Scientific Organizers: Matthias Hebrok, University of California, San Francisco, USASeung K. Kim, Stanford University, USA Felicia Pagliuca, Semma Therapeutics, Inc., USA Anil Bhushan, University of California, San Francisco, USA

Diabetes mellitus is a pandemic disease rooted in defects of multiple organs. Autoimmune destruction of pancreatic islet beta cells (type 1 diabetes) or relentless deterioration in beta cell function from excessive systemic insulin demand (type 2 diabetes) culminates in severe or lethal metabolic derangement. Inter-organ signaling has emerged as a crucial mechanism for achieving metabolic balance, including responses by peripheral insulin-target organs that, in turn, remotely regulate islet activity. This meeting will focus on crucial aspects of beta cell biology, including islet development, pancreas cellular plasticity, beta cell surrogate generation from stem cells, novel gene editing technologies, and immune-islet interactions, as well as translation of basic discoveries into novel therapeutic trials for diabetic patients. This meeting will encourage the presentation of novel concepts, foster useful interactions between participants from academia and industry, and accelerate interdisciplinary approaches in diabetes research.

Session Topics:• Beta Cell Development/Maturation • Human Islet Biology• Alpha Cells/Intracellular Islet Communication • Emerging Technologies and Disease Modeling• Progenitor to Islet Cells • Workshop 1: Single Cell Analysis of Islet Cell Constituencies • Inter-Organ Signaling Islet Biology• Immunology/Clinical Trials • Workshop 2: Big Data and Personalized Approaches to Diabetes • Bioengineering/Encapsulation

Scholarship Application & Discounted Abstract Deadline: October 5, 2017Abstract Deadline: November 7, 2017Discounted Registration Deadline: December 7, 2017

February 4–8, 2018 | Keystone Resort | Keystone, Colorado | USA

Frontiers in Islet Biology and Diabetes

www.keystonesymposia.org/meetings | 1.800.253.0685 | 1.970.262.1230

a 501(c)(3) nonprofit educational organization

Note: Scholarships are available for graduate students and postdoctoral fellows and are awarded based on the abstract submitted. Submitting an abstract is an excellent opportunity to gain exposure for your work. Abstracts submitted by the abstract deadline will also be considered for short talks on the program.Upper image of mouse pancreatic islet courtesy of Jakob Suckale

Meeting Hashtag: #KSisletbio www.keystonesymposia.org/18B3

SUNDAY, FEBRUARY 4Arrival and Registration

MONDAY, FEBRUARY 5Welcome and Keynote Address *Matthias Hebrok, University of California, San Francisco, USARonald M. Evans, The Salk Institute for Biological Studies, USANuclear Receptors, Diabetes and Beyond

Beta Cell Development/Maturation *Lori Sussel, University of Colorado Anschutz Medical Campus, USADanwei Huangfu, Memorial Sloan Kettering Cancer Institute, USAHuman Pancreas Development Through the Lens of Pluripotent StemCellsChris Wright, Vanderbilt University Medical Center, USAPancreatic Endocrine-Cell Birth: Focal Cell-IntrinsicNeurog3-Independent Morphogenetic Programs Linked to EpithelialFeedback, and Lineage-Priming as a Progenitor-PartitioningMechanismJoon Ho Moon, Korea Advanced Institute of Science & Technology,South KoreaShort Talk: Serotonin Regulates β-cell Proliferation during PerinatalPeriodRoland W. Stein, Vanderbilt University Medical Center, USARecruitment of the Swi/Snf Chromatin Remodeling Complex by Pdx1Is Required for Regulating Pancreas Development and Adult Islet βCell Function in vivoCheng-Ran Xu, Peking University, ChinaShort Talk: Deciphering Pancreatic Islet β-Cell and α-Cell MaturationPathways and Characteristic Features at the Single-Cell Level

Human Islet Biology *Roland W. Stein, Vanderbilt University Medical Center, USAAlvin C. Powers, Vanderbilt University School of Medicine, USALessons from Early Human Pancreatic Islet Development and FunctionAnath Shalev, University of Alabama at Birmingham, USAClinical Trials Promoting beta Cell SurvivalPeter C. Butler, University of California, Los Angeles, USAThe Mitochondrial Network and Function in Beta Cells in Health andType 2 DiabetesMarta Perez-Alcantara, University of Oxford, UKShort Talk: Human Islet Differentiation Model HighlightsDevelopmental Mechanisms Contributing to Type 2 Diabetes Pathology

