Environmental Solutions

from

Environmental Solutions from Dionex

22427

SamplesDetection

Data Analysis and Reporting

Extraction Separation

ASE®—Accelerated Solvent Extraction

Choose the ASE that Matches Your Laboratory Productivity Requirements

U.S. EPA SW-846 Method 3545 (A)– Pesticides and herbicides– Semivolatiles and PAHs– PCBs– PCDDs and PCDFs– TPH (DRO)– Explosives

Air samples– PUF filters– Quartz filters– XAD resins– Tenax resins– Charcoal adsorbents

Organotin and organoarsenic compounds

BFRs Ionic materials

Automated Sample Concentration

ASE® with In-Cell Cleanup (ICC) Saves Time and LaborBrominated Flame Retardants in Fish Tissue

Extraction and Sample Prep in One Easy Step

Extracts With and Without In-Cell Cleanup of Fish Tissue Using Alumina, Silica Gel, and Acidic Silica Gel (40% H2SO4)

In-Line Sample PrepWithout ICC

With ASE-ICC

Calibration Check

Salmon, ASE-ICC

In-Line Cleanup in ASE® CellExtraction and Sample Prep in One Easy Step

Automated Perchlorate Sample Preparation

0 5 10 15 20–500

0

3,500 1. Alfalfa—Extraction Only2. Alfalfa—Extraction + Sample Prep

µS

Minutes

12

Sample and Dispersant

Alumina Sorbent and OnGuard® Resin Mixture

Extraction FlowNew

Method

Columns for Environmental AnalysisAcclaim® Columns for

Reversed Phase Separations

IonPac® Columns for Ion Exchange Separations

22427

Typical Anions Found in Drinking Water New AS22 Column

Unique COLUMN

CHEMISTRY

Conceptual Illustration of Surface Modification

0 5 10 13–1.00

7.00

µS

Minutes

1

2

3

45

6

7

8

Peaks: ppm1. Fluoride 5 2. Acetate 203. Chloride 104. Nitrite 155. Bromide 256. Nitrate 257. Phosphate 408. Sulfate 30

AS22 Column Method 300.0 Inorganic Anions Drinking Water

Baseline Resolution with High Salt Samples

0 5 10 14–0.200

0.500 1

2 4

56

7

8

9 10

11

µS

3

0 5 10 14–5.0

45.0

12

3

456 7

8 9 1011

Minutes

µS

Peak ppm1. Fluoride 0.842. Formate 0.033. Chloride 15.64. Nitrite 0.015. Unknown NQ6. Chlorate 0.187. Bromide 0.028. Nitrate 0.899. Carbonate NQ

10. Phosphate 0.2211. Sulfate 20.45

Cations in Drinking Water Sample ASTM Method D6919-03*

48 mM Methanesulfonic Acid

Minutes0 2 4 6 8 10 12

0.10

1.00

1 3µS

2 54 6

30 mM Methanesulfonic Acid

0 5 10 15 20 250.10

1.00

1 3

4

µS2 5 6

*EPA proposed for drinking water and wastewater compliance monitoring

Peaks 1. Lithium 0.002 ppm 2. Sodium 19.73. Ammonium 0.074. Potassium 0.995. Magnesium 7.26. Calcium 18.5

RFIC:Easy method developmentImmediate results

Acclaim Explosives ColumnsA Total Solution for SW-846 Method 8330

1. HMX2. RDX3. 1,3,5-Trinitrobenzene4. 1,3-Dinitrobenzene 5. Nitrobenzene6. Tetryl7. 2,4,6-Trinitrotoluene8. 4-Amino-2,6-dinitrotoluene9. 2-Amino-4,6-dinitrotoluene

10. 2,6-Dinitrotoluene11. 2,4-Dinitrotoluene12. 2-Nitrotoluene13. 4-Nitrotoluene14. 3-Nitrotoluene

12

14

3 4

10 119

12 13

5 7 86

Acclaim Explosives E1

AU

0 18 36279 45Minutes

1 214

3 4

1011

91213

5 7

86

Acclaim Explosives E2

Baseline Resolution!

Haloacetic Acids by IC-MS/MSMethod Being Jointly Development by EPA and Dionex

No Derivatization—No Sample Prep

Peaks

1. Chloroacetic acid` 6 ppm

2. Bromoacetic acid 4 3. Dichloroacetic acid 6 4. Bromochloroacetic acid 4 5. Dibromoacetic acid 2 6. Bromide 2 7. Trichloroacetic acid 2 8. Bromodichloroacetic acid 2 9. Chlorodibromoacetic acid 10

10. Tribromoacetic acid 20Minutes

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 380

4800

Inte

nsity

, cps

1 67

8

9

103

4

5

Direct Injection

2New

Method

Novel LC Separation of Nitrosamines for EPA 8270 Using Acclaim® PA

81,200,000

2.2 11.40

1 2 3

4

5 6 79

ESI Overlay

Minutes

Inte

nsity

0 30

0

220 1

2 3

4 567

89mAu

Accurate Analysis of Thermally Labile Compounds!!

