,9, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17) 5= (1 , 3 ,4 , 5,6,7,8,9, 10 , 11 ,-2 , 12 , 13 , 14 , 15 , 16 , 17) 6= (1 , 3 ,4 , 5,6,7,8,9, 10 , 11 ,-2 , 12 , 16 , 13 , 14 , 15 , 17) The six permutations are very similar. The genes in the interval [1,12] are all the same, as genes in the intervals [3,6], [6,9],[9,11], and [12,17]. Permutations-distance - Common Intervals

We can use common intervals as a measure of similarity between species. Disadvantage: All these measures do not reflect rearrangement operations or explain what happened to the genome over time. Rearrangement operations (events)

Back to our original problem: Given a set of genomes and a set of possible evolutionary events (operations), find a shortest set of events transforming those genomes into one another. What are the Rearrangement events (Operation)? These events (Operation) could be applied to a single gene or to a group of genes, intervals. Rearrangement

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