effects of an aqueous extract fromphyllantus niruri

8/9/2019 Effects of an Aqueous Extract Fromphyllantus Niruri

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10 Related Studies Jerico O. Julaton IV-Phoenix

EFFECTS OF AN AQUEOUS EXTRACT FROMPHYLLANTUS NIRURION CALCIUM

OXALATE CRYSTALLIZATION IN VITRO

Phyllanthus niruri is a plant used in Brazilian fol !edicine for the treat!ent of urolithiasis. It "as

pre#iously o$ser#ed that P. niruri sho"s no toxicity% potentially increases calculus #oidin& $y stone for!in&

patients and inhi$its the endocytosis of calciu! oxalate '(aOx) crystals $y *+(, cells. In addition% in a rat!odel of urolithiasis it reduced calculus &ro"th. In the present study% "e e#aluated the effect of an aueous

extract of P. niruri on (aOx crystallization in #itro. (aOx precipitation "as induced $y the addition of 0.1 *sodiu! oxalate to unfiltered urine sa!ples fro! istar rats 'n/1) and nor!al hu!ans 'n/1) in the presence

or a$sence of P. niruri extract '0.23 !&4!l of urine). 5he presence of (aOx crystals "as e#aluated i!!ediatelyand 2 h later. In #itro crystallization of hu!an urine produced typical !ono- and dihydrated (aOx crystals% $ut

only a fe" typical (aOx crystals "ere found in rat urine. 5he presence of P. niruri extract did not inhi$it (aOx

precipitation and e#en !ore crystals "ere o$tained% althou&h they "ere si&nificantly s!aller than those in thecontrol urine. (rystal a&&re&ation o$ser#ed 2 h after crystallization "as also inhi$ited $y P. niruri extract. 5he

results sho"ed an inhi$itory effect of P. niruri extract on (aOx crystal &ro"th and a&&re&ation in hu!an urine%

su&&estin& that it !ay interfere "ith the early sta&es of stone for!ation and !ay represent an alternati#e for!of treat!ent and4or pre#ention of urolithiasis.

STUDY ON IN VITRO ANTI-LITHIATIC ACTIVITY OF PHYLLANTHUS NIRURI LINN. LEAVESBY HOMOGENOUS PRECIPITATION AND TURBIDITORY METHOD

Object!e" ,idney stone is one of the !ost pre#alent diseases "orld"ide and calciu! oxalate has $een sho"n

to $e the !ain co!ponent of the

!a6ority of stones for!ed in the urinary syste! of the patients. 5raditional no"led&e on use of !edicinal

plants for curin& chronic diseases is

pro#in& its "orth to !odern society too. In this re&ards% study "as conducted "ith an o$6ecti#e to find out the

role ofPhyllanthus niruri 7inn. lea#es

extracts to inhi$it stone for!ation and dissolution of already exitin& stone in renal syste! usin& in vitro!ethods in the la$oratory

Met#$%&" 7ea#es po"der "as serially extracted in petroleu! ether% ethyl acetate% !ethanol and "ater.In vitro

study "as conducted to assess antiurolithiatic

effect of plant for all the extracts "ith t"o standard dru&s na!ely% 8eeri and (ystone as control. 5"o !ethods%

tur$idity !ethod and

calciu! oxalate dissolution !ethods "ere practiced to access the inhi$ition of stone for!ation and dissolution

of stone crystals respecti#ely.

*icroscopic study "as done for the co!parati#e e#aluation of crystal density and size in each treat!ent intur$idity !ethod.

Re&'(t&9 ater extract of plant lea#es pro#ed its potential statistically eual to the standard dru&% cystone indissol#in& the exitin& calciu! oxalate

crystals. ater extract could dissol#e 3:.; crystals "hile cystone dissol#ed 3.; crystals in in vitro studyand found statistically at par. ater

extract also could inhi$it up to 3


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!ethanol extract &ot second position in anti-lithiatic acti#ity.

C$)c('&$)9 (o!!on !edical practice reco!!ends use of cystone as effecti#e !edicine for pre#entin& as "ell

as treatin& renal stone "hich found

at par "ith the "ater extract of plant lea#es to inhi$it the stone for!ation e#en in its crude for!. Results &uide

us for the further detailed

in#esti&ation and de#elop!ent of ne" dru&s fro! this !edicinal plant.

