field portable gc/ms - technology and applications

Field-Portable GC/MSTechnology & applications

Dr. Joeri VercammenManaging Expert IS-X

www.is-x.com

http://www.is-x.com/

“GC/MS DEVICES”

Ease-of-use

Speed

Price

Sensitivity

Separation power

Identification of artefacts

Triple quadrupole GC/MSMS

Multipurpose autosampler

Ultratrace analysis

Runtime: 45 minutes

Expert knowledge required

RIVM“MOBILE CHEMICAL & BACTERIOLOGICAL LABORATORY”

Dimensions: 38 cm x 39 cm x 23 cm

Weight: < 14.5 kg

Power: LiH battery

Carrier gas: Helium cartridge

Sample prep/extraction

GC separation

MS detection

Data processing

1

3

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4

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GC separation

MS detection

Data processing

Solid Phase Microextraction (SPME)

Fiber coated with polymeric material

Expose to sample

Enrich components

Desorb in GC injection port

Solid Phase Microextraction (SPME)

First described in 1990 by J. Pawliszyn (Waterloo, Canada)

# 10.482 search results “SPME” in ScienceDirect

# 7.585 search results “SPME + food”

1995 1997 1999 2001 2003 2005 2007 2009 2011 2013 20150

100

200

300

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500

600

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Year

# P

ublicati

ons

CUSTODION-SPME

“More robust configurationof the classic SPME technique”

CUSTODION-NT

“Triple bed needle trap for dynamic sampling at low concentration”

CUSTODION-ADS

“Ideal for direct sampling ofvolatiles from surfaces”

1

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4


GC separation

MS detection

Data processing

Technology: Low thermal mass GC

Column: MXT-5, 5 m x 0.1 mm x 0.4 µm

Carrier: Helium @ 25 psig (EPC)

Injection: S/SL with Merlin head

Oven: 50°C (10 s) to 300°C (10 s) @ 2°C/s

R2R time: 5.00 min

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2

4


GC separation

MS detection

Data processing

Diameter: 4 mm

Trap heater: 150-180°C

Vacuum: 2-stage turbo + diaphragm

Ionisation: 70 eV

Mass range: 45 – 500 Da

Scan rate: 10 scans/s

T:\Research\data\F14\2011_03_09\00000488S.cdf

94

0.0

0.5e5

1.0e5

1.5e5

2.0e5

2.5e5

0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0

TIC(a

bundan

ce)

1

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2


GC separation

MS detection

Data processing

Each datafile is processed automatically with the

software. The algorithm is proprietary and identifies

peaks based on spectral matching & retention index.

DATA PROCESSING

START UP PROCEDURE

Switch instrument ON

Wait for idle (2.00 minutes)

Blank analysis: pass/fail?

Inject QC standard: pass/fail?

Start analyses

APPLICATIONS

Shelflife & freshness

Flavour & fragrance

Authenticity

Packaging

Toxins

Pesticides

…

In vivo SPME with in vitro calibration:Determination of off-flavor components in live fish

Z. Bai e.a., Anal. Chem. (1993) ,

1Strawberries

2.00 min

Ethyl acetate (87%)

Butanoic acid, ethyl ester (86%)

Hexanoic acid, ethyl ester (77%)

0 2 4 6 8 10 12 140.0E+00

5.0E+09

1.0E+10

1.5E+10

2.0E+10

2.5E+10

Butanoic acid, ethyl ester Butanoic acid, 2-methyl-, ethyl ester 1-Butanol, 3-methyl-, acetateHexanoic acid, ethyl ester Acetic acid, hexyl ester Benzoic acid, ethyl esterEthyl acetate Propanoic acid, 2-methyl-, ethyl ester

Time (Days)

2Chicken Filet

0 1 2 3 4 5 6 7 8 90.0E+00

5.0E+08

1.0E+09

1.5E+09

2.0E+09

2.5E+09

3.0E+09

3.5E+09

4.0E+09

4.5E+09>Disulfide, dimethyl >Dimethyl trisulfide

>Ethanol, 2-(2-ethoxyethoxy)- >Benzene, 2,4-diisocyanato-1-methyl-

>2H-Benzimidazol-2-one, 1,3-dihydro-5-methyl- >Pyridine, 4-(1-pyrrolidinyl)-

Time (Days)

3Pistachio Nuts

0 2 4 6 8 10 12 140.0E+00

1.0E+08

2.0E+08

3.0E+08

4.0E+08

5.0E+08

6.0E+08

7.0E+08

8.0E+08

9.0E+08

Butanal, 3-methyl- Butanal, 2-methyl- Hexanal

Ethanol, 2-(2-ethoxyethoxy)- 3-Carene

Time (Days)

KEY TAKE AWAYS

Easy-to-use field portable

GC/MS system with immediate

applicability in food-related

analysis.

Both ons-site as in the lab!