emmonya biotech catalog

Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©

2

INDEX

1. Hematoxylin stains ................................... 4

2. Eosin ................................... 6

3. Staining solutions for histology and cytology ................................... 8

4. Reagents I ................................... 48

5. Reagents II ................................... 55

6. Decalcifying solutions ................................... 58

7. Fixatives ................................... 59

8. Formalins ................................... 59

9. Solvents ................................... 60

10. Mounting and embedding media ................................... 60

11. Legend ................................... 61


3

Dear distributors, suppliers and customers,

We are pleased to present our new catalog “Microscopic stains and reagents for cytology, histology and microbiology”

Emmonya Biotech


4

1. Hematoxylin stains

Products Code Size DescriptionGill’s hematoxylin I 111601350 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei.111601310 1lGill’s hematoxylin II 111601450 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei.111601410 1lGill’s hematoxylin III 111601550 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei111601510 1l

Gill I Gill II Gill III


5

Mayer’s hematoxylin 111600250 500ml For standard haematoxylin-eosin staining of tissue sections. For nuclei111600210 1l

Harris’ hematoxylin 111600450 500ml For standard haematoxylin-eosin staining of tissue sections. For nuclei111600410 1l

Weigert’s Hematoxylin 130880100 100 test For trichrome stains130880250 250ml130880500 500ml130880010 1l

Harris Mayer Weigert


6

2. Eosin

Products Code Size DescriptionEosin Y 1% aqueous sol. 111000050 50ml Standard haematoxylin-eosin staining

for cytoplasm111000300 300ml111000250 500ml111000210 1l

Eosin Y 5% aqueous sol. 111000050.5 50ml Standard haematoxylin-eosin staining for cytoplasm111000250.5 500ml

111000210.5 1l

Eosin Y 1% aqueous sol. Eosin Y 5% aqueous sol.


7

Eosin Y 0.5% alcoholic sol. 111000305 50ml Standard haematoxylin-eosin staining for cytoplasm111000325 250ml

111000350 500ml111000310 1l

Eosin-Phloxin B alcoholic sol. 111002005 50ml Standard haematoxylin-eosin staining for cytoplasm111002025 250ml

111002050 500ml111002010 1l

Eosin Y 0,5% alcoholic sol. Eosin-Phloxin B alcoholic sol.


8

3. Staining solutions for histology and cytology

Products Code Size DescriptionAlcian blue pH1 162802050 100test For demonstration of acid mucins in

tissue sections162802250 250ml162802500 500ml162802100 1l

Alcian blue pH2.5 161802050 100test For demonstration of carboxylated and some weakly sulfated mucins in tissue sections

161802250 250ml161802500 500ml161802100 1l

Alcian blue pH1 - P.A.S. 111200100.1 100test For neutral and acid mucins in tissue sections111200101.1 100ml

111200250.1 250ml111200500.1 500ml111200010.1 1l

Alcian blue pH2.5 - P.A.S. 111200100 100test For neutral and acid mucins in tissue sections111200101 100ml

111200250 250ml111200500 500ml111200010 1l


9

Alcian Yellow - Toluidine Blue 111300100 100test For screening patient specimens for Helicobacter pilory111300250 250ml

Azan Trichrome 121111100 100test Method for connective tissue, especially to show muscle fibers, collagen, reticulum, kidney glomerular stroma in tissue sections

121111250 250 ml121111010 1l

Brilliant Cresyl Blue for reticulocytes

111400050 100test Blood smears examination for reticulocytes111400125 125ml

111400250 250ml111400500 500ml111401000 1l

Bennhold’s Congo red for amyloid 131003050 100test Stain for amyloid deposits in tissues131003250 250ml131003500 500ml131003100 1l

Highman’s Congo red for amyloid 131103205 100test Stain for amyloid deposits in tissues131103250 250ml131103500 500ml131103100 1l

CytoMax - fast Papanicolau for cyto PAP smear

221222050 100test For cyto PAP and other smears (cytological specimens)221222100 100ml

221222250 250ml221222500 500ml


10

Giemsa stain 111120100 100test Giemsa concentrate solution111120250 250ml111120500 500ml111120010 1l

Gomori silver impregnation 114080100 100test Method to show argyrophilic reticular fibers in connective tissue

Gram for microbiology 100802100 100test Differentiation between Gram positive and Gram negative bacteria in different specimens

100802125 100ml100802250 250ml100802500 500ml100802010 1l

Grocott’s methenamine silver nitrate method for fungi

143823100 100test To identify fungi in tissue section143823010 100ml

Haematocolor (hematocolor) 111316050 100test Blood smear examinations111316100 100ml111316250 250ml111316500 500ml

Leishman stain 113273100 100test Blood smear examinations113273125 125ml113273250 250ml113273500 500ml


11

Leishman-Wright stain 113273100W 100test Blood smear examinations113273125W 125ml113273250W 250ml113273500W 500ml

Mallory trichrome 120802100 100test Staining solution for connective tissueMasson trichrome Goldner 110802100.1 100test Staining solution for connective

tissue - collagen and muscle fiberMasson trichrome Standard 110802100 100test Staining solution for connective

tissue - collagen and muscle fiberMay Grunwald 111200100 100test Fixative for MGG blood smear

examinations111200250 250ml111200500 500ml111200010 1l

May Grunwald - Giemsa 118080100 100test Blood smear examination118080010 1l

Methenamine Silver Impregnation (P.A.S.M.)

114382100 100test Kit for histology (lamina basalae, argyrophil cells etc.).