20109a

1. N-nitroso-dimethylamine2. N-nitroso-morpholine 3. N-nitroso-methylethylamine

4. N-nitroso-pyrrolidine 5. N-nitroso-diethylamine 6. N-nitroso-piperidine 7. N-nitroso-dipropylamine 8. N-nitroso-dibutylamine9. N-nitroso-diphenylamine

Determination of Carbonyl-DNPH Derivatives Using Coupled Acclaim® PA and Acclaim® C18 Columns

0 4 8 12 16 20 24 28 32 35

1

2

34

567

8

910

11 1213mAU

Minutes

Couple Two Columns: More platesCouple Different Columns: Improved selectivity

DNPH derivatives of:1. Formaldehyde2. Acetaldehyde3. Acrolein4. Acetone5. Propionaldehyde 6. Crotonaldehyde7. Methacrolein8. Butyraldehyde9. Methyl ethyl ketone

10. Benzaldehyde11. Valeraldehyde12. m-Tolualdehyde13. Hexaldehyde

20112

Acclaim® Surfactant Column Separatesa Wide Variety of Surfactants!

Peaks:1. Chloride2. Bromide3. Nitrate4. Xylene sulfonate5. Laurylpyridinium chloride6. Lauryldimethylbenzyl-NH4Cl7. Triton X-1008. Cetyl betaine9. Decyl sulfate

10. Dodecyl sulfate11. C10–LAS12. C11–LAS13. C12–LAS14. C13–LAS

mV

0 5 10 15 20 25 30 35Minutes

1

23

4

56

10

98

1112

13

14

Awards Winning Systems from Dionex!

ICS-3000

Flexible, dual RFIC™ system

Proportioned and RFIC™

gradient system

Multiple detectors

System controller

Multiple thermal zones

Integrated Automation

Dionex Ion Chromatography Systems

A Family of IC and RFIC™ Systems

ICS-1600

Standard integrated designElectrolytic suppressionLCD front panelColumn heater

ICS-900

Entry-level integrated design

Chemical suppression

ICS-1100

Basic integrated design

Electrolytic suppression

ICS-2100

Dedicated, single RFIC™ systemRFIC™ gradient systemElectrolytic suppressionLCD front panelColumn Heater

Innovative Eluent Generation Technology

Vent

Pump

H2O

[KOH] Current

Flow Rate

K+

ElectrolyteReservoir

K+

KOH

O2

DegasUnit

KOHGeneration

Chamber

Cation Exchange Connector

CR-CTC Cation Trap

Pt Anode

(H2O 2H+ + ½O2 + 2e–)

[ + ]

Pt Cathode(2H2O + 2e– 2OH– + HO2 )

[ – ]

KOH + H2

EluGen® KOH Cartridge

14538-06

InnovativeTechnology

RFIC® Systems Let You Take Advantage of Hydroxide Eluents

0.00.20.40.60.81.01.21.41.61.82.02.2

300.1 317 326 300.1 317 326

Con

cent

ratio

n (µ

g/L)

AS19 (Hydroxide Eluent) AS9-HC (Carbonate Eluent)

ChloriteBromateBromate-PCRChlorateBromide

Lower MDLs Using Hydroxide Eluent!

Carbonate Removal Device (CRD)

Innovation:– Permeable membrane device

reduces carbonate– No reagents required– No mechanical pumps required

Values:– Minimizes interference from

carbon dioxide in samples– Improves sensitivity for trace analysis– Improves peak integration

and quantitation of anionsImproved Sensitivity for Trace Anion Analyses

EluentIn

Sample Volume

CO2 (gas)CO2 (gas)Eluent

Out

Regen In(Base from Suppressor) CO3

2–

(Carbonate)

CO32–

(Carbonate)

Regen OutRegen In

To the Conductivity Cell

Inlet Port

Regen Out(To CR-TC)

From Eluent Out Port of the Suppressor

22280

InnovativeTechnology

Low-Level Perchlorate in Drinking WaterWith and Without Carbonate Removal Device

22685

Column: IonPac® AG20, AS20 2 mmEluent: 0.5 mM NaOH 0–12 min

65 mM 12.1–28 mm, 100 mM28.1–35 min

Eluent Source: EGC NaOH with CR-ATCTemperature: 35 °CFlow Rate: 0.25 mL/minInj. Volume: 2 mLRinse Volume: 1 mL (10 mM NaOH)Concentration: IonPac Cryptand C1 (4 × 35 mm)Detection: ASRS® ULTRA II, 2 mm

AutoSuppression external water mode

Peaks: 1. Perchlorate 0.5 µg/L (ppb)2. Unknown —

Concentration: 0.50 mM

µS

0 5 10 20 3515 25 30

1

0

Minutes

65.00

100.00

A: With CRD

B: Without CRD

1

1

2

2

Improved Sensitivity for Trace Perchlorate Analyses

Method 314.1

5 7.5 100

18,000

0

4

SIM 99Counts

µS

Minutes

Conductivity

SIM 99

SIM 101

Perchlorate EPA Method 332.0 (IC-MS)*

Column: IonPac® AG16, AS16, 2-mm i.d.Suppressor: ASRS® ULTRA, 2 mmEluent: 65 mM KOH (EG40)Flow Rate: 0.30 mL/minInj. Volume: 250 µLDetection: 1. Conductivity

