emerging science on lutein in the brain elizabeth j. johnson, ph.d. jean mayer usda human nutrition...
DESCRIPTION
Structure of the eye adapted from Sung C and Chuang. The cell biology of vision. J Cell Biol 2010;190: Presence of lutein and zeaxanthin in the neural retina Macula Purves D, Augustine GJ, Fitzpatrick D et al., editors. The Retina. Neuroscience 2 nd edition, Sunderland (MA): Sinauer Associates, 2001TRANSCRIPT
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Emerging Science on Lutein in the Brain
Elizabeth J. Johnson, Ph.D.
Jean Mayer USDA Human Nutrition Research Center on AgingTufts University
Boston, Massachusetts USA
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A Consideration for a Role for Lutein in Early Life.
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Structure of the eye adapted from Sung C and Chuang. The cell biology of vision. J Cell Biol 2010;190:953-963
Presence of lutein and zeaxanthin in the neural retina
Macula
Purves D, Augustine GJ, Fitzpatrick D et al., editors. The Retina. Neuroscience 2nd edition, Sunderland (MA): Sinauer Associates, 2001
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Lutein and Zeaxanthin
Lutein and Zeaxanthin
Lutein and Zeaxanthin
Adapted from Purves D, Augustine GJ, Fitzpatrick D et al., editors. The Retina. Neuroscience 2nd edition, Sunderland (MA): Sinauer Associates, 2001
Presence of lutein and zeaxanthin in the neural retina
Lutein and Zeaxanthin
Lutein and zeaxanthin
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Carotenoid concentrations in Serum and Brain
Serum, nmol/L Brain, pmol/g
(mean + se, n=48)
Xanthophylls
Carotenes
a a
aSignificantly greater than other carotenoids
Lutein ZeaxanthinCryptoxanthinb-Carotene Lycopene0
100
200
300
400
500
Lutein Zeaxanthin Cryptoxanthin b-Carotene Lycopene0
20
40
60
80
100
120
Johnson EJ et al. Abstract FASEB J 2011;25:975.21
Cryptoxanthin is the sum of α- and β-cryptoxanthin
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Correlations - serum carotenoids/tocopherol and cognitive function measures
Lutein Zeaxanthin β-carotene α-tocopherol
MMSE (n=305) 0.232p=0.001
0.160p=0.007
0.005p=0.947
0.047p=0.565
BDS (n=305) 0.214p=0.001
0.146p=0.014
0.034p=0.677
0.071p=0.380
FOME Recall (n=305) 0.237p=0.001
0.162p=0.006
0.106p=0.188
-0.012p=0.884
Values are age, sex, education, BMI, diabetes and hypertension adjusted partial correlation coefficients ‘r’ . Significant relations are shown in yellow.
Verbal fluency (n=37) 0.355p=0.075
0.346p=0.084
0.409p=0.146
0.547p=0.043
Word list learning (n=34) 0.306p=0.202
0.241p=0.320
0.470p=0.145
0.561p=0.073
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Correlations - brain lutein, zeaxanthin, β-carotene and α-tocopherol concentrations and pre-mortem cognitive
function measures
MMSE BDS Verbal
Fluency Word List Learning
Lutein 0.638 p=0.035
0.704 p=0.016
0.577 p=0.063
0.542 p=0.085
Zeaxanthin 0.437 p=0.143
0.149 p=0.663
0.495 p=0.121
0.127 p=0.710
β-carotene 0.380 p=0.249
0.178 p=0.600
0.489 p=0.127
0.200 p=0.556
α-tocopherol 0.195 p=0.566
0.030 p=0.930
0.183 p=0.590
0.171 p=0.615
Values are age, sex, education, diabetes and hypertension adjusted partial correlation coefficients ‘r’
n=16
Johnson EJ et al. Abstract FASEB J 2011;25:975.21
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Study design: randomized, double-blinded, placebo-controlled, intervention trial
Subjects: Healthy women (60-80 years)
Intervention (4 months): Placebo (n = 10) Lutein, 12 mg/d (n = 11)
DHA, 800 mg/d (n = 14) Lutein + DHA (n = 14)
Johnson et al. J Nutr Neuroscience 2008;11(2):75-83
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Executive FunctionVerbal Fluency (more = better)
50
70
90
110
130
Placebo DHA Lutein Lutein+DHA
** *
*significantly increased from baseline
Johnson et al. J Nutr Neuroscience 2008;11(2):75-83
% o
f bas
elin
e
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Learning(Shopping List, change in the number of trials to learn list, less =
better)
*
*significantly decreased from baseline
50
55
60
65
70
75
80
85
90
95
100
P l ac ebo DHA Lutei n Lutei n+DHA
*% o
f bas
elin
e
Johnson et al. J Nutr Neuroscience 2008;11(2):75-83
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Short-Term Memory(Memory in Reality Apartment test, more = better)
*significantly increased from baseline
50
60
70
80
90
100
110
Placebo DHA Lutein Lutein+DHA
*
% o
f bas
elin
e
Johnson et al. J Nutr Neuroscience 2008;11(2):75-83
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Lutein in Early Life
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Lutein and Zeaxanthin intake (mg/d) differs with Age (NHANES 2003-2004 data)
1-3 4-8 9-13 14-18 19-30 31-50 51-70 71+0
20
40
60
80
100
120
140
160femalemale
1-3 4-8 9-13 14-18 19-30 31-50 51-70 71+0
200
400
600
800
1000
1200
1400
1600
1800femalemale
Lutein Zeaxanthin*
Age, years Age, years
*Note the 10-fold difference in the y-axis scale between lutein and zeaxanthin
Johnson et al. J Am Diet Assoc 2010;110(9):1357-62
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Plasma lutein and zeaxanthin concentrations in breast fed and formula fed infants
Zimmer JP and Hammond BR. Clinical Ophthalmol 2007;1(1):25-35. Original study data presented in Johnson et al. FASEB (abstract) 1995;9:1869
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Lutein in infant formula has lower bioavailability compared to lutein in breast milk
Bettler J et al. Eur J Nutr 2010;49:45-51
Serum lutein concentrations in infants fed either breast milk, control formula and formula supplemented with lutein for 12 weeks
~20 mcg/L
225 mcg/L
120 mcg/L
45 mcg/L
20 mcg/L
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Infants fed formula (without lutein) may be at a greater risk of low lutein status
Possible implications of low lutein status early in lifeLower macular pigment levelsInfluence early visual developmentAffect maturation of retinal pigment epithelial cellsExpose the retina to damage from short wavelength blue light and oxidative stress
(Hammond BR, Jr. Nutr Reviews 2008;66(12):695-702)
Can low lutein status early in life also affect cognitive development?