Methylene Blue Loffler 112000100 100test Alkaline stain for cell nuclei in smears from different origin and pathogens112000250 250ml

112000500 500ml112000010 1l


12

New Methylene Blue for reticulocytes

1114003050 100test Blood smear examination for reticulocytes1114003125 125ml

1114003250 250mlOil Red O for lipids in frozen sections

122092100 100test Lipid staining in frozen sections 122092125 125ml122092250 250ml

Orcein 155802100 100test To demonstrate elastic fibers in tissue sections, recomended for vascular pathology

155802250 250ml

Papanicolau - OG6 112220250 250ml Stain solution I for cyto PAP smears112220500 500ml112220010 1l

Papanicolau - EA65 112230250 250ml Stain solution II for cyto PAP smears112230500 500ml112230010 1l

Papanicolau - EA50 112230250.1 250ml Stain solution II for cyto PAP smears112230500.1 500ml11223010.1 1l

Periodic Acid Schiff (P.A.S.) 113080100 100test Examination of mucosubstances (mucopolysaccharides), lamina basalae, glycogen deposits in liver etc. in tissue sections

113080101 100ml113080250 250ml113080500 500ml


13

Rapihem 111317050 100test Blood smears, other cytological specimens and fine needle aspirations examination

111317250 250ml111317500 500ml111317100 1l

Sudan III Propylene Glycol 112700050 100test Triglyceride staining in frozen sections112700250 250ml112700500 500ml112700100 1l

Sudan III alcohol solution 112700050A 100test Staining of triglycerides in frozen tissue sections.112700250A 250ml

112700500A 500ml112700100A 1l

Toluidine Blue stain for hystology 123001050 100test To demonstrate metachromatic substances and mast cells in tissue sections

123001250 250ml123001500 500ml123001100 1l

Van Gieson Trichrome stain 130802100 100test Staining for connective tissue130802125 125ml130802250 250ml130802500 500ml

Weigert for elastic fibers 124001100 100test To demonstrate elastic fibers in tissue sections124001125 125ml


14

Wright’s Stain for cytology 113273100WP 100test Fine needle aspirations and other cytological specimens examination113273125WP 125ml

113273250WP 250ml113273500WP 500ml

Ziehl Neelsen cold 116450050 100test Microscopic examination of Mycobacteria116450250 250ml

116450500 500ml

15

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21

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NH

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23

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e if

cont

ra-s

tain

ed

S

tain

for a

myl

oid

depo

sits

in ti

ssue

s. S

uita

ble

for p

araf

fin s

ectio

ns fi

xed

in

10%

form

alin

. Am

yloi

d de

posi

ts a

re fo

rmed

in ti

ssue

s sp

onta

neou

sly

or in

ass

ocia

tion

with

a w

ide

rang

e of

dis

ease

s. In

cas

es o

f sus

pect

ed a

myl

oido

sis,

thic

k se

ctio

ns a

t 12

μm m

ay b

e be

nefic

ial i

n de

mon

stra

ting

min

ute

depo

sits

of a

myl

oid.

Rea

gent

s:

A- C

ongo

red

stai

ning

sol

utio

n ac

cord

ing

Hig

hman

B- P

otas

sium

hyd

roxi

de s

olut

ion

Hig

hman

’s c

ongo

red

for a

myl

oid

- kin

dey

24

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

CYT

OM

AX

num

ber o

f tes

ts10

0pr

oced

ure

time

3 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

Nuc

lei

Viol

et-b

lue

to b

lack

Cyt

opla

sm (e

osin

ophi

lic)

Pin

kC

ytop

lasm

(bas

ophi

lic)

Turq

uois

e to

blu

eC

ytop

lasm

(met

apla

sia

cells

)W

ith b

oth

blue

and

pin

kE

ritro

cyte

sR

edM

icro

orga

nism

sB

lue-

viol

et

S

tain

sol

utio

n fo

r cyt

o PA

P an

d ot

her s

mea

rs (c

ytol

ogic

al s

peci

men

s).

Rea

gent

s:

А –

Har

ris`s

hae

mat

oxyl

in s

olut

ion

C –

Mod

ified

pol

ychr

omic

EA

65 s

olut

ion

B –

2-P

ropa

nol A

bsol

ute

D –

80%

2-P

ropa

nol

Cyt

omax

- cy

to P

AP

smea

r

25

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

GIE

MSA

STA

IN

num

ber o

f tes

ts10

0pr

oced

ure

time

~40

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

- 25

°C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

Lym

phoc

ytes

Ligh

t blu

e cy

topl

asm

Mon

ocyt

esU

sha

ped

nucl

eus

Gra

nulo

cyte

sN

eutro

phils

(3-6

lobe

d nu

cleu

s), e

osin

ophi

ls (2

lobe

d nu

-cl

eus)

, bas

ophi

ls (w

ith d

ark

viol

et to

bla

ck g

ranu

les)

Pla

tele

tsVi

olet

Erit

rocy

tes

Ligh

t red

Blo

od p

aras

ites

Red

B

lood

sm

ears

exa

min

atio

n.

Rea

gent

s:

Gie

msa

con

cent

rate

sol

utio

n

Gie

msa

sta

in -

bloo

d sm

ear

26

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

GO

MO

RI S

ILVE

R IM

PREG

NAT

ION

Gom

ori s

ilver

impr

egna

tion

- kid

ney

num

ber o

f tes

ts10

0pr

oced

ure

time

~30

min

.pr

oduc

t val

idity

1 ye

ar (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15-2

5°C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, i

n gl

ass

dark

bot

tle,

tight

ly c

lose

d

Ret

icul

ar fi

bers

and

ner

vous

cel

ls

Bla

ckC

onne

ctiv

e tis

sue

Toba

cco

brow

nC

olla

gen

Gol

d ye

llow

R

ecom

men

ded

met

hod

to s

how

arg

yrop

hilic

retic

ular

fibe

rs in

con

nect

ive

tis-

sue,

esp

ecia

lly to

diff

eren

tiate

col

lage

n fib

ers

from

con

nect

ivum

.

