gene disruption (cont) & protein-protein interactions october 4, 2006 outline recap gene...
TRANSCRIPT
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Gene Disruption (cont) & Protein-Protein Interactions
October 4, 2006
Outline• Recap
• Gene Disruption and Drug Discovery
• RNAi
• Two Hybrid
• Affinity Purification
• Protein Chips
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Last Time1) Insertional Mutagenesis
– Transposon Strategies • Use reporter constructs to make enhancer traps and
gene fusions
Gene Traps: Gene Fusion
lacZ--Missing Promotor & ATG
Gene Fusions
2) Systematic KnockoutsBar coded
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Yeast Strains With Tagged Knockouts
ORFs onchromosome
TaggedPCR
product
Taggedyeast
strains
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Functional Analysis of Knockout Strains
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Using Deletions to Profile Drug Sensitivity
Giaever et al. Nature Genetics 1999 vol 21, 278-283
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Using Deletions to Profile Drug Sensitivity
ALG7YMR007
Giaever et al. Nature Genetics 1999 vol 21, 278-283
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Using Heterozygous Deletions to Profile Drug Sensitivity
(3503 strains 78 Compounds)
Lum et al. Cell 2004 Vol 116
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Using Heterozygous Deletions to Profile Drug Sensitivity
(3503 strains)
Lum et al. Cell 2004 Vol 116
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C. elegans RNAi = RNA interference
dsRNA inhibits gene expression
AAAAA
+AA
AA
by degrading its complementary mRNA
dsRNA
mRNA
Fire and Mello 1998 Nature
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Genome Wide Approach
Clone genes into E. coli Expression vector that makes dsRNA
ORF
Feed Worm E. coli;Score phenotype
E. coli
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RNAi
16,757 (86%) C. elegans Genes RNAied; 1,722 Mutant phenotypes Ahringer et al., Kohara et al.
Can be used for many organismsDrosophila, Mammalian Cells
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Mammalian RNAi Retrovirus Vector
From RNAi consortium
Virus
PackagingCells
Infect Cells
shRNAExpression
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Identification of Tumor Suppressors Using RNAiKlofcshoten et al. (2005) Cell 121, 849-858
1° Fibroblasts from humans die
Tr(-onc) Engineered Fibroblasts(hTERT, small t Antigen, p53-, p16-)“almost transformed”
Tr(-onc) Engineered Fibroblasts + RASV12
Transformed and form colonies
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Identification of Tumor Suppressors Using RNAi
Klofschoten et al. (2005) Cell 121, 849-858
Tr(-onc) cells
shRNA library (against 4000 genes)
New Tumor Suppressor:PITX1
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RNAi
• Simple and Inexpensive
• Systematic method--Comprehensive
• Knockout expression of gene families
Advantages
Disadvantages• Some Genes Not Affected
• Limited alleles
• Off target effects
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Uses of Knockouts: Summary
• Score phenotype to understand gene function
• Group different genes together based on phenotype
• Find new interesting genes
• Drug discovery
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Global Protein::Protein Interactions
• Three Methods:
1) Two Hybrid
2) Complex Analysis: Affinity
tagging/Mass Spectroscopy
3) Protein Chip
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Two-Hybrid System For DetectingProtein-Protein Interaction
Protein AGal4 DNABinding Domain
Protein BGal4Activation Domain
Genes
FusionProteins
HIS3Gal4 DNABinding Sites Selectable Marker
HIS3 Expression
If A & B interact
Colonies Grow On Plates Lacking
Histidine
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Cloning Strategy
ATG TAA
Primer 1 Primer 2Yeast ORFs
GAL
PCR
DBGAL
DB:: ORF
coTransform+ Vector
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Screening 6200 X 6200 Interactions
MATa strainsContaining
GalAD construct
Mate to MAT strain Containing DB construct
MATa/MATa Diploids
Assay Expressione.g His+ colonies
HIS3
DB::Protein AFusion
AD::Protein B Fusion
Black = Colonies
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Large Scale Two-Hybrid Screening
Sequence Adjacent to ORF for each plasmid (Interaction Sequence Tags ISTs)
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Results of Two Studies
1) 4,549 Interactions Among 3,278 Proteins (Ito et al.)
