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LAB#01

1.1. STATEMENT:

LAYOUT OF ENVIRONMENTAL GEOLOGY LAB

1.2. SCOPE:

- To understand the lab equipment names and their functions

- To understand the position of equipment in the lab

1.3. THEORY:

1.3.1 ENVIRONMENTAL GEOLOGY:

Environmental geology is an applied science concerned with the practical application of the

principles of geology in the solving of environmental problems. This field involves the study of

the interaction of humans with the geologic environment. Environmental geology is the

application of geological information to solve conflicts, minimizing possible adverse

environmental degradation or maximizing possible advantageous condition resulting from the

use of natural and modified environment.

IMPORTANCE OF ENVIRONMENTAL GEOLOGY:

Environmental Geology is an important field because it is used for:

- Managing geological and hydrogeological resources such as fossil fuel, minerals, waters

(surface and subsurface) and land use.

- Defining and mitigating exposure of natural hazards on humans.

- Managing industrial and domestic waste disposal and minimizing or eliminating effects

of pollution.

- Studying the earth's surface through the disciplines of geomorphology.

- Performing associated activities, often involving litigation.

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Figure 1.1 Environmental interaction

Figure 1.2 Lab Layout

1.3.2. LAB LAYOUT:

1.4.LIST OF INSTRUMENTS:

1) Autoclave incubator

2) Oven

3) Paqualab

4) Electrical conductance meter (EC meter)

5) Sensodirect

6) PH meter

7) Paqualab photometer

8) Turbidity meter

9) Hot plate

10) Multi gas detector

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Figure 1.3 Autoclave

1.5.DETAILS OF INSTRUMENTS:

1.5.1. AUTOCLAVE INCUBATOR:

An autoclave is an instrument used to sterilize equipment and supplies by subjecting them to

high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the

load and the contents. Many autoclaves vary in size and function depending on the media they

are sterilizing.

USES OF AUTOCLAVE:

- Autoclaves are widely used in microbiology, medicine, body piercing, veterinary science,

dentistry, research and development for pharmaceutical and food production facilities and

prosthetic fabrication.

- These are the sterilization equipment of choice in laboratories and hospitals.

- Large autoclaves and units of smaller sizes may be used anywhere else that sterilization of

equipment is critical to ensuring the outcome of the process, the safety of personnel or the

public, such as in businesses which provide tattooing and body piercing services.

1.5.2.OVEN: An oven is a thermally insulated chamber used for the heating, baking or drying of a substance

and most commonly used for cooking. Different types of ovens are used for different purposes

but we use oven in our lab mostly for drying purpose.

USES OF OVEN:

- An autoclave is an oven-like device with features similar to a pressure cooker that allows the

heating of aqueous solutions to higher temperatures than water's boiling point in order to

sterilize the contents of the autoclave.

- A kiln is a high-temperature oven used in wood drying, ceramics and cement manufacturing

to convert mineral feedstock.

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Figure 1.4 Oven

Figure 1.5 Paqualab

1.5.3. PAQUALAB:

An incubator provides a controlled environment that regulates temperature, ventilation and

humility. It is used to care for premature babies, hatch poultry eggs and to cultivate

microorganisms. A laboratory incubator is also used for tissue culture, a type of clinical research

method that draws out tissue parts from animals or plants. A laboratory incubator is used in

genetic engineering, which is an extended application of tissue culture.

USES OF PAQUALAB:

- It is used for the culturing the, microorganisms environment.

- It is used for keeping premature in fact in the temperature same to that of their mother’s

womb.

Used for hatching eggs. An incubator is a device used to grow and maintain microbiological

cultures or cultures.

- Hospitals will always rely on neonatal incubators while the field of research and bio-

technology will mainly depend on laboratory incubators.

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Figure 1.6 EC Meter

Figure 1.7 Senso Direct

1.5.4. ELECTRICAL CONDUCTIVITY METER:

An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. It

is commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of

nutrients, salts or impurities in the water.

USES OF EC METER:

- The electrical conductivity of water estimates the total amount of solids dissolved in water,

which stands for Total Dissolved Solids.

- The electrical conductivity of water is actually a measure of salinity. Excessively high

salinity can affect plants in the following ways:

1. Specific toxicity of a particular ion (such as Sodium)

2. Higher osmotic pressure around the roots prevents an efficient water absorption by the

plant.

1.5.5. SENSODIRECT:

The SensoDirect is designed for multipurpose operation and measures PH/Redox, dissolved

oxygen and conductivity/TDS. It incorporates an intuitive user interface, large, easy to read

display and is supplied with a sturdy handy case with electrodes, buffer solution and accessories.

USES OF SENSODIRECT:

It is used to measure:

- PH/Redox

- Dissolved oxygen and conductivity/TDS

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Figure 1.8 PH Meter

Figure 1.9 Photometer

1.5.6.PH METER:

A PH meter is an electronic instrument used for measuring the pH (acidity or alkalinity) of a

liquid (though special probes are sometimes used to measure the pH of semi-solid substances.

The pH of a river or lake is important in maintaining a proper ecological balance.

