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Genetics
Outline
• Structure of DNA• DNA Functions• Transcription• Translation• Mutation• Cytogenetics• Mendelian Genetics• Quantitative Traits• Linkage
Structure of DNA
• DNA molecule organized into chain of nucleotides composed of three parts: Nitrogenous Base 5-Carbon Sugar (Deoxyribose) Phosphate Group
• Nucleotides bonded to each other forming a helix. Hydrogen bonds hold each base on one
side of the helix to another base on the other side.
Structure of DNA
• Four types of DNA nucleotides: Purines
- Adenine (A) and Guanine (G) Pyrimidines
- Cytosine (C) and Thymine (T)
Fig. 13.3
DNA Functions
• Storage of Genetic Information Gene - Segment of DNA that directs
protein synthesis.- Protein may act as an enzyme
influencing cell activities. Genome - Sum total of DNA in an
organism’s chromosomes.- Codon - Groups of three nucleotides.
DNA Functions
• Replication of Information Occurs during S phase of cell cycle.
- Strands of double helix unzip allowing DNA polymerase to pair individual nucleotides with the template strands.
Semi-Conservative Replication Occurs simultaneously in both
directions, and begins at several points simultaneously.
DNA Replication
DNA Functions
• Expression of Information Different subset of the genetic information
is read in each cell type. Expression requires two processes.
- Transcription - Copy of gene message using RNA.
- Translation Messenger RNA (mRNA) translated
to produce proteins.
Transcription
• Three different types of RNA produced: Messenger RNA (mRNA) Transfer RNA (tRNA) Ribosomal RNA (rRNA)
• RNA Synthesis Nucleotides added to single stranded DNA
molecule by RNA polymerase.- Only portions of the genome replicated.
Remainder is noncoding DNA.
Transcription
• Promoter Region signals transcription enzymes to attach to the DNA. Single-stranded RNA transcript produced.
• Chromosomes contain genes for building tRNA. Each form of tRNA has a specific
anticodon loop.- Base pairs with codon.
• Genes for rRNA also transcribed in the nucleus - Used to construct ribosomes.
Translation
• mRNA transcripts are decoded into proteins. Genetic code based on codons.
- 64 possible combinations Code for 20 amino acids.
• tRNA acts as a translator. Anticodon binds to mRNA codon.
• Start of translation signaled by a ribosome in the cytoplasm binding to the mRNA.
Fig. 13.5
Fig. 13.6
Fig. 13.6a
Fig. 13.6b-1
Fig. 13.6b-2
Fig. 13.6b-3
Fig. 13.6b-4
Fig. 13.6c
Central Dogma of Molecular Dogma
Mutation
• Mutations - Changes in DNA sequence. Mutagens - Agents that alter DNA.
- Ultraviolet light- Ionizing radiation
Somatic Mutation - Occurs in body cell. Germ-Line Mutation - Occurs in tissues that
will produce sex cells.- Passed on to future generations.
• All genetic variability due to mutations.
Cytogenetics
• Cytogenetics is the study of chromosome behavior and structure, from a genetic point of view.
• Changes in Chromosome Structure Inversion - Chromosomal piece is broken
and reinserted in the opposite orientation. Translocation - Chromosomal piece breaks
off and attaches to another chromosome.- Important in speciation.
Cytogenetics
• Changes in Chromosome Number Mistakes during chromosome pairing and
separation can result in gametes carrying extra or missing chromosomes.
- Aneuploid - Carries, or is missing, an extra chromosome(s).
- Polyploid - Has at least one complete extra set of chromosomes.
Mendelian Genetics
• Mendel crossed self-pollinated pea plants and recorded his observations. Crossed a tall plant with a short plant. Parental Generation (P)
- All offspring were tall.- First Filial Generation (F1)
Cross offspring yielded a ratio of three tall individuals to one short individual.
Second Filial Generation (F2)
Mendelian Genetics
• Law of Unit Characters Factors (Alleles), which always occur in
pairs, control the inheritance of various characteristics.
- Genes are always at the same position (locus) on homologous chromosomes.
• Law of Dominance For any given pair of alleles, one
(dominant) may mask the expression of the other (recessive).
Mendelian Genetics
• Phenotype refers to an organism’s physical appearance.
• Genotype refers to the genetic information responsible for contributing to the phenotype. Homozygous - Both alleles identical. Heterozygous - Alleles are contrasting.
Monohybrid Cross
• A cross is made between two true-breeding parents differing for a single trait, producing an F1 generation. These plants are intercrossed to produce
an F2 generation.
Fig. 13.10
Fig. 13.11
Dihybrid Cross
• Cross made for parents differing in two traits. Mendel’s Law of Independent Assortment
- Factors (genes) controlling two or more traits segregate independently of each other.
Linked genes do not segregate independently.
- Punnett Square used to determine genotypes of the zygotes.
Fig. 13.12
Mendelian Genetics
• Backcross A cross between a hybrid and one of its
parents.- Can be used to test inheritance theory.
• Incomplete Dominance Some genes exhibit traits in which a
heterozygote is intermediate in phenotype to the two homozygotes.
Fig. 13.13
Quantitative Traits
• Quantitative traits exhibit a range of phenotypes rather than discrete phenotypes studied by Mendel. Molecular geneticists are able to identify
chromosomal fragments, quantitative trait loci, associated with quantitative traits.
Extranuclear DNA
• Entranuclear DNA is found in both mitochondria and chloroplasts. Endosymbiont Hypothesis theorized
mitochondria and chloroplasts were free-living, and at some point became incorporated into cells of organisms that evolved into plants and established a symbiotic relationship.
Linkage and Mapping
• Genes that are together on a chromosome are said to be linked. Each gene has a specific location (locus)
on a chromosome.- Crossing-over should be more likely
between two genes located far apart on a chromosome that between two genes located closer together.
Crossing over frequency can be used to construct a genetic map.
1 map unit = 1% crossing over.
Hardy-Weinberg Law
• Hardy-Weinberg states the proportions of dominant alleles to recessive alleles in a normally interbreeding population will remain the same from generation to generation in the absence of external forces.
Review
• Structure of DNA• DNA Functions• Transcription• Translation• Mutation• Cytogenetics• Mendelian Genetics• Quantitative Traits• Linkage
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