general biology i fall 2003 – tentative lecture/laboratory

18
1 General Biology I Fall 2020 Lecture/Laboratory DRAFT Syllabus (8/5/2020) Marcy Kelly, PhD Phone: (212)-346-1353 Email: [email protected] Getting to Know Each Other: This is a REALLY large class AND the lecture is fully online. These present significant barriers with respect to getting to know each other. It is important to get to know each of you to support your success. It is important for you to get to know me too. I do not want to be just someone who teaches your Biology course but, rather a person who is on your side and here to support your learning. The best way for me to start our conversations is to invite you to attend my office hours. You can ask me questions about biology or chat with me about anything!!! Office Hours on Zoom by Appointment (e-mail me and I will send you a Zoom link): Tuesdays from 1:30- 4:00, Wednesdays from 10:30-12 and Fridays from 1-2. Communication between Class Sessions: The primary means by which I will communicate course announcements and information to you is through the Pace e-mail system. Please be sure to check your Pace e-mail AT LEAST once per day. Sometimes, for whatever reason, students who forward their Pace e-mails to their personal accounts do not receive e-mails that are sent from a course instructor through Blackboard. Please be sure that you can receive e-mails sent through Blackboard. Course Modality: The lecture portion of this course will be offered online using a synchronous format (you will join me on Zoom from 10:35-12 on T and R). I will present course materials, I will track your understanding of the materials during lecture using iClicker Cloud, and there will be Zoom breakout groups for in-class activities. There will also be three embedded tutors joining us at each lecture to assist by providing real-time learning support. The embedded tutors are undergraduate STEM majors who each took BIO 101 in the past with me and earned grades of A or B in the course. The laboratory portion of this course will be offered using a web-assisted format. The experiment you will perform in the laboratory is a semester-long research experiment (as opposed to one-off, individual weekly experiments). There are 20 students enrolled in each laboratory section. Students in the labs will be broken into five groups of four and one group member from each group will attend lab in person for three weeks of the laboratory course while the other three group members will Zoom in. After the first three weeks, the next group member will attend lab in person to continue the experimental work while the other three group members Zoom in. Then the next group member will go into the lab for three weeks and so on until all four group members have attended lab in person for three weeks each. Your laboratory instructors will work with you to organize this. Please do note the dates in the syllabus table, below, for the laboratory as they provide more information about the schedule. Some students will be placed into a required, online, weekly discussion group program on Zoom that will be facilitated by our embedded lecture tutors. Placement into this program will be determined by the scores

Upload: others

Post on 26-Jan-2022

0 views

Category:

Documents


0 download

TRANSCRIPT

1

General Biology I Fall 2020

Lecture/Laboratory

DRAFT Syllabus (8/5/2020)

Marcy Kelly, PhD

Phone: (212)-346-1353

Email: [email protected]

Getting to Know Each Other: This is a REALLY large class AND the lecture is fully online. These present

significant barriers with respect to getting to know each other. It is important to get to know each of you to

support your success. It is important for you to get to know me too. I do not want to be just someone who

teaches your Biology course but, rather a person who is on your side and here to support your learning. The

best way for me to start our conversations is to invite you to attend my office hours. You can ask me

questions about biology or chat with me about anything!!!

Office Hours on Zoom by Appointment (e-mail me and I will send you a Zoom link): Tuesdays from 1:30-

4:00, Wednesdays from 10:30-12 and Fridays from 1-2.

Communication between Class Sessions: The primary means by which I will communicate course

announcements and information to you is through the Pace e-mail system. Please be sure to check your Pace

e-mail AT LEAST once per day. Sometimes, for whatever reason, students who forward their Pace e-mails to

their personal accounts do not receive e-mails that are sent from a course instructor through Blackboard.

Please be sure that you can receive e-mails sent through Blackboard.

Course Modality: The lecture portion of this course will be offered online using a synchronous format (you

will join me on Zoom from 10:35-12 on T and R). I will present course materials, I will track your

understanding of the materials during lecture using iClicker Cloud, and there will be Zoom breakout groups

for in-class activities. There will also be three embedded tutors joining us at each lecture to assist by

providing real-time learning support. The embedded tutors are undergraduate STEM majors who each took

BIO 101 in the past with me and earned grades of A or B in the course.

The laboratory portion of this course will be offered using a web-assisted format. The experiment you will

perform in the laboratory is a semester-long research experiment (as opposed to one-off, individual weekly

experiments). There are 20 students enrolled in each laboratory section. Students in the labs will be broken

into five groups of four and one group member from each group will attend lab in person for three weeks of

the laboratory course while the other three group members will Zoom in. After the first three weeks, the

next group member will attend lab in person to continue the experimental work while the other three group

members Zoom in. Then the next group member will go into the lab for three weeks and so on until all four

group members have attended lab in person for three weeks each. Your laboratory instructors will work

with you to organize this. Please do note the dates in the syllabus table, below, for the laboratory as they

provide more information about the schedule.