Rea

gent

s:

А –

Pot

assi

um p

erm

anga

nate

sol

utio

nС

– O

xalic

aci

d so

lutio

nE

– A

mm

onia

cal s

ilver

sol

utio

nG

– S

odiu

m h

ypos

ulph

ite s

olut

ion

В –

Aci

d ac

tivat

ing

buffe

rD

– F

erric

am

mon

ium

sul

fate

sol

utio

n F

– N

eutra

l for

mal

in s

olut

ion

27

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

GR

AM

num

ber o

f tes

ts10

0pr

oced

ure

time

5 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

Gra

m p

ositi

ve m

icro

orga

nism

sB

lue

Gra

m n

egat

ive

mic

roor

gani

sms

Red

D

iffer

entia

tion

betw

een

Gra

m p

ositi

ve a

nd G

ram

neg

ativ

e ba

cter

ia in

diff

eren

t sp

ecim

ens.

Rea

gent

s:

A –

Cry

stal

Vio

let s

olut

ion

C –

Dec

olor

isin

g so

lutio

nB

– L

ugol

’s s

olut

ion

for G

ram

D –

Saf

rani

n O

alc

ohol

ic s

olut

ion

Gra

m s

tain

ing

- bac

teria

l spe

cim

ents

28

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

GR

OC

OTT

for f

ungi

Gro

cott’

s m

etho

d fo

r fun

gi -

gast

ric ti

ssue

num

ber o

f tes

ts10

0pr

oced

ure

time

~100

min

. (~2

5 m

in. –

mic

row

ave

oven

mod

ifica

tion)

prod

uct v

alid

ity1,

5 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s4°

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

Fung

iB

lack

Muc

in

Gre

yB

ackg

roun

d G

reen

To

iden

tify

fung

i in

tissu

e se

ctio

ns. T

he m

ucop

olys

acch

arid

e co

mpo

nent

s of

th

e fu

ngal

cel

l wal

l are

oxi

dize

d to

rele

ase

alde

hyde

gro

ups.

The

ald

ehyd

e gr

oups

then

re

act w

ith th

e si

lver

nitr

ate,

redu

cing

it to

a m

etal

lic s

ilver

, ren

derin

g th

em v

isib

le.

Rea

gent

s:

А –

Chr

omic

aci

d so

lutio

nС

– M

ethe

nam

ine

solu

tion

E –

Bor

ax w

ater

sol

utio

nG

– S

odiu

m th

iosu

lfate

sol

utio

n

В –

Sod

ium

hyd

roge

nsul

fite

solu

tion

D –

Silv

er n

itrat

e so

lutio

nF

– G

old

chlo

ride

wat

er s

olut

ion

H –

Lig

ht G

reen

sol

utio

n

29

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

HA

EMAT

OC

OLO

R (H

EMAT

OC

OLO

R)

num

ber o

f tes

ts10

0pr

oced

ure

time

1 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

B

lood

sm

ears

exa

min

atio

n.

Rea

gent

s:

A –

Met

hano

l abs

olut

eC

– H

aem

atoc

olor

Sol

utio

n II

B –

Hae

mat

ocol

or S

olut

ion

ID

– H

aem

atoc

olor

Pho

spha

te b

uffe

r

Lym

phoc

ytes

Blu

ish

Mon

ocyt

esU

sha

ped

nucl

eus

Gra

nulo

cyte

sN

eutro

phils

(3-6

lobe

d nu

cleu

s), e

osin

ophi

ls (2

lobe

d nu

-cl

eus)

, bas

ophi

ls (d

ark

stai

ned

due

to th

e la

rge

hist

amin

e gr

anul

es)

Pla

tele

tsP

urpl

eE

ritro

cyte

sP

urpl

e-pi

nkis

h

Hae

mat

ocol

or -

bloo

d sm

ear

30

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

LEIS

HM

AN

STA

IN

num

ber o

f tes

ts10

0pr

oced

ure

time

7 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

B

lood

sm

ears

exa

min

atio

n.

B –

Buf

fere

d w

ater

Rea

gent

s:

A –

Leis

hman

sta

inin

g so

lutio

n

Leis

hman

sta

in -

bloo

d sm

ear

Lym

phoc

ytes

Blu

ish

Mon

ocyt

esU

sha

ped

nucl

eus

Gra

nulo

cyte

sN

eutro

phils

(3-6

lobe

d nu

cleu

s), e

osin

ophi

ls (2

lobe

d nu

-cl

eus)

, bas

ophi

ls (d

ark

stai

ned

due

to th

e la

rge

hist

amin

e gr

anul

es)

Pla

tele

tsP

ink-

viol

etE

ritro

cyte

sP

ink

31

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

LEIS

HM

AN

-WR

IGH

T ST

AIN

num

ber o

f tes

ts10

0pr

oced

ure

time

7 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

B

lood

sm

ears

exa

min

atio

n.

B –

Buf

fere

d w

ater

Rea

gent

s:

A –

Leis

hman

-Wrig

ht s

tain

ing

solu

tion

Leis

hman

-Wrig

ht s

tain

- bl

ood

smea

r

Lym

phoc

ytes

Blu

ish

Mon

ocyt

esU

sha

ped

nucl

eus

Gra

nulo

cyte

sN

eutro

phils

(3-6

lobe

d nu

cleu

s), e

osin

ophi

ls (2

lobe

d nu

-cl

eus)

, bas

ophi

ls (d

ark

stai

ned

due

to th

e la

rge

hist

amin

e gr

anul

es)

Pla

tele

tsP

ink-

viol

etE

ritro

cyte

sP

ink

32

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MA

LLO

RY T

RIC

HR

OM

E

num

ber o

f tes

ts10

0pr

oced

ure

time

~20

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

- 25

°C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

Nuc

lei

Red

Ery

thro

cyte

s O

rang

e re

dM

uscl

e R

edC

olla

gen

Blu

e

S

tain

ing

solu

tion

for c

onne

ctiv

e tis

sue.

