editorial genomics - fr.vwr-cmd.comfr.vwr-cmd.com/ex/downloads/life_science/biomarke/... · vwr...

Promotions for Life Scientists

�� August 2010��

EDITORIAL ISSUE 16 - APRIL 2010

Local price agreements apply.

NO

http://no.vwr.com/lifescience

WWW.VWR.COMEditorVWR International bvbaResearchpark Haasrode 2020Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International

Layout and typesettingMarketing Services VWR

PrintingStork, Germany

No part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe.

Run80.500 copiesPublication date: April 2010

Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.

Genomics

��New TC-PLUS PCR cycler from Techne 1��PCR purification with Omega Bio-Tek 1��New Taq Mastermixes from VWR Collection 1 ��DNA and RNA decontamination from Applichem 1��Dual Bay PCR cycler from VWR Collection and free

oligos from Thermo Biopolymers 2��PCR plates from Thermo Scientific 2 ��Bag in a box mol biol grade water from

BDH Prolabo 2��DNA, RNA and protein extraction in one go from

GE Healthcare 2��Reliable RNA extraction from 5PRIME 3��DNA quantitation from Biotium 3��Easy DNA isolation from ZyGEM 3��Optimise your qPCR reations with Quanta

Biosciences 4��Gel doc system from VWR Collection 4��New horizontal gel tanks from VWR Collection 4��New, unique electroporation buffer from BTX 4

Proteomics

��ChromaLinKTM Biotin labelling kit from SoluLink 5��Dialysis made simple with Spectrum’s

Float-A-Lyzer 5��Easy to use microplate photometer from

�� New vertical electrophoresis tanks from

VWR Collection 6

Cell biology

��New BD PureCoat TM 6��Replacement of transferrin from Biochrom 6��Cayman’s bioactive lipids 7��New 2-D Cryogenic vials from Wheaton 7��Vacucap filtration devices from PALL Life Sciences��ProteaseARRESTTM from G-Biosciences 7

Supporting products

��ART® barrier tips from MBP 8��Laptop coolers from Nalgene 8��CoolCell from Biocision 8

IN THIS ISSUE

��Welcome to the first edition of the VWRbioMarke shop in 2010. We hope that you’re getting lots of spring sunshine.

As usual we have some great new innovations for you such as the new thermal cycler from Techne - a new partner in the programme this year plus some great offers on reagents, kits and other supporting products from our regular partners.

Enjoy this issue and don’t forget to check out online for up to the minute information from VWR.

Techne’s New TC-PLUS Thermal CyclersSpace-saving thermal cyclers….

The TC-PLUS thermal cyclers have all the very latest features such as colour touch screens and fast ramp rates, along with some innovative additions such as the space-saving, stackable design and unique automatic, self-adjusting heated lid (patent pending).

**4 years on unit and 4 years or 100,000 cycles on block, whichever comes first.

500 reaction Taq DNA Polymerase Mastermixes from VWR Collection!Make your PCR set up easy and less expensive! Just add your template and primers.

As requested by many of our happy VWR Collection PCR reagent users, we are now launching a 500 reaction pack size. We are celebrating this by giving you 30% OFF!Also available as RED Taq, where a RED inert dye is included that also acts as a loading dye for electrophoresis.

promo code

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Description Cat. No. Price Promo Price

1.1 x Mastermix Taq DNA polymerase 1.5 mM MgCl2 733-2540P 1125,- 785,-




1.1 x Mastermix RED Taq DNA polymerase 1.5 mM MgCl2 733-2544P 1125,- 785,-




Request a FREE sample by emailing [email protected]

��

mo Price

785,

785,

785,

785,

785,

785,

785,

785,-

ng [email protected]

e

,-

,-

,-

,-

,-

,-

,-

NEW!30% OFF!

NEW

Purchase any TC-PLUS

and get a SCF1 spinner

free!

�� 5 ºC/sec ramp rate with gradients on all blocks

�� TC-PLUS units can control up to 3 satellite units directly

�� Satellite units; controllable by the TC-PLUS or a PC using the free software

�� 4 year warranty*

Genomicspromo

code1

Description Cat. No. Price

TC-PLUS thermal cycler with 96 x 0.2 ml block 732-1271P 55195,-

TC-PLUS thermal cycler with 384 well plate block 732-1273P 55195,-

Erase lab contamination problems now with AppliChem’s ExitusPlus-technology

DNA-ExitusPlus™Reagent for the removal of DNA and RNA contamination

RNase-ExitusPlus™RNase decontamination solution

promo code

3

�� Rapid non-enzymatic, non-sequence-specific degradation

�� Readily bio-degradable and not harmful or toxic

�� No aggressive mineral acids or alkaline substances, no bleach

New formulations with improved activity and less colour indicator!

