genetically engineered plants

8/8/2019 Genetically Engineered Plants

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WATER DEFICIT is acritical environmentalfactors that limitagricultural production

worldwide. WATER DEFICIT

causes a series of morphological,physiological,

biochemical, andmolecular changes inplants.



Water deficit resistance-

Plants



Water deficit tolerance

in

transgenic

Arabidopsis thaliana



SNF1-related protein kinase 2 (SnRK2), SRK2C,

is an osmotic-stress-activated protein kinase in

Arabidopsis thaliana that can significantly impact

water deficit tolerance of Arabidopsis plants.

Knockout mutants of SRK2C exhibited water

deficit hypersensitivity in their roots, suggestingthat SRK2C is a positive regulator of water

deficit tolerance in Arabidopsis roots.



Materials and Methods

1. Generation of TransgenicPlants or Cells.

The coding region of SRK2CcDNA was amplified by RT-PCR

by using primers ( X

baIsite) and(BamHI site) and cloned into

pBE2113 vector using CaMV35S promoter.

Arabidopsis thaliana culturedcells were transformed by using Agrobacterium tume-faciens.

2.Cell Culture, Plant Growth,and Stress Treatments.



Cont,

3. Gel Kinase Assay.

4.Immunocomplex Kinase Assay

5.RNA Gel Blot Analysis.

6.Microarray Analysis and Data Mining.



RESULTS

Transgenic plants displayed higher overall water deficit tolerance than control plants.

Microarray analysis revealed that their water deficit tolerancecoincided with up-regulation of many stress-

responsive genes, for example, RD29A, COR15A, and

DREB1A/CBF3.

Results,concluded that SRK2C is capable of mediating signals

initiated during water deficit stress, resulting in appropriate

gene expression.



Transformation of tomato cultivar

`Pusa Ruby' with bspA gene from

Populus tremula for water deficit tolerance



bspA



Transgenic wheat

DREB1A



Result



Lily ASR Protein -Conferswater deficit tolerance in

Arabidopsis



LLA23



Result

LLA23 mediates stress-responsive ABA signaling.

In vegetative tissues, it is seen that transgenic plants

have a decreased rate of water loss and exhibitenhanced water deficit resistance.

LL A23-functions as water deficit resistant gene.



Effects of free proline accumulation

in petunias under water deficit



The transgenic plants accumulated Proline and their

drought tolerance was tested. The Proline content

amounted to 0.57±1.01% of the total amino acids in the

transgenic plants, under normal conditions.

The transgenic plant lines tolerated 14 days of drought

stress, which confirms that transgene had full

functionality.



Conclusions

W ater deficit-tolerance is acomplex phenomenon.

W hether transgenic plants will

ultimately contribute to the

struggle against world hunger,is a

Most test results are based on

greenhouse experiments.

There are no commercially

available varieties.



REFERENCES:

1-SRK2C, a SNF1-related protein kinase 2,

improves drought tolerance by controlling stress-

responsive gene expression in Arabidopsis

thalianaTaishi Umezawa , Riichiro Yoshida , Kyonoshin

Maruyama , Kazuko Yamaguchi-Shinozaki , and

Kazuo Shinozaki

PNAS | December 7, 2004 | vol. 101 | no. 49 |

17306-17311



2- Effects of free proline accumulation in petunias under water deficit stress

Mika Yamada, Hiromasa Morishita, Kaoru Urano, NorikoShiozaki, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozakiand Yoshu Yoshiba.

Journal of Experimental Botany 2005 56(417):1975-1981;

3- A Lily ASR Protein Involves Abscisic Acid Signaling and Confers Drought and Salt Resistance in Arabidopsis . .

Chin-Ying Yang, Yu-Chuan Chen, Guang Yuh Jauh andCo-Shine Wang.

Plant Physiology 139:836-846 (2005)



4-Effects of free proline accumulation in

petunias under w

ater deficit.

Mika Yamada, Hiromasa Morishita, Kaoru Urano, NorikoShiozaki, Kazuko Yamaguchi-Shinozaki, Kazuo

Shinozaki and Yoshu Yoshiba,

Central Research Laboratory, c/o Advanced ResearchLaboratory, Hitachi Ltd., 2520 Akanuma, Hatoyama-cho,Hiki-gun, Saitama 350-0395, Japan

Research Resources Center, Brain Science Institute,RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

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