Rea

gent

s:

А –

Aci

d fu

chsi

n st

aini

ng s

olut

ion

C –

Mal

lory

’s s

tain

ing

solu

tion

B –

Pho

spho

mol

ybdi

c ac

id s

olut

ion

Mal

lory

tric

hrom

e - p

lace

nta

33

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MA

SSO

N T

RIC

HR

OM

E ST

AN

DA

RD

num

ber o

f tes

ts10

0pr

oced

ure

time

~40

min

(+/-

pre-

fixat

ion

time)

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

- 25

°C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

Nuc

lei

Bla

ckM

uscl

e fib

ers,

cyt

opla

sm, e

ryth

rocy

tes

Red

Col

lage

n, m

ucus

Blu

e

S

tain

ing

solu

tion

for h

isto

logy

. Col

lage

n an

d m

uscl

e fib

er s

tain

diff

eren

tially

w

hen

treat

ed s

eque

ntia

lly w

ith A

cid

Fuch

sin,

PTA

/PM

A an

d A

nilin

e B

lue.

Rea

gent

s:

A- W

eige

rt’s

Hae

mat

oxyl

in s

olut

ion

AC

- Aci

d fu

chsi

n st

aini

ng s

olut

ion

E- P

hosp

hotu

ngst

ic a

cid

solu

tion

G- A

cetic

aci

d so

lutio

n

B- W

eige

rt’s

Hae

mat

oxyl

in s

olut

ion

BD

- Pho

spho

mol

ybdi

c ac

id s

olut

ion

F- M

asso

n’s

anili

ne b

lue

solu

tion

Mas

son

trich

rom

e st

anda

rd -

uter

us

34

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MA

SSO

N T

RIC

HR

OM

E G

OLD

NER

Mas

son

trich

rom

e G

oldn

er -

uter

us

num

ber o

f tes

ts10

0pr

oced

ure

time

~40

min

. (+/

- pre

-fixa

tion

time)

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

- 25

°C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

S

tain

ing

solu

tion

for h

isto

logy

. Col

lage

n an

d m

uscl

e fib

er s

tain

diff

eren

tially

w

hen

treat

ed s

eque

ntia

lly w

ith A

cid

Fuch

sin,

PTA

and

Lig

ht g

reen

.

Rea

gent

s:

A- W

eige

rt’s

Hae

mat

oxyl

in s

olut

ion

AC

- Aci

d fu

chsi

n st

aini

ng s

olut

ion

E- L

ight

gre

en s

tain

ing

solu

tion

Nuc

lei

Bla

ckM

uscl

e fib

ers,

cyt

opla

sm, e

ryth

rocy

tes

Red

Col

lage

nG

reen

ish

B- W

eige

rt’s

Hae

mat

oxyl

in s

olut

ion

BD

- Pho

spho

tung

stic

aci

d so

lutio

n

35

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MAY

GR

UN

WA

LD S

TAIN

num

ber o

f tes

ts10

0pr

oced

ure

time

~2 m

in. (

+ G

iem

sa c

o-st

aini

ng)

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

S

uppl

emen

tary

sta

in fo

r May

Gru

nwal

d –

Gie

msa

blo

od s

mea

r exa

min

atio

n.

Rea

gent

s:

May

- G

runw

ald

Sta

in s

olut

ion

Whi

te b

lood

cel

ls

Fain

tly s

hape

dE

ryth

rocy

tes

Pin

kish

-red

May

Gru

nwal

d st

ain

- blo

od s

mea

r

May

Gru

nwal

d st

ain

only

May

Gru

nwal

d - G

iem

sa

36

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MAY

GR

UN

WA

LD -

GIE

MSA

num

ber o

f tes

ts10

0pr

oced

ure

time

~10-

35 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

B

lood

sm

ear e

xam

inat

ion

and

cyto

diag

nosi

s.

Rea

gent

s:

A –

Met

hano

l abs

olut

eC

– G

iem

sa s

tain

ing

solu

tion

Lym

phoc

ytes

Ligh

t blu

e cy

topl

asm

Mon

ocyt

esU

sha

ped

nucl

eus

Gra

nulo

cyte

sN

eutro

phils

(3-6

lobe

d nu

cleu

s), e

osin

ophi

ls (2

lobe

d nu

-cl

eus)

, bas

ophi

ls (w

ith d

ark

viol

et to

bla

ck g

ranu

les)

Pla

tele

tsVi

olet

Erit

rocy

tes

Red

dish

bro

wn

Blo

od p

aras

ites

Red

B –

May

-Gru

nwal

d st

aini

ng s

olut

ion

D –

Sor

enso

n bu

ffer p

H7

May

Gru

nwal

d G

iem

sa -

bloo

d sm

ear

37

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MET

HEN

AM

INE

SILV

ER IM

PREG

NAT

ION

- P.

A.S

.M.

P.A

.S.M

. - k

idne

y

num

ber o

f tes

ts10

0pr

oced

ure

time

~2 h

.pr

oduc

t val

idity

1 ye

ar (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

4 °C

, in

dark

gla

ss b

ottle

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht,

tight

ly c

lose

d

K

it fo

r his

tolo

gy (l

amin

a ba

sala

e, a

rgyr

ophi

l cel

ls e

tc.).

