endomembranes & protein trafficking chapter 8 part 1
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TRANSCRIPT
Endomembranes &
Protein Trafficking
Chapter 8
Part 1
Major Intracellular Compartments of the Cell
Eucaryotic cell Compartments
Simplified Road Map of Protein Traffick
Protein Trafficking
Approaches to the Study of Endomembranes
Autoradiography: Site & Synthesis of Secretory Proteins
The Secretory Pathway:Pancreatic Acinar Cell: Autoradiography showing
movement of labeled secretory proteins (red color = black silver grains)
Use of GFP reveals movement of proteins within a living cell
Use of GFP reveals movement of proteins within a living cell
Biochemical Analysis of Subcellular Fractions
Cell-Free SystemsFormation of Coated Vesicles using liposomes incubated with
protein components identified to be required for vesicle budding in a cell
Use of Genetic Mutants in the Study of Secretion: The Yeast Model
The Endoplasmic Reticulum (ER)
Polarized Structure of a Secretory CellMucus-secreting cells from Rat Colon
Overview of Protein Sorting
Isolation of Rough ER (Microsomes)
The Signal Hypothesis ExperimentIncorporation of Secretory Proteins into Microsomes
The Signal Hypothesis ExperimentIncorporation of Secretory Proteins into Microsomes
The Signal Hypothesis Experiment Incorporation of Secretory Proteins into Microsomes SDS-Gel Electrophoresis Analysis
Translation on free ribosomes Translation on Microsomes
Secretory ProteinSecretory Protein
The Signal Hypothesis Continued
The Signal Hypothesis Continued
The Signal Sequence of Growth Hormone
Signal Peptidase Processing Enzyme located in ER
Schematic Model of the synthesis of a Secretory Protein (or a lysosomal enzyme) on an Membrane-Bound ribosome of the RER.
Role of GTP Hydrolysis in SRP and SRP Receptor Function
Only for General Knowledge
Only for General KnowledgeSchematic Model for the Synthesis of an Integral Membrane Protein
Only for General Knowledge Maintenance of Membrane Asymmetry
Only for General Knowledge Modifying the Lipid Composition of Membranes
Only for General KnowledgeSynthesis of the core portion of an N-linked oligosaccharide in the rough ER
Quality Control: Ensuring that Misfolded proteins do not proceed forward
Model of the Mammalian Unfolded Response UPR