Field Evaluation of Host Plant Resistance in Strawberry Against Verticillium Wilt, Macrophomina

Crown Rot and Fusarium Wilt

G.J. Holmes, S. M. Mansouripour, J. Winslow, B. Gomez-Soto, B. Liu and T. Gordon

Orlando, FL

Pathology Automation

Entomology

NEXT-GEN DISEASE RESISTANCE BREEDING

1 = highest priority; 2 = medium priority; 3 = lowest priority; N = non-priority. SD = short-day, DN = day-neutral, and NP = nursery production.

R-gene = resistance gene, QTL = large-effect quantitative trait locus, complex = polygenic, complex genetics, and ? = unknown or hypothesized.

Portola Monterey

VERTICILLIUM TRIAL 2015

San Andreas Albion

Fusarium wilt

Natural

Cal PolyField 25

Verticillium wilt

Natural

Cal PolyField 35b

Macrophominacrown rot

Artificial

Monterey Bay AcademyLocation

Disease

Inoculum

90 genotypes30 cultivars60 elite lines4 replicates

14 DAP

Reading plant mortality every two weeks

Drone imaging every two weeks after planting

VERTICILLIUM WILT SUSCEPTIBILITY

VERTICILLIUM WILT SUSCEPTIBILITY

UC PSI Plan

asa

LC Driscoll’s

0

10

20

30

40

50

60

70

80

90

100

UC-

5DR

-8DR

-20

Man

resa

UC-

6DR

-7U

C-4

UC-

9U

C-14

Peta

lum

aM

arqu

isU

C-11

PS 9

271

DR-1

3DR

-11

Amad

oDR

-3PE

3.2

11PS

I-5DR

-17

DR-1

4Fr

onte

ras

PE 3

.221

PS 3

.108

Sens

atio

nDe

l Rey

UC-

15PE

6.2

036

PE 7

.205

9PS

I-6DR

-6PL

-02-

32Bi

g Su

rDR

-10

Cabr

illo

PSI-7

DR-1

6U

C-1

PL-0

5-10

0RBG

431

6En

chan

teU

C-13

Celin

ePS

I-8LC

-5U

C-12

Beau

tyPS

I-4U

C-10

UC-

16BG

3.3

24DR

-9O

sceo

laBG

4.3

67PL

-09-

11U

C-7

UC-

3LC

-2U

C-8

PS 5

016

BG4.

352

LC-8

PL-0

8-22

RU

C-2

LC-7

DR-1

Mav

eric

kDR

-19

PSI-2

DR-1

2El

yana

DR-5

PSI-1

DR-4

DR-1

5La

redo

Swee

t Ann

Win

ters

tar

FL 1

3.26

-134

Fest

ival

DR-1

8Ra

dian

ceBG

6.3

024

DR-2

LC-9

LC-3

PSI-3

LC-4

Ruby

June

Mon

tere

yLC

-6

Mor

talit

y (%

)

Cultivar/coded genotype

Verticillium Wilt - 2018

Marquis

Osceola

Festival

Verticillium Field

Week43

Week30

VERTICILLIUM TRIALJuly 15, 2017

Monterey (Vert-S)

0

10

20

30

40

50

60

70

80

90

100

30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul

Gre

en co

vera

ge(%

)

Date

Mor

talit

y (%

)

`

Manresa (Vert-R)

0

10

20

30

40

50

60

70

80

90

100

0

10

20

30

40

50

60

70

80

90

100

30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul

Gre

en co

vera

ge(%

)

Gre

en co

vera

ge(%

)

Date

=Manresa-R=Monterey-S

`

0

10

20

30

40

50

60

70

80

90

100

Mor

talit

y (%

)

Cultivar name

Verticillium wilt

2017 AVG = 30.12018 AVG = 11.6

∆ = 61.5%

March 1, 2017

MACROPHOMINA TRIAL

14 DAP

MACROPHOMINA CROWN ROTSUSCEPTIBILITY

MACROPHOMINA CROWN ROTSUSCEPTIBILITY

UCPSI Pl

anas

a

LC Driscoll’s

August 1, 2017MACROPHOMINA TRIAL

Festival

Fronteras

Manresa

0

10

20

30

40

50

60

70

80

90

100

DR-R

Lare

doU

C-K

PS 9

271

Osc

eola

UC-

MDR

-EDR

-KDR

-NDR

-HM

anre

saDR

-LU

C-L

DR-T

DR-D

DR-S

DR-J

Amad

oBG

6.3

024

UC-

iDR

-GPS

I-CG

rena

daPE

6.2

036

BG 4

316

PSI-D

DR-O

Big

Sur

Ench

ante

Sens

atio

nDR

-iBG

4.3

67M

arqu

isU

C-E

UC-

OU

C-N

DR-M

LC-B

Fron

tera

sCe

line

Mav

eric

kPE

7.2

059

UC-

JPL

-09-

11PS

I-HBG

4.3

52De

l Rey

DR-Q

DR-F

BG 3

.324

PSI-E

PSI-F

UC-

APE

3.2

11PS

I-BPe

talu

ma

PSI-G

PS 5

016

PL-0

2-32

PS 3

.108

DR-C

UC-

DDR

-PLC

-iU

C-F

Mon

tere

yLC

-GPE

3.2

21FL

13.