USES OF PH METER:

- Used in hospitals, universities Pharma companies, I.I.T, research institutes or laboratories.

- It is also used in the wine industry and in the process of the fermentation In Food Science in

the Pulp and Paper Industry in Chemical Research and Engineering.

1.5.7.PAQUALAB PHOTOMETER:

A photometer is an instrument for measuring light intensity or optical properties of solutions or

surfaces.

USES OF PHOTOMETER:

- Photometers are used to measure the different properties i.e., Illuminance, Irradiance,

absorption, Scattering, Reflection, Fluorescence, Phosphorescence, Luminescence.

- Whereas the microscope photometer can be used to measure the Vitrinite Coal

Reflectometry, Kerogen analysis, Mineralogy.

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Figure 1.10 Turbidty Meter

Figure 1.11 Hot plate

1.5.8.TURBIDITY METER:

A Turbidity Meter is a stationary or portable instrument for measuring suspended particulates in

a liquid or gas colloid. The measurement of turbidity is a key test of water quality.

USES OF TURBIDITY METER:

- The turbidity meter is extensively used in the testing of domestic water supplies as these have

to be safe for human consumption.

- It is also used in the treatment process to access how hard filters will have to work to treat

rainwater from rivers. The process is useful in determining the quality and state of water.

- In rivers these meters are often used to find sediment and silt levels of the flowing water.

1.5.9.HOT PLATE:

A hot plate is a portable self-contained tabletop small appliance that features one, two or more

gas burners or electric heating elements. It is essentially an electric stove top that is used in the

laboratory.

USES OF HOT PLATE:

- A hot plate can be used as a standalone appliance, but is often used as a substitute for one of

the burners from an oven range or the cook top of a stove.

- This laboratory hot plate with magnetic stirrer is used for preparing chemicals used in

research. In laboratory settings, hot plates are generally used to heat glassware or its contents.

A hot plate is an adjustable heating source which is ideal for heating beakers, flasks, hot

water baths, and other flat-bottomed containers.

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1.5.10.MULTI GAS DETECTOR:

Multi-gas monitor capable of detecting O2, CO, H2S and combustible gases for a wide variety of

hazardous and confined space applications. It can accommodate either NiMH or alkaline

batteries, which are interchangeable.

USES OF MULTIGAS DETECTOR:

- Detects O2, H2S, CO and combustible gas and is used for confined space entry in industrial,

agricultural and other environments as

well as by fire and rescue personnel.

1.6.REFERENCES:

http://www.lovibond.com/en/pool/turbidity-meters

http://www.lovibond.com/en/pool/photometer

http://en.wikipedia.org/wiki/Oven

http://en.wikipedia.org/wiki/Electrical_conductivity_meter

http://en.wikipedia.org/wiki/Environmental_geology

Figure 1.12 Multi Gas Detector

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Figure 2.1 Autoclave

Figure 2.2 Autoclave

LAB#02

2.1. STATEMENT:

STERLIZATION OF LAB EQUIPMENT BY USING AUTOCLAVE

2.2. SCOPE:

- It is used for the treatment of biological waste

- Safety is provided by using autoclave

2.3. THEORY:

2.3.1. AUTOCLAVE:

An autoclave is an instrument used to sterilize equipment and supplies by subjecting them to

high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the

load and the contents. Many autoclaves vary in size and function depending on the media they

are sterilizing.

USES OF AUTOCLAVE:

- Autoclaves are widely used in microbiology, medicine, tattooing, body piercing, veterinary

science, dentistry, chiropody and prosthetic fabrication.

- Typical sterilization includes laboratory glassware, surgical instruments, medical waste and

patient care utensils.

- A notable growing application of autoclaves is in the pre-disposal treatment and sterilization

of waste material, such as pathogenic hospital waste.

- Autoclaves are also widely used to cure composites and in the vulcanization of rubber.

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Figure 2.3 Structure of Autoclave

2.4. STERILIZATION:

Sterilization is a term referring to any process that eliminates (removes) or kills all forms of

microbial life, including transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.)

present on a surface, contained in a fluid, in medication, or in a compound such as biological

culture media. Sterilization can be achieved by applying the proper combinations of heat,

chemicals, irradiation, high pressure, and filtration.

2.4.1. IMPORTANCE OF STERILIZATION:

Sterilization is very important process because it has wide uses and applications, some of the

Applications of sterilization are given as:

- Medicine and surgery

- Food

- Industry purposes

2.4.2. TYPES OF STERILIZATION:

HEAT STERILIZATION:

Heat Sterilization can be:

1) DRY HEAT STERILIZATION

Dry heat sterilization of an article is one of the earliest forms of sterilization practiced. Dry heat,

as the name indicates, utilizes hot air that is either free from water vapour, or has very little of it,

and where this moisture plays a minimal or no role in the process of sterilization.

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2) MOIST HEAT STERILIZATION:

Heating an article is one of the earliest forms of sterilization practiced. Moist heat, as the name

indicates, utilizes hot air that is heavily laden with water vapour and where this moisture plays

the most important role in the process of sterilization.

