fluoreszenz prospekt engl

7/28/2019 Fluoreszenz Prospekt Engl

1/12

Simply Brilliant!Fluorescence Microscopy made by Leica Microsystems


2/12

The time after one new development is the time before the next

Today, biomedical research is inconceivable without continuous progress in

fluorescence microscopy. New fluorescence technologies often initiated new discoveries and

soon became standard applications, such as immunofluorescence,

which is now a routine diagnostic technique. Further examples are confocal microscopy and

live cell examinations with GFP.

The invention of fluorescence microscopy ...

In 1932, Leica designed the first epifluorescence microscope, whose technology is still used

today. Then, in 1965, epifluorescence filter cubes were developed at Leica in

cooperation with Professor Ploem; they were adopted by other manufacturers and have

remained the generally accepted standard right up to the present day.

As Leica is in constant contact with leading research scientists and laboratories

all over the world, our instruments offer special customer benefits which we combine with

innovative technologies and the quality of Leica optics.

... and the revolution in light microscopy

The latest example of our pioneering spirit and probably one of the most fascinating

developments ever is the high-resolution Leica TCS 4Pi microscope,

which can image cell structures that were previously beyond the resolving power of

conventional light microscopy. Together with Professor Stefan Hell,

Director of the Max Planck Institute for Biophysical Chemistry in Gttingen, Leica Microsystems

has developed a method which leads to a substantial reduction of focal volume.

This improves the resolution along the optical axis three- to seven-fold,

opening completely new horizons for research scientists.

Unlimited Progress


3/12

If only all decisions were so simple

Despite the complexity of a fluorescence microscope, the criteria for its quality are

crystal clear. There are three main aspects for assessing any fluorescence microscope:

Optics, i.e. the brilliance and wealth of contrast of the images Ease of use, i.e. well thought-out operation concept

Practical orientation and application potential, i.e. a wide and flexible range of equipmentBrilliance: a visible difference

Leica microscopes have always been famous for their optics. And rightly so:

Leica images feature outstanding f luorescence, razor-sharp contours and breathtaking contrast.

The finest structures are clearly visible even when the excitation intensity is

attenuated; unique brilliance ensures top imaging precision.

Ease of use: when jobs get harder, tools must get easier

It may sound paradoxical, but the more difficult the scientific challenges, the easier the

microscope should be to use. This is the only way to be able to concentrate fully on the

subject of research and obtain quick results even in extremely time-consuming research series.

Practical orientation: reproducibility and precision for all applications

From climate chambers to sample manipulation to filter changers: all components

interact in perfect harmony, guaranteeing simple work routines, reproducible images and, most importantly,

the knowledge that you can rely a hundred percent on your results.

Brilliant, Convenient, Practical


4/12

Answers To Every Question

Fluorescence

stereomicroscopy and

macroscopy:

Select, sort and assess

in 3D fluorescence.

Our stereomicroscopes

allow 3D imaging of

specimens and therefore

precise manipulation of

fluorescing objects.The upright fluorescence

macroscopes, on the

other hand, offer parallax-

free precision and a free

working distance that is

long enough to enable

viewing of specimens

the size of a mouse.

Fluorescence microscopy:

Our range of fluorescence

microscopes is extremely

wide. From the DM1000

standard routine microscope

to the high-end research

microscopes DM6000 and

DMI6000, youll be sure tofind the right system for any

application: we offer an

extensive choice of different

cameras and software

solutions. All the systems

feature brilliant optics, so

theres no need to sacrifice

high precision whatever the

size of your budget.

Confocal microscopy:

The essential characteristics of

confocal microscopy are top image

quality through optical sections and

outstanding three-dimensionalreconstruction of data sets.

We offer many different configurations

in this area of microscopy including

the worlds first filter-free Spectral

Confocal Microscope.


5/12


6/12


7/12

Digital Photography and Data Analysis

Experimental fluorescence

techniques:

Fluorescence Recovery after

Photobleaching (FRAP),

Fluorescence Resonance

Energy Transfer (FRET),

Photoactivation,

Fluorescence LiftetimeImaging (FLIM),

Fluorescence Correlation

Spectroscopy (FCS), etc.

enable the representation of

molecular interactions and

dynamics, answering

questions on cell functions.

Data analysis:

The software-supported

quantitative evaluation of

image data e.g. for FCS

or FLIM round off the

scientific information

yield of the experiment.

An essential requirement for reliable data analysis is excellent optical imaging quality as guaranteed by our

digital cameras. Choose between various models to suit your application needs.

Besides actual image information, interest is increasingly focusing on the development of new experimentalfluorescence techniques for exploring cell functions. These require powerful software solutions that are

precisely matched to your specifications and perfectly tuned to our cameras and systems. Thus equipped,

you can rely on smooth and safe data analysis and management for all your projects.

Image processing:

In traditional fluorescence microscopy,

images frequently seem slightly

blurred due to the fact that information

coming from outside the focal planeis included in the image. Image quality

can be significantly enhanced by

processing with mathematical

algorithms (deconvolution).