Rea

gent

s:

А –

Per

iodi

c ac

id s

olut

ion

C –

Hex

amet

hyle

ne te

traam

ine

solu

tion

E –

Gol

d ch

lorid

e so

lutio

n

Lam

ina

basa

lae

Bla

ckN

ucle

i and

bac

kgro

und

Pin

kish

-red

and

gre

en if

con

tra-s

tain

edP

ositi

ve c

ontro

lS

kin,

kyd

ney

В –

Silv

er n

itrat

e so

lutio

nD

– S

odiu

m te

trabo

rate

sol

utio

nF

– Fi

xativ

e

38

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

MET

HYL

ENE

BLU

E LO

FFLE

R

Met

hyle

ne B

lue

Loffl

er: A

) mou

th e

pith

el; B

)blo

od s

mea

r

num

ber o

f tes

ts10

0pr

oced

ure

time

~5 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15-2

5 °C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

A

lkal

ine

stai

n fo

r cel

l nuc

lei i

n sm

ears

from

diff

eren

t orig

in. P

atho

gens

(bac

te-

ria) e

xam

inat

ion.

Rea

gent

s:

Met

hyle

ne B

lue

solu

tion

acco

rdin

g Lo

ffler

Bac

teria

B

lue

Leuc

ocyt

es n

ucle

i B

lue

Epi

thel

ial c

ells

cyt

opla

smS

light

ly b

lue

Epi

thel

ial c

ells

nuc

lei

Blu

e

AB

39

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

NEW

MET

HYL

ENE

BLU

E

New

Met

hyle

ne B

lue

- blo

od s

mea

r

num

ber o

f tes

ts10

0pr

oced

ure

time

~20

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

B

lood

sm

ears

exa

min

atio

n fo

r ret

icul

ocyt

es. R

etic

uloc

ytes

are

juve

nile

red

cells

, whi

ch c

onta

in re

mna

nts

of b

asop

hilic

ribo

nucl

eopr

otei

ns. T

his

mat

eria

l rea

cts

with

the

dye

to fo

rm a

blu

ish

blac

k pr

ecip

itate

of g

ranu

les

or fi

lam

ents

.

Rea

gent

s:

New

Met

hyle

ne B

lue

wat

er, i

soto

nic

solu

tion,

tille

d w

ith a

ntic

oagu

lant

Ret

icul

ocyt

esG

reen

ish

to b

lue

with

dar

k bl

ue

ribon

ucle

opro

tein

gra

nule

s in

side

Ery

thro

cyte

sG

reen

ish

to b

lue

40

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

OIL

RED

O

Oil

red

O -

omen

tum

cry

osta

te s

ectio

n

num

ber o

f tes

ts10

0pr

oced

ure

time

~40

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

Li

pid

stai

ning

in fr

ozen

sec

tions

.

Rea

gent

s:

A - P

ropy

lene

gly

col a

bsol

ute

C -

Pro

pyle

ne g

lyco

l 85%

wat

er s

olut

ion

Nuc

lei

Blu

e to

bla

ckLi

pids

RedB –

Sta

inin

g so

lutio

n O

il re

d O

D –

May

er’s

hae

mat

oxyl

in s

olut

ion

41

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

OR

CEI

N fo

r ela

stic

fibe

rs

num

ber o

f tes

ts10

0pr

oced

ure

time

~30

min

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

Ela

stic

fibe

rs

Pur

plis

h re

d-br

own

Bac

kgro

und

Alm

ost c

olou

rless

To

dem

onst

rate

ela

stic

fibe

rs in

tiss

ue s

ectio

ns; r

ecom

men

ded

for v

ascu

lar

path

olog

y.

Rea

gent

s:

А –

Pot

assi

um p

erm

anga

nate

sol

utio

nС

– O

xalic

aci

d so

lutio

nЕ

– O

rcei

n so

lutio

n ac

cord

ing

Shi

kata

В –

Aci

d ac

tivat

ion

buffe

rD

– In

cuba

tion

med

iaF

– D

iffer

entia

tion

solu

tion

Orc

ein

for e

last

ic fi

bers

: A) b

lood

ves

sels

; B) k

idne

yA

B

42

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

PAPA

NIC

OLA

U

num

ber o

f tes

ts10

0pr

oced

ure

time

~25

min

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15 -

25 °

C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

S

tain

sol

utio

n fo

r cyt

o PA

P sm

ears

.

N.B

! EA

65 (E

mm

onya

Bio

tech

Ltd

.) is

an

alte

rnat

ive

for E

A50

sta

in, p

ro-

vidi

ng m

ore

inte

nsiv

e cy

topl

asm

sta

inin

g!

Rea

gent

s:

А –

Har

ris`s

hae

mat

oxyl

in s

olut

ion

C –

EA

50/6

5 po

lych

rom

ic s

tain

sol

utio

nВ

– O

rang

e G

-6 s

tain

sol

utio

n

Pap

anic

olau

for c

yto

PAP

smea

rs: A

) EA

50; B

)EA

65

Nuc

lei

Ora

nge-

brow

n to

blu

ish-

blac

kC

ytop

lasm

(ker

atin

ized

)O

rang

e-pi

nkC

ytop

lasm

(bas

ophi

lic)

Blu

e-gr

een

(EA

50) t

o tu

rquo

ise

(EA

65)

Cyt

opla

sm (m

etap

lasi

a ce

lls)

With

bot

h bl

ue a

nd p

ink

Cyt

opla

sm (e

osin

ophi

lic)

Pin

kE

ritro

cyte

sR

edM

icro

orga

nism

sB

lue-

viol

et to

blu

e-bl

ack

AB

43

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

PER

IOD

IC A

CID

SC

HIF

F (P

.A.S

.)

PAS

reac

tion

- gas

tric

tissu

e (s

tom

ach)

num

ber o

f tes

ts10

0pr

oced

ure

time

~35

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C,in

dar

k gl

ass

bottl

e, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

E

xam

inat

ion

of m

ucos

ubst

ance

s (m

ucop

olys

acch

arid

es),

lam

ina

basa

lae,

gl

ycog

en d

epos

its in

live

r etc

. in

tissu

e se

ctio

ns.