STEAM STERILIZATION:

A widely used method for heat sterilization is the autoclave, sometimes called a converter.

Autoclaves use steam heated to 121–134 °C (250–273 °F) under pressure (100 °C (212 °F) is the

maximum attainable at atmospheric pressure). To achieve sterility, a holding time of at least 15

minutes at 121 °C (250 °F) at 100 kPa (15 psi), or 3 minutes at 134 °C (273 °F) at 100 kPa (15

psi) is require. This is the time for which all the material being sterilised must be held at the

specified temperature; additional time is required to heat the material, unless finely ground.

2.5. PROCEDURE OF STERILIZATION BY AUTOCLAVE:

Dip the element in the water

Put the equipment in the water in the bucket like assembly of the autoclave.

Operate the timer for the 30 minutes and adjust the temperature for the 1210 C.

After 30 minutes there will be buzzer from the apparatus which will be the sign of the

sterilization of the equipment.

2.5.1. ADVANTAGES:

- Is used for the killing of hazardous materials from the daily used equipment.

- Provide safety against diseases

- The process is not so difficult

- Can be used for the treatment of wastes which is a big problem to the environment

- Have large number of applications in most of the industries

2.5.2. DISADVANTAGES:

- Autoclave is a good method for sterilization but as we move the sterilized equipment from

the autoclave it have contact with the moisture, resulting the initiation of the culturing of the

bacteria.

- Steam sterilization has some deleterious effects on some materials, including corrosion and

combustion of lubricants associated with dental hand pieces.

- High temperatures are not suitable for most materials.

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2.6. PRECAUTIONS:

Instruments that have undergone sterilization can be maintained in such condition by

containment in sealed packaging until use.

2.7. REFERENCES:

- Article of Sidra Roa PN Assistant Professor Department of Microbiology JJMMC,

Davangere (Last edited on June 2008)

- http://en.wikipedia.org/wiki/Sterilization

- "No bugs please, this is a clean planet!". European Space Agency. 30 July 2002. Retrieved 7

August 2014

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Figure 3.2 Probe

LAB#03

3.1. STATEMENT:

DETERMINE THE PH AND TURBIDITY OF WATER SAMPLES BY USING PH

METER AND TURBIDITY METER

3.2. SCOPE:

- Good quality of water needs PH and Turbidity level according to standards.

- Using the TDS meter and the PH meter, determination of the quality of the water under

different physical parameters.

3.3. THEORY:

3.3.1. PH METER:

A PH meter is an electronic instrument used to measure the PH (acidity or alkalinity) of a liquid

(though special probes are sometimes used to measure the PH of semi-solid substances). A

typical PH meter consists of a special measuring probe (a glass electrode) connected to an

electronic meter that measures and displays the PH reading.

3.3.2. THE PROBE:

The PH probe measures PH as the activity of hydrogen ions surrounding a thin-walled glass bulb

at its tip. The probe produces a small voltage (about 0.06 volt per PH unit) that is measured and

displayed as PH units by the meter.

Fig. pH meter

Figure 3.1 PH Meter

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3.4. PROCEDURE TO DETERMINE PH:

First of all, start the PH meter.

Now calibrate the PH meter.

Take approximately 100 ml water sample in beaker of which PH has to measure.

Dip the probe in water sample.

PH meter display the reading continuously changing, when reading becomes constant, note

that reading.

- PH = 7 Neutral

- PH > 7 Basic

- PH < 7 Acidic

3.5. TURBIDITY:

The haziness of the water is known as the turbidity of the water. Turbidity is a measure of the

degree to which the water loses its transparency due to the presence of suspended particulates.

Turbidity is a principal physical characteristic of water and is an expression of the optical

property that causes light to be scattered and absorbed by particles and molecules rather than

transmitted in straight lines through a water sample. It is caused by suspended matter or

impurities that interfere with the clarity of the water. These impurities may include clay, silt,

finely divided inorganic and organic matter, soluble colored organic compounds, and plankton

and other microscopic organisms. Main causes of the turbidity are as under;

- Sediments from erosion

- Resuspended sediments from the bottom

- Waste discharge

- Algae growth

- Urban runoff

3.5.1. TURBIDITY METER:

Sensor uses light which passes in the water at right angle to detect the water's turbidity .Ensure

that the minimum amount of external light possible is exposed to the monitoring site. . The

turbidity meter will display readings directly in either nephelometric turbidity units (NTU) or

parts per million (PPM).For environmental or process monitoring, simply place the turbidity

sensor directly in the water and position it where the turbidity is to be monitored.

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Figure 3.3 Turbidity Meter

3.5.2. FEATURES OF TURBIDITY METER:

Turbidity Meter combines the turbidity sensor with a handheld meter that has a six digit LED

screen, 4-button control panel, and an internal 9V battery. The turbidity meter will display

readings directly in either nephelometric turbidity units (NTU) or parts per million (PPM). The

turbidity meter also includes an automatic shutoff feature to conserve battery power.

3.6. PROCEDURE OF DETERMINE TURBIDITY:

Shaking the sample bottle well before analysis.

The sample is simply poured into a glass tube.