8/12


9/12

Leica MZ16 FA: The worlds best fluorescence stereomicroscope

Fully apochromatic 16:1 optic system, 920x magnification,840 line pairs/mm resolution, 600 m visible linewidth

and 0.282 NA numerical aperture

Patented TripleBeam fluorescence technologyfor intensive fluorescence imaging

Motorized functions: Filter changer, zoom, dual iris diaphragm,

focus drive, UV shutter, digital display and automated controlof multifluorescence experiments.

Leica MacroFluo: The worlds only fluorescence macroscope

Parallax-free imaging for high-precision analysis, measurements,digital and video documentation particularly with multifocus

and deconvolution programs

Choice of apochromatic 6.3:1 or 16:1 zoomMaximum macroscopic resolution for documentation of experiments

on large living objects (mice): 340 Lp/mm with

the 6.3:1 and 336 Lp/mm with the 16:1 zoom

Leica DM Series: Setting new standards for upright microscopes

Wide range of upright microscopes for routine and researchapplications in fluorescence microscopy

Intuitive and ergonomic handling, one button changing ofcontrasting methods with automated adjustments of diaphragms,

prisms and intensity values

Brilliant fluorescence, up to 8 pixel shift-free filtercubes,Fluorescence Intensity Manager

Leica DMI Series: Most sophisticated inverted microscopes,

optimized for fluorescence

Innovative new microscope design with up to 4 ports, fluorescenceoptimized optics and unique handling comfort

Fast internal filter wheel for vibration free excitation of fluorochromeswithin milliseconds and excitation manager to balance different

fluorochrome intensities

Fluorescence Intensity Manager for attenuation of excitation lightintensity to avoid photobleaching or stress in living cells

Leica DM LFSA: Unique automated fixed stage microscopes

The ultimate solution for electrophysiology, patch clamp andinfrared microscopy

Apochromatic long working distance water immersion dip in UVIobjectives corrected from UV to infrared (3801000 nm)

Highest precision focussing, slim design and stable set upfor vibration-free working


10/12

Leica AS TP: New benchmark for micromanipulation

The synergy of the combined know-how and experienceof two leading suppliers in the field of micromanipulation

Integration of microscope- and micromanipulator functions intoone control element

Efficient and comfortable working without touching the microscope

Brilliant fluorescence for GFP-expressing transgenic organisms

Leica AS LMD: Contact and contamination-free Laser microdissection

Highest precision laser microdissection for collection and analysisof cells and area from tissues and cultured cells

Simultaneous laser cutting and fluorescence imagingPowerful software for automated detection, dissection and

collection of fluorescent stained cells

Leica AS MDW: Life Cell Imaging in 6 dimensions

Sophisticated integrated solution for z-stacking, multichannelfluorescence, time lapses with highest light efficiency and

image acquisition speed.

All state of the art components e.g. monochromator, fine focussing,high performance camera are perfectly synchronized via unique

speed board technology

Optimized environmental control allows the analysis of dynamicprocesses in living cells

Leica TCS SP2 AOBS: Revolutionary breakthrough

Filterfree spectral confocal microscope

AOBS the ultimate beamsplitter: freely programmable to anymultichroic beamsplitter specification with up to 8 sharp

selection bands

The AOBS in combination with the spectral detector gives highestemission collection efficiency resulting in unmatched spatial

and spectral resolution and brilliant image quality

Easy and intuitive handling of hardware and Leica Confocal Software

Leica TCS SP2 RS: Ultrafast point scanning confocal microscope

High speed resonant galvanometer driven point scanner withup to 8000 l ines/second allows imaging of fast dynamic processes

Uncompromised spatial resolution and excellent signal tonoise ratio gives outstanding performance

Simultaneous multi color imaging combined with transmitted lightdetection


11/12

Leica TCS SP2 MP: In-depth viewing

Multiphoton microscopy is advantageous for the examinationof thick specimens

Excitation occurs only in the focal volume which leads to reducedphototoxicity and is therefore ideal for live cell imaging, in addition

external detectors increase the fluorescence collection efficiency

Available turnkey lasers are intuitive and easy to use

Leica TCS 4Pi: Entering the nanoworld highest resolution microscopy

First commercial 4Pi system, providing a stable platformwith performance at theoretical limits

Breaks the axial resolution barrier and provides new insightsfor structural biology of live and fixed specimens

Leica DFC FX: Digital camera range for all requirements

in fluorescence microscopy

Wide range of fluorescence digital cameras optimized foranalysis and documentation

Highest sensitivity: short exposure time for fast image acquisitionand to prevent photobleaching

Optimized signal to noise ratio using peltier cooling elements

Leica CW4000: Solutions for cytogenetic applications

Advanced software modules for True High Resolution Karyotyping,FISH, CGH and MFISH

Intelligent image handling in Leica CW4000 Karyotyping software,saving time and increasing productivity

FISH Spot counting facility included in Leica CW4000 CytoFISH softwareProven solutions for Comparative Genomic Hybridization (CGH)

and Karyotyping (MFISH)

Leica FW4000: Fully integrated system for multidimensional

widefield imaging

Complete integration with Leicas DM Microscope and FluorescenceDigital Camera range for fully automated image acquisition

Easy to use and intuitive interfaceSuitable for simple fluorescence image acquisition through

to z stacking and time lapse experiments

Additional modules for 3D Deconvolution and visualizationfor the ultimate in image resolution


12/12

fluoreszenz prospekt engl

Documents