Big savings!PCR purification kit from Omega Bio-Tek, spin column format.

promo code

2

Description Qty/Pk Cat. No. Price

EZNA CYCLE-PURE kit 200 OMEGD6492-02P 1125,-

Innovations in nucleic acid isolationleic acidleic acid

Buy one get one free!

from

Qt /Pk C t N

Buy onone f

promo

d less colour indicator!

ontaminationation


DNA-ExitusPlus™ spray bottle and FREE refill bottle A7089.1000P 1000,-

RNase-ExitusPlus™ spray bottle and FREE refill bottle A7153.1000P 990,-

Buy a spray bottle

and get a refill bottle

free!

Place your order today! Visit our website http://no.vwr.comLocal price agreements apply.

Offers valid from 12th April to 31st August 2010Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders.2

ISSUE 16 - APRIL 2010��Genomics Simple yet indispensable

Molecular biology grade water with the BDH Prolabo guarantee of quality and performance.

Our fantastic bag in the box packaging means that you can buy in an affordable 10 l pack and be assured that the inner bag will collapse as the product is used keeping every drop as fresh as the first. DNase, RNase and protease free.

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Thermo Scientific PCR PlatesThermo Scientific ABgene PCR plastics are produced in a state of the art facility, using world leading Quality Control/Quality Assurance to deliver plates that give you confidence in your results:

�� Molecular grade virgin polypropylene chosen for its biocompatibility and beneficial properties in PCR applications – low adsorption and maximum sample recovery

�� Mould design ensures ultra thin well walls for optimal heat transfer�� Raised rim design around each well delivers compatibility with any sealing

method

Commitment to quality ManufacturingCleanroom productionFree of particle contamination and certified free of RNase, DNase, Endotoxins and human DNA.

Stringent quality control�� Every plate is visually inspected�� Every well is tested for integrity (no micro holes) �� Every batch sample tested for sealing efficiency and cycled for evaporation

checking

Note: please consult your local VWR sales specialist for compatibility with your instrument

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Description Pack Size Cat. No. Price Promo Price

Water in Box, Molecular Biology Grade 10 l 445847DP 500,- 250,-

illustra™ triplePrep™: DNA, RNA and total denatured proteins from the same undivided sample in 1 hourThe illustra triplePrep Kit is designed for the rapid extraction of genomic DNA, total RNA and total denatured protein from a single undivided sample, from as little as 1 mg of animal tissue or 3 x 105 cultured cells. DNA, RNA and protein can be isolated from a range of tissue types such as liver, kidney, spleen, brain, lung and intestine and cell lines.

�� Correlate DNA/RNA/protein data directly from exact same sample

�� Generate more data from precious limited sample

�� Faster and more flexible workflow�� Higher DNA/RNA/protein yield

from small sample input�� DNase provided in the kit, for

DNA-free RNA �� Functionally tested for purity and

yield

Examples of downstream applications: Genomic DNA: PCR, sequencing, array CGH, restriction enzyme digestion Total RNA: RT-PCR, cDNA synthesis, gene expression arrays Total protein: SDS-PAGE, Western Blotting, 2-D DIGE, LCMS

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VWR Collection - two block functionality at an incredible price

The VWR DOPPIO thermal cycler is an ideal solution for scientists looking to maximise their research funds without compromising flexibility and functionality.

Two independent 48 well blocks, both with sprung heated lids adapt to both tubes and high profile 48 well plates. Two independant programmes can be run or one programme simultaneously on both

blocks.

Want gradients? Who wouldn’t?! The DOPPIO has the ability to run gradient programs as a standard feature. Simply enter a gradient step into your protocol, and the DOPPIO will generate a gradient over 8 columns. If you want a wider gradient range, then simply use both blocks!!

As part of the VWR Thermal Cycler Collection family of the thermal cyclers, the DOPPIO benefits from the same software architecture as the other products in the range. The DOPPIO uses Windows CE as its control platform, with a 3.5” full colour touch screen. Users are able to store up to 1000 programs within the onboard software.