Then place the glass tube inside the instrument.

The result is read directly from the instrument.

3.7. W.H.O STANDARDS:

The WHO establishes that pH of drinking water should be in between 6 to 8.5.

The WHO (World Health Organization), establishes that the turbidity of drinking water

shouldn't be more than 5 NTU.

TDS < 200 mg/liter (Not Good)

TDS 200-500 mg/liter (Fit)

TDS 500-800 mg/liter (Moderate)

TDS >800 mg/liter (Not Good)

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Figure 3.4 Locations of Samples

3.8. OBSERVATION AND CALCULATIONS:

TABLE#3.1

Sample

No.

Sample

location

Sample

ID

Smell/taste Color PH Potential

(mv)

Turbidity

(mg/lit)

Temp

(0C)

1 Cant C-1 Taste less Colorless 6.98 54.5 2.44 19.2

2 Cant C-2 Taste less Colorless 7.08 50.4 1.56 22.3

3 Cant C-3 Taste less Colorless 7.64 26.6 2.21 20.1

4 F.F.UET Uet-3 Taste less Colorless 7.09 150.1 4.92 19.4

5 UET

Q hall

Uet-4 Taste less Colorless 7.15 247.4 5.41 20.4

6 Cant C-4 Taste less Colorless 7.12 48.5 1.28 20.0

7 UET

L hall

Uet-1 Taste less Colorless 7.43 35.7 5.29 19.93

8 UET SMG

hall

Uet-2 Taste less Colorless 6.64 69.0 6.21 19.7

9 Shalimar

link road

SL-1 Taste less Colorless 723 44.0 1.61 21.5

10 Shahdra SHD-1 Taste less Colorless 7.07 50.7 1.66 19.9

11 Sabzazar SBZ-1 Taste less Colorless 7.47 33.6 1.85 19.8

12 Johr town JT-1 Taste less Colorless 6.71 66.0 4.81 19.0

13 Valencia

town

VT-1 Taste less Colorless 7.17 46.7 0.43 19.4

14 Model town MT-1 Taste less Colorless 7.26 42.7 1.67 21.1

3.8.1. SAMPLES ARE COLLETED FROM:

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3.9. PH CHART:

Figure 3.5 PH Chart

3.10. TURBIDTY CHART:

Figure 3.6 Turbidity Chart

PH

Location

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3.11. COMMENTS:

The PH of the samples lies in the drinkable range, but the turbidity may vary. Although the

turbidity becomes vary but still it is drinkable and may need filters to make it safer for the health.

3.12. REFRENCES:

Class lectures

www.google.com/physical parameters of the drinking water

http://www.lovibond.com/en/pool/turbidity-meters

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Figure 4.1 TDS Apparatus

LAB#04

4.1. STATEMENT:

DETERMINE THE TDS AND CONDUCTIVITY OF WATER SAMPLES BY USING

TDS METER AND CONDUCTIVITY METER

4.2. SCOPE:

- To determine the physical contamination (TDS and turbidity) of a given water sample

4.3. THEORY:

4.3.1. TOTAL DISSOLVED SOLIDS (TDS):

Total Dissolved Solids (TDS) are solids in water that can pass through a filter. TDS is a measure

of the amount of material dissolved in water. This material can include carbonate, bicarbonate,

chloride, sulfate, phosphate, nitrate, calcium, magnesium, sodium, organic ions, and other ions.

A certain level of these ions in water is necessary for aquatic life. Changes in TDS

concentrations can be harmful because the density of the water determines the flow of water into

and out of an organism's cells. However, if TDS concentrations are too high or too low, the

growth of much aquatic life can be limited, and death may occur. High concentrations of TDS

may also reduce water clarity, contribute to a decrease in photosynthesis, combine with toxic

compounds and heavy metals, and lead to an increase in water temperature.

TDS is used to estimate the quality of drinking water, because it represents the amount of ions in

the water. Water with high TDS often has a bad taste and/or high water hardness, and could

result in a laxative effect.

4.3.2. MEASUREMENT OF TOTAL DISSOLVED SOLIDS:

To measure TDS, the water sample is filtered, and then the filtrate (the water that passes through

the filter) is evaporated in a pre-weighed dish and dried in an oven at 180oC, until the weight of

the dish no longer changes. The increase in weight of the dish represents the total dissolved

solids, and is reported in milligrams per liter (mg/l).

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4.4. FACTORS AFFECTING TOTAL DISSOLVED SOLIDS

4.4.1. GEOLOGY AND SOIL IN THE WATERSHED:

Some rock and soil release ions very easily when water flows over them; for example, if acidic

water flows over rocks of calcareous shales, calcium (TDS will increase. However, some rocks,

such as quartz-rich granite, are very resistant to dissolution, and don’t dissolve easily when water

flows over them.

4.4.2. URBAN RUNOFF:

During storm events, pollutants such as salts from streets, fertilizers from lawns, and other

material can be washed into streams and rivers and dissolved solids are carried through storm

drains to creeks and rivers.