Rea

gent

s:

A –

Per

iodi

c ac

id s

olut

ion

C –

May

er’s

hae

mat

oxyl

in s

olut

ionn

Gly

coge

n, fu

ngi,

neut

ral m

ucos

ubst

anc-

es, a

nd s

ome

lam

ina

basa

lae

Mag

enta

Nuc

lei

Blu

e

B –

Sch

iff R

eage

nt

44

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

RA

PIH

EM

num

ber o

f tes

ts10

0pr

oced

ure

time

~ 40

sec

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

- 25

°C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

Cel

lsC

lear

sha

ped

mor

phol

ogy

(nuc

leus

, cy

topl

asm

), du

e to

spe

cim

en o

rigin

! En-

ablin

g in

terp

reta

tion

of th

eir m

etha

bolit

ic

cond

ition

B

lood

sm

ears

, oth

er c

ytol

ogic

al s

peci

men

s an

d fin

e ne

edle

asp

iratio

ns e

xam

i-na

tion.

R

ecom

men

dabl

e fo

r fas

t blo

od s

mea

rs e

xam

inat

ion!

Rea

gent

s:

A –

Rap

ihem

fixa

tive

С –

Rap

ihem

sol

utio

n II

В –

Rap

ihem

sol

utio

n I

D –

Rap

ihem

was

hing

sol

utio

n -

conc

entra

te

Rap

ihem

: A

) Fin

e ne

edle

asp

iratio

n; B

) Blo

od s

mea

rA

B

45

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

SUD

AN

III -

alc

ohol

sol

utio

n

Sud

an II

I alc

ohol

sol

utio

n - o

men

tum

cry

osta

te s

ectio

n

num

ber o

f tes

ts10

0pr

oced

ure

time

~35

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

S

tain

ing

of tr

igly

cerid

es in

froz

en ti

ssue

sec

tions

.

Rea

gent

s:

A –

50%

Eth

anol

C –

May

er’s

hae

mat

oxyl

in s

olut

ion

Nuc

lei

Blu

e to

bla

ckLi

pids

Ora

nge

B –

Sta

inin

g so

lutio

n S

udan

III (

alco

holic

so

lutio

n)

46

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

SUD

AN

III -

Pro

pyle

ne G

lyco

l

Sud

an II

I Pro

pyle

ne G

lyco

l - o

men

tum

cry

osta

te s

ectio

n

num

ber o

f tes

ts10

0pr

oced

ure

time

~25

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

Tr

igly

cerid

es s

tain

ing

in fr

ozen

sec

tions

.

Rea

gent

s:

A –

Sta

inin

g so

lutio

n S

udan

III

C -

50%

Pro

pyle

ne g

lyco

l wat

er s

olut

ion

Nuc

lei

Blu

e to

bla

ckLi

pids

Ora

nge

B -

85%

Pro

pyle

ne g

lyco

l wat

er s

olut

ion

D –

May

er’s

hae

mat

oxyl

in s

olut

ion

47

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

TOLU

IDIN

E B

LUE

Tolu

idin

e B

lue

stai

ning

: A) s

kin;

B) u

teru

s

num

ber o

f tes

ts10

0pr

oced

ure

time

~6 m

in.

prod

uct v

alid

ity2

year

s (u

nder

reco

men

ded

cond

ition

s)st

orag

e co

nditi

ons

15-2

5 °C

, pro

tect

ed fr

om fi

re a

nd s

unlig

ht, t

ight

ly c

lose

d

1.

To

dem

onst

rate

muc

opol

ysac

char

ides

and

oth

er m

etac

hrom

atic

sub

stan

ces

in ti

ssue

sec

tions

. 2. T

o de

mon

stra

te m

ast c

ells

(with

his

tam

ine,

hep

arin

met

achr

omat

ic

gran

ules

) in

tissu

e se

ctio

ns.

Rea

gent

s:

Tolu

idin

e bl

ue s

olut

ion

Tiss

ue s

truct

ure

Visi

ble

like

in s

tand

ard

Hae

mat

oxyl

in/

Eos

in h

isto

logy

sta

inin

g, b

ut in

blu

e sc

ale.

Mas

t cel

lsVi

olet

met

achr

omat

ic s

tain

ing

Pos

itive

con

trol

Ski

n

AB

48

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

VAN

GIE

SON

TR

ICH

RO

ME

Van

Gie

son

trich

rom

e st

aini

ng: A

) ski

n; B

) pla

cent

a

num

ber o

f tes

ts10

0pr

oced

ure

time

~35

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

S

tain

ing

of c

onne

ctiv

e tis

sue.

Rea

gent

s:

A –

Wei

gert’

s H

aem

atox

ylin

sol

utio

n B

C –

Van

Gie

son’

s pi

crof

uchs

in

Nuc

lei

Bla

ckC

olla

gen

fiber

s R

edM

uscl

e, c

orni

fied

epith

eliu

m, e

ryth

rocy

tes

Yello

w

B –

Wei

gert’

s H

aem

atox

ylin

sol

utio

n B

AB

49

Full

inst

ruct

ions

for u

se o

n w

ww

.em

mon

ya.c

omC

opyr

ight

Em

mon

ya B

iote

ch L

td. ©

WEI

GER

T fo

r ela

stic

fibe

rs

Wei

gert

for e

last

ic fi

bers

- ki

dney

num

ber o

f tes

ts10

0pr

oced

ure

time

~50

min

.pr

oduc

t val

idity

2 ye

ars

(und

er re

com

ende

d co

nditi

ons)

stor

age

cond

ition

s15

-25

°C, p

rote

cted

from

fire

and

sun

light

, tig

htly

clo

sed

To

dem

onst

rate

ela

stic

fibe

rs in

tiss

ue s

ectio

ns; r

ecom

men

ded

for v

ascu

lar

path

olog

y.