Some students will be placed into a required, online, weekly discussion group program on Zoom that will be

facilitated by our embedded lecture tutors. Placement into this program will be determined by the scores

2

students earn on the Discussion Group Placement Exam that will be administered on R 9/3 (refer to syllabus

table, below). The discussion group is meant to support student learning and success. Students who are

placed into the discussion group program may place out of the program upon earning a midterm exam grade

of 70% or above. Students who were not initially placed in the discussion group program who score below

70% on the midterm exam will be placed into the discussion group program after the mid-term exam.

Therefore, all students should keep their schedules flexible to devote one hour of time on either Friday

afternoons or after the BIO 101 lab sessions for the discussion group program.

BIO 101 Embedded Tutors/Discussion Group Peer Leaders:

Jesse Devlin: [email protected]

Kourtney Kelly: [email protected]

Brookelynne Verrette: [email protected]

Required Lecture Texts:

• Short Guide to Writing about Biology, A, 9th Edition, By Jan A. Pechenik

• Mastering Biology with Pearson e-Text -- Standalone Access Card -- for Campbell Biology, 12th

Edition, By Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece

• Reading Primary Literature: A Practical Guide to Evaluating Research Articles in Biology, By

Christopher M. Gillen

• These books come as a bundle with MasteringBiology. They must be purchased from the online

Campus Store as the bundle is custom-made.

Required Laboratory Texts:

• Small World Initiative: A Research Guide to Microbial and Chemical Diversity, 4th Edition, By Simon

Hernandez, Tiffany Tsang, Carol Bascom-Slack, and Jo Handelsman

• Small World Initiative: Research Protocols, 4th Edition, By Simon Hernandez, Tiffany Tsang, Carol

Bascom-Slack, and Jo Handelsman

Other Required Materials (the first three on the list below can be purchased at the Campus Store):

• iClicker Cloud Account (https://www.iclicker.com/) on your laptop or mobile device for

lecture

• calculator

• lab coat and goggles

• a laptop or tablet that you can bring with you to lab (with Microsoft Office loaded onto it;

Pace students can download office for free at: https://aspnetweb.pace.edu/adam/)

• A webcam that is attached to your computer/tablet for exams

• A strong, uninterrupted internet connection

All Required Course Materials can be Purchased Online from the Campus Store.

3

Course Outcomes:

Biology Program Outcome BIO 101 Assessment Tool(s)

Develop a foundational knowledge of the

principles of modern biology.

Questions on MasteringBiology quizzes, iClicker

questions, discussion group modules and

questions on exams.

Outline and defend the role of evolution in all

disciplines of biology.

Questions on MasteringBiology quizzes, iClicker

questions and questions on exams.

Read and critique primary literature research

papers.

Lecture paper assignments and laboratory

report.

Design experiments. MasteringBiology questions and laboratory.

Generate and interpret graphs displaying

experimental data.

Graphing MasteringBiology quizzes and

semester long graphing assignment.

Analyze data. Weekly lab notebook and final laboratory report.

Communicate results in both written and oral

formats.

Final laboratory report and presentations in the

laboratory.

Use the scientific method to develop

questions and experimental methods that

integrate all areas of biology.

NA; this is an outcome for more advanced

biology courses.

LECTURE SCHEDULE

General Notes about the Lecture:

• Use the bulleted lists in the table below as your “To Do” list for each set of dates.

• The “To Do” lists are organized to ensure that you will have read the chapter and taken the

MasteringBiology Assessments BEFORE we begin each topic in lecture.

Date Topic/Assignments Chapter(s)

T 8/25, R

8/27, T

9/1

Introduction

What is Biology?

In Class Activity T 9/1: Graphing

On Your Own:

• Create your iClicker account to begin using during the 8/27 lecture ()

• Read Chapters 1, 2 and, 3 by T 9/1

• Watch the videos and review the associated lessons: Intro to Biology,

Chemistry of Life, Water/Acids/Bases by T 9/1

1

4

• Register for MasteringBiology and take the Tutorial and Assessment 1 by

T 9/1 at 10:35 AM

• Install Respondus Lockdown Browser and Respondus Monitor onto the

computer you plan to use to take exams for this course by R 9/3 at 10:35

AM

R 9/3 Take Discussion Group Placement Exam using Respondus Lockdown

Browser/Monitor

• Exam will go live at 10:35 AM and will shut down at 12:00 PM.

Please begin the exam at 10:35 AM.