4.4.3. SOIL EROSION:

Soil erosion is caused by disturbance of a land surface. Soil erosion can be caused by Building

and Road Construction, Forest Fires, Logging, and Mining. The eroded soil particles may

contain soluble components that can dissolve and be carried by storm water to surface water.

This will increase the TDS of the water body.

4.4.4. DECAYING PLANTS AND ANIMALS:

As plants and animals decay, dissolved organic particles are released and can contribute to the

TDS concentration. High TDS concentrations in water are also unsuitable for many industrial

applications.

4.5. PROCEDURE:

Take beakers in which samples have to collect and clean them.

Note the weight the empty beakers.

Take different samples of water of 60 ml from different places in these beakers.

Again note the weight the beakers containing the water samples.

Now heat the beakers until all the water in the beakers evaporates.

After complete evaporation of water, note the weight of empty beakers again. Beakers before

and after the heating gives the value of TDS (total dissolved solids).

Now difference in the weights of empty

TDS = Wt. after heating – Wt. before heating

4.5.CONDUCTIVITY STANDARDS:

- Ultra-pure water = 5.5×10-6

s/m

- Drinking water = 0.005 – 0.05 s/m

- Sea water = 5 s/m

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4.6. TDS STANDARDS:

- Fresh water ˂1000 mg/l

- Breakish water = 1000 – 3000 mg/l

- Sea water = 73500 mg/l

4.7. OBSERVATION AND CALCULATIONS:

Sample

#

Sample

location

Sample

ID

Intial

weight

of

beaker

Final

weight

of

beaker

TDS

mg/l

(ppm)

Conductivity

µs/cm

TDS

Ppm

µs/cm 0.67

TDS

Meter

(ppm)

1 Cant 1 C-1 30.59 30.599 475 619 455 518

2 Cant 2 C-2 42.47 42.489 480 709 475 506

3 Cant 3 C-3 32.08 32.094 350 474 318 396

4 F.F.UET Uet-3 51.74 51.759 410 492 330 455

5 UET

Q hall

Uet-4 37.93 37.958 710 1044 699 769

6 Cant C-4 32.09 32.105 395 535 358 435

7 UET

L hall

Uet-1 30.147 30.1775 763 1063 712 807

8 UET SMG

hall

Uet-2 50.78 50.803 595 800 536 623

9 Shalimar

link road

SL-1 30.59 30.611 545 735 492 621

10 Shadara SHD-1 37.95 37.963 365 372 250 468

11 Sabzazar SBZ-1 37.8 37.967 235 262 176 213

12 Johr town JT-1 51.74 51.756 290 396 265 326

13 Valencia

town

VT-1 30.147 30151 816 1085 726 905

14 Model

town

MT-1 42.47 42.475 1017 1400 938 1169

4.8. W.H.O STANDARDS:

An aesthetic objective of ≤500 mg/L has been established for total dissolved solids (TDS) in

drinking water by WHO. At higher levels, excessive hardness, unpalatability, mineral deposition

and corrosion may occur. At low levels, however, TDS contributes to the palatability of

water. Most people think of TDS as being an aesthetic factor. In a study by the World Health

Organization, a panel of tasters came to the following conclusions about the preferable level

of TDS in water:

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4.9. TDS CHART:

Figure 4.2 TDS Chart

Taste of Water with Different TDS Concentrations

Level of TDS (milligrams per litre) Rating

Less than 300 Excellent

300 - 600 Good

600 - 900 Fair

900 - 1,200 Poor

Above 1,200 Unacceptable

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4.10. REFRENCES:

- www.En.wikipedia.org/wiki

- www.water-research.net

- Lab briefing

- https://water.tallyfox.com/answer/permissible-range-total-dissolve-solid-tds-drinking-water

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Figure 5.2 Paqualab Photometer

LAB#05

5.1. STATEMENT:

DETERMINE QUANTITY OF POTASIUM (K) AND MAGNESIUM (Mg)

IN GIVEN WATER SAMPLE

5.2. APPARATUS:

Paqualab Photometer

5.3. RELATED THEORY

5.3.1. PAQUALAB PHOTOMETER

A photometer is an instrument for measuring light intensity or optical properties of solutions or

surfaces. Depending on the purpose, a variety of filters can be used to measure light of specific

wavelengths. A photometer can also be used to measure ambient light, or only a narrow beam

that comes directly from a source such as the Sun.

5.3.2. IMPORTANCE OF MAGNESIUM:

Magnesium is one of the most important minerals found in human’s body, which is involved in

cellular energy production, enzyme activity; it also regulates the spread of nerve impulses.

Magnesium is important for muscular (especially when one’s got crumps) and nervous system

activity, also for the bone structure. Magnesium regulates the metabolism of other minerals; it

also controls the uptake of such substances as: calcium, potassium, phosphorus, copper, vitamin

C and zinc.

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5.3.3. IMPORTANCE OF POTASSIUM:

Potassium is the most important cation in human’s body; it is also called mineral of the heart. It

regulates blood pressure, maintains normal fluid balance, it is important for nervous system and

heart muscle work, potassium acts as an electrolyte. Potassium directly affects the heart muscle

cells and nerve impulse function, therefore cardiac contractions change when potassium level

changes. It was estimated that consuming more potassium decreases level of risk hypertension

related diseases- stroke, heart attack and kidney failure.