2. MSQ, SIM 99, 35CIO4–

3. MSQ, SIM 101, 37CIO4–

MS Conditions: –ESI, 70 V, 350 °CSample: Groundwater diluted 1/10

Peak: Perchlorate ~ 7–8 μg/L

* Method Developed Jointly by EPA-OGWDW and Dionex

Method 332.0

EPA Perchlorate Method 332.0*IC-MS/MS

* Method Developed Jointly by EPA-OGWDW and Dionex

Minutes

Peak Area = 1.00 x e4 Counts

9830

0

Coun

ts/s

ec

0

150

Coun

ts/s

ec

0

485

Coun

ts/s

ec

Peak Area = 2.04 x e5 Counts

Peak Area = 3.16 x e3 Counts

2 4 6 8 10 12 14

Internal StandardMRM 107.0/89.035Cl18O3

1 µg/L (ppb)

MRM 100.9/84.837Cl16O3

5 ng/L (ppt)

MRM 98.9/82.835Cl16O3

5 ng/L (ppt)

16

IC System: Dionex ICS 2500 with Matrix Diversion and Auxiliary Pump

MS/MS System:MDS Sciex API 2000

Internal Standard:0–18 Perchlorate: 1 µg/L*

Inject Volume:100 µL

Perchlorate Isotope Ratio:10,000/3160 = 3.16

* Prepared from 1 mg/L stock standard (Dionex Corp)

Method 332.0

EPA Method 331.0* LC-MS/MS Perchlorate Analysis Using Ion-Exchange Separation,

Matrix Diversion, and MS/MS DetectionConceptual Illustration of Surface Modification

IonPac® AS21

LC-MS/MS Eluent: 200 mM methyl amineFlow Rate: 0.35 mL/minAnalysis Column:IonPac® AS21, 2 mmDiversion: Eluent divert valveDetector: Triple quadrupole MS/MS

* Method developed jointly by EPA-OGWDW and Dionex

Method 331.0

EPA Method 300.1 (B) for Bromate in Drinking Water

(A) (B)ppm ppm

1. Fluoride 0.092. Formate — 3. Chlorite — 0.0204. Bromate — 0.0055. Chloride 72.7 6. Nitrite 0.0907. Chlorate 0.120 0.270 8. Bromide 0.200 0.4009. Nitrate 3.7

10. Carbonate — 11. Sulfate 58.4

–0.05

0.75

µS

7

8(A) Unspiked

1

2

5

6

9 10, 11

0 5 10 15 20 25 30–0.05

0.75

µS

8

Minutes

7

43

(B) Spiked

1

2

5

6

9 10, 11

New AS19 Column

RFIC:Lower

background Lower MDL

4

2-D IC Is Easy with the ICS-3000 System

DC Module

Eluent Generator

Auto Sampler

Dual PumpAutomationManager

Automated with Chromeleon Software

2-D IC Removes Interfering MatrixAS20 and AS16 Chemistry Combination

A. First DimensionColumn: IonPac® AG20, AS20, 4 mm Matrix: ppm Chloride: 1000Bicarbonate: 1000Sulfate: 1000

B. Second Dimension Column: IonPac AG16, AS16, 2 mmPeak: 1. Perchlorate 0.5 ug/L

0.75

1.35

µS

A. High Salt Matrix

0 10 20 30 400.40

1.00

1

µS

Minutes

B. Resolved Peak

Second DimensionResolves peakEnhances signal

Introducing UltiMate 3000 Systems Standard, Micro, and Semiprep LC

A New Family of Environmental HPLC Systems

New LC Systems

The UltiMate x2 Dual-Gradient LC System

Solvent Rack with 6-Channel Degasser

Unique Dual-Gradient Pump: Two Ternary Pumps in

One Housing Autosampler with Optional Thermostatting

Column Compartment with Up to Two Switching Valves

Variable Wavelength or Photodiode Array Detector

The Ultimate for Environmental Analysis Productivity

New LC Systems

Wavelength Scanning Allows Detection of Different Compounds from One Injection

Peaks: 1. Uracil 0.6752. Naphthalene 1.617

3. Biphenyl 1.8754. Fluorene 2.0925. Anthracene 2.4256. Fluoroanthene 2.792

210 nm

275 nm

0 0.50 1 1.50 2 2.50 3 3.50 4–500

0

3,000

mAU

Minutes

4321

1

2

3

45

6

286 nm

250 nm

Scan WavelengthsImprove

Identification!

Fast LC EPA 604 for Phenols

Peaks 1. Phenol2. 2,4- di-NO2-phenol3. 4- NO2-phenol 4. 2-Cl-phenol5. 2-NO2-phenol6. 2-Me-4,6-diNO2-phenol7. 2,4- di-Me-phenol8. 4-Cl-3-Me-phenol9. 2,4- di-Cl-phenol

10. 2,4,6- tri-Cl-phenol11. Penta-Cl-phenol

0 5 10 15–5

192

mAU

Minutes

3X faster with new Acclaim® Fast LC Columns