On Your Own:

• Watch the videos and review the associated lessons: Properties of

Carbon, Macromolecules by T 9/8

• Take MasteringBiology Assessment 2 by 10:35 AM on T 9/8

• Start to think about what you want to do for your graphing experiment

(decision due by 9/10)

T 9/8, R

9/10, T

9/15, R

9/17

Macromolecules

On Your Own:

• Select an experiment to work on for the semester for the graphing

assignment and submit topic through the google doc link that I will post

to BB by 10:35 on R 9/10

• Read Chapters 6 and 27, Section 27.1 by T 9/15 (Please refer to the notes

provided on BB to determine important materials/page numbers to

know)

• Watch the videos and review the associated lessons: o Structure of a Cell, Bacteria and Archaea - content in Prokaryotic

Structure Section, only

o Through the Virtual Cell o Membrane Structure and Function by T 9/15

• Take MasteringBiology Assessment 3 by 10:35 AM on T 9/15

• Read Chapter 7 using lecture notes and PPT presentations posted on BB

to guide what you need to know (you DO NOT need to know every last

detail from the chapters)

• Watch the videos and review the associated lessons: o Membranes and Transport o Gradients

• Take MasteringBiology Assessment 4 by 10:35 AM on T 9/22

4,5

T 9/22, R

9/24

Biological Membranes

In Class Activity T 9/22: Interpreting Graphs

7

5

On Your Own:

• Read Chapter 8 using lecture notes and PPT presentations posted on BB

to guide what you need to know (you DO NOT need to know every last

detail from the chapters)

• Watch the videos and review the associated lessons: Energy and Enzymes

• Take MasteringBiology Assessment 5 by 10:35 AM on T 9/29

• Work on your graphing assignment

T 9/29, R

10/1, T

10/6

Introduction to Metabolism

On Your Own:

• Study for midterm exam

• No MasteringBiology Assessment due on T 10/6

8

R 10/8 Midterm Lecture and Laboratory Exam

On Your Own (After the Exam):

• Read Chapter 9, Sections 9.1-9.3 using lecture notes and PPT

presentations posted on BB to guide what you need to know (you DO

NOT need to know every last detail from the chapters)

• Watch the videos and review the associated lessons: o Cellular Respiration o Energy Consumption

o Glycolysis: An Overview o Glycolysis: The Reactions o The Citric Acid Cycle: An Overview o The Citric Acid Cycle: The Reactions

• MasteringBiology Assessment 6 will cover the first part of Chapter 9

(redox, glycolysis and Krebs cycle). Take MasteringBiology Assessment 6

by 10:35 AM on T 10/13

• Work on your graphing assignment

Chapters 1-

8, 27

Lab

Experiment

Numbers 1-

7

T 10/13,

R 10/15,

T 10/20,

R 10/22,

T 10/27

Cellular Respiration

In Class Activity T 10/27: Creating linear regression graphs

On Your Own:

• Read Chapter 9, Sections 9.4-9.6 using lecture notes and PPT

presentations posted on BB to guide what you need to know (you DO

NOT need to know every last detail from the chapters)

• Watch the videos and review the associated lessons: o Cellular Respiration (Khan Academy) o Cellular Respiration (YouTube)

9

6

• MasteringBiology Assessment 7 will cover the second part of Chapter 9

(electron transport chain, fermentation, and anabolic pathways). Take

MasteringBiology Assessment 7 by 10:35 AM on T 10/20

• Read Chapter 10 using lecture notes and PPT presentations posted on BB

to guide what you need to know (you DO NOT need to know every last

detail from the chapters)

• Watch the videos and review the associated lessons: o Photosynthesis (Khan Academy) o Photosynthesis (YouTube) o Photosystem II

• Take MasteringBiology Assessment 8 by 10:35 AM on T 10/27

• Work on your graphing assignment

R 10/29,

T 11/3, R

11/5

Photosynthesis

On Your Own:

• Read Chapters 11 and 12 using lecture notes and PPT presentations

posted on BB to guide what you need to know (you DO NOT need to

know every last detail from the chapters)

• Watch the videos and review the associated lessons:

o Cell Signaling, Cell Division – content in Intro to Cell Division and

Cell Cycle and Mitosis, only

o Insulin Signaling

o Cell Communication

o Mitosis

• Take MasteringBiology Assessment 9 by 10:35 on T 11/3

• Read Chapter 13 using lecture notes and PPT presentations posted on BB

to guide what you need to know (you DO NOT need to know every last

detail from the chapters)

• Watch the videos and review the associated lessons:

o Cell Division – content in Meiosis, only

o Meiosis

o Meiosis and Sexual Life Cycles

• Take MasteringBiology Assessment 10 by 10:35 on T 11/10

• Work on your graphing assignment

10

T 11/10,

R 11/12,

T 11/17

Meiosis

On Your Own:

13

7

• Read Chapter 16 using lecture notes and PPT presentations posted on BB

to guide what you need to know (you DO NOT need to know every last

detail from the chapters)