5.3.4. DRINKING WATER:

Drinking water (or potable water) is water safe enough to be consumed by humans or used with

low risk of immediate or long term harm. Over large parts of the world, humans have inadequate

access to potable water and use sources contaminated with disease vectors, pathogens or

unacceptable levels of toxins or suspended solids. Drinking or using such water in food

preparation leads to widespread acute and chronic illnesses and is a major cause of death and

suffering worldwide in many different countries. Reduction of waterborne diseases and

development of safe water resources is a major public health goal in developing countries.

5.3.5. SOURCES OF POTASIUM:

Seawater contains about 400 ppm potassium. It tends to settle, and consequently ends up in

sediment mostly. Rivers generally contains about 2-3 ppm potassium. This difference is mainly

caused by a large potassium concentration in oceanic basalts

Potassium occurs in various minerals, from which it may be dissolved through weathering

processes. Examples are feldspars (orthoclase and microcline) and chlorine minerals which are

most favorable for production purposes. Some clay minerals contain potassium.

5.3.6. SOURCES OF MAGNESIUM:

Magnesium is present in seawater in amounts of about 1300 ppm. After sodium, it is the most

commonly found cation in oceans. Rivers contains approximately 4 ppm of magnesium. Dutch

drinking water contains between 1 and 5 mg of magnesium per liter.

A large number of minerals contains magnesium, for example dolomite and magnesite.

Magnesium is washed from rocks and subsequently ends up in water. Magnesium has many

different purposes and consequently may end up in water in many different ways.

5.3.6. W.H.O STANDARDS:

Magnesium Mg 50.0 mg/L

Potassium K no limit listed

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5.4. PROCEDURE

Take 10 ml of sample and put that into the test tube.

Take one potassium tablet, crushed and dissolved it in the sample.

A cloudy solution will form that indicates the presence of potassium.

Keep solution for 5 minutes without agitation.

Take another test tube and add some distilled water in it.

Now turn on the Paqualab and put the distilled water test tube in it while it will show the

reading 100.

Then immediately take out the distilled water and put the sample water test tube in the

Paqualab.

Note down the reading when it becomes constant.

By using the table find out the value of potassium in milligram.

Figure 5.2 Water Samples

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5.5. D ATA:

Sr.

No.

Sample

Location

Sample

ID

“K” Concentration “Mg” Concentration

Paqualab

reading

(%age)

Table

reading

(mg/L)

Remarks Paqualab

reading

(%age)

Table

reading

(mg/L)

Remarks

1 Shahdra SH-1 32 5.6 Slightly

hard

64 9.0 Moderately

hard

2 Johar

town

JT-1 92 1.8 Moderately

hard

60 10.0 Moderately

hard

3 Shalimar SH-2 36 5.0 Slightly

hard

56 11.5 Slightly hard

4 Cant-III C-III 39 4.7 Slightly

hard

62 9.5 Moderately

hard

5 Q-hall UET-5 39 4.7 Slightly

hard

61 10.0 Moderately

hard

6 Sabzazar SB-1 57 3.5 Slightly

hard

60 10.0 Moderately

hard

7 Model

town

MT-1 25 6.8 Slightly

hard

69 7.0 Moderately

hard

8 SMG UET-2 36 5.0 Slightly

hard

62 9.5 Moderately

hard

9 L-hall UET-1 35 5.2 Slightly

hard

63 9.5 Moderately

hard

10 Valencia V-1 44 4.3 Slightly

hard

64 9.0 Moderately

hard

11 FF-UET UET-4 69 2.9 Moderately

hard

60 10.0 Moderately

hard

12 Cant-IV C-IV 37 4.9 Slightly

hard

64 9.0 Moderately

hard

13 Cant-I C-I 41 4.5 Slightly

hard

59 10.5 Slightly hard

14 Cant-II C-II 36 5.0 Slightly

hard

61 10.0 Moderately

hard

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GRAPH FOR K CONCENTRATION:

Figure 5.3 K Concentration Graph

GRAPH FOR Mg CONCENTRATION:

Figure 5.4 Mg Concentration Graph

0

1

2

3

4

5

6

7

Co

nce

ntr

atio

n o

f K

Location

K Concentration

0

2

4

6

8

10

12

Mg

Co

nce

ntr

atio

n

Location

Mg Concentration

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5.6. PRECAUTIONS:

Sample bottles were either rinsed three times with source water before collecting the sample

or rinsed with 70% methanol to minimize the risk of external contamination.

Prior to sample collection, the well was pumped for approximately 10 minutes. This exercise

ensured that the sample was representative of the aquifer and not the standing water in the

well.

