epistatic gene interaction by biotechnology ciit abbottabad
DESCRIPTION
all types of gene action complementry epistaticTRANSCRIPT
……. Assignment no 1
ARRANGED BY; ; ; ZOHAIB HUSSAIN AND SAAD IQBAL
TOPIC ; ; ; ; EPISTATIC INTERACTIONS
SUBMITTED TO:::;;;;;DR SARFARZ SHAFIQ
COURSE TITLE ; ; ; ; ; ;ESSENTIAL OF GENETICS
•Epistatic Gene Interactions
Epistatic Gene Interactions
• Gene interactions occur when two or more different genes influence the outcome of a single trait
• Most morphological traits (height, weight, color) are affected by multiple genes
• Epistasis describes situation between various alleles of two genes
• Quantitative loci is a term to describe those loci controlling quantitatively measurable traits
• Pleiotropy describes situations where one gene affects multiple traits
• examine cases involving 2 loci (genes) that each have 2 alleles
• Crosses performed can be illustrated in general by– AaBb X AaBb
– Where A is dominant to a and B is dominant to b• If these two genes govern two different traits
– A 9:3:3:1 ratio is predicted among the offspring– simple Mendelian dihybrid inheritance pattern
• If these two genes do affect the same trait the 9:3:3:1 ratio may be altered– 9:3:4, or 9:7, or 9:6:1, or 8:6:2 or 12:3:1, or 13:3, or 15:1– epistatic ratios
A Cross Producing a 9:7 ratioFigure 4.18
9 C_P_ : 3 C_pp :3 ccP_ : 1 ccpp
purple white
Epistatic Gene Interaction• Complementary gene
actionEnzyme C and enzyme P
cooperate to make a product, therefore they complement one another
Epistatic Gene Interaction• Epistasis describes the situation in which
a gene masks the phenotypic effects of another gene
• Epistasis interactions arise because the two genes encode proteins that participate in sequence in a biochemical pathway
• If either loci is homozygous for a null mutation, none of that enzyme will be made and the pathway is blocked
Colorless precursor
Colorless intermediate
Purple pigment
Enzyme C Enzyme P
genotype cc
Colorless precursor
Colorless intermediate
Purple pigment
Enzyme C Enzyme P
Epistasis of Involving Sex-linked Genes
• Inheritance of the Cream-Eye allele in Drosophila– a rare fly with cream-colored eyes identified
in a true-breeding culture of flies with eosin eyes
– possible explanations• 1. Mutation of the eosin allele into a
cream allele• 2. Mutation of a 2nd gene that modifies
expression of the eosin allele
–Cream-colored eyes in fruit flies are due to the effect of a second gene that modifies the expression of the eosin allele
Testing the Hypothesis
Data obtained Cross OutcomeP cross:
Cream-eyed male X
wild-type female
F1: all red eyes
F1 cross:
F1 brother X F1 sister
F2: 104 females with red eyes
47 males with red eyes
44 males with eosin eyes
14 males with cream eyes F2 generation contains males with eosin eyes
This indicates that the cream allele is not in the same gene as the eosin allele
Interpreting the Data
Cross OutcomeP cross:
Cream-eyed male X
wild-type female
F1: all red eyes
F1 cross:
F1 brother X F1 sister
F2: 104 females with red eyes
47 males with red eyes
44 males with eosin eyes
14 males with cream eyes F2 generation contains –
151 + eye: 44 we eye: 14 ca eyea 12 : 3 : 1 ratio
Modeling the Data
Cream phenotype is recessive therefore the cream allele is recessive allele (either sex-linked or autosomal)
The mutated allele of the cream gene modifies the we allele, while the wt cream allele does not C = Normal allele
Does not modify the eosin phenotype ca = Cream allele
Modifies the eosin color to cream, does not effect wt or white allele of white gene.
Male gametes
CY
CCXw+Xw+ CCXw+Y cacaXw+Xw+ CcaXw+YCXw+
CXw+ caXw+ caY
CXw-e
caXw+
caXw-e
CCXw+Xw-e CCXw-eY CcaXw+Xw-e CcaXw-eY
CcaXw+Xw+ CcaXw+Y cacaXw+Xw+ cacaXw+Y
CcaXw+Xw-e CcaXw-eY cacaXw+Xw-e cacaXw-eY
Fem
ale
gam
etes
A Cross Involving a Two-Gene Interaction Can Still Produce a 9:3:3:1 ratio
• Inheritance of comb morphology in chicken– First example of gene interaction– William Bateson and Reginald Punnett in 1906– Four different comb morphologies
N
Figure 4.17b
The crosses of Bateson and Punnett
• F2 generation consisted of chickens with four types of combs
– 9 walnut : 3 rose : 3 pea : 1 single
• Bateson and Punnett reasoned that comb morphology is determined by two different genes– R (rose comb) is dominant to r– P (pea comb) is dominant to p – R and P are codominant (walnut comb)– rrpp produces single comb
Gene Interaction• Duplicate gene action
– Enzyme 1 and enzyme 2 are redundant
– They both make product C, therefore they duplicate each other
Duplicate Gene Action Epistasis
TV
TV
Tv
Tv
tV
tV
tv
tv
TTVV TTVv TtVV TtVv
TTVv TTvv TtVv Ttvv
TtVV TtVv ttVV ttVv
TtVv Ttvv ttVv ttvv
(b) The crosses of Shull
TTVVTriangular
ttvvOvate
TtVvAll triangular
F1 (TtVv) x F1 (TtVv)
x
F1 generation
15:1 ratio results
Bombay Phenotype
Bombay Phenotype
Bombay Phenotype
Squash Fruit Color
• Genotypes and Phenotypes:
• W-/G- white
• W-/gg white
• ww/G- green
• ww/gg yellow
Dominant Epistasis
• LETS HAVE A LOOK AT DOMINANT EPISTASIS…
• Squash fruit color is controlled by two genes.
• Gene 1 is represented by a W• Gene 2 is represented by a G
Squash Fruit Color
• Which allele is epistatic in squash color?
• How do you know?The dominant W allele is epistasis
Because every time a dominant W allele shows up in a squash genotype, the squash fruit color is white.
Wwgg x wwGg
• FOIL: Wg or wg• FOIL: wG or wg
• F1 generation genotypes:
• Phenotypes:
....THANK.
. ……YOU…..
REFRENCE BOOKS
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