• Watch the videos and review the associated lessons: o DNA as the Genetic Material o The Molecular Basis of Inheritance

• Take MasteringBiology Assessment 11 by 10:35 AM on T 11/17

• Work on your graphing assignment (due T 11/24)

R 11/19,

T 11/24

The Molecular Basis for Inheritance

In Class Activity R 11/19: Meselson-Stahl Experiments

On your Own:

• Submit graph assignment by T 11/24 by 11:59 PM

• Read the Evolution at the Cellular Level document and notes posted to BB

and Chapter 25, Sections 25.1 and 25.3

• Watch the videos and review the associated lessons: History of Life on

Earth

• Take MasteringBiology Assessment 12 by 10:35 AM on T 11/24

16

R 12/3 FINAL CUMMULATIVE LECTURE AND LABORATORY EXAMINATION 1-13, 16, 27

Experiment

Numbers 1-

9

NYC LABORATORY SCHEDULE

General Notes about the Laboratory:

• This laboratory experience will follow the curriculum created at Yale University through the Small World

Initiative (SWI).

• To prepare for lab each week, you should read the relevant pages in the Research Guide and Research

Protocol Book and then fill in the Background, Objective, Hypothesis/Rationale, and Protocol fields for

each experiment in the Research Guide. Your work will be checked in the laboratory each week.

• There are two SWI YouTube Channels with videos of techniques for each lab exercise to help you prepare

for lab: SWI 1 and SWI 2.

• Prior to the start of each lab session, you will have a quiz created by and administered by your laboratory

instructor to confirm that you are prepared for the laboratory session. The quiz will be based upon the

materials presented in the Research Guide and Protocol Book.

• As described on page 1 of this syllabus, each lab section will be broken into five groups of four. Every

three weeks, a different group member from each group will come to lab to complete the exercise while

8

the other members Zoom in. More information is provided in the syllabus, below. Your laboratory

instructor will contact you before your first laboratory session to organize this further.

• Additional homework outside of the weekly work described above is in the table below.

• Lab exam questions will appear on the lecture exams.

Week, Dates Experiment

Number

Pages in Resources Changes/Notes/Hints/Assignments

GROUP MEMBER 1 ATTENDS LAB IN PERSON FOR THE FIRST THREE SESSIONS, ALL OTHER GROUP MEMBERS ZOOM

IN TO LAB. GROUP MEMBER 1 WILL SERVE AS ZOOM HOST.

Week 1:

M 8/24, T

9/1-F 9/4

1, Using

Common

Laboratory

Tools to

Evaluate

Measurements

Handout

Research Guide: 2-15

Research Protocols:

None

Handout

• Plating beads will not be available

• You will each receive one sterile 50 ml conical to

collect dirt for next week’s experiment.

• Please note that it is illegal to obtain dirt from City of

NY parks or to bring soil into NYC from other states.

Homework

for Week 2:

M 8/31, T

9/8-F 9/11

2 Research Guide: 16-23

Research Protocols: 46-

47

• Complete pages 20-22 in the Research Guide (as well

as regular assignment for the week).

Week 2:

M 8/31, T

9/8-F 9/11

3 Research Guide: 24-33

Research Protocols: 50,

58-65

• Look at results from last week and record findings on

pages 14-15 of Research Guide.

• Please notice the Laboratory Data Collection

Summary Sheet on 33 of the Research Guide and the

Soil Sample Data Collection Sheet on page 40 of the

Research Guide. You might want to start filling in

these sheets so that you have your data all set for

the lab report at the end of the semester.

Week 3:

M 9/14- F

9/18

5 Research Guide: 34-37,

41-48

Research Protocols: 31-

32, 48-49

• We are not performing Experiment 4 but, you should

read the preamble for that experiment.

• Look at results from last week and record findings on

pages 30-32 of Research Guide and page 62 of

Research Protocols book. The table for CFU/gram

recording in the Research Protocol book is clearer

than the table on page 32 of the Research Guide –

your instructor will let you know whether you should

complete the table in the Protocol book over the

Research Guide.

9

• Record your “number of isolates picked” and

“characteristics of isolates” answers on page 47 of

Research Guide during class.

• We will not be using a UV light to help describe

colony characteristics as indicated in the colony

morphology protocol (page 32 of the Research

Protocols book).

• Each lab table should select an ESKAPE pathogen to

research for homework and work with for the next

lab session. There are five ESKAPE pathogens that

will be available so, each table should select one to

ensure that all are covered. We do not have

Acinetobacter baylyi so, do not select Aceinetobacter

baumannii for your research.

