ISSN 0974-0775

Bimonthly

(International Journal of Applied Agricultural & Horticultural Sciences)

GREEN FARMING

July-August 2016

CONTENTS

Number 4Volume 7

Bi-plot analysis of drought tolerance indices in bread wheat ( L.)

Association analysis of F population of rice for yield and bran oil content

Correlation and path analysis studies in rice ( L.) genotypes of India

Genetic diversity, variability and association analysis for yield traits in traditional rice ( )cultivars of southern Karnataka

Combining ability studies in rice ( L.) for yield and its component characters

SSR marker aided parental polymorphism survey for stigma exsertion in maintainer lines of hybrid rice

Combining ability analysis for yield attributing characters in Indian mustard [ (L.) C. & C.]

An assessment of combining ability and heterosis for yield and yield attributes in sorghum [ (L.) M.]

Character association and path coefficient analysis in bread wheat ( ) under late sown condition

Genetic variability, correlation and path analysis for yield & its components in horsegram ( )

Exploitation of hybrid vigour for identification of superior crosses in greengram [ (L.) Wilczek]

Studies on general and specific combining ability in sesame ( L.)

Correlation and path analysis for seed yield in clusterbean [ (L.) T.] genotypes

Correlation and path analysis for yield & yield related traits in French bean ( ) in Karnataka

Assessment of genetic variability and diversity in tomato ( M.) germplasm

Heterosis for yield and yield related components in tomato ( L.)

Genetic diversity for yield and quality components in tomato ( L.)

Variability and path coefficient studies in chilli ( L.)

Triticum aestivum

Oryza sativa

Oryza sativa

Oryza sativa

Brassica Juncea

Sorghum bicolor

Triticum aestivum

Macrotyloma uniflorum

Vigna radiata

Sesamum indicum

Cyamopsis tetragonoloba

Phaseolus vulgaris

Lycopersicon esculentum

Solanum lycopersicum

Solanum lycopersicum

Capsicum annuum

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SUNIL KUMAR JATAV and V.S. KANDALKAR

P. MAHESH BABU, Y. CHANDRAMOHAN and V. RAVINDRA BABU

A.K. MEENA, J. SURESH, CH. SURENDAR RAJU and H.P. MEENA

T.C. SRIDHAR, B.M. DUSHYANTHA KUMAR, B.R. MANI and G.K. NISHANTH

RITA R. PATEL and P.B. PATEL

K. SRUTHI, K.B. ESWARI, K.B. KEMPARAJU, K.JAYARAMULU and M. SHESHU MADHAV

JITENDRA MEENA, HARSHA, USHA PANT and RAM BHAJAN

S.K. JAIN and P.R. PATEL

DINESH KUMAR, NILANJAYA and CHANDAN KUMAR

RAKESH ALLE, V. HEMALATHA, K.B. ESWARI and V. SWARNALATHA

ANIL KUMAR, RAVI KANT, S.K. SINGH and CHANDRA MOHAN SINGH

A.R. PHADTARE, B.C. NANDESHWAR, R.M. BACHKAR and P.B. WADIKAR

SUKHDEEP SINGH SIVIA, G.S. DAHIYA, BALDEEP SINGH, A.K. DEHINWAL and BHAGWAT SINGH

HEENA M.S., DEVARAJU and RAVI C.S.

SHWETA, DUSHAYANTHA KUMAR B.M., VISHNUTEJ ELLUR and SANJEEVAKUMAR PATIL

B. RAJASEKHAR REDDY, ANIL K. SINGH, A.K. PAL, G. ESWARA REDDY and M.P. REDDY

B. RAJASEKHAR REDDY, ANIL K. SINGH, A.K. PAL, G. ESWARA REDDY and M.P. REDDY

SUNNIA GORKA, SANJEEV KUMAR, R.K. SAMNOTRA and PUJA RATTAN

3 ( L.)Oryza sativa

Contd. ....

Previous issue :

Vol. No. pp. 507-7597, 3,Research Papers

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NAAS Rating : 4.79

(Abbreviation : )Green Farming Int. J.

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Correlation studies in [ (L.) M.]