Poster Session 1

TUESDAY, FEBRUARY 6Alpha Cells/Intracellular Islet Communication *Seung K. Kim, Stanford University, USAPatrick MacDonald, University of Alberta, CanadaAlpha Cell Function and Identity in Human T2D

Lori Sussel, University of Colorado Anschutz Medical Campus, USARegulation of Islet Cell Fates by Long Noncoding RNAsFrancis C. Lynn, University of British Columbia, CanadaExpression of Npas4 in Activated β-cells Represses the α-Cell FateMark O. Huising, University of California, Davis, USAA Urocortin 3-Mediated Negative Feedback Loop Controls InsulinSecretion to Determine the Homeostatic Set Point for Blood GlucoseDiana E. Stanescu, University of Pennsylvania, Children's Hospital ofPhiladelphia, USAShort Talk: The Role of SLC38A5 in Pancreatic alpha CellDevelopmentMegan Capozzi, Duke University, USAShort Talk: α Cells Control β Cell Function

Emerging Technologies and Disease Modeling *Guy A. Rutter, Imperial College London, UKDanny Hung-Chieh Chou, University of Utah, USANovel Ultrafast-Acting InsulinAndrew F. Stewart, Mount Sinai School of Medicine, USASynergistic Increases in Adult Human Beta Cell Proliferation InducedBy Combined Small Molecule TreatmentClive N. Svendsen, Cedars-Sinai Regenerative Medicine Institute,USAUsing Organ-On-Chip Combined with Induced Pluripotent Stem Cellsto Model Human DiseaseAlice Anna Tomei, Diabetes Research Institute, USAShort Talk: Conformal Coating of Stem-Cell-Derived Insulin-SecretingCells for Transplantation in Confined Well-Vascularized Sites withoutImmunosuppression

Poster Session 2

WEDNESDAY, FEBRUARY 7Progenitor to Islet Cells *Markus Grompe, Oregon Health & Science University, USAReprogramming Approaches for Beta-Cell ReplacementAnil Bhushan, University of California, San Francisco, USAA New Paradigm for T1D PathogenesisMatthias Hebrok, University of California, San Francisco, USAFunctional Analysis of Human Stem Cell Derived beta CellsHeiko Lickert, Institute of Diabetes and Regeneration, GermanyBeta Cell Heterogeneity and MaturationFiona M. Docherty, University of Colorado Denver, USAShort Talk: Identification of Factors Involved in Maintenance andDifferentiation of Uncommitted Human Pancreatic EndodermCarolina Rosselot, Icahn School of Medicine at Mount Sinai, USAShort Talk: c-Myc Regulation of Adaptive Beta Cell Proliferation andExpansion: Impairment in Type 2 Diabetes

Workshop 1: Single Cell Analysis of Islet Cell Constituencies

* Session Chair † Invited but not yet accepted Program current as of April 25, 2022. Meal formats are based on meeting venue. For the most up-to-date details, visit https://www.keystonesymposia.org.

KEYSTONE SYMPOSIAon Molecular and Cellular Biology

Frontiers in Islet Biology and Diabetes (B3)February 4-8, 2018 • Keystone Resort • Keystone, CO, USA

Scientific Organizers: Matthias Hebrok, Seung K. Kim, Felicia Pagliuca and Anil BhushanSponsored by Novo Nordisk A/S

Discounted Abstract & Scholarship Deadline: October 5, 2017 / Abstract Deadline: November 7, 2017 / Discounted Registration Deadline: December 7, 2017

*Julie B. Sneddon, University California, San Francisco, USALineage Dynamics of Pancreatic Development at Single-CellResolutionCarmen Argmann, Icahn School of Medicine at Mount Sinai, USAIntegrative Analysis of Bulk and Single Cell Sequence Data of HumanInsulinomas to Reveal Beta Cell Mitogenic Candidate GenesJaeAnn Dwulet, University of Colorado Denver, USASubpopulations of β-cells Play a Disproportionate Role in MulticellularIslet DynamicsNathalie Groen, Leiden University Medical Center, NetherlandsExploring Human Pancreatic β-Cell Plasticity using Single-cell RNASequencingNicole A. Krentz, , CanadaSingle Cell Transcriptome Profiling of the Mouse and HumanEndocrine Pancreas LineagesYan Li, Case Western Reserve University, USAHigh-Throughput Single Cell Transcriptome Analysis and CRISPRScreen Identify Key Beta Cell-Specific Disease GenesNils Wierup, Lund University, SwedenMultiple Type 2 Diabetes Genes and Functional Genetic NetworksIdentified using Single-cell RNA-sequencing of Human Pancreatic Islets