Homework

for Week 4:

M 9/21 -F

9/25

6 Research Guide: 49-56

Research Protocols:

None

• Use pages 55-56 in the Research Guide to guide your

ESKAPE pathogen research. Each group should

prepare a PPT describing their research for

presentation during lab session. PPT template is

located in both your lecture and laboratory

Blackboard shells. Your presentations should be no

longer than 10 minutes. Do not add extra slides to

the PPT template. Just fill it in.

GROUP MEMBER 2 ATTENDS LAB IN PERSON FOR THE NEXT THREE SESSIONS, ALL OTHER GROUP MEMBERS ZOOM

IN TO LAB. GROUP MEMBER 2 WILL SERVE AS ZOOM HOST.

Week 4:

M 9/21 -F

9/25

7, Using

Common

Laboratory

Tools to

Evaluate

Measurements

Handout

Research Guide: 57-71

Research Protocols: 53-

54

Handout

• Perform the “Using Common Laboratory Tools to

Evaluate Measurements” Exercise that the Group 1

students performed on the first day of lab to make

sure you know how to use some of the common

equipment.

• Look at results from last week and complete page 48

in the Research Guide.

• We will not be performing the patch/patch or top

agar screen. Student will perform only the

spread/patch screen.

Week 5:

M 9/28 – F

10/2

7 Research Guide: 57-71

Research Protocols: 53-

54

• Look at results from last week and identify antibiotic

producers. Begin to record identity of antibiotic

producers on page 69 of Research Guide and pages

57-58 of the Research Protocols book. Those

organisms will be used for this week’s experiment.

10

Monday 9/28 is Yom Kippur. Classes are scheduled online using an asynchronous format. We will need to have lab

that day to move the experiment forward but, your instructor will be flexible and will work with your class to

determine who can come in to do the work.

STUDY FOR LECTURE/LAB MIDTERM EXAM SCHEDULED ON R 10/8

Week 6:

M 10/5- F

10/9

7, Dilution

streak to

create single

colony stock

plates of

antibiotic

producing

isolates

Research Guide: 57-71

Research Protocols: 53-

54, 71-76

• Look at results from last week to identify isolates

that inhibit the growth of the most safe-ESKAPE

pathogens. Finish filling out pages 69-71 of Research

Guide and 57-58 of Research Protocol book with

your data.

• Each student group should select one isolate to

continue working with for the rest of the semester.

The best isolate to select is one that inhibits the

growth of the most safe-ESKAPE pathogens.

GROUP MEMBER 3 ATTENDS LAB IN PERSON FOR THE NEXT THREE SESSIONS, ALL OTHER GROUP MEMBERS ZOOM

IN TO LAB. GROUP MEMBER 3 WILL SERVE AS ZOOM HOST.

Week 7:

M 10/12- F

10/16

8, 9, Using

Common

Laboratory

Tools to

Evaluate

Measurements

Handout

Research Guide: 72-86

Research Protocols: 33-

34, 36, 17-18

• Perform the “Using Common Laboratory Tools to

Evaluate Measurements” Exercise that the Group 1

students performed on the first day of lab to make

sure you know how to use some of the common

equipment.

• We will not be performing the Gram stain.

• For Experiment 8, we will perform only the PCR this

week and will use the PCR bead method.

• The instructions for using the PCR machine are

below.

• For Experiment 9, we will only prepare the plates

this week.

Homework

for Week 8:

M 10/19 – F

10/23

Students will read Davis et al, 2016 and be prepared for a

group activity in lab on the paper. For credit, students

will need to bring a hard copy of the paper with

highlighting and annotations to demonstrate that they

read through the paper thoroughly. Students are not

expected to understand everything in the paper so, it is

expected that there will be a lot of questions in the

annotations.

Week 8: 8, 9 Research Guide: 72-86 • For Experiment 8, we will perform only the gel

electrophoresis this week. Results from gel

11

M 10/19 – F

10/23

Research Protocols: 13-

15, 19

electrophoresis should be recorded on page 78 of

the Research Guide.

• For Experiment 9, the student groups will chop up

the plate they struck out last week and put the

pieces in 50 ml conical vials (not glass bottles). We

will freeze the conical vials with the chopped up

plate pieces at -20oC for the week.

Week 9:

M 10/26 – F

10/30

8, 9, Handouts Research Guide: 72-86

Research Protocols:

Provided in lab and 19-20

• For Experiment 8, the student groups will perform a

Qiaquik PCR Purification on the PCR reactions that

yielded a 16S rRNA band on the gel. The instructions

for the PCR Purification kit will be in the lab and are

below.

• Student groups will also prepare purified PCR

samples to send out for sequencing. A protocol for

this will be present in the lab and appears below.

Instructions on how to use the nanodrop also

appears below.