EVALUATION OF ANTIUROLITHIATIC ACTIVITY OF COMBINATION OF PHYLLANTHUS

NIRURI AND AERVA LANATA IN ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS

Phyllanthus niruri and ?er#a lanata are "idely used for the treat!ent of urolithiasis in follore syste! of

!edicines. 5he present study "as ai!ed to in#esti&ate the protecti#e effect of co!$ination of !ethanolic

extracts of $oth of the plants in renal cell in6ury. @rolithiasis "as induced $y ad!inistration of 0.>3; #4#

ethylene &lycol in drinin& "ater and si!ultaneously the plant extracts "ere &i#en to the urolithiatic rats $oth

alone and co!$ination for


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ANTIUROLITHIATIC AND ANTIOXIDANT ACTIVITY OFMIMUSOPS ELENGION ETHYLENE

GLYCOL-INDUCED UROLITHIASIS IN RATS

Object!e"

5o e#aluate the potential ofMimusops elengiin the treat!ent of renal calculi.

M+te,+(& +)% Met#$%&"

Petroleu! ether% chlorofor!% and alcohol extracts ofMimusops elengi$ar "ere e#aluated for antiurolithiatic

and antioxidant acti#ity in !ale al$ino istar rats. thylene &lycol '0.>3;) in drinin& "ater "as fed to all the&roups 'Croups IIDIE) except nor!al control 'Croup I) for 2 days to induce urolithiasis for curati#e '(R) and

pre#enti#e 'PR) re&i!en. Croups IV% V% and VI ser#ed as (R% and &roups VII% VIII% and IE as PR "ere treated

"ith different extracts ofM. elengi$ar. Croups I% II% and III ser#ed as nor!al control% positi#e control'hyperurolithiatic)% and standard 'cystone >30 !&4&)% respecti#ely. Oxalate% calciu!% and phosphate "ere

!onitored in the urine and idney. Seru! B@8% creatinine% and uric acid "ere also recorded.In vivoantioxidant

para!eters such as lipid peroxidation '*+?)% &lutathione 'CSA)% superoxide dis!utase 'SO+)% and catalase'(?5) "ere also !onitored.

Re&'(t&"

?ll the extracts ofM. elengi"ere safe orally and exhi$ited no &ross $eha#ioral chan&es in the rats. In

hypercalculi ani!als% the oxalate% calciu!% and phosphate excretion &rossly increased. Ao"e#er% the increased

deposition of stone for!in& constituents in the idneys of calculo&enic rats "ere si&nificantly 'PF 0.001)lo"ered $y curati#e and pre#enti#e treat!ent "ith alcohol extract '?l) ofM. elengi. It "as also o$ser#ed that

alcoholic extract ofM. elengiproduced si&nificant 'PF 0.001) decrease in *+?% and increased CSA% SO+% and

(?5. 5hese results confir! that ?l ofM. elengipossess potent antiurolithiatic acti#ity.

C$)c('&$)"

5he results o$tained su&&est potential usefulness of the ?l ofM. elengi$ar as an antiurolithiatic a&ent.

e/$,%&" ?ntiurolithiatic acti#ity% B@8% creatinine%Mimusops elengi

DIMINUTION OF OXALATE INDUCED RENAL TUBULAR EPITHELIAL CELL IN0URY AND

INHIBITION OF CALCIUM OXALATE CRYSTALLIZATION IN VITRO BY AQUEOUS EXTRACT

OF TRIBULUS TERRESTRIS

Purpose9 Recurrence and persistent side effects of present day treat!ent for urolithiasis restrict their use

so an alternate solution% usin& phytotherapy is $ein& sou&ht. 5he present study atte!pted to e#aluate the

antilithiatic properties of 5ri$ulus terrestris co!!only called as G&ohruH "hich is often used in ayur#eda to

treat #arious urinary diseases includin& urolithiasis.

*aterials and *ethods9 5he acti#ity of 5ri$ulus terrestris "as in#esti&ated on nucleation and the &ro"th of thecalciu! oxalate '(aOx) crystals as "ell as on oxalate induced cell in6ury of 8R, 32 renal epithelial cells.

Results9 5ri$ulus terrestris extract exhi$ited a concentration dependent inhi$ition of nucleation and the &ro"th

of (aOx crystals. hen 8R,-32 cells "ere in6ured $y exposure to oxalate for >2 h% 5ri$ulus terrestris extract

pre#ented the in6ury in a dose-dependent !anner. On treat!ent "ith the different concentrations of the plant

the cell #ia$ility increased and lactate dehydro&enase release decreased in a concentration dependent !anner.

(onclusion9 5he current data su&&ests that 5ri$ulus terrestris extract not only has a potential to inhi$it


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nucleation and the &ro"th of the (aOx crystals $ut also has a cytoprotecti#e role. Our results indicate that it

could $e a potential candidate for phytotherapy a&ainst urolithiasis.

