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TRANSCRIPT
Copyright Cosucra 1
Feed
your
world
dr Loek Pijls
for Cosucra
Copyright Cosucra 2
Copyright Cosucra
Nice to meet
3
https://www.youtube.com/watch?v=Wrxxv_8rL1I&list=PL-UqyRlowYJwfQCN5fMIy-VEanGbzeJYC
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“Ask not (only) what your
microbes can do for you,
but (also) what you can do
for your microbes”
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Feed
your
world
• We eat
• Left-overs small-intestine -> enter large-intestine
• Dinner for microbes in large intestine
• Their metabolites:
• change colonic “weather”
• impact colonic cells - barrier
• are absorbed into & impact whole body
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WHAT ARE WE LOOKING AT?
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What are wheat AXOS?
AXOS = Arabino-Xylo-Oligo-Saccharides
From wheat bran - naturally sourced
Chain of xylose, substituted by arabinose &
containing ferulic acid units: anti-ox, anti-
inflammatory
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Origin of Fibruline chicory inulin & oligofructose
Fibruline® (inulin) & Fibrulose® (oligofructose): soluble fibres, gently extracted from the root of chicory (Cichorium intybus L.).
16%
7%
3%
Water
Inulin/oligofructose
Pulp
Other soluble matter
74%
Our chicories are locally grown in Belgium and North of France
We supply our farmers with our chicory seeds (Chicoline), and ensure good agricultural
practices
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What is chicory inulin? What is chicory inulin? What is chicory inulin?
• Linear chain of fructose units
• Feed for chicory leaves, blossom, fruit &
seeds - like breast milk for a baby
• Most well-established prebiotic
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From Belgium
12
Inulin & oligofructose in Europe
Spelt & poppy seed yogurt Danone, Austria 2016 Contains oligofructose
Source: Mintel -GNPD Wholegrain breakfast cereals Kellogg’s, Ireland 2016 Contains inulin as source of fibre
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Chicory inulin & AXOS
ProDigest VIB Bio-incubator Gent | Technologiepark 4
B-9052 GENT (Belgium)
tel. +32(0) 9/241.11.90 | fax. +32(0) 9/241.11.91
Part 1 • Bacterial faecal samples of three male Gent students • Incubation in “simulated large intestine” • Evaluate the effect of (5 g/L): Inulin: Fibruline Instant AXOS: arabinoxylan-oligosaccharide-enriched
compound, from wheat arabinoxylan Control: colon medium
Sampling at 0, 24 & 48 h Endpoints: pH Gas production Short-chain fatty acid
Part 2 Effects of metabolite mix on colonic cells • Permeability • Viability • Immune markers
SHIME: IN-VITRO SIMULATOR HUMAN INTESTINAL MICROBIAL ECOSYSTEM
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PH
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GAS
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ACETATE
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PROPIONATE
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BUTYRATE
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Barrier integrity (TEER)
Inflammation (cytokines & chemokines)
Macrophages - THP1-B
Colonocytes - Caco-2
Lipopolysaccharide from E. coli
Intestinal lumen
Blood stream
MODEL INFLAMMATORY BOWEL DISEASE
↓ incubation mix with metabolites
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EFFECTS ON COLONOCYTES
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IMMUNE MARKERS
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PRINCIPAL COMPONENT ANALYSIS: 5 -> 2
Inulin
Ctrl
Na-Butyrate
BNC
Axos
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•Cosucra’s Fibruline®: prebiotic; stimulation of
gut bacteria -> metabolites e.g. acetate,
propionate & butyrate
•Impacted markers of immunity/inflammation &
improve intestinal gut barrier function.
•May moderate leaky gut resulting from e.g.
food intolerances, allergies, Crohn disease
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