• For Experiment 9, the student groups will perform

the first part of the organic extraction we will be

following the methanol extraction protocol but,

rather than resuspending the frozen, chopped up

plate pieces in methanol (page 20, Session 3A, step

2), the students will resuspend them in 15 mls of

75% methanol : 25% acetic acid. Student groups

should record relevant information from this

experiment on pages 85-86 in the Research Guide.

Homework

for Week 10:

M 11/2 – F

11/6

Handout and

peer review

rubric

• Please prepare a draft laboratory report. Although

you have not collected all of your data yet, you have

enough to draft a laboratory report. See the

handout and peer review rubric (Blackboard) for

guidance on what needs to be in the laboratory

report.

• The draft needs to be submitted to your laboratory

professor prior to the laboratory session. Your

laboratory professor will forward your report to 2 of

your peers for peer review at the beginning of the

laboratory session.

GROUP MEMBER 4 ATTENDS LAB IN PERSON FOR THE NEXT THREE SESSIONS, ALL OTHER GROUP MEMBERS ZOOM

IN TO LAB. GROUP MEMBER 4 WILL SERVE AS ZOOM HOST.

12

Week 10:

M 11/2 – F

11/6

9, Using

Common

Laboratory

Tools to

Evaluate

Measurements

Handout

Research Guide: 80-86

Research Protocols: 20-

21

• Perform the “Using Common Laboratory Tools to

Evaluate Measurements” Exercise that the Group 1

students performed on the first day of lab to make

sure you know how to use some of the common

equipment.

• Perform the second part of the methanol extraction

as indicated in the lab manual (no changes). Be sure

to pre-weigh the scintillation vials using the

analytical balance in the lab prior to transfer

(optional step 2 in protocol on page 21 of the

Research Protocol book).

Week 11:

M 11/9 – F

11/13

8, 9 Research Guide: 72-86

Research Protocols: 16

(first three paragraphs),

24-25, 22-23

• Enter sequencing findings onto pages 78-79 in

Research Guide.

• For Experiment 9, perform the antibiotic activity

assay on all safe-ESKAPE pathogens. Be sure to re-

weigh the scintillation vials to calculate the mass of

the dried extract (record the mass on page 86 of the

Research Guide under the “Data and observations”

heading.

• Resuspend dried extract in methanol to a

concentration of 10 ug/ul – if the volume

determined from the calculations is not large. If it is,

resuspend the dried extract in 160 ul of methanol

and adjust volume higher, if needed, to fully

resuspend.

Week 12:

M 11/16 – F

11/20

9 Research Guide: 80-86

Research Protocols: 23

• Evaluate for presence and diameter of zones of

inhibition and enter findings onto pages 85-86 of

Research Guide.

Week 13:

M 11/23 – T

11/24, W

12/2 – F

12/4

LAB REPORT DUE ON

DAY OF REGULARLY

SCHEDULED LAB

SUBMISSION METHOD TBD BY LAB INSTRUCTOR

Attendance:

According to the Department of Biological Sciences Student Handbook, “students are expected to attend ALL

lecture, laboratory and discussion group sessions.” Attendance will be taken during lecture, laboratory, and

during the discussion groups (if you are required to attend the discussion group). This course is extremely

fast-paced and, as you will see, attendance at lecture is essential in order to keep up with the material.

13

Because of the large lecture size, attendance will be monitored with in-class surveys using the Clickers system

(refer to Exams and Quizzes section below for more details). It is also critical that all students attend each

laboratory session (either in-person or by Zoom as per this syllabus) as no session can be made up. Thus,

each unexcused lab session absence will result in a reduction in the final laboratory grade at the laboratory

instructors’ discretion. Failure to attend a total of 3 laboratory sessions (excused or unexcused) will result in

a grade of 0 in the laboratory component of the course. As the discussion group component of the final

grade is based upon your participation in the groups, each unexcused absence from the discussion group will

result in a reduction of the discussion group final grade. Failure to attend a total of 3 discussion group

sessions (excused or unexcused) will result in a grade of 0 for the discussion group portion of the final BIO

101 grade. In addition, persistent lateness to lecture, laboratory, and/or discussion groups or leaving early

will also result in a reduction of grade at the instructor’s discretion.

A student may be excused from lecture, laboratory, and/or discussion group upon presentation of a medical

note from a physician, or evidence of a “catastrophic event,” (involving you or a family relative) indicating

that it was physically impossible to attend that session within two weeks of the absence. These notes will be

checked.

Assessments:

There will be a midterm and a cumulative final examination in lecture. The exams will have both lecture and

laboratory questions on them. There will be absolutely NO make-up exams unless there is an excusable,

documented absence. The documentation must be provided to me within 2 weeks of the missed exam and

will be checked.