5.7. REFRENCES:

- Literature: Joseph E. Zerwekh, a, , Clarita V. Odvinaa, Lisa-Ann Wuermser, a and Charles

Y.C. Paka Center for Mineral Metabolism and Clinical Research, and the Department of

Physical Medicine and Rehabilitation, University of Texas Southwestern Medical Center at

Dallas, Texas

- http://en.wikipedia.org/wiki/Drinking_water

- http://www.livestrong.com/article/86292-foods-rich-potassium-magnesium/

- http://en.wikipedia.org/wiki/Drinking_water_quality_standards

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LAB#06

6.1. STATEMENT:

TO FIND THE DIRECTION OF GROUND WATER MOVEMENT AND HYDRAULIC

GRADIENT OF WELLS BY USING THREE POINTS METHOD

6.2. PROCEDURE:

1. Choose an intermediate well

2. Project well 1 on line between well 2 and well 3.

3. Flow line is perpendicular to equipotential line, so draw a perpendicular from well 2 to the

equipotential line.

4. Now find out the hydraulic gradient by using the following formula:

i =

i =

It shows that the direction of the ground water is towards Well 2.

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Figure 7.5 Multi gas detector

LAB#07

TO FIND OUT THE ENVIRONMENTAL CONDITION BY USING MULTIGAS

DTECTOR

7.1. APPARATUS:

Multi gas detector

7.2. RELATED THEORY:

7.2.1. MULTI GAS DETECTOR:

A gas detector is a device which detects the presence of various gases with in an area usually as

part of the system to warn about the gases which might be harmful to humans and animals. Gas

detectors can be used to detect combustable toxic oxygen and carbon dioxide gases.

7.2.2. PRINCIPLE:

Gas detectors measure and indicate the concentration of certain gases in an air via different

technologies. Typically employed to prevent toxic exposure and fire, gas detectors are often

battery operated devices used for safety purposes. They are manufactured as portable or

stationary (fixed) units and work by signifying high levels of gases through a series of audible or

visible indicators, such as alarms, lights or a combination of signals. While many of the older,

standard gas detector units were originally fabricated to detect one gas, modern multifunctional

or multi-gas devices are capable of detecting several gases at once. Some detectors may be

utilized as individual units to monitor small workspace areas, or units can be combined or linked

together to create a protection system.

As detectors measure a specified gas concentration, the sensor response serves as the reference

point or scale. When the sensors response surpasses a certain pre-set level, an alarm will activate

to warn the user. There are various types of detectors available and the majority serves the same

function: to monitor and warn of a dangerous gas level. However, when considering what type of

detector to install, it is helpful to consider the different sensor technologies.

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7.2.3. TYPES OF RISKS FROM GASES:

Basically there are three categories of risk

EX:

Risk of explosion by flammable gases

OX:

Oxygen Risk of increase of flammability by oxygen enrichment

TOX:

Risk of poisoning by toxic gases.

7.4. PROCEDURE:

The procedure for the multi gas detector is very simple.

- Before taking the reading one should calibrate the apparatus to get the exact readings.

- After calibrating the apparatus is kept in air and the measure button is pressed.

- The apparatus shows the readings of different gases with a small time of interval.

- There is an alarm which starts sounding when there is efficiency in the level of gases.

7.5. OBSERVATIONS & CALCULATIONS:

S. No Gases to be Measured Normal Values Observed Values

1 O2 20.95% 20.60%

2 CO Trace (0.05% is fatal) -

3 CO2 0.038% 0.04%

4 NO Trace -

5 NO2 0.02 ppm -

7.6. APPLICATIONS:

The followings are the applications of Multi Gas Detector

- Pulp and paper industry

- Refineries and petrochemical plants including offshore drilling and plant shutdowns

- Sewers and manholes

- Coalmines

- Landfills operations

- Waste water treatment plant

- Marine and offshore oil wells

- Trenches and railcars

- Tunnels

- Power plants

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7.8. COMMENTS:

The multi gas monitor instrument was out of order so we can’t perform the experiment by

ourselves. We only took the readings which were provided by our teacher.

7.9. REFRENCES:

- Notes of University of Saskatchewan lab manual

- http://www.jjstech.com/gasdepr.html

- Class notes

- http://www.thomasnet.com/articles/instruments-controls/How-Gas-Detectors-Work

- http://en.wikipedia.org/wiki/Gas_detector

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LAB#08

8.1. STATEMENT:

ESTIMATION OF MICROBIOLOGICAL CONTAMINATION IN WATER SAMPLES

BY USING PAQUALAB

8.2. SCOPE:

Checking of the suitability of the water on the basis of the biological parameters testing of the

drinking water collected from the different localities of Lahore city.

APPARATUS:

- Paqualab

- Filter papers

- Suction pump beaker

- Steel paltes

- Lauryl sulphate

- Hot plate

- Water samples

Figure 8.2 Total Apparatus Figure 8.6 Paqualab

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8.3. RELATED THEORY:

8.4. COLIFORM BACTERIA:

Coliform bacteria are commonly found in soil, on vegetation, and in surface water. They also

live in the intestines of warm-blooded animals and humans. Some coliform bacteria strains can

survive in soil and water for long periods of time. Coliform bacteria will not likely cause illness.

However, because coliform bacteria are most commonly associated with sewage or surface

waters, the presence of coliform bacteria in drinking water indicates that other disease-causing

organisms (pathogens) may be present in the water system. There are main two different groups

of coliform bacteria, each has a different level of risk.