Genetic divergence analysis of locally collected genotypes of pointed gourd in Chhattisgarh region

Effect of different varieties and planting date on quality & yield of knolkhol ( var. Gongylodes)

Effect of seeding rates and weed management on weed dynamics, crop productivity and productioneconomics of urdbean

Influence of different plant spacing, varieties and fertilizer levels on yield, economics and quality of soybean

Effect of detopping practice on yield and yield components of maize ( L.) hybrid DHM 117

Nutrient budgeting in continuous rice-rice cropping system under long-term integrated nutrient management

Effect of tillage practices on productivity potential, economics and energetic of crops inrice-based cropping system

Impact of decomposition & N- release rate of organic manures on yield & chemical composition of maize ( )

Effect of integrated nutrient management on growth, yield & economics of rainfed finger millet ( )

Effect of nutrient management practices through organics on growth, yield and economics ofchickpea under rainfed condition

Effect of phosphorus and sulphur levels on the growth and yield of chickpea ( L.)

Effect of organic and inorganic nutrient sources on growth, quality and yield of turmeric ( L.)

Narrow spacing coupled with higher fertilizer doses increase cotton yield under rainfed condition

Effect of split & foliar application of organics on productivity and quality of scented rice ( L.)

Incidence and bio-rational management of black gram pod borer complex with lufenuron and its non-target toxicity

Status and diversity of hymenopteran parasitoid complex on major pests of pigeonpea in protectedand unprotected condition

Characterizing diversity of pv. causing bacterial blight of pomegranate

Mitigation and adaptation strategies for climate change : Extension professionals’ responsiveness

Effect of date of sowing, different seed treatments on seed germination & rootstock raising in ( )

Improvement in productivity by scheduling magnesium and boron fertilizers in Southern Laterites of Kerala, India

bhendi Abelmoschus esculentus

Brassica oleracea

Zea mays

rabi

Zea mays

Eleusine coracana

Cicer arietinum

Curcuma longa

Oryza sativa

Xanthomonas axonopodis punicae

ber Z. mauritiana

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P. RAJKUMAR and V. SUNDARAM

PUSHPENDRA SINGH, T.K. PANIGRAHI, G.L. SHARMA and T. TIRKEY

A.H. CHAUDHARI, J.R. VADODARIA and H.T. PATEL

AJAYYADAV, ASHISH DWIVEDI, RAGHUVIR SINGH, S.S. TOMAR, VINEET KUMAR and SUNIL KUMAR

G.S. KHAZI, W.N. NARKHEDE and S.K. NAYAK

B. MANJU BHARGAVI, B. MUKUNDAM, M. MALLAREDDY and Y. SIVALAXMI

S. SRIDEVI and M. VENKATARAMANA

TEJ RAM BANJARA, G.P. PALI, G.K. SHRIVASTAVA, SHRIKANT CHITALE and SUNIL KUMAR

N.N. CHAUDHARY, S.V. RATHOD, MAHESH CHANDAGARWAL and V.R. BHATT

M. THIMMAIAH, M. DINESH KUMAR, M.S. NANDISH and H.K. VEERANNA

KIRAN, SATYANARAYANARAO and C. RAMESHKUMAR

MUKESH KUMAR PANDEY, AJAY VERMA and PRAMOD KUMAR

K. RAVINDRA KUMAR, S. NARASIMHA RAO and N. RAJA KUMAR

S.D. HIWALE, MANGALA GHANBAHADUR and V.K. KHARGKHARATE

BHUNESHWAR PRASAD PURAME and SHRIKANT CHITALE

P.K. SARKAR and D. ROY

SOUMYA SHETTAR, SUSHILA NADAGOUDA, A.G. SREENIVAS and SHANKAR MURTHY M.

ADESH KUMAR, TANJEET SINGH CHAHAL, MANDEEP SINGH HUNJAN, P.P.S. PANNU and HARMINDER KAUR

B.S. GHANGHAS, P.S. SHEHRAWAT and JOGINDER SINGH MALIK

SHABBER HUSSAIN, M. JAMWAL, P. TUNDUP, JAHANGEER A. BABA and M.A. BHAT

EMIL JOSE and USHAMATHEW

Contd. ....

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Soil test based integrated nutrients requirement for cauliflower in Verisol of Chhattisgarh

Effect of graded levels of Zn in combination with or without microbial inoculation on Zn transformation in soil,yield and nutrient uptake by maize for red soil

Influence of integrated effect of inorganic and organic fertilizers with fly ash on soil properties and paddygrown on acid soil

Response of rice for micronutrients under different amendments in sodic soil

Studies on comparative efficiencies of seed priming, soil and foliar fertilization for Cu nutrition of wheat

Effect of mineral enriched composts on soil remineralization, yield & nutrient uptake in yardlong bean

Heavy metal contamination by waste disposal in soils of central Kerala

Effect of time of pruning on growth and yield parameters of guava cv. Sardar under different planting densities

Effect of retention of sprouts on bark and leaf yield in cinnamon ( J. Pres)

Phytochemical investigation of leaf of red L. using GC-MS

Effect of plant geometry and date of sowing on dry biomass yield of ( )

Pre-flowering pruning to ensure regular cropping and sustainable yield in litchi

Effect of pre-flowering defoliation on yield and quality of Thompson Seedless grape ( L.)

