FlashGel™ Power Supply Instruction Manual

Lonza Rockland, Inc. www.lonza.com

scientific.support@lonza.com Scientific Support: 800-521-0390 Customer Service: 800-638-8174

Rockland, ME 04841

  1

Table of Contents

Safety Information 2

Maintenance 3

Specifications 4

Introduction 7

Operation Instructions 9

Troubleshooting 12

Ordering Information 15

Warranty and Liability Information 17

 

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  3

Use a high level of precaution with any electrical device. Before connecting the electrical supply, check to see if the supply voltage is within the range stated on the rating label, and see to it that the device plug be firmly seated. Place the unit in a safe and dry location; it must NOT touch any surrounding objects. Follow the safety precautions for chemicals / dangerous materials. If needed, please contact a qualified service representative or scientific.support@lonza.com.

Environmental Conditions

Ensure the instrument is installed and operated strictly under the following conditions: 75 kPa – 106 kPa Pollution degree: 2 Main supply voltage fluctuations up to ±10% of the normal voltage Temperature: 15C-40C Humidity: ≤95% relative humidity, non-condensing For indoor use only Altitude up to 2000m

Maintenance

Visually inspect the power supply prior to use for signs of wear, cracks or damage. Do not use if damage is found. There are no user serviceable parts contained in the FlashGel™ Power Supply.

  4

Specifications

Electrical Specifications Output Voltage / Inc.: 10 - 300V / 1VDC Output Current / Inc.: 10 - 400mA / 1mA Max. Watt: 60W Rated Voltage: 100 - 240 V, 50 - 60 Hz, 2A Output Type: Constant Voltage or Constant Current Control: Microprocessor controller Timer: 1 - 999 minutes with alarm, continuous Safety Device: No load detection; shrouded plugs and sockets Physical Specifications Terminal Pairs: 2 Pairs Display: 3 digit LED Construction material:

Polycarbonate housing & aluminum bottom plates

Unit Dimension: 140 x 191 x 84mm Weight: ~1 kg

Catalog Number 57068 - FlashGel™ Power Supply

Front view Rear view

Main Power Jackand

Fuse Holder

Fan

ON/OFFSwitch

  5

Warning The FlashGel™ Power Supply has been tested and found to comply with the limits of CE

regulation. The power supply is marked with ETL which indicates it conforms to US and

Canadian product safety standards. The device also complies with RoHS, indicating it’s

free of certain hazardous substances associated with electrical and electronic equipment

which meets the environmental directive. These limits are designed to provide

reasonable protection against harmful interference when the instrument series is

operated in a commercial environment. This instrument used together with the

FlashGel™ Dock or other electrophoresis chamber, uses, and can radiate, radio

frequency energy, and if not installed and used in accordance with the instruction manual,

may cause harmful interference to radio communications. Operation of this instrument in

a residential area is likely to cause harmful interference in which case the user will be

required to correct the interference at their expense. Changes or modifications not

expressly approved by the party responsible for compliance could void the user’s

authority to operate the equipment. It is strongly recommended for the user to read the

following points carefully before operating this equipment.

1. Read and follow the manual instructions carefully.

2. Do not alter the equipment. Failure to follow these directions could result in personal

and/or laboratory hazards, as well as invalidate any warranty.

3. Use a properly grounded electrical outlet with correct voltage and current handling

capacity.

4. Disconnect from power supply before maintenance and servicing. Refer servicing to

qualified personnel.

5. Do not use the unit if there is any sign of damage. Replace damaged parts.

6. Do not use in the presence of flammable or combustible material; fire or explosion

may result. This device contains components which may ignite such materials.

7. Refer maintenance and servicing to qualified personnel.

8. Ensure that the system is connected to electrical service according to local and

national electrical codes. Failure to make a proper connection may create fire or

shock hazard.

9. Use appropriate materials and operate correctly to avoid possible hazards of

explosion, implosion or release of toxic or flammable gases arising from overheated

materials.

10. The unit shall be operated only by qualified personnel.

 

  6

Avoiding Electrical Shock

Follow the guidelines below for safe operation of the unit.

The FlashGel™ Power Supply has been designed to utilize shielded wires thus

minimizing potential shock hazard to the user. Lonza recommends against the use of

unshielded wires.

To reduce risk of electrical shock:

1. In the event of solution spilling on the instrument, it must be dried out for at least 2

hours and restored to NORMAL CONDITION before each operation.

2. Never connect or disconnect wires loading from the power jacks when the red

indicator light of power switch is on.

3. WAIT at least 5 seconds after stopping a run before handling output leads or any

connected apparatus.