Rea

gent

s:

А –

Per

iodi

c ac

id s

olut

ion

С –

Wei

gert’

s re

sorc

in fu

chsi

n so

utio

nЕ

– C

arm

alum

acc

ordi

ng to

May

er

solu

tion

Ela

stic

fibe

rs

Pur

plis

h re

d-br

own

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52

4. Reagents I

Method Reagent StabilityALCIAN BLUE pH 1- PAS A – Alcian blue staining solution рН 1 2

B – Periodic acid solution 2C – Schiff Reagent 2

ALCIAN BLUE pH 2.5– PAS A – Alcian blue staining solution рН 2,5 2B – Periodic acid solution 2C – Schiff Reagent 2

ALCIAN BLUE pH 2.5 A – Alcian blue staining solution рН 2,5 2B – Nuclear Fast Red staining solution 2

ALCIAN BLUE pH 1 A – Alcian blue staining solution рН 1 2B – Nuclear Fast Red staining solution 2

ALCIAN YELLOW – TOLUIDINE BLUE

А – Periodic acid solution 2В – Sodium bisulfite solution 2С – Alcian yellow staining solution 2D – Toluidine blue staining solution 2Е – Sodium hydroxide solution 2

AZAN TRICHROMЕ А – Azocarmine solution 2В – Aniline solution 2C – Differentiation solution 2


53

D – Phosphomolybdic acid solution 2E – Staining solution 2

BRILLIANT CRESYL BLUE Brilliant Cresyl Blue physiological solution 2BENNHOLD’S CONGO RED A – Congo red staining solution according

Benhold2

B – Sodium hydroxide solution 2C – Mayer’s haematoxylin 2

HIGHMAN’ S CONGO RED A- Congo red staining solution according Highman

2

B- Potassium hydroxide solution 2CYTOMAX А – Harris`s haematoxylin solution 2

B – 2-Propanol Absolute 2C – Modified polychromic EA65 solution 2D – 80% 2-Propanol 2

EOSIN – ACQUEOUS SOLUTION 1%

Eosin Y 1% water solution 2

EOSIN – ACQUEOUS SOLUTION 5%

Eosin Y 5% water solution 2

EOSIN – ALCOHOLIC SOLUTION 0.5%

Eosin Y 0.5% alcoholic solution 2

EOSIN-PHLOXINE ALCOHOLIC SOLUTION

А – Mayer’s haematoxylin 2В – Scott’ s solution (water) 2С – Eosin-Phloxine staining solution 2

GIEMSA STAIN Giemsa concentrate solution 2GILL’S I HAEMATOXYLIN Gill’s 1 Haematoxylin solution 2


54

GILL’S II HAEMATOXYLIN Gill’s 2 Haematoxylin solution 2GILL’S III HAEMATOXYLIN Gill’s 3 Haematoxylin solution 2GOMORI SILVER IMPREGNATION

А – Potassium permanganate solution 2В – Acid activating buffer 2С – Oxalic acid solution 2D – Ferric ammonium sulfate solution (alum) 2E – Ammoniacal silver solution 1F – Neutral formalin solution 2G – Sodium hyposulphite solution 2

GRAM STAIN A – Crystal Violet solution 2B – Lugol’s solution for Gram 2C – Decolorising solution 2D – Safranin O alcoholic solution 2

GROCOTT’S А – Chromic acid solution 2В – Sodium hydrogensulfite solution 2С – Methenamine solution 2D – Silver nitrate solution 2E – Borax water solution 2F – Gold chloride water solution 2G – Sodium thiosulfate solution 2H – Light Green solution 2

HAEMATOCOLOR A – Methanol absolute 2B – Haematocolor solution I 2


55

C – Haematocolor solution II 2D - Haematocolor Phosphate buffer 2

HARRIS’S HAEMATOXYLIN Harris’s haematoxylin solution 2LEISHMAN STAIN A – Leishman Staining solution 2

B – Buffered water 2LEISHMAN- WRIGHT STAIN A – Leishman-Wright staining solution 2

B – Buffered water 2MALLORY TRICHROMЕ А – Acid fuchsin staining solution 2

B – Phosphomolybdic acid solution 2C – Mallory’s staining solution 2

MASSON TRICHROME GOLDNER

A- Weigert’s Haematoxylin solution A 2B- Weigert’s Haematoxylin solution B 2C- Acid fuchsin staining solution 2D- Phosphotungstic acid solution 2E- Light green staining solution 2

MASSON TRICHROME STANDARD

A- Weigert’s Haematoxylin solution A 2B- Weigert’s Haematoxylin solution B 2C- Acid fuchsin staining solution 2D- Phosphomolybdic acid solution 2E- Phosphotungstic acid solution 2F- Masson’s aniline blue solution 2G- Acetic acid solution 2

MAY GRÜNWALD - GIEMSA A – Methanol absolute 2


56

B – May-Grunwald staining solution 2C – Giemsa staining solution 2D – Sorenson (Sorensen) buffer pH7 2

MAYER’S HAEMATOXYLIN Мayer’s Haematoxylin solution 2MAY - GRUNWALD STAIN May - Grunwald Stain solution 2METHENAMINE SILVER IMPREGNATION – P.A.S.M

А – Periodic acid solution 2В – Silver nitrate solution 1C – Hexamethylene tetraamine solution 1D – Sodium tetraborate solution 2E – Gold chloride solution 1F – Fixative 2

METHYLENE BLUE LӦFFLER Methylene Blue solution according LofflerNEW METHYLENE BLUE New Methylene Blue water, isotonic solution,

tilled with anticoagulantOIL RED O A – Propylene glycol absolute

B – Staining solution Oil red OC – Propylene glycol 85% water solutionD – Mayer’s haematoxylin solution

ORCEIN А – Potassium permanganate solutionВ – Acid activation bufferС – Oxalic acid solutionD – Incubation mediaЕ – Orcein solution according ShikataF – Differentiation solution