Yield parameters of kalmegh ( ) as influenced by INM practices in Pongamia based agri silvi system

Impact of integrated nutrient management on chlorophyll & nutrients concentration in strawberry leaf cv. Chandler

Performance evaluation and energy estimation of multipurpose tool carriers for tillage operations

Effect of green fodder on blood metabolic profile of large ruminants

Effect of defoliating chemicals on growth and flower yield of

Influence of extrusion processing parameters on composite flour-based extrudates

Effect of different concentrations of sapota pulp on the properties of ice-cream

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L.K. SRIVASTAVA, V.N. MISHRA, G.K. JATAV and VINAY SINGH

SUGANYAAYYAR and SARAVANANAPPAVOO

KIRAN PATEL, R.N. SINGH, V.K. SAMADHIYA, THANESHWAR KUMAR, K. TEDIA and PRIYANKA SHARMA

B.R. INIYALAKSHIMI, M. BASKAR, P. BALASUBRAMANIAM and T. RAMESH

NAVNEET JARYAL, J.S. MANCHANDA, SUNIL KUMAR and MUNISH SHARMA

S.V. SREEJA and K. USHAKUMARI

V. DIVYA VIJAYAN and P.K. SUSHAMA

RANI SHIRANAL, S.N. PATIL, KULAPATI HIPPARAGI, RAVINDRANATH NAIK and BALESH G.

R.G. KHANDEKAR, V.S. SAWANT, P.M. HALDANKAR and K.V. MALSHE

N.H. GARANIYA and A.H. BAPODRA

B.V. HIRPARA, J.R. PATEL, K.N. PRAJAPATI, D.H. PATEL, M.A. PATEL and A.D. PATEL

P.V. TAKAWALE, K. DAS, M.A. HASAN, B.C. DAS and S.K. DUTTA RAY

T.S. SHELKE, S.S. TAPKIR and T.S. MUNGARE

D. DIVYA BHARGAVI, A. MADHAVI LATA and A. SRINIVAS

BIJENDRA K. SINGH, AKHILESH K. PAL, ANIL K. SINGH and AKHILENDRA VERMA

RAMYA V., PRAKASH K.V., VEERANGOUDA M., DEVANAND MASKI and NAGESH KUMAR T.

KALYANI SARAP and S.D. CHAVAN

R. POORNA CHAITANYA, C. CHANDRASEKHARA RAO, V. SUCHITRA and D.R. SALOMI SUNEETHA

A.A. SAWANT, N.J. THAKOR, S.B. SWAMI and M.M. MALI

ANKITA MAKWANA, D.K. VARU and MITAL VAGHASIYA

Cinnamomum verum

Abrus precatorius

bhoy ambli Phyllunthus fraternus

Vitis vinifera

A. paniculata

Jasminum sambac

Strategic Vision Message : 34

Impact of rotary tiller in Indian agriculture : Overview� R. YADAV and G.M. VEGAD

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The development of hybrid rice breeding technology

involves improvement and evaluation of parental lines,

evaluation of the degree of heterosis for yield and techniques

for seed production. Customarily to produce hybrids on a

commercial scale, it is essential to change the function of

male and female reproductive systems of rice plants.

The low yield of F seed production, and thus the

availability of F seeds at reasonable prices, had been cited

as a major constraint to the wide adoption of hybrid rice in

countries outside China (Nguyen 2000, Tran 2002, Virmani

2003). The availability of affordable hybrid rice seeds to

farmers is crucial to the success of hybrid rice

commercialization since farmers have to use fresh hybrid

seeds in each crop season. The low yield of hybrid seed

production and high cost of hybrid rice seeds have been the

main complaints raised by seed producers and farmers, and

were listed as one of the major limitations in Asian countries

for hybrid rice extension.

