enantiomers of lipoic acid differentially affect nrf2 activation
DESCRIPTION
Enantiomers of Lipoic Acid Differentially Affect Nrf2 Activation. Kelly Crotty Dr. Tory Hagen. Our bodies are under attack from both external and internal toxins. Nrf2: A Transcription Factor. B zip. Transcription factor Binds to the A ntioxidant R esponse E lement (ARE). - PowerPoint PPT PresentationTRANSCRIPT
•Transcription factor
• Binds to the Antioxidant Response Element (ARE)
•Influences over 200 genes with the ARE
•These genes produce detoxification enzymes and antioxidant proteins
Nrf2: A Transcription Factor
B zip
• Always being produced
• Almost immediately bound to the protein Keap1 and degraded
Nucleus
Cell
Nrf2 Production and Degradation
Nucleus
Cell
Nrf2 Activation
• Under stress, Nrf2 returns to the nucleus in greater
amounts to stimulate gene expression.
How potent is S-LA compared to the commercially available racemic mixture or natural R-LA?
Question:
Methods:
Measure relative Nrf2 protein levels in the nucleus through western blot analysis
Isolate the nuclei of cells that have been treated with lipoic acid
R-LA > R,S-LA > S-LA
in activating Nrf2
Implications:
Lipoic
Acid •Though R-LA and S-LA have similar
structure, they activate Nrf2 at
completely different concentrations
Future Projects:
• Continue the concentration curve until R,S-LA and S-LA
have also reached saturation
• Add more time points for the time course
• Repeat the time course with R-LA