57

PAPANICOLAU А – Harris`s haematoxylin solutionВ – Orange G-6 stain solutionC – EA50 polychromic stain solutionC – EA65 polychromic stain solution

PERIODOC ACID SCHIFF (PAS)

A – Periodic acid solutionB – Schiff ReagentC – Mayer’s haematoxylin solution

RAPIHEM A – Rapihem fixativeВ – Rapihem solution IС – Rapihem solution IID – Rapihem washing solution - concentrate

SUDAN III PROPYLENE GLYCOL

A – Staining solution Sudan IIIB – 85% Propylene glycol water solutionC - 50% Propylene glycol water solutionD – Mayer’s haematoxylin solution

SUDAN III ALCOHOL SOLUTION

A – 50% EthanolB – Staining solution Sudan III (alcoholic solution)C – Mayer’s haematoxylin solution

TOLUIDINE BLUE STAIN Toluidine blue solutionVAN GIESON TRICHROME STAIN

A – Weigert’s Haematoxylin solution AB – Weigert’s Haematoxylin solution BC – Van Gieson’s picrofuchsin.

WEIGERT for elastic fibers А – Periodic acid solution


58

В - Alcoholic reagent for incubation boxС – Weigert's resorcin fuchsinD – Acid differentiation bufferЕ – Carmalum according to Mayer

WEIGERT’S HAEMATOXYLIN A – Weigert’s Haematoxylin solution AB – Weigert’s Haematoxylin solution B

WRIGHT’S STAIN for cytology A – Methanol absoluteВ – Wrght’s staining solutionС – Concentrated Phosphate buffer рН 6,8

COLD ZIEHL-NEELSEN A - Kinyoun’s carbol-fuchsin solutionB - Acetic alcohol solutionC - Methylene Blue solution


59

4. Reagents II

Products Code Size DescriptionAcid ethanol Shikata 155802125E 125ml Differentiating agent

155802250E 250ml155802500E 500ml

Acid ethanol Weigert 153812125C 125ml Differentiating agent153812250C 250ml153812500C 500ml153812100C 1l

Hidrocloric Acid (1M) 1090570125 125ml Differenting agent1090570250 250ml1090570500 500ml1090570100 1l

Kovach’s indole reagent 119002125 125ml For identification of indole119002250 250ml119002500 500ml119002100 1l

Orcein Acetic 1558011125 125ml For chromosomal staining


60

Oxalic Acid 1099650125 125ml Decolorant1099650250 250ml1099650500 500ml1099650100 1l

Phosphate buffer pH 6.8 113273125 125ml113273250 250ml113273500 500ml113273100 1l

Phosphate buffer pH 7.2 111316125D 125ml111316250D 250ml111316500D 500ml111316100D 1l

Picric Acid aqueous sol. 1.2% 110901125 125ml For fixatives mixtures110901250 250ml110901500 500ml110901100 1l

Picric Acid alcoholic sol. 110902125 125ml For erythrocytes in trichrome staining110902250 250ml110902500 500ml110902100 1l

Picrofuchsin 1159270125 125ml For cytoplasm, muscle and collagen


61

Potassium Methabisulfite 0.5% 105057125 125ml Differentiating agent105057250 250ml105057500 500ml105057100 1l

Sodium Thiosulphate 5% 109950125 125ml Decolorant after treatment with lugol solution109950250 250ml

109950500 500ml109950100 1l

Sulphuric Acid 0.5% 109981125 125ml For oxidation with permanganate109981250 250ml109981500 500ml


62

Product Supplementary Code DescriptionDEK – HL DEK-HL decalcifying solution 111300910 For fibrillar and

lamellar bone structureAcid solution for DEK-HL decalcifying solution 111300910.1

Ammonium solution for DEK-HL decalcifying solution 111300910.2DEK – HN DEK-HN decalcifying solution 111300510 For bone tissue

Sodium solution for DEK-HN decalcifying solution 111300510.1DEK – MF DEK-MF decalcifying solution 111300410 Universal

6. Decalcifying solutions

Products Size Code DescriptionBouin 500ml 11400500 Picric acid/formalin

fixative1000ml 1140010002500ml 114002500

Saccomano 25ml 199001025 Fixative for cytology speciments1000ml 199001000

7. Fixatives for histology


63

Products Size Code DescriptionFormaline 38-40% 1L 10561000

5L 10561000Formaline 30% w/v 5L 10561000

1L 10561000Formalin 10% solution pH7.2-7.4 buffered

1L 105710005L 105755000

8. Formalins

Products Size Code DescriptionBio Clear 1L 10271000

5L 10275000Prohyol 95 1L 10075000

5L 10071000Prohyol absolute 1L 10775000

5L 10771000

9. Solvents


64

Products Size Code DescriptionParaffin wax histology 56-58°C 1kg 313110 Lepka 100ml 059801Eurokit 100ml 0405003Canada balsam 100ml 157160598

10. Mounting and embed media


65

• Acute toxicity (oral, dermal, inhalation), category 4• Skin irritation, categories 2, 3• Eye irritation, category 2A• Skin sensitization, category 1• Specific target organ toxicity following single exposure, category 3 o Respiratory tract irritation o Narcotic effects

Acute toxicity (oral, dermal, inhalation), categories 1, 2, 3

• Flammable gases, category 1 • Flammable aerosols, categories 1, 2 • Flammable liquids, categories 1, 2, 3 • Flammable solids, categories 1, 2

• Skin corrosion, categories 1A, 1B, 1C• Serious eye damage, category 1

11. Legend

emmonya biotech catalog

Business

copyright emmonya biotech

staining solutions

eosinphloxin b alcoholic

microbiology emmonya

harris hematoxylin

mayers hematoxylin

weigerts hematoxylin

tissue sections162802250