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Extraction of Peptides and
Amino Acids
Wim MulderSustoil, Foggia 24 April 2009
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Composition oil cakes
Table 1. Composition of rapeseed, sunflower and olive pulp
Rapeseed Sunflower Olive pulp Water 5.0 6.7 50.0 Oil 41.6 53.3 22.0 Protein 26.2 22.9 1.6 Carbohydrates + fibres 23.4 14.0 24.9 Ash 3.8 3.1 1.5
Extraction of peptides and amino acids relevant for rapeseed and sunflower cake
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Amino Acids
Nutritional value Taste Physiological/chemical characteristics
Production Chemical Enzymatic Fermentation Extraction
Human food
Animal feed
Oil cakes Hydrolysis
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Protein hydrolysis
Complete hydrolysis 6 n HCl, 110°C, 24 hours 5 n NaOH, 100°C, 18 hours Salts incorporated Destruction amino acids
Enzymatic hydrolysis Peptides Immobilised enzymes
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Extraction before oil recovery
rapeseed or sunflower seed
rest seedspeptides and amino acids
aqueous hydrolysis
oil
pressing
fractionated amino acids
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Extraction after oil recovery
rapeseed or sunflower press cake
soluble proteinspeptides and amino
acids
acidic/alkaline extractionenzymatic hydrolysis
peptides and amino acids
aqueous hydrolysisfractionated amino acids
fractionated amino acids
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Simultaneous extractionground oilseed
alkaline extraction centrifugation
solids: protein concentrateliquid
oil emulsion
acidic extraction centrifugation
3-phase centrifugation
liquid solids
3-phase centrifugation
oil
oil emulsion liquid
oil protein isolate
precipitation
ground oilseed
alkaline extraction centrifugation
solids: protein concentrateliquid
oil emulsion
acidic extraction centrifugation
3-phase centrifugation
liquid solids
3-phase centrifugation
oil
oil emulsion liquid
oil protein isolate
precipitation
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Chemicals from amino acids
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Biobased route for butanediol
Biomass
Proteins
Sugars
Amino acids
Other/rest amino acids
for food, feed or
chemicals
1,4-buanediol
Succinic acid
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Conclusions Biorefinery on protein-rich cakes
Extraction processes Chemical Enzymatic
Use of proteins/peptides requires mild oil extraction
Production of biobased chemicals is important
Research necessary Obtaining amino acids Fractionation of amino acids
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Remarks/questions
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