enzyme structure · 13. application of transient electric birefringence to paul j. hagerman 198 the...
TRANSCRIPT
Methods in Enzymology
Volume 11 7
Enzyme StructurePart J
C. H . W . Hirs
Serge N. Timasheff
CONTRIBUTORS TO VOLUME 117 Vi i
PREFACE i X
VOLUMES IN SERIES X i
Section I . Size, Shape, and Polydispersity of Macromolecule s
1 . Characterization of Macromolecules by Sedimen- ROBERT J . POLLET
3
tation Equilibrium in the Air-Turbine Ultracen-
trifuge
2 . Measurement of Changes of Hydrodynamic Prop- ROBERT W . OBERFELDER ,
erties by Sedimentation
THOMAS G . CONSLER, AND
JAMES C . LEE
27
3 . Determination of Protein Molecular Weight in JACQUELINE A . REYNOLD S
Complexes with Detergent without Knowledge AND DARRELL R . MCCASLIN 4 1of Binding
4. Calculation of Partial Specific Volumes of Proteins V . PRAKASH AND
in 8 M Urea Solution
SERGE N . TIMASHEFF
5 3
5 . Calculation of the Partial Specific Volume of Pro- T . ARAKAWA AN D
teins in Concentrated Salt and Amino Acid So- SERGE N . TIMASHEFF
60
lution s
6 . Molecular Weight Determinations from Radiation J . T . HARMON ,
Inactivation
T . B . NIELSEN, AN D
E . S . KEMPNER
65
7. Measurement of Molecular Weights of Elongated BRUNO H . ZIMM
94
Molecules by Viscoelastometry
8. Measurement of Hydrodynamic Properties of Ac- LYNDAL K . HESTERBERG AN D
tive Enzyme by Sedimentation
JAMES C . LEE
9 7
9 . Computer-Controlled Scanning Gel Chromatog- LAWRENCE C . DAVIS ,
raphy
THEODORE J . SOCOLOFSKY ,
AND GARY A . RADKE
11 6
10. Hydrodynamic Characterization of Random Coil PHIL G . SQUIRE
142Polymers by Size Exclusion Chromatograph y
11 . Structural Interpretation of Hydrodynamic Mea- THOMAS F . KUMOSINSKI AN D
surements of Proteins in Solution through Cor- HELMUT PESSEN
15 4relations with X-Ray Data
12 . The Use of Covolume in the Estimation of Protein LAWRENCE W . NICHOL AND
Axial Ratios
DONALD J . WINZOR
18 2
13 . Application of Transient Electric Birefringence to PAUL J . HAGERMAN
198
the Study of Biopolymer Structure
14 . Measurements of Protein Hydration by Various HELMUT PESSEN AN D
Techniques
THOMAS F . KUMOSINSKI
21 9
15 . Correlation Function Profile Analysis of Polydis- B . CHU, J . R . FORD, AN D
perse Macromolecular Solutions and Colloidal H . S . DHADWAL
25 6
Suspension s
Section II . Interaction of Macromolecules with Ligands and Linkages
16. Nonlinear Least-Squares Analysis
MICHAEL L . JOHNSON AN D
SUSAN G . FRASIER
30 1
17 . Ligand Binding to Proteins by Equilibrium Gel HIROsHI KIDO ,
Penetration
ALBERTO VITA, AN D
B . L . HORECKER
342
18 . Measurement of Protein-Ligand Interactions by Jost MANUEL ANDREU
346
Gel Chromatograph y
19 . Ultrafiltration in Ligand-Binding Studies
ALKIS J . SOPHIANOPOULOS AND
JUDITH A . SOPHIANOPOULOS 35 4
20 . Protein Chromatography on Hydroxyapatite MARINA J . GORBUNOFF
370
Column s
21 . Measurement of Ligand-Protein Interaction by ROBERT W . OBERFELDER AN D
Electrophoretic and Spectroscopic Techniques JAMES C . LEE
38 1
22 . Measurement of Ligand Binding to Proteins by LARRY D . WARD
400
Fluorescence Spectroscopy
23 . Measurement of Metal-Ligand Distances by ROBERT A . SCOTT
41 4
EXAF S
24 . Velocity Sedimentation Study of Ligand-Induced GEORGE C . NA AN D
Protein Self Association
SERGE N . TIMASHEFF
459
25 . Measurement and Analysis of Ligand-Binding Iso- GEORGE C . NA AN D
therms Linked to Protein Self-Associations
SERGE N . TIMASHEFF
496
AUTHOR INDEX 52 1
SUBJECT INDEX 535