Figure. S1.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S1.1

Strains of L. monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637SK600

M39503ASK754

SK2642

SK2508N.a.N.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A58

0nm o

f cry

sta

l vio

let

0.0

0.1

0.2

0.3

0.4

0.5% NaCl (Ctr.)2.0% NaCl5.0% NaCl7.0% NaCl

Serotype 4bSerotype 1/2a

Figure. S1.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBTE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S1.2

L. monocytogenes strains

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637SK600

M39503ASK754

SK2642

SK2508N.a.N.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A5

80nm

of

crys

tal v

iole

t

0.0

0.2

0.4

0.6

0.8

1.0

1.2

0.5% NaCl (Ctr.)2.0% NaCl5.0% NaCl7.0% NaCl

Serotype 4bSerotype 1/2a

Figure. S1.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S1.3

L. monocytogenes strains

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637SK600

M39503ASK754

SK2642

SK2508N.a.N.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A5

80

nm

of

crys

tal v

iole

t

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

0.5% NaCl (Ctr.)2.0% NaCl5.0% NaCl7.0% NaCl

Serotype 4bSerotype 1/2a

Figure. S2.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S2.1

Strains of L. monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637SK600

M39503ASK754

SK2642

SK2508N.a.N.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A58

0nm

of

crys

tal v

iole

t

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

Glucose 0.25% (Control)Glucose 1.0%Glucose 5.0%Glucose 10.0%

Serotype 4bSerotype 1/2a

Figure. S2.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S2.2

Strains of L. monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637SK600

M39503ASK754

SK2642

SK2508N.a.N.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A58

0nm

of

crys

tal v

iole

t

0.0

0.2

0.4

0.6

0.8

Glucose 0.25% (Control)Glucose 1%Glucose 5%Glucose 10%

Serotype 1/2a Serotype 4b

Figure. S2.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S2.3

Strains of L. monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637SK600

M39503ASK754

SK2642

SK2508N.a.N.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A58

0nm

of

crys

tal v

iole

t

0.0

0.2

0.4

0.6

0.8

1.0

1.2

Glucose 0.25% (Control)Glucose 1%Glucose 5%Glucose 10%

Serotype 1/2a Serotype 4b

Figure. S3.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S3.1

Strains of Listeria monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A5

80nm

of c

ryst

al v

iole

t

0.00

0.05

0.10

0.15

0.20

0.25

0% Ethanol (Control)1.0% Ethanol2.0% Ethanol3.0% Ethanol5.0% Ethanol

Serotype 4bSerotype 1/2a

Figure. S3.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S3.2

Strains of Listeria monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A5

80

nm

of

crys

tal v

iole

t

0.0

0.1

0.2

0.3

0.4

0% Ethanol (Control)1.0% Ethanol2.0% Ethanol3.0% Ethanol5.0% Ethanol

Serotype 4bSerotype 1/2a

Figure S3.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S3.3

Strains of Listeria monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A5

80nm

of

dest

ain

ed c

ryst

al v

iole

t

0.0

0.2

0.4

0.6

0.8

1.0

0% Ethanol1.0% Ethanol2.0% Ethanol3.0% Ethanol5.0% Ethanol

Serotype 4bSerotype 1/2a

Figure S4.1. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 22.5oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S4.1

Strains of Listeria monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A5

80

nm o

f des

tain

ed c

ryst

al v

iole

t

0.0

0.2

0.4

0.6

0.8

1.0

1.2

Control (TSBYE)TSBYEgluc1%

TSBYENaCl2%

TSBYEgluc1%, NaCl2%

Serotype 4bSerotype 1/2a

Figure S4.2. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 30oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S4.2

Strains of Listeria monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A58

0nm

of d

esta

ined

cry

stal

vio

let

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

Control (TSBYE)TSBYEgluc1%

TSBYENaCl2%

TSBYEgluc1%, NaCl2%

Serotype 4bSerotype 1/2a

Figure S4.3. Absorbance (A580nm) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 37oC using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S4.3

Strains of Listeria monocytogenes

RM3373

RM3834

RM3835

RM4527

RM3023

RM 3316

RM 3349

RM 3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM 4515

RM3817

A58

0nm

of d

est

ain

ed c

ryst

al v

iole

t

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

1.8

Control (TSBYE)TSBYEgluc1%

TSBYENaCl2%

TSBYEgluc1%, NaCl2%

Serotype 4bSerotype 1/2a

Listeria monocytogenes strains

RM3373

RM3834

RM3835

RM4527

RM3023

RM3316

RM3349

RM3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM4515

RM3817

Gro

wth

rat

e (h

-1)

0.0

0.2

0.4

0.6

0.8

Control (TSBYE)TSBYEgluc1%

TSBYENaCl2%

TSBYEgluc1%+NaCl2%

Serotype 4bSerotype 1/2a

Figure S5.1. Maximum growth rate (h-1) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 22.5oC. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S5.1

Listeria monocytogenes strains

RM3373

RM3834

RM3835

RM4527

RM3023

RM3316

RM3349

RM3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM4515

RM3817

Gro

wth

rat

e (h

-1)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

Control (TSBYE)TSBYEgluc1%

TSBYENaCl2%

TSBYEgluc1%+NaCl2%

Serotype 4bSerotype 1/2a

Figure S5.2. Maximum growth rate (h-1) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 30oC. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S5.2

Listeria monocytogenes strains

RM3373

RM3834

RM3835

RM4527

RM3023

RM3316

RM3349

RM3354

RM4543

RM4561

SK1387SK90

SK1637

SK600

M39503ASK754

SK2642

SK2508N.a.N

.

SK1450

SK14032140

SK1495

SK1277

M35402A

M33027A

SK1463

RM 2387

RM2992

RM2998

RM4503

RM4504

RM4573

RM3013

RM 3302

RM4515

RM3817

Gro

wth

rat

e (h

-1)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

Control (TSBYE)TSBYEgluc1%

TSBYENaCl2%

TSBYEgluc1%+NaCl2%

Serotype 4bSerotype 1/2a

Figure S5.3. Maximum growth rate (h-1) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 37oC. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9).

FIG. S5.3