In self pollinated crops like rice, hybrid breeding appeared

to be difficult, as the floral traits are unfavourable for

outcrossing. Use of male sterility system has immensely

helped in hybrid breeding. Virmani (1994) reported that

outcrossing is influenced by many floral traits like size of pistil

and stamen, stigma exsertion, angle of glume opening.

Among them, stigma exsertion is emphasized as a major

component in increasing pollination and seed set (Sheeba

2006). Stigma exsertion is an important trait that

contributes to the improvement of seed production in hybrid

rice and is closely related to seed productivity in hybrid rice

(Takano-kai 2011). Previous studies have

demonstrated that the stigma exsertion rate of the male

sterile line, the female parent in production of hybrid seed, is a

key factor contributing to the efficient improvement of hybrid

seed production, since exserted stigmas remain viable up to

about 4 days and could continue to accept pollens (Li

2004; Tian 2004). A male sterile line with high stigma

exsertion rate is expected to trap more pollen, thus improving

the efficiency of hybrid seed production. With an increase in

the frequency of stigma exsertion in male sterile lines of

hybrid rice, the seed-setting rate in hybrid seed production

1

1et

al.,

et al.,

et al.,

et al.,

ABSTRACT

To begin marker assisted selection or marker assisted back cross breeding for introgression of a desirable trait into a variety

from the donor genotype, study of parental polymorphism is a pre-requisite. Unless the parents are polymorphic for the traits

of interest, the further selection of plants carrying the traits of interest is not possible in the progenies. The experiment was

conducted in Directorate of Rice Research, Hyderabad. In the present study Rice microsatellite (RM) markers were used to

study the parental polymorphism between the selected two parents APMS-6B a popularly used maintainer line with low

stigma exsertion (14.95%) and BF-16B, another maintainer line with high stigma exsertion (80.25%). The two parents were

screened for parental polymorphism using 454 SSR markers, of which 118 markers exhibited polymorphism. The overall

polymorphism level for the surveyed SSR markers was 25.99 % across the 12 chromosomes. Construction of a Linkage Map

could be ensued procuring the generated genotyping data which could further avail QTL analysis and identification of

markers linked to stigma exsertion trait.

Key words : Hybrid rice, Parental polymorphism, Simple sequence repeats (SSR), Stigma exsertion.

INTRODUCTION

Received : 07 March 2015 ; Revised accepted : 26 May 2016

Green Farming Vol. (4) : 783-786 ;7 July-August, 2016

K. SRUTHI , K.B. ESWARI , K.B. KEMPARAJU , K. JAYARAMULU and M. SHESHU MADHAV1* 2 3 4 5

Department of Genetics and Plant Breeding, College of Agriculture, Prof. Jaya Shankar Telangana

State Agricultural University, Rajendranagar, Hyderabad - 500 030 (Telangana)

Research Paper

SSR marker aided parental polymorphism survey for stigma

exsertion in maintainer lines of hybrid rice

1

2 3 4 5

P.G. Student (Agric.) (kanneboinasruthi30@gmail.com),

Associate Professor, Scientist, Ph.D. Scholar, Senior Scientist

*

29

and the yield of hybrid seed also increased (Zetian and

Yanrong , 2010).

Microsatellites or simple sequence repeats (SSRs) are

widely used in rice genetic studies. SSRs are highly

polymorphic genetic markers and because of their

widespread distribution in the genome, high allelic diversity

and inherent potential have become a valuable source of

genetic markers. Identification of markers that are associated

with stigma exsertion and can be used in marker assisted

breeding for development of new parental lines with higher

outcrossing rate.

In order to map QTLs for stigma exsertion trait, parents

were first screened with DNA markers to establish parental

polymorphism survey between them. Parents selected for

polymorphism survey are with high stigma exsertion

maintainer line BF-16B and low stigma exsertion maintainer

line APMS-6B screened with the selected RM primers. In the

present study, Rice Microsatellite (RM) markers were used to

study the parental polymorphism between the selected

parents. About 454 RM markers located on 12 rice

chromosomes, based on the reported distribution.

Total genomic DNA was isolated from the parental lines

using the method of Zheng e (1991) with modifications.