ANTIUROLITHIATIC ACTIVITY OF ETHANOLIC ROOT EXTRACT OF SACCHARUM

SPONTANEUM ON GLYCOLIC ACID INDUCED UROLITHIASIS IN RATS

5he effect of the alcoholic extract of Saccharum spontaneum 'Poaceae) a&ainst &lycolic acidinducedurolithiasis urolithiasis in al$ino rats is su!!arizedin this study. 7ithiasis "as induced in rats $y fed

"ith a calculi-producin& diet '(P+9 co!!ercial diet !ixed "ith 0-1; in !ales% and >-:0; in fe!ales. It causes serious healthpro$le!s such as se#ere pain% urinary-tract o$struction and infection that ad#ersely affect "ell-$ein& of

indi#iduals. 5reat!ent option includes shoc "a#e lithotripsy 'S7)% ureteroscopy% percutaneous

nephrostolithoto!y 'P(87)% and open or laproscopic stone re!o#al "hich are costly and painful. *anysynthetic dru&s lie diuretics and narcotic anal&esics are $ein& used in treat!ent of idney stone $ut o#eruse of

synthetic dru&s% "hich results in hi&her incidence of ad#erse dru& reactions ha#e !oti#ated hu!ans to return to

nature for safe re!edies. ?s her$s and her$al dru&s ha#e clinically pro#en effects lie i!!uno!odulation%adapto&enic and anti!uta&enic% they play a #ital role in treat!ent of idney stone disease. 8u!$er of !edicinal

plants sho"s antiurolithiatic acti#ity such as ,an&hi 'Abuliton indicum)% (haya 'Aerva lanta)% Bishapa or

purna#a 'Boerhaavia diffusa)% ?6u$a 'Bryophyllum pinnatum)% Cohuru 'Tribulus terrestis)% *aa 'Zea mays)

etc.In the present article% an atte!pt has $een !ade to e!phasize on potent indi&enous her$s used in treat!entof urolithiasis.

INFLUENCE OF CUCUMIS TRIGONUS R. FRUIT EXTRACT ON BIOCHEMICAL PARAMETERS

INUROLITHIASIS INDUCED *ISTAR ALBINO RATS

Plants are utilized as therapeutic a&ents since ti!e i!!e!orial in $oth or&anized '?yur#eda% @nani) and

unor&anized 'fol% tri$al% nati#e) for!. 5he ethanolic fruit extract of (ucu!is tri&onus Rox$ of fa!ily(ucur$itaceae "as used to treat the urolithiasis induced $y ethylene &lycol. On this course% the extract also

repairs the chan&es that happened in the $ioche!ical para!eters urea% uric acid and creatinine in seru! and

urine of the urolithiatic rats. 5he ethanol extract '130 !& 4 & $.".) reduced the le#els of ele#ated $ioche!ical

para!eters in seru! and urine si&nificantly 'pF0.03) "hen co!pared "ith the toxic &roups. 5he results sho"n

$y the ethanol extract '130 !& 4 & $.".) "as co!pared to standard thiazide dru& treated &roup sho"in& no

si&nificant difference 'pF0.03) and thus exhi$ited potent antiurolithiatic acti#ity.


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T*O SYNERGETIC EFFECTS OF SONCHUS ARVENSIS L. LEAVES DECOCTION IN CALCIUM

OXALATE BLADDER STONE THERAPY ON MALE *ISTAR RAT

5he anticalculi effect of Sonchus ar#ensis 7. decoction on rat $ladder stone has $een done. 5he

o$6ecti#e of the research is to deter!ine its effect in $ladder stone therapy. Bladder stone "as done $y !atrix-

&lycolic acid induction !ethod% used sil strin& !atrix "hich inserted $y sur&ical in $ladder and t"o day

after"ards &i#en per orally &lycolic acid at the dosa&e 300 !&4& B4day durin& > days. Pre#enti#e &roup "astreated "ith 1..; 'p0.03) the inhi$ition effect. In

curati#e occurred the inhi$ition and dissol#in& effects of existed $ladder stone :.30; 'p0.02) and .02;

respecti#ely. In conclusion t"o effects can $e sho"n na!ely '1) pre#enti#e !easure9 $y the inhi$ition effect% it

inhi$its the for!ation of $ladder stone and '2) curati#e !easure9 $y the inhi$ition of $ladder stone enlar&e!ent

and dissol#in& of existed $ladder stone.

effects of an aqueous extract fromphyllantus niruri

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