Buy 4

pay for 3

Buy 3 pay for 2

ABgene 96 well plate Cat. No. Price for 4 packs

Promo price for 4 packs

Non-skirted plate 732-4828P 3760,- 2820,-

Skirted plate 732-4888P 3760,- 2820,-

ABgene 96 well plate Cat. No. Price for 3 packs

Promo price for 3 packs

Semi-skirted plate for regular ABI block 732-0892P 3750,- 2500,-

THERMO-FAST ® PCR 96 PLATE 731-0302P 2760,- 1840,-

Description Qty/Pk Cat. No. Price Promo Price

illustra triplePrep (DNA, RNA and protein) 50 preps GEHE28-9425-44P 4320,- 2160,-

promo code

8

50 preps for 50% OFF for the first 50 European

customers!

Order your DOPPIO now,and take advantage of the

introductory offer. Email your machine’s

serial number to [email protected]

and we will send you a free of charge voucher from Thermo

Scientific Biopolymers onoligos to a value of 200 EUR.

The VWR DOPPIO thewithout compromis

Two independen48 well plate

blocks.

Want grastandara gradi

As pabenDOUs


DOPPIO Thermal Cycler with 2 independent working 48 well blocks 732-1210P 29000,-

200 EURO of free oligos


Offers valid from 12th April to 31st August 2010Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders. 3

ISSUE 16 - APRIL 2010��RNA Extraction: decrease costs - increase yields!25% off on Isol-RNA Lysis Reagent, free Phase Lock Gel™ sample

For many samples organic extraction is the method of choice to isolate RNA. Isol-RNA Lysis Reagent is phenol/guanidine based and enables efficient lysis of a variety of cell and tissue types, leading to high yields of RNA.

Try Phase Lock Gel™ tubes to improve this process by creating a stable barrier between organic and aqueous phase. This enables you to simply decant the aqueous phase before precipitating your RNA.

Combine Isol-RNA Lysis Reagent with Phase Lock Gel™ Heavy to get:�� Increased yields by up to 30%�� Higher purity, no interphase contamination�� Reduced contact to toxic organic solvents

Phase Lock Gel is also available for different sample volumes and as lower density gel for selected DNA purification protocols.

promo code

9DNA quantitation kits from Biotium

AccuBlue™ kits are DNA quantitation kits offering tremendous convenience, sensitivity, and wide dynamic range. Key features of AccuBlue™ kits include:

Pack size: 1000 assays.

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Description Contains Cat. No. Promo price

AccuBlue™ High Sensitivity ds Quantitation Kit Quantitation solution, enhancer and DNA standards BTIU31006P 1850,-

AccuBlue™ Broad Range ds Quantitation Kit Quantitation solution, enhancer and DNA standards BTIU31007P 1850,-

AccuBlue™ High Sensitivity ds Quantitation Solution Quantitation solution and enhancer (no DNA standards) BTIU31008P 1250,-

AccuBlue™ Broad Range ds Quantitation Solution Quantitation solution and enhancer (no DNA standards) BTIU31009P 1225,-

Conc

entr

atio

n (u

g/m

L)

Rela

tive

Flur

esce

nce

Accelerate ALL your extractions with prepGEM!

promo code

11

Description Rxn Cat. No. Promo price

prepGEM Tissue 20 ZYGEPTI0020SAMPLE FREE

prepGEM Bacteria 20 ZYGEPBA0020SAMPLE FREE

prepGEM Blood 20 ZYGELBL0020SAMPLE FREE

prepGEM Storage Card Blood 20 ZYGEPSB0020SAMPLE FREE

prepGEM Saliva 20 ZYGEPSA0020SAMPLE FREE

prepGEM Storage Card Saliva 20 ZYGEFSS0020SAMPLE FREE

prepGEM Insect 20 ZYGEPIN0020SAMPLE FREE

AccuBlueTM technology is covered by pending US and international patents.AccuBlueTM is a trademark of Biotium, Inc.

Description Pack size Cat. No. Price Promo Price

Isol-RNA Lysis Reagent 200 ml 733-1089P 2225,- 1660,-

Description No. tubes Cat. No. Price

Phase Lock Gel Heavy 1.5 200 713-2534P 1275,-

Phase Lock Gel Heavy 2 200 713-2536P 1520,-



Request your free Phase Lock Gel Heavy sample pack (four 2 ml tubes) at [email protected]!