Healthy leaf pieces (2-3 cm) from the youngest leaves of a 20-

25 day old rice seedlings were taken into the well of a spot test

plate (M/s Thomas Scientific, USA). 400 ml of extraction

buffer (50 mM Tris HCL, pH 8.0, 25 mM EDTA, 300 mM NaCl

and 1 % SLS) was added to the well. The leaf tissues were

macerated for about 15-20 seconds till it was completely

homogenized. Another 400 μl of extraction buffer was added

to the well containing the leaf sample. Using a micropipette of

1 ml capacity, the entire content from the well was transferred

to a 1.5 ml capacity micro centrifuge tube. About 400-500 μl

Phenol (pH 8.0) : Chloroform : Isoamyl alcohol (25:24:1) was

then added to the micro centrifuge tube. The contents were

mixed well by inversion for about 10 minutes and centrifuged

at 12000 rpm for about 10 minutes at room temperature. After

the centrifugation, the supernatant was aliquoted from the

micro-centrifuge tube without disturbing the intermediate

layer into a fresh 1.5 ml micro-centrifuge tube. To the clear

supernatant, 5-10 μl of RNAase (10 mg per ml) was added

and incubated for 45-60 minutes at room temperature (~ 37

C). After completion of the incubation period, about 500 μl of

Chloroform: Isoamyl alcohol (24:1) was added to the micro-

centrifuge tube and mixed well by inversion for 10 minutes.

The content was centrifuged at 12000 rpm for 10 minutes and

the supernatant was collected into another fresh 1.5 ml micro-

centrifuge tube without disturbing the intermediate layer. To

the clear supernatant equal volume (~500-600 ml) of chilled

isopropanol was added. The contents were mixed gently and

centrifuged at 12000 rpm for 10 minutes at room temperature.

The supernatant was drained gently without disturbing the

DNA pellet. About 150-200 μl of 70 % ethanol was added to

the pellet at the bottom of the micro-centrifuge tube. It was

ensured that the pellet was completely immersed in 70%

alcohol. Then the content was centrifuged at 5000 rpm for 5

minutes at room temperature. After centrifugation, the

supernatant was drained and 70% alcohol wash was

repeated once again. Finally, after centrifugation and draining

out the supernatant, the pellet was left overnight air drying at

room temperature. After complete drying of the pellet,

depending upon the size of the pellet, about 50-100 μl of

sterile distilled water was added for dissolving the pellet.

About 3 μl of the DNA sample was taken and loaded in 0.8 %

ethidium bromide stained agarose-TBE (concentration of

TBE) gel, electrophoresed for about an hour and the bands of

genomic DNA were visualized and documented in a UV gel

documentation system (M/s Alpha Innotech Corporation,

USA).

The genomic DNA of parental lines subjected to PCR

amplification as per the procedure described by Chen

(1997). .

PCR amplification was carried out in a thermocycler using

the temperature profile, followed by rapid cooling to 4°C prior

to analysis. For electrophoretic studies, The SSR-PCR

products were resolved in a 3%Agarose gel prepared in 0.5 X

TBE buffer stained with Ethidium Bromide. The gel was run at

a constant voltage of 90 V for 1 h and visualized under UV

light and documented using gel documentation system. The

temperature specifications for the denaturation of DNA

strands, Annealing of primers and extension steps are as

following .

The parental polymorphism survey indicated that a clear

polymorphism was observed between the parents. A total of

454 SSR primer pairs mapped on all the 12 chromosomes

were used for testing polymorphism between two parents.

MATERIALS AND METHODS

RESULTS AND DISCUSSION

DNAIsolation

Polymerase chain reaction

t al.

et al.

o

The total reaction was scaled down to 10 ΜI

Table 1

784 Sruthi et al.

30

Green Farming 7 (4)

Sr.No. Steps Temperature Time

1 Initial denaturation 94 C 5 min

2 Denaturation 94 C 30 s

3 Annealing 57 C 1 min

4 Extension 72 C 1 min

5 Final extension 72 C 7 min

6 Cooling 4 C A

°°°

°°

°

Table 1. Thermal profile for PCR reaction

118 SSR primer pairs exhibited polymorphism between

recipient parent APMS-6B and donor parent BF-16B and

remaining 336 were monomorphic. Percentage of

polymorphism highest ( ) on chromosome 3 (37.84)

and least on chromosome 9 (17.86). The average per cent

polymorphism on all the chromosomes was 25.99. The lack of

detectable polymorphism between two parents could be due

to the fact that both parents, APMS-6B and BF-16B, are

indica lines. Lack of molecular marker polymorphism

between indica genotypes has been earlier noticed in studies

by Xu . (2002) and Biradar (2004).