2) 957 Interactions 1004 proteins (Utez et al.)
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Interaction Map of Spindle Pole Body (MTOC) Proteins
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Interaction Map of Vesicular Transport Proteins
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A Comprehensive Protein Interaction Map
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Overlap of the Two Hybrid Studies
541 135 691
69 9
257
Utez et alPool Approach
Ito et alPool Approach
Utez et al. Individual Screen
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8,100 X 8,100 ORFs (~7,200 genes)
(1 DB clone X pool of 188 AD clones)
Human Two Hybrid Screen
10,597 Interactions2,754 Nonredundant
Rual et al. Nature 2005
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Human Two Hybrid Map
Rual et al. Nature 2005
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Human Two Hybrid Map
Disease Genes
(121 genes (green))
Rual et al. Nature 2005 Vol 437
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Two Hybrid
Advantages- In vivo Assay- Fairly Simple
Disadvantages- Hard to execute on a large scale- Prone to artifacts 50% False +s- Interactions mediated in nucleus
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Tandem Affinity Purification (TAP) Tagging
ORF Cam-Binding Domain
TEVProtein A
(IgG Binding)
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TAP ApproachIgG Beads
YFP
Contaminants
AssociatedProteins
YFP
Protein A Purification
TEV Protease,CBD Purification Cam
Beads
YFPYFPElute EGTA
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Identify Proteins by Mass Spec
Excise Band;Digest with Trypsin;Run Mass Spec
Load on SDS Gel
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TAP Purification of The U1 Splicing Complex (Snu71p)
(New)
(New)
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Many Complexes Are Conserved
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Affinity Purification/Mass Spec Analysis of Complexes - Yeast
4,562 Purifications (Krogan et al. 2002)2,357 Successful
4,087 Interacting Proteins7,123 Core Interactions (2,708 proteins)14,317 Extended (3,672 proteins)
547 Complexes
Krogan et al. Nature 2006 Vol 440
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Two HybridSize and Conservation of the Complexes
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TAP Tag Approach
Advantages- In Vivo Assay- Identifies Entire Complex
Disadvantages- Interactions may be indirect- Likely to miss some rare components- Contaminants may copurify
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Summary• Affinity Purification: ~10,000 High Confidence
Interactions Among ~2000 Proteins
• Two Hybrid: >4,549 Interactions Among 3,278 Proteins
• >20,000 Interactions
• Combining Data = More Accuracy
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What is a Protein Microarray?
A high density array containing 100s to many thousands of proteins
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DNA Microarrays
• Analyzing Gene Expression
• Mapping Mutations
• Mapping Transcription Factor Binding Sites
Awesome
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• Protein Function
• Protein Modification and Regulation
• Protein Pathways
• Protein Profiling
• Drug Discovery and Development
Protein Microarrays
Awesome2
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Antigens Protein-Protein Interactions
Small Molecule Interactions
ADPATP
EnzymaticAssays
Antibody Microarrays Functional Protein Microarrays
Two Types of Protein Microarrays
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Microarray assay of a human serum sample. A 15 µL sample of human serum was incubated for 30 min on a microarray with 75 different anticytokine antibodies printed in quadruplicate. Following washing and incubation with a mixture of secondary antibodies to each cytokine, detection was carried out using RCA. The fluorescent image was obtained using GenePix software on an Axon Microarray Scanner. The enlarged image shown represents one-eighth of the data acquired from a 1' × 3' microscope slide. Fluorescent intensities are represented in pseudocolor, with lowest intensities in blue and highest intensities in white.
Cytokine Detection withAntibody Microarray
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Human Allergen Microarray