MasteringBiology – Each student will be required to participate in weekly formative assessments using

MasteringBiology. The grade you earn on the assessments will not be counted in your final grade but, rather

your completion of the assessment will be considered (completion of assessment = 100%, incomplete or no

assessment – 0%). The MasteringBiology assessments will consist of guided questions to aid in the pre-

learning of the BIO 101 material (the number of questions will vary each week). The assessments will all

require reading passages, analyzing data, and problem solving that will help you prepare for topics discussed

in lecture and discussion group. You will notice that you can ask for hints to help you answer each question.

You are encouraged to ask for hints to help you navigate the questions. Please be aware that the

MasteringBiology assessments cannot be completed in a few minutes. You will need to block out at least one

continuous hour to an hour and a half for the assessments. The time you take to complete each assessment

will be monitored and, if your time is below 30 minutes, you will not receive credit for the assessment.

Please be sure that your internet connection is continuous. The assessments will log off if your internet

connection is interrupted and, you will be unable to complete the assessment. They will become available

for you to take them on Friday evenings after 5:00 PM and will go offline on Tuesday mornings at 10:35 AM.

Each student is required to practice using MasteringBiology by taking the tutorial offered through the

program. Students must complete the tutorial by Thursday 9/1/2020 at 10:35 am. The first

MasteringBiology assessment will be available for you to take beginning on Monday 8/24/2020 at 5:00 PM.

The dates/topics for each assessment is as follows:

14

Assessment Number Date Assessment Goes Live

(Fridays at 5:00 PM)

Due Date

(Tuesdays at 10:35 AM)

Chapters

Tutorial Monday 8/24, 9:00 AM 9/1 NA

1 Monday 8/24, 9:00 AM 9/1 1-3

2 9/4 9/8 4,5

3 9/11 9/15 6,27

4 9/18 9/22 7

5 9/25 9/29 8

6 10/9 10/13 9

7 10/16 10/20 9

8 10/23 10/27 10

9 10/30 11/3 11,12

10 11/6 11/10 13

11 11/13 11/17 16

12 11/20 11/24 Evol Bio

Clicker Questions - Beginning on Thursday 8/27/2020, clicker questions will be asked sporadically during

lectures to evaluate your understanding of the materials being presented. You will receive 1 point for every

correct answer.

Assignments:

All lecture and laboratory assignments must be typed. Any lecture or laboratory assignments that are

handwritten will not be graded. Discussion group assignments may be handwritten.

Lecture assignments: May be given sporadically throughout the course.

Laboratory assignments: Laboratory assignments and their due dates are listed in the syllabus (see above)

and no assignments will be accepted after their due dates. The laboratory is what makes this course a writing

enhanced course. Please be attentive to the feedback that your professors provide you on those

assignments. Additionally, you will have the opportunity to submit a draft of your laboratory report for

ungraded feedback. The drafts will then be revised based on the feedback received and resubmitted for a

grade.

Preparation for the Laboratory:

You are required to be thoroughly prepared for all experiments to be performed in each of the laboratory

sessions. Some of the sessions in this course are very time consuming and do not permit extensive discussion

of the exercises before performing them.

15

Studying for this course:

You should spend a minimum of three hours out of class for every one hour in class. This means nine hours

minimum a week outside of class studying for lecture. This does not include the amount of time required for

preparing for the laboratory portion of this course. You will be spending a SUBSTANTIAL amount of your

time on this course!!!!

Grading:

The lecture grade is worth 50% of your final grade and the laboratory grade is worth 50% of your final grade.

If you are enrolled in the discussion group, that grade will be part of the participation grade. You must pass

both the lecture and laboratory components of the course with a minimum grade of D (60%) in order to pass

the course. This means that you must earn 27/45% in lecture and 27/45% in laboratory. If you earn scores

below these in EITHER the lecture or laboratory, you will earn an F in the course. Please also note that you

must earn a final grade of C- or better in BIO 101 to enroll in BIO 102. Students may only enroll in BIO 101

twice as per the Biology Department Student Handbook.

Mastering Biology Assessments 6% (0.5 points each)

Clicker Questions 1%

Final Graphing Assignment 5%

Participation1 8%

Midterm Exam 15%

Cumulative Final Exam 15%

TOTAL 50 %

ESKAPE Pathogen Presentation 2.5%

Laboratory Report 20%

Primary scientific literature assignment (Davis et al, 2016): 2.5%

Weekly Laboratory Notebook Checks (Weeks 2-11) 5% (0.5 points each)

Laboratory Quizzes (Weeks 2-11) 20% (1 point each)

TOTAL2 50 %

1 Participation in lecture will be evaluated by your full and dedicated involvement in and full completion of

the active learning assignments in lecture. There are four pre-planned In Class Activities (refer to syllabus

table above) and there will be several In Class Activities that will be developed on the fly. For students who

are not enrolled in the discussion group, the participation grade will be determined by giving each activity

16

equal weight (for example, if there are eight activities, each one will be worth 1% of the participation grade).