Polymerase Chain Reaction was done to check each

sample for parental polymorphism using specific Rice

Microsatellite (RM) primers as shown in . Out of

454 primers used the parents showed polymorphic only to

118 RM primers listed in .

The percentage of polymorphism was calculated using

the formula :

RM Primers Showing Polymorphism= -------------------------------------------------- x 100

The total number of RM primers used

The usage of molecular marker technology in breeding

programs has greatly increased its efficiency and fastens the

transfer of desirable genes among varieties and to introgress

novel genes from related wild species. Polygenic markers

which were previously difficult to analyze using traditional

breeding methods, would now be easily tagged using

molecular markers. The screening of markers for parental

polymorphism among the rice cultivars forms the basis for

tagging of the desired gene, fine mapping of the gene in the

rice chromosome and in the subsequent Marker Assisted

Selection (MAS) programmes. The polymorphic RM markers

can be used in the fine mapping of the stigma exsertion trait

and to study the mapping populations of crosses obtained

from these parents.

Table 3

Fig. 1 and 2

Table 2

et al et al.

31 Green Farming

July-August 2016

1 RM10018F 40 RM273 79 RM3845

2 RM1247F 41 RM17604 80 RM3480

3 RM10649 42 RM6770 81 RM210

4 RM10843 43 RM5633 82 RM22696

5 RM562 44 RM16720 83 RM5428

6 HRM11099 45 RM31 84 HRM22622

7 HRM11111 46 RM440 85 HRM22732

8 HRM11114 47 RM5140 86 HRM22977

9 RM5 48 RM169 87 RM23900

10 RM3642 49 RM188 88 RM23916

11 HRM10167 50 RM538 89 HRM24217

12 RM5536 51 HRM18222 90 RM105

13 RM208 52 HRM18270 91 HRM24654

14 RM279 53 HRM17950 92 RM4771

15 RM7485 54 RM20229 93 RM311

16 RM6509 55 RM19670 94 RM2504

17 HRM13238 56 RM20378 95 RM5095

18 HRM12469 57 RM510 96 RM216

19 RM15189 58 RM402 97 RM6673

20 RM15580 59 RM5463 98 HRM24941

21 RM85 60 RM1370 99 HRM25796

22 RM14303 61 RM133 100 RM229

23 RM7 62 HRM20060 101 RM21

24 RM15064 63 HRM20615 102 RM26669

25 RM7000 64 HRM20710 103 RM202

26 RM15741 65 RM21260 104 RM209

27 HRM14250 66 RM500 105 RM6327

28 HRM15337 67 RM3859 106 RM332

29 HRM15626 68 RM20897 107 RM26652

30 HRM15630 69 HRM20818 108 HRM25970

31 HRM15679 70 HRM21881 109 HRM26213

32 HRM15855 71 RM23386 110 HRM26829

33 RM16447 72 RM22612 111 RM155

34 RM16458 73 RM22971 112 RM179

35 RM16601 74 RM22416 113 RM7102

36 RM22554 75 RM7285 114 RM5746

37 RM16649 76 RM23643 115 RM28404

38 RM551 77 RM22837 116 RM27542

39 RM5979 78 RM5647 117 HRM28580

118 HRM28157

Table 2. Microsatellite markers exhibiting polymor-

phism betweenAPMS-6B and BF-16B

SSR marker polymorphism survey for stigma exsertion of hybrid rice 785

Fig. 1. Polymorphism between recipient parent APMS-

6B and donor parent BF-16B using RM21260

and RM5647 SSR primers

Fig. 2. Polymorphism between recipient parent APMS-

6B and donor parent BF-16B using RM4771 and

RM28157 SSR primers

32

786

CONCLUSION

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Monographs on Theoretical and Applied Genetics

in Proceedings of the 20

Session of International Rice Commission

Euphytica.

Rice Genetics

Newsletter.

95 :

40 :

61 :

22

127 :

8 :

th

Green Farming 7 (4)Sruthi et al.

Chrom-

Total no. No.of No.of Percentage of

1 40 12 28 30.002 32 6 26 18.003 37 14 23 37.844 39 12 27 30.775 30 9 21 30.006 39 11 28 28.217 32 6 26 18.758 55 16 39 29.099 28 5 23 17.8610 42 8 34 19.0511 44 11 33 25.0012 36 8 28 22.22

osome

of primers polymorphic monomorphic primers showing

No.

screened primers primers polymorphism

on each on each on each on each

chromosome chromosome chromosome chromosome

Table 3. Chromosome wise percentage of primers

showing polymorphism between parents,

APMS-6B and BF-16B