Description No. tubes Cat. No. Price

Phase Lock Gel Light 1.5 200 713-2533P 1275,-

Phase Lock Gel Light 2 200 713-2535P 1520,-



Phase Lock Gel in Action

Request a free sample of the solutions: Email [email protected]

Mouse gender typing ref: ZyGEM Mouse app note.

The prepGEM range gives you low cost, rapid and reliable DNA extraction from blood, cell culture, insects, and almost any tissue material. The single, closed tube, temperature controlled protocol will save you time and money, and reduce waste. Published references on automation, and applications such as cancer microbiopsies, single cell analysis, cryptosporidium testings as well as nematode and insect genotyping using WGA, PCR, qPCR are available, please enquire.

are DNA ffering

ience, dynamic

e:

25% off and

request a

Free sample”

25% off Isol-RNA Lysis reagent! and

free Phase Lock Gel(up)TM)

�� High sensitivity: detect as little as 200 pg dsDNA (HS) or 2 ng (BR) per well

�� Wide linear detection range: quantify dsDNA from 0.2 to 100 ng (HS) or 2 to 2000 ng (BR)

�� Safety: high sensitivity kit is cell membrane impermeable to eliminate or reduce any potential genotoxicity

�� Excellent selectivity: minimally affected by RNA or ssDNA and many other common contaminants

�� Superior precision: less than 5% CVs

�� Unmatched stability and convenience: contain a stable 1X aqueous solution, 100X enhancer and dsDNA standards for use at anytime

�� Compatibility: compatible with microplate readers or the hand held Qubit™ fluorometer, or NanoDrop and NanoVue from GE Healthcare

�� Ex/Em: High Sensitivity 485/530 nm; Broad Range 350/460 nm

Call now for a free sample, then send in

your results to techsupport@zygem.

com and go in the draw to win one of ten iPOD Nano each

quarter



ISSUE 16 - APRIL 2010��White PCR plates always improve qPCR performance and sensitivity?

White PCR plates can significantly improve fluorescence signal and enhance real-time qPCR performance and sensitivity. However, visualising and dispensing clear reagent solutions in white plates is often difficult and can lead to undesirable technical errors and failures. Additions of AccuVue Blue Dye to the qPCR reagent produces a light blue colour that enhances visual contrast to minimise pipetting errors. It does not interfere with PCR performance or affect the stability of the SuperMix or FastMix.Standard Cycling - PerfeCTa™ SYBR® Green SuperMix and FAST Cycling - PerfeCTa™ SYBR® GreenFastMixes™ - both are ready-to-use 2x reaction mixes that contain all components, except primers and template.

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*AccuVue Blue Dye, QUNT95090-100 +50 white plates suitable for your instrument of choice, is included.

Genomics

Introduction: NEW horizontal shirogel electrophoresis gel tank from VWR Collection�� Moulded, durable gel box and gel tray�� Rubber gaskets for tapeless gel casting�� “EasyLift” safety lid with buffer recapture system�� Gel dimension: 15 x 10 cm�� Supplied combs: 16 and 28 teeth

250 g DNA Grade Agarose included free of charge

GenoSmart the only gel documentation system you needFor high quality and fast documentation and analysis of your DNA or Protein gels, the VWR Collection is just what you need. Easy-to-use and very versatile, the unit is compatible with most commonly used electrophoresis gel stains and by using a white or blue light converter on your VWR or other brand of transilluminator (not supplied), you can also capture images from many other applications. Transfer your data onto a USB stick and you can work on images on your PC.

promo code

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y p g y pp yto a USB stick and you can work on on your PC.

�� Real-time images from 8-bit CCD camera (768 x 582 pixel resolution)�� Self-contained, microprocessor controlled darkroom�� Integrated colour LCD screen for image viewing�� White Epi-light�� Instant on-screen saturation detection�� Rapid photographic print production


GenoSmart Gel documentation system 730-3000P 45000,-

shirogel VWR Collectiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiionnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn

Description Gel Size Cat. No. Price

shiroGELMidi Plus 10 Electrophoresis unit 15 x 10 cm 700-0253P 2450,-

Free Agarose

BTXpress High performance electroporation solution

Increased transfection efficiencies and cell viability�� High efficiency transfection of cell lines and previously considered “hard

to transfect”cells

Single buffer for all cell types�� One electroporation buffer for all mammalian cell lines