4. ALWAYS make sure that your hands, work area, and instruments are clean and dry

before making any connections or operating the power supply.

5. ONLY connect the power cord to a properly grounded AC outlet.

Avoiding Damage to the Instrument

1. Do not attempt to operate the device if damage is suspected.

2. Protect this unit from physical damage, corrosive agents and extreme temperatures

(direct sunlight, etc.).

3. For proper ventilation and safety concerns, keep at least 10 cm of space behind the

instrument, and at least 5 cm of space on each side.

4. Do not operate the unit out of environmental conditions previously listed.

5. Prior to applying any cleaning or decontamination methods other than in accordance

with manufacturer’s recommendation, users should check with the manufacturer’s

instructions to see if the proposed method will damage the equipment.

Equipment Operation

Follow the guidelines below for safe operation of the unit:

1. NEVER access dangerous chemicals or other materials to reduce the likelihood of

possible hazard of explosion and damage.

2. Do not operate the unit without lids or covers.

3. A temporary conductivity caused by condensation might occur even though this

series is rated Pollution Degree 2 in accordance with IEC 664.

  7

Introduction

The Lonza FlashGel™ Power Supply completes the FlashGel™ System. The

FlashGel™ Power Supply is designed for use with the FlashGel™ Dock and extends its

capabilities outside the FlashGel™ System to most general electrophoresis applications.

The FlashGel™ Device Pack which includes the FlashGel™ Power Supply, FlashGel™

Dock, and FlashGel™ Camera offers an all in one comprehensive package for fast easy

nucleic acid separation. Its compact size, with two terminal pairs which provide sufficient

and accurate output voltages, can deliver reliable experimental results from one

experiment to another. Lonza’s FlashGel™ Power Supply is ETL (US and Canada),

RoHS and CE compliant to address environmental and safety concerns.

FlashGel™ Device Pack

For additional information on the FlashGel™ System visit: www.flashgel.com

FlashGel™ Dock 

FlashGel™ Power Supply 

FlashGel™ Camera 

  8

Product Description & Feature

The FlashGel™ Power Supply is powerful enough to provide the constant 225V or 275V

output, which is recommended to run the FlashGel™ Cassettes while being a fraction of

the size of power supplies with similar outputs. Continuous or timed operations are easily

performed using the simple and user-friendly interface. The FlashGel™ Power Supply

features 2 electrode pairs, allowing for up to 2 FlashGel™ Cassettes to be run

simultaneously, saving both time and valuable bench space. With a universal voltage

rating, the FlashGel™ Power Supply is also designed and constructed to rigorous safety

standards.

 

 

 

- Compact size

- Power capacity: 60W, 400mA, 300V

- Constant Voltage or Constant Current operation

- 1 V step voltage selection; 1 mA step current selection

- Timer

- No load detection

- Shrouded plugs and sockets

- Two pairs of outlet terminals

- Output voltage stability

- CE marked, ETL (conforms to US and Canadian product safety standards)

 

 

 

 

 

 

  9

Operation Instructions

Control interface

  

You will find six buttons and four LED indicators on the faceplate.

The LED indicates the status of the unit.

a. Setup Mode (before pressing RUN/Start)

LED light indicates your current parameter. If you are setting a value for Voltage, the

Voltage LED will be lit.

b. Operation Mode

After you start the experiment, the LED light next to the RUN/STOP button will light

up to indicate the unit is under operation.

1. – Select voltage value

2. – Select current value

3. – Timer button

4. – Increase the Voltage, Current or Time

5. – Decrease the Voltage, Current, or Time

6. – Start or stop the unit

  10

Start the operation

NOTE: To operate under constant voltage or constant current modes, adjust the other

parameter to the maximum value. For example, to operate under constant voltage,

adjust current to max before running using constant voltage, and vice versa.

1. Place the unit on a sturdy and level surface in a safe, dry place, away from laboratory

traffic.

2. Ensure that the AC power switch is OFF, and then plug the three-pronged power cord

into a grounded three-prong AC outlet with appropriate voltage (100V to 240V as

indicated on the rating sticker near the AC cord on the back of the unit).

3. Power on the unit by pressing the ON/OFF switch.

4. Connect the DC output jacks from the FlashGel™ Dock or other electrophoresis unit;

insert the red lead (+) into the red output jack, and the black lead (-) into the black

output jack.

5. Constant voltage or current

*To set constant voltage, press , the LED indicator will light up.

*To set constant current, press , and LED indicator will should light up.