26-1

34Be

auty

Albi

onRa

dian

cePS

I-ALC

-HU

C-B

DR-A

UC-

CPL

-08-

22R

LC-E

Cabr

illo

Fest

ival

LC-C

UC-

GU

C-H

PL-0

5-10

0RSw

eet A

nnDR

-BRu

by Ju

neLC

-FLC

-D

Mor

talit

y(%

)

Cultivar/coded genotype

Macrophomina Crown Rot - 2018

OsceolaDel Rey

Ruby June

Verticillium Field Macrophomina Field

Week43

Week30

Ruby June (Mac-S)

0

10

20

30

40

50

60

70

80

90

100

30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul

Gre

en C

over

age

(%)

Date

Mor

talit

y (%

)

`

Osceola (Mac-R)

0

10

20

30

40

50

60

70

80

90

100

0

10

20

30

40

50

60

70

80

90

100

30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul

Gre

en C

over

age

(%)

Gre

en C

over

age

(%)

Date

=Ruby June-S=Osceola-R

`

0

10

20

30

40

50

60

70

80

90

100

Mor

talit

y (%

)

Cultivar name

Macrophomina crown rot

2018 AVG= 24.02017 AVG= 46.8

∆ = 48.7%

50

60

70

80

90

100

110

1-O

ct8-

Oct

15-O

ct22

-Oct

29-O

ct5-

Nov

12-N

ov19

-Nov

26-N

ov3-

Dec

10-D

ec17

-Dec

24-D

ec31

-Dec

7-Ja

n14

-Jan

21-J

an28

-Jan

4-Fe

b11

-Feb

18-F

eb25

-Feb

4-M

ar11

-Mar

18-M

ar25

-Mar

1-Ap

r8-

Apr

15-A

pr22

-Apr

29-A

pr6-

May

13-M

ay20

-May

27-M

ay3-

Jun

10-J

un17

-Jun

24-J

un1-

Jul

8-Ju

l15

-Jul

22-J

ul29

-Jul

5-Au

g

Tem

pera

ture

(F)

Date

Daily Max TemperaturePlantingTermination

Heat Wave

Water stress

50

60

70

80

90

100

110

1-O

ct8-

Oct

15-O

ct22

-Oct

29-O

ct5-

Nov

12-N

ov19

-Nov

26-N

ov3-

Dec

10-D

ec17

-Dec

24-D

ec31

-Dec

7-Ja

n14

-Jan

21-J

an28

-Jan

4-Fe

b11

-Feb

18-F

eb25

-Feb

4-M

ar11

-Mar

18-M

ar25

-Mar

1-Ap

r8-

Apr

15-A

pr22

-Apr

29-A

pr6-

May

13-M

ay20

-May

27-M

ay3-

Jun

10-J

un17

-Jun

24-J

un1-

Jul

8-Ju

l15

-Jul

22-J

ul29

-Jul

5-Au

g

Tem

pera

ture

(F)

Date

20172018

Fusarium oxysporum f.sp. fragariae

Monterey Bay Academy

FUSARIUM TRIAL 2015

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

Sweet Ann

Monterey

Radiance

Albion

Sabrina

Grenada

Sensation

Winterstar

Ventana

San Andreas 4.1Festival 2.3Portola 1.7

Safari 0.9Fronteras 0.6Petaluma 0.3

Percent Mortality on July 23, 2015

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

Sweet Ann

Monterey

Radiance

Albion

Sabrina

Grenada

Sensation

Winterstar

Ventana

San Andreas 4.1Festival 2.3Portola 1.7

Safari 0.9Fronteras 0.6Petaluma 0.3

Percent Mortality on July 23, 2015

Monterey: 75% mortality

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

Sweet Ann

Monterey

Radiance

Albion

Sabrina

Grenada

Sensation

Winterstar

Ventana

San Andreas 4.1Festival 2.3Portola 1.7

Safari 0.9Fronteras 0.6Petaluma 0.3

Percent Mortality on July 23, 2015

Albion: 53% mortality

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

Sweet Ann

Monterey

Radiance

Albion

Sabrina

Grenada

Sensation

Winterstar

Ventana

San Andreas 4.1Festival 2.3Portola 1.7

Safari 0.9Fronteras 0.6Petaluma 0.3

Percent Mortality on July 23, 2015

San Andreas: 4% mortality

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

Sweet Ann

Monterey

Radiance

Albion

Sabrina

Grenada

Sensation

Winterstar

Ventana

San Andreas 4.1Festival 2.3Portola 1.7

Safari 0.9Fronteras 0.6Petaluma 0.3

Percent Mortality on July 23, 2015

Portola: 2% mortality

FUSARIUM TRIAL 2015

0 1 1 2 2 410

16

2732 33

53 55

76

97

16

0 0 0 0 0

39

69

29

77

89

0

10

20

30

40

50

60

70

80

90

100

Fusarium wilt at MBA

2015 2016

% M

orta

lity

* * * *

* = not included in 2016 exp’t

CULTIVAR DISEASE RESISTANCE TABLE

Conclusions• Wide range of susceptibility among genotypes and

breeding program entries• High levels of resistance to Verticillium wilt, Fusarium

wilt and Macrophomina crown rot• Results consistent between years• Aerial images were equally effective to ground ratings

Acknowledgements

SCRI grant No.2017-51181-26833