Generating Spiral Waves with Different GCaL and GK1

Clara N. Jasmine M.

Rachel S.Mark D.

CMACS Workshop 2013

What do you see?

Effects on Action Potential ICaL is a depolarizing inward

current

Affects plateau of action potential, a balancing act between depolarizing currents

Hypothesis: An increase in GCaL, increases ICaL, which will elongate the plateau

Saeneno JB, Vrints CJ. Molecular aspects of the congenital and acquired Long QT Syndrome: clinical implications. J Mol Cell Cardiol. Epub 2008 Feb 9. http://www.ncbi.nlm.nih.gov/pubmed/18336833

Effects on Action Potential IK1, inward rectifying current,

closes at depolarization

Active during resting potential and the end of Phase 3 repolarization

Hypothesis: An increase in GK1, increases IK1, which will make the repolarization slope steeper

Saeneno JB, Vrints CJ. Molecular aspects of the congenital and acquired Long QT Syndrome: clinical implications. J Mol Cell Cardiol. Epub 2008 Feb 9. http://www.ncbi.nlm.nih.gov/pubmed/18336833

Effects on Spiral Waves Is there more

orange in the upper right quadrant?

Is there more green as Gk1 increases?

How can we improve the testability of our hypotheses?

Effects on Spiral Waves Is there more

orange in the upper right quadrant?

Is there more green as Gk1 increases?

How can we improve the testability of our hypotheses?

Increased GK1

Inward Rectifier K+ conductance

Higher GK1 Leads to Dispersion

Increased GK1

Inward Rectifier K+ conductance

Higher GK1 Leads to Dispersion

Gcal 25,Gk1 25

Increased GK1

Inward Rectifier K+ conductance

Higher GK1 Leads to Dispersion

Gcal 25,Gk1 25 Gcal 25,Gk1 145

Increased GK1

Higher value of GCaL

Increased GK1

Higher value of GCaL

Gcal 145,Gk1 25

Increased GK1

Higher value of GCaL

Gcal 145,Gk1 25 Gcal 145,Gk1 145

Increased GCaL

L-Type Ca+2 conductance

GCaL changes pathway shape

Increased GCaL

L-Type Ca+2 conductance

GCaL changes pathway shape

Gcal 25,Gk1 45

Increased GCaL

L-Type Ca+2 conductance

GCaL changes pathway shape

Gcal 25,Gk1 45 Gcal 125,Gk1 45

Increased GCaL

Higher value of GK1

Increased GCaL

Higher value of GK1

Gcal 25,Gk1 185

Increased GCaL

Higher value of GK1

Gcal 25,Gk1 185 Gcal 185,Gk1 185

ConclusionIncreased GK1 Leads to a Tip Trajectory

Spread Out Over a Larger Area.

Increased GCal Plays a Role in the Shape of the Pathway Traced By the Tip.

Acknowledgements!!!!!!1!!CMACS WorkshopProfessor GriffethProfessor FentonNSF/NIH/NASA/ABC/CIA/CUNY/LOLPeter’s PythonTNNP Mother Nature For Developing A

Complicated Heart Structure That Has Not Been Fully Understood Thus Far.

Questions?