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DESCRIPTIONFaecal Egg. Count. Elisabeth Murdoch College Vet Science Students 2006. Materials- ~ 100ml beaker (Figure 3) ~ pipette ~ microscope (figure1) ~ Whitlock Universal counting slide *(figure2) ~ bucket ~ weigh scales ~ spatula ~ rubber gloves ~ 2kg of salt ~ 10ml syringe - PowerPoint PPT Presentation
Elisabeth Murdoch College Vet Science Students 2006
Materials-~ 100ml beaker (Figure 3)~ pipette~ microscope (figure1)~ Whitlock Universal counting slide*(figure2)~ bucket~ weigh scales~ spatula~ rubber gloves ~ 2kg of salt~ 10ml syringe~ plastic cup~ whisk
Figure 1Figure 2Figure 3* Click to see further details to purchase slides
Step #1Randomly collect 10 faeces samples from a mob of sheep. Step #2Weigh out 2 grams of each of the 10 samples into a plastic cup. You should end up with 20 grams in total.
NOTE: Rubber gloves should be worn!!!
Step #3Using a 10ml syringe add 10ml of water to the faeces you just weighed out.Step #4Mix faeces and water to a consistent mix resulting in minimal lumps.
Step #5Weigh out 2 grams of the newly made mix and add a further 9ml of water.Step #6Mix into a thin paste.
Step #7Add 50ml of saturated salt solution* to the thin paste and mix well. If you do not mix it well enough it can result in a false count.* Click to see further instructionsStep #8Stir vigorously using a pipette. Assure you stir back and forth as stirring in a circular motion will throw the eggs into the centre and can result in a false count.
Saturated salt solution-~ Poor bag of salt* into a clean bucket and fill full with cold water.~ Give it a good stir using a whisk before leaving it to sit for about 10 minutes.~ Using a syringe draw up 10ml of the solution.~ Place a beaker on the scales and zero them~ Squirt the solution in the beaker, it has to way as close to 12 grams as possible (anything above 11.8g is fine).~ If you are struggling to get it to weigh 12g give it a good stir or add more salt.~ Make sure your scales are accurate!!!
Back to steps*normal 2kg bag of sea salt from the supermarket, even Home Brand!
Step #9Draw up a sample, using either a pipette or a syringe (whatever is easier).Step #10Squirt the sample into the 2 middle cells of the Whitlock Universal Counting slide.
Step #11Place the slide under the microscope and set up microscope to suit.Step #12Count all the eggs between the ruled lines on the slides of both cells of the slide. Differentiate egg types* and tally the amount of eggs for each type of egg identified.* Click to see further information
Egg types-The main types of parasite eggs that can be found in sheep faeces include nematodes, trematodes and cestodes.
~Nematode species include: -Trichuris (fig 1) -Strongyle type worms* (fig 2)~Trematode species include: -Fluke (fig 5)~ Cestode species include: -Tapeworm (fig 4)*group of species not actual speciesBack to steps
Calculate faecal egg count. To find out how many eggs per gram you use the formula below:
epg= eggs counted x 30
This is the formula because the 2gram sub-sample was diluted to 60ml. So a ratio of 1:30 weight/volume was created. Each cell on the slide holds 0.5ml of the mix.
To purchase the Whitlock Universal Counting Slide see details below. JA Whitlock & Co PO Box 51, EASTWOOD NSW 2122, AUSTRALIA ABN 70 033 804 404-GST Registered phone (02) 9638 1142 fax : (02) 9638 1131 e-mail firstname.lastname@example.org website www.whitlock.com.au Back to materials list
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