Extraction of Peptides and

Amino Acids

Wim MulderSustoil, Foggia 24 April 2009

Composition oil cakes

Table 1. Composition of rapeseed, sunflower and olive pulp

Rapeseed Sunflower Olive pulp Water 5.0 6.7 50.0 Oil 41.6 53.3 22.0 Protein 26.2 22.9 1.6 Carbohydrates + fibres 23.4 14.0 24.9 Ash 3.8 3.1 1.5

Extraction of peptides and amino acids relevant for rapeseed and sunflower cake

Amino Acids

Nutritional value Taste Physiological/chemical characteristics

Production Chemical Enzymatic Fermentation Extraction

Human food

Animal feed

Oil cakes Hydrolysis

Protein hydrolysis

Complete hydrolysis 6 n HCl, 110°C, 24 hours 5 n NaOH, 100°C, 18 hours Salts incorporated Destruction amino acids

Enzymatic hydrolysis Peptides Immobilised enzymes

Extraction before oil recovery

rapeseed or sunflower seed

rest seedspeptides and amino acids

aqueous hydrolysis

oil

pressing

fractionated amino acids

Extraction after oil recovery

rapeseed or sunflower press cake

soluble proteinspeptides and amino

acids

acidic/alkaline extractionenzymatic hydrolysis

peptides and amino acids

aqueous hydrolysisfractionated amino acids

fractionated amino acids

Simultaneous extractionground oilseed

alkaline extraction centrifugation

solids: protein concentrateliquid

oil emulsion

acidic extraction centrifugation

3-phase centrifugation

liquid solids

3-phase centrifugation

oil

oil emulsion liquid

oil protein isolate

precipitation

ground oilseed

alkaline extraction centrifugation

solids: protein concentrateliquid

oil emulsion

acidic extraction centrifugation

3-phase centrifugation

liquid solids

3-phase centrifugation

oil

oil emulsion liquid

oil protein isolate

precipitation

Chemicals from amino acids

Biobased route for butanediol

Biomass

Proteins

Sugars

Amino acids

Other/rest amino acids

for food, feed or

chemicals

1,4-buanediol

Succinic acid

Conclusions Biorefinery on protein-rich cakes

Extraction processes Chemical Enzymatic

Use of proteins/peptides requires mild oil extraction

Production of biobased chemicals is important

Research necessary Obtaining amino acids Fractionation of amino acids

Remarks/questions

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