Total Coliform

Fecal Coliform

8.4.1. TOTAL COLIFORM:

These types of bacteria are commonly found in the environment (e.g. soil or vegetation) and are

generally harmless. If only total coliform bacteria are detected in drinking water, the source is

probably environmental, and fecal contamination is not likely. However, if environmental

contamination can enter the system, there may be a way for other pathogens to enter the system.

Therefore, it is important to determine the source and resolve the problem.

8.4.2. FECAL COLIFORM:

These types of bacteria are a sub-group of the total coliform group. They appear in great

quantities in the intestines and feces of people and animals. The presence of fecal coliform in a

drinking water sample often indicates recent fecal contamination, meaning that there is a greater

risk that pathogens are present than if only total coliform bacteria are detected.

8.4.3. DETECTION OF COLIFORM IN THE DRINKING WATER:

Since bacterial contamination cannot be detected by taste, smell, or sight, all drinking water

wells should be tested at least annually for coliform bacteria. A coliform bacteria test is also

recommended immediately if:

• A sudden change occurs in your water’s taste, appearance or odor.

• The water turns cloudy after rainfall or the top of the well was flooded.

• Family members are experiencing unexplained flu-like symptoms.

Figure 8.3 Water Samples

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8.5. PROCEDURE:

1. Take lauryl tryptose sulphate broth of 1.05g for 30 ml of water

2. Boil it for 15 minutes at 121 C

3. Add a few drops of lauryl sulphate to each filter paper

4. Pour 100 ml of water through that paper

5. Using suction pump pull the water towards the beaker

6. The filter paper is collected and kept on the Paqualab for 24 hours

7. The bacteria can be seen the paper

8. The blue colour shows foli coliform and the pink shows coliform

TABLE#8.1:

Sample No. Sample

Location

Sample

ID

Container

No.

No. of

Bacteria

Colony

WHO/

Remarks

1 Shahdra SH-1 B-2 0 Drinkable

2 Johertwon JT-1 B-6 0 Drinkable

3 Shalimar SH-2 S-4 0 Drinkable

4 Cantt-III C-III B-4 2 Not Drinkable

5 Q-hall UET-5 B-3 0 Drinkable

6 Sabzazar SB-1 B’-3 0 Drinkable

7 Model town MT-1 B-11 0 Drinkable

8 SMG UET-2 S’-1 0 Drinkable

9 L-hall UET-1 S-6 0 Drinkable

10 Vlencia V-1 S-11 0 Drinkable

11 FF-uet UET-4 S-3 0 Drinkable

12 Cant-IV C-IV S-10 0 Drinkable

13 Cant-I C-I B-9 0 Drinkable

14 Cant-II C-II S-1 0 Drinkable

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BACTERIA COLONY IN CANTT-ΙΙΙ:

8.6. PRECAUTIONS:

Only blue and pink colour should be noted only

The expiry date should be checked on the sulphate before using

The sample should not be kept for than 24 hours

8.7. COMMENTS:

The lauryl sulphate used in this lab was expired and hence the results were not accurate. Also the

cantt-ΙΙΙ were noted to have bacteria which shows that it is considered to be not drinkable.

8.8. REFRENCES:

- https://www.health.ny.gov/environmental/water/drinking/coliform_bacteria.htm

- http://en.wikipedia.org/wiki/Coliform_bacteria

- http://en.wikipedia.org/wiki/Fecal_coliform

- http://www.ncbi.nlm.nih.gov/pubmed/11777581

Figure 8.7 Cantt 3 Bacteria

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LAB#09

9.1. STATEMENT:

DIRECTIONS OF GROUND WATER FLOW WITHIN MAPPED AREA

9.2. DATA:

Well No. Surface Elevation

(ft.)

Water Table (ft.) Elevation of water

table (ft.)

1 755 55 700

2 740 60 680

3 710 20 690

4 690 10 680

5 690 30 660

6 750 50 700

7 730 70 640

8 780 72 708

9 730 30 700

10 760 60 700

11 780 60 720

12 750 70 680

13 760 35 725

14 750 30 720

15 750 50 700

16 740 25 715

17 725 25 700

18 750 50 700

19 760 65 695

20 680 50 630

21 660 50 610

22 740 60 680

23 700 40 660

24 700 25 675

25 710 30 680

26 720 55 665

A 750 60 690

B 750 100 650

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9.3. ANSWERS:

Ans 10-9:

From Well A to Well B

Ans 10-10:

A) Well B (100 ft.)

B) Spring occours when peizometric surface is above or near the surface elevation

Ans 10-11:

The waste finds its way towards Jones Farm house

Ans 10-12:

40 ft.

Ans 10-13:

1000 ft.

Ans 10-14:

i =

i= 0.04

Ans 10-15:

Q= KiA

= 10×0.04×(200×5000)

= 400,000 ft3/day

9.4. COMMENTS:

For the complete questions see the page attached with this lab. Also a contour map is being

drawn which shows elevation of the same height or depth.