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Study Objective
Determine carotenoid concentration in infant brain during the first year of life
4 regions of the brain (hippocampus, frontal cortex, auditory cortex and occipital cortex) were analyzed
Vishwanathan et al. Poster at 16th International Symposium on Carotenoids, Poland, July 2011
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Subjects
Subject characteristics (n=30)
Age, in days 113 + 13 (Mean + SEM)
Sex Males 21 (70%)Females 9 (30%)
Race Caucasian 16 (53%)African American 12 (40%)Hispanic 2 (7%)
Cause of death SIDS 15 (50%)Others 15 (50%)(Bronchopneumonia, dehydration, asthma, drowning, hyperthermia, cardiac disorders, complications of prematurity)
Voluntarily donated samples were obtained from the NICHD Brain and Tissue Bank, of otherwise healthy infants who died during the
first year of life from SIDS or other conditions
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Carotenoid concentration in the brain (mean of the 4 regions)
0
10
20
30
40
50
60M
ean
brai
n ca
rote
noid
con
cent
ratio
n (p
mol
/g)
Lutein Zeaxanthin Crypto β-car Lycopene
*
*significantly different from all other carotenoids at p<0.05
No alpha-carotene was detected. Lycopene detected only in 2 decedents
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Diet (NHANES 1988-1994, 2-11 mo)
Dietary intake versus brain uptake (Percentage of total carotenoids)
Lutein Zeaxanthin Cryptoxanthin beta-carotene Lycopene
7%
Brain carotenoids (0-11 mo)
No alpha-carotene
LuteinZeaxanthinCryptoxanthinalpha-carotenebeta-caroteneLycopene
59%16%
43%
28%
12%
13%
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Xanthophylls are more prevalent in breast milk compared to carotenes
Adapted from Khachik F et al. Anal Chem 1997;69:1873-1881
Adapted from Lietz G et al. J Nutr 2006;136:1821-1827
Data suggest that lutein is actively transported into breast milk and not acquired by passive diffusion
Lutein
Zeaxanthin
Cryptoxanthin
alpha-carotene
beta-carotene
Lycopene
Other carotenoids
Breast milk carotenoids (3 lactating women in US) Carotenoids Milk (nmol/g fat)
n=30 1 month postpartum
3 month postpartum
Lutein 7.41 (5.97-9.20)
9.77 (7.75-12.32)
Zeaxanthin 1.04 (0.82-1.31)
1.23 (0.98-1.55)
α-carotene 0.03 (0.00-0.07)
0.02 (0.00-0.05)
β-carotene 0.82 (0.66-1.03)
0.89 (0.69-1.13)
27%
22%
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Brain carotenoid profile in infants and centenarians
Vishwanathan et al. Poster at 16th International Symposium on Carotenoids, Poland, July 2011Johnson EJ et al. Abstract FASEB J
2011;25:975.21
Lutein Zeaxanthin Cryptoxanthin beta-carotene Lycopene Others
Lutein Zeaxanthin Cryptoxanthin beta-carotene Lycopene
55%15%
18%
31%
22%10%
18%
Others: cis lutein isomers (1%), 9 cis beta-carotene (4%) and
unknown (8%)
Infant Brain (n=30)
Centenarian Brain (n=48)
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Conclusions
• Lutein is the predominant carotenoid in infant brain accounting for more than half the concentration of carotenoids– Lutein may be important for early neural development
• A meta-analysis of 11 studies showed breast feeding was associated with significantly higher scores for cognitive development than was formula feeding
(Anderson JW et al. Am J Clin Nutr 1999;70:525-35)
• As infant formula is not routinely supplemented with lutein, further investigation of the impact of lutein intake on neural development is warranted
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Acknowledgements
Tufts HNRCARohini Vishwanathan, Ph.D.Tammy M. Scott, Ph.D.Emily Eggert (Rice University, Houston, TX)
Alessandro Iannaccone, M.S., M.D., Univ. of TN Martha Neuringer, Ph.D. Oregon Science & Health Univ.Leonard Poon, Ph.D., University of Georgia-AthensLisa Renzi, Ph.D., University of Georgia-Athens D. Max Snodderly, Ph.D., University of Texas-AustinWolfgang Schalch, Ph.D., DSM NutritionalsMatthew Kuchan, Ph.D., Abbott Nutrition