Predictors

Experimentalists

Assessors

Organizers

Center for CASP

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GDT_

TS

Cumulative percentage of targets

g427 results on all CASP14 targets

Accuracy of models in CASP14

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1

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50556065707580859095100CA

_RM

SDGDT_TS

Excited experimentalists session(or are protein models useful?)

Talks

1. Henning Tidow Professor, University of Hamburg2. Petr Leiman Associate Professor, University of Texas (UTMB)3. Osnat Herzberg Professor, University of Maryland4. Andrei Lupas Professor, Max Planck Institute, Tübingen

Discussion: brief communications

1. Steven Rees Post-doctoral fellow at UCSD2. Rhys Grinter Research Fellow at Monash Unversity3. Valerio Chiarini Post-doctoral fellow at University of Helsinki

Excited experimentalists session

T1058Integral membrane protein

(X-ray phasing)

Henning TidowUniversity of Hamburg

The model you sent me (from the leading group) worked for MR and we finally solved the structure by MR-SAD. I am still astonished that the human expert model worked, while none of the server models we tried did not (as they were rather similar). Great job!

Henning

Excited experimentalists session

H1097 [T1092, T1093, T1094, T1095, T1096]AR9 RNA Polymerase

(threading poly-A model into cryo-EM map)

Petr LeimanUniversity of Texas (UTMB)

Both my student (Alec Fraser in CC) and I are shocked… stunned… by the quality of the model… We need to re-evaluate what we do here. Alec remarked that he has just learned cryoEM and it is already an antiquated field. My conclusion - you do not need a $10M microscope to get an accurate model. You need to know people who can model your protein for you. Or you learn how do modeling yourself. Seriously, this is mind boggling. You would not believe how much effort we have put into getting this structure. Years of work… Both, cryoEM and crystallography… I mean, this is really shocking...

Petr

Excited experimentalists session

T1070 attachment region to the phage tail

(identify registry error)

Osnat HerzbergUniversity of Maryland

Unbelievable. They have predicted residues 16-75 correctly with an RMS of 1.26 A. Also, the prediction includes a different assignment of a cis proline (P236) than my original assignment. It turned out that the predicted version is correct because it enables repositioning of a tyrosine residue (Y247) in the right place. The change, together with another adjustment ultimately results in a 2-residue shift of 20 residues (237-256).

Osnat

Excited experimentalists session

T1100Transmembrane receptor

(NMR model could not help to solve the same protein)

Andrei Lupas, Max Planck Institute, Tübingen

I cannot overstate my excitement at the fact that Marcus solved the structure of Af1503 by molecular replacement with the models of group g427 and sent around the coordinates today. I will let him gloat over his success and share the structure when he is ready.

Andrei