6. Adjust the output value by pressing the or button.

*When running FlashGel™ Cassettes, refer to the FlashGel™ Dock instruction

manual for recommended electrophoresis conditions.

7. After adjusting your constant mode output value, switch to the other parameter and

set it to maximum.

8. Timer Setting: After setting the current or voltage output value, press the Timer button,

and then press button or button to adjust timer accordingly.

  11

Note: When timer is set as “0”, it means continuous operation.

9. Press button to start the run. Once the electrophoresis starts, the LED light

next to the button will light up.

10. Press button again to stop the unit at any time if necessary.

11. When the run is completed, Press button to terminate a timed run, and turn

the AC power OFF by the switch on the rear.

12. The programmed settings will be automatically saved into the system. The next time

you turn it on, the same settings as your previous experiment will be displayed.

  12

Troubleshooting

Many operating problems may be solved by carefully reading and following the

instructions in this manual. Some suggestions for troubleshooting are given below.

Should these suggestions not resolve the problem or if you have any questions, contact

Lonza Scientific Support or the Lonza distributor in your region for assistance. If

troubleshooting service is required, please include a full description of the problem.

Problem Possible Cause Potential Solution

No Display / lights

No AC power

Check if the power supply is

unplugged, or AC power source

problem

AC power cord is not

connected

Check AC power cord connections at

both ends. Use the correct cords.

The fuse has blown Replace the fuse

Repeated fuse

broken Hardware failure Contact Lonza Scientific Support

Operation stops

Electrophoresis

leads are not

connected to the

power supply or to

the electrophoresis

unit(s), or there is a

broken circuit in the

electrophoresis cell

Check the connections to the power

supply and on your electrophoresis

cell to make sure the connection is

intact; check condition of wires in

electrophoresis unit. Close the circuit

by reconnecting the cables. Press

START/STOP to restart the run.

High resistance due

to tape left on a

pre-cast gel,

incorrect buffer

concentration, or

incorrect buffer

volumes in the

electrophoresis cell*

Correct the condition by making sure

the tape is removed from the precast

gel, buffers are prepared correctly,

and the recommended volume of

buffer is added to the electrophoresis

unit.

  13

Er1

Error message

Over current(400mA

limitation reached)

Check if the buffer concentration is

appropriate. Excessive buffer

concentration may cause over current

issue.* To clear the error message,

press the START/STOP button again

Er2

Error message

Over voltage(300V

limitation reached)

Press START/STOP button to clear

the error message. Contact Lonza

Scientific Support if the problem

persists.

Er3

Error message

Thermal limitation

reached(Output

voltage <10V)

(1) Check the connections

(2) If Er3 error message persists, the

problem may be caused by internal

fan failure. Contact Lonza Scientific

Support.

nLD

Message

No load is detected (1) Check the connections

(2) Check the buffer condition / buffer

level.

AL1

Alarm message

Max. watts(60W) of

power reached

Warning message for reference

*Not likely when used with FlashGel™ Dock

Encountering Problems

1. Check the troubleshooting section.

2. Call Scientific Support or e-mail scientific.support@lonza.com

  14

Replacing the Fuse

For additional fuses, call Lonza Scientific Support or e-mail

scientific.support@lonza.com

To replace the fuse:

1. Turn off the main power switch at the rear of Power Supply and detach the power

cord.

2. Open the fuse compartment located inside the Power Entry Module by inserting a

small flat blade screwdriver into the slot below the ON/OFF switch. Turn the

screwdriver to gently pry open the fuse compartment.

NOTE: The fuse compartment will not open with the power cord in place.

3. Pull the fuse holder out of the compartment and inspect the fuse. If the fuse is burned

or there is a break in the fuse element, replace the fuse with the identical type of fuse

(T2AH250V) as provided in the fuse holder (see figure below).

4. Place the fuse holder back into the compartment.

5. Snap the cover closed.

  15

Ordering Information

FlashGel™ Devices

Cat No. Description

57068 FlashGel™ Power Supply

57025 FlashGel™ Dock For use with all FlashGel™ Cassette types

57040 FlashGel™ Camera Includes Camera, hood enclosure USB cable, and FlashGel™ Capture

Software. For use with the FlashGel™ Dock.