If you are enrolled in the discussion group, 4% of the participation grade will be for attendance and full

participation during the discussion groups. The rest of the participation grade will be determined by giving

each activity equal weight (for example, if there are eight activities, each one will be worth 0.5% of the

participation grade). Note: Consistent attendance at my office hours with well thought out questions will

positively impact your participation grade, as well.

2 Try as we may, because there are so many different laboratory instructors involved in teaching and

evaluating student performance in this course, it is sometimes difficult to maintain grading consistency

between the different laboratory sections. Over the past 17 years, the average BIO 101 laboratory grade is

typically between 70-75%. To ensure fair evaluation of students across all sections, if needed, laboratory

final grades will be adjusted at the end of the semester to meet this average range.

Grade Scale:

A 100%-94% B- 83%-80% D+ 69%-67%

A- 93%-90% C+ 79%-77% D 66%-60%

B+ 89%-87% C 76%-74% F below 60%

B 86%-84% C- 73%-70%

Extra Credit:

According to the Department of Biology Student Handbook, “individual students will not be permitted to do

extra credit work, i.e., work beyond specified course requirements, unless the entire class has been given the

option to do so.” Extra credit MAY be given periodically throughout the course.

Academic Integrity:

Students in this course are required to adhere to Pace University's Academic Integrity Code. The Academic

Integrity Code supports honesty and ethical conduct in the educational process. It educates students about

what constitutes academic misconduct, helps to deter cheating and plagiarism, and provides a procedure for

handling cases of academic misconduct. Students are expected to be familiar with the Code, which can be

found under “University Policies” in the Student Handbook.

Individual schools and programs may have additional standards of academic integrity. Students

are responsible for familiarizing themselves with the policies of the schools, programs, and courses in which

they are enrolled.

Accommodations for Students with Disabilities:

The University’s commitment to equal educational opportunities for students with disabilities includes

providing reasonable accommodations for the needs of students with disabilities. To request an

accommodation for a qualifying disability, a student must self-identify and register with the Coordinator of

17

Disability Services for his or her campus. No one, including faculty, is authorized to evaluate the need and

arrange for an accommodation except the Coordinator of Disability Services. Moreover, no one, including

faculty, is authorized to contact the Coordinator of Disability Services on behalf of a student. For further

information, please see Information for Students with Disabilities on the University’s web site.

Pace University COVID-19 Safety:

CDC guidelines and University policy require proper face covering use in all classrooms, conference rooms,

hallways, elevators, and other common areas. Face coverings are required even if you are more than 6 feet

from another individual in a common indoor area. We must work together to protect others and ourselves

from the transmission of COVID-19. Any student entering class without a face covering will be asked to: a)

don a face covering or b) go to Security to obtain a face covering if the student does not have one. Students

who do neither of these things will be asked to leave class and they will be marked absent for the session.

Students are expected to be familiar with the current COVID-19 regulations, which are posted on the Return

to Campus website. See also up-to-date policies and announcements here and more information about Pace

University’s response to COVID-19.

To register for BIO 101 Fall 2020:

1. Go to https://www.pearson.com/mastering.

2. Under Register, select Student.

3. Confirm you have the information needed, then select OK! Register now.

4. Enter your instructor’s course ID: kelly44288 and Continue.

5. Enter your existing Pearson account username and password to Sign In.

You have an account if you have ever used a MyLab or Mastering product.

» If you don’t have an account, select Create and complete the required fields.

6. Select an access option.

» Enter the access code that came with your textbook or that you purchased

separately from the bookstore.

» If available for your course,

• Buy access using a credit card or PayPal.

• Get temporary access.

If you're taking another semester of a course, you skip this step.

7. From the You're Done! page, select Go To My Courses.

8. On the My Courses page, select the course name BIO 101 Fall 2020 to start your work.

18

To sign in later:

1. Go to https://www.pearson.com/mastering.

2. Select Sign In.

3. Enter your Pearson account username and password, and Sign In.

4. Select the course name BIO 101 Fall 2020 to start your work.

To upgrade temporary access to full access:

1. Go to https://www.pearson.com/mastering.

2. Select Sign In.

3. Enter your Pearson account username and password, and Sign In.

4. Select Upgrade access for BIO 101 Fall 2020.

5. Enter an access code or buy access with a credit card or PayPal.

Create an iClicker Reef Account:

To sign up for an iClicker Reef Account, you can either download the iClicker Reef mobile app from the App

Store or Google Play, or by visiting the Reef web application.

Go to CREATE AN ICLICKER REEF ACCOUNT to view a video on how to set up your account.

The course name is: Pace University BIO 101 Fall 2020

Please register with you University ID. The University ID starts with a “U” and then includes 8 digits. It is

NOT your e-mail address.