Inter-Organ Signaling Islet Biology *Anil Bhushan, University of California, San Francisco, USAGuy A. Rutter, Imperial College London, UKRole and Regulation of Beta Cell PacemakersRohit N. Kulkarni, Joslin Diabetes Center, Harvard Medical School,USALiver-Derived Signals Regulating beta Cell FunctionSeung K. Kim, Stanford University, USAEvidence that Neuromedin U Is a Mammalian Decretin HormoneLimor Landsman, Tel Aviv University, IsraelShort Talk: Pancreatic Pericytes Support beta-cell Function in aTcf7l2-dependent Manner

Poster Session 3

THURSDAY, FEBRUARY 8Immunology/Clinical Trials *Felicia Pagliuca, Vertex Pharmaceuticals, USAAnette-Gabriele Ziegler, Helmholtz Zentrum München, GermanyPrevention, Identification and Treatment of Early-Stage Type 1DiabetesKevan C. Herold, Yale University, USAAutoimmune Diabetes and Endocrinopathies Induced by CheckpointInhibitors Everett H. Meyer, Stanford University, USACAR T-Cell Immunomodulation in DiabetesSahar Keshvari, University of Queensland, AustraliaShort Talk: Development of Interleukin-22-Based Therapy for Type 2Diabetes

Sushant Bhatnagar, University of Alabama at Birmingham, USAShort Talk: The Complement 1q-Like 3 Secreted Protein Acts as anInhibitor of Insulin Secretion from Pancreatic β Cells

Workshop 2: New Tools and Technologies for Diabetes Research *Olov Andersson, Karolinska Institutet, SwedenIn vivo Drug Discovery for Increasing the Number of IncretinExpressing Cells to Reinforce Glucose Control by the Gut-PancreasAxisRonan Russell, University California, San Francisco, USAGenome Editing in hPSCs Reveals an Important Role for MAFB inHuman Beta Cell FormationHeshan Peiris, Stanford University School of Medicine, USAConditional Genetics and Human Islet Studies to Discover DiabetesRisk Gene FunctionJonathan D. Douros, Duke University, USAVertical Sleeve Gastrectomy (VSG) Rapidly andWeight-Independently Enhances Intrinsic Cell Triggering andAmplifies Pathways for Insulin SecretionGregory Michael Ku, University of California, San Francisco, USAA Genetic Interaction Map of Insulin Production Identifies Mfi as aNovel Inhibitor of Mitochondrial FissionMasaya Oshima, INSERM, FranceViral-Like Infection Induces Human β Cell DedifferentiationSophie Bauer, TissUse GmbH, GermanyFunctional Coupling of Human Pancreatic Islet Microtissues and LiverSpheroids in a Microphysiological System: Towards a Novel Humanex vivo Model of Type 2 Diabetes

Bioengineering/Encapsulation *Matthias Hebrok, University of California, San Francisco, USATejal A. Desai, University of California, San Francisco, USANanoporous Thin Film Devices for Islet EncapsulationFelicia Pagliuca, Vertex Pharmaceuticals, USADevelopment of a Stem Cell-Derived Pancreatic Islet Cell Therapy forDiabetesCherie Stabler, University of Florida, USAEngineering Bioactive Encapsulation Platforms for Islet Transplantation

Meeting Wrap-Up: Outcomes and Future Directions (Organizers)

FRIDAY, FEBRUARY 9Departure

* Session Chair † Invited but not yet accepted Program current as of April 25, 2022. Meal formats are based on meeting venue. For the most up-to-date details, visit https://www.keystonesymposia.org.

KEYSTONE SYMPOSIAon Molecular and Cellular Biology

Frontiers in Islet Biology and Diabetes (B3)February 4-8, 2018 • Keystone Resort • Keystone, CO, USA

Scientific Organizers: Matthias Hebrok, Seung K. Kim, Felicia Pagliuca and Anil BhushanSponsored by Novo Nordisk A/S

Discounted Abstract & Scholarship Deadline: October 5, 2017 / Abstract Deadline: November 7, 2017 / Discounted Registration Deadline: December 7, 2017