FlashGel™ Device Packs

Cat No. Description

57062 FlashGel™ Device Pack Includes FlashGel™ Dock, FlashGel™ Power Supply, and FlashGel™ Camera

57069 FlashGel™ Power Supply Pack Includes FlashGel™ Dock and FlashGel™ Power Supply

57065 FlashGel™ Camera Pack Includes FlashGel™ Dock and FlashGel™ Camera

 

FlashGel™ Consumable Kits Cat No. Description

57063 FlashGel™ DNA Kit Includes FlashGel™ DNA Cassettes 1.2% 12+1 well single tier 9pk,

FlashGel™ Loading Dye, and FlashGel™ Marker 100 bp – 4 kb

57064 FlashGel™ Recovery Kit Includes FlashGel™ Recovery Cassettes 1.2% 8+1 well double tier 9pk,

FlashGel™ Recovery Buffer, FlashGel™ Loading Dye,

FlashGel™ QuantLadder, and Visualization Glasses

57024 FlashGel™ RNA Kit Includes FlashGel™ RNA Cassettes 1.2% 12+1 well single tier 9pk,

RNA Marker, Sample Buffer, and Molecular Biology Water

  16

Notes

 

  17

Warranty and Limitation of Liability

EXCEPT AS SET FORTH BELOW, LONZA ROCKLAND, INC. MAKES NO WARRANTY OF ANY KIND, EITHER EXPRESS OR IMPLIED, BY FACT OR LAW, OTHER THAN LONZA'S (I) OBLIGATION TO DELIVER PRODUCT COMPLYING WITH LONZA'S PUBLISHED SPECIFICATIONS (OR AS OTHERWISE REFERENCED IN THE CONTRACT) AND (II) IMPLIED WARRANTIES OF TITLE, FREEDOM FROM ENCUMBRANCE, AND RIGHT TO TRANSFER SAME. LONZA MAKES NO WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE, OR WARRANTY OF MERCHANTABILITY OTHER THAN AS STATED HEREIN.

Lonza Rockland, Inc. warrants this product to be free from defects in materials and workmanship under normal service for one year from date of shipment, subject to the exclusions set forth below and provided that the product has been operated and maintained according to the instructions provided. If the product proves defective during this period, Lonza Rockland, Inc., will repair or replace it at our option, free of charge, if returned to us postage prepaid. This warranty does not cover: Consumable parts (fuse), damage in transit, damage caused by carelessness, misuse or neglect, normal wear through frequent use, damage caused by solvent corrosion, damage caused by improper handling or user alteration, nor unsatisfactory performance as a result of conditions beyond Lonza’s control.

LONZA ROCKLAND INC.'S TOTAL LIABILITY AND BUYER'S EXCLUSIVE REMEDY FOR ANY CAUSE OF ACTION ASSOCIATED WITH THIS PRODUCT, WHETHER BASED IN TORT, CONTRACT, STRICT LIABILITY OR ANY OTHER LEGAL THEORY IS EXPRESSLY LIMITED TO REPLACEMENT OF NONCONFORMING PRODUCT OR PAYMENT IN AN AMOUNT NOT TO EXCEED THE PURCHASE PRICE OF THE SPECIFIC PRODUCT FOR WHICH DAMAGES ARE CLAIMED, AT LONZA'S OPTION. LONZA ROCKLAND, INC., SHALL IN NO EVENT BE LIABLE FOR ANY OTHER DAMAGES INCLUDING, WITHOUT LIMITATION, INCIDENTAL, SPECIAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES, INCLUDING WITHOUT LIMITATION, LOST PROFITS, LOSS OF INCOME, LOSS OF BUSINESS OPPORTUNITIES, LOSS OF USE AND OTHER RELATED DAMAGES, HOWEVER CAUSED, NOR ANY DAMAGE ARISING FROM THE INCORRECT USE OF THE PRODUCT.

License and Trademark Information Some components and technology of the FlashGel™ System are sold under licensing agreements. The nucleic acid stain in this product is manufactured and sold under license from Molecular Probes, Inc. and the FlashGel™ Cassette is sold under license from Invitrogen IP Holdings, Inc. and is for use only in research applications or quality control, and is covered by pending and issued patents. Dark Reader is a trademark of Clare Chemical Research, Inc. The FlashGel™ Dock technology contains Clare Chemical Research, Inc. Dark Reader® transilluminator technology and is covered under US Patents 6,198,107; 6,512,236; and 6,914,250. The electrophoresis technology is licensed from Temple University and is covered under US Patent 6,905,585.

All trademarks herein are marks of the Lonza Group or its affiliates. For Research Use Only. Not for use in diagnostic procedures. Manufactured for Lonza © 2012 Lonza Rockland, Inc.

 

Lonza Rockland, Inc. www.lonza.com

scientific.support@lonza.com Scientific Support: 800-521-0390 Customer Service: 800-638-8174

Document # 00525706-0812-2 Rockland, ME 04841