Cost effective�� Multiple kit configurations to meet your demand, more samples per kit

at a lower cost compared to competitors

Control�� Choose your electroporation settings to create the highest transfection

efficiencies to gain control over optimisation

BTX is pleased to introduce the new BTXpress “High Performance Electroporation Solution”. BTXpress greatly increases the transfection efficiencies and cell viabilities in a variety of cell lines and cells considered difficult to transfect. Overcome the challenges associated with transfecting difficult cell lines by combining the performance of the BTXpress electroporation solution with the versatility of the BTX electroporation systems. Request a free sample today to test in your own lab.

BTXpress Solution Kit

promo code

15

ard

30% off andFree sample

f andFree sampleample

Free AccuVue Dye

* l hi l

Real-Time Instrument Cat. No. Price

Bio-Rad iCycler, iQ4/5,MyiQ QUNTVWRI-1P 5780,-

Bio-Rad CFX, Opticon, Chromo 4/5 Eppendorf RealPlex QUNTVWRI-2P 5780,-

ABI 7000 , 7300, 7700, 7900, 7900HT QUNTVWRI-3P 5780,-

ABI 7900HT Fast, StepOne Plus QUNTVWRI-4P 5780,-

ABI 7500 QUNTVWRI-5P 5780,-

ABI 7500 Fast QUNTVWRI-6P 5780,-

Stratagene Mx (any) QUNTVWRI-7P 5780,-

Standard Cycling - PerfeCTa™ SYBR® Green SuperMix *

Real-Time Instrument Cat. No. Price

Bio-Rad iCycler, iQ4/5,MyiQ QUNTVWRI-8P 6340,-

Bio-Rad CFX, Opticon, Chromo 4/5 Eppendorf RealPlex QUNTVWRI-9P 6340,-

ABI 7000, 7300, 7700, 7900, 7900HT QUNTVWRI-10P 6340,-

ABI 7900HT Fast, StepOne Plus QUNTVWRI-11P 6340,-

ABI 7500 QUNTVWRI-12P 6340,-

ABI 7500 Fast QUNTVWRI-13P 6340,-

Stratagene Mx (any) QUNTVWRI-14P 6340,-

FAST Cycling - PerfeCTa™ SYBR® Green FastMixes*

50 White PCR Plates

Free of Charge!

Free 20 x 20 cm 312 nm

transilluminator with every purchase

Includes No of reactions Cat. No. Promo price

5 ml bottle BTXpress solution, 50 x 2 mm gap cuvettes with transfer pipettes 50 732-1280P 1660,-




Includes No of reactions Cat. No. Promo Price

5 ml bottle BTXpress solution 50 732-1284P 1315,-

10 ml bottle BTXpress solution 100 732-1285P 2500,-

Request a free sample of the solutions: Email [email protected]

BTXpress Solutions:



ISSUE 16 - APRIL 2010��Proteomics

Thermo Scientific Multiskan FC - a reliable and easy to use microplate photometer

Thermo Scientific Multiskan FC microplate photometer is designed with 30 years experience in microplate photometry. It is a reliable and robust instrument for a wide variety of photometric research and routine applications offering ease of use via the large display, visual user interface and multiple language options.

Choose from the following filters:*

* Offer is valid for standard filters, worth €168 - €323

promo code

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Spectrum makes Dialysis simplepromo

code17

For more information, please read SPECTRUM’s article of the electro-elution application in the VWRbioMarke MAGAZINE.

735-0400 Optical filter 340 nm 735-0403 Optical filter 414 nm735-0408 Optical filter 492 nm 735-0411 Optical filter 540 nm735-0413 Optical filter 550 nm735-0416 Optical filter 570 nm735-0420 Optical filter 595 nm735-0426 Optical filter 630 nm735-0428 Optical filter 650 nm

Email [email protected] and supply us with the serial number of the instrument you purchased, state the wavelength of the filter you want, and we will send you your free filter.

Float-A-Lyzer® G2Is it protein concentration, desalting, buffer or pH change you need to do? Are you doing binding studies, solute fractionating or electro-elution? The most gentle, easy and inexpensive way is to dialyse your samples with the Spectra/Por® Tubing or much better: with the easy-to-use dialysis device Float-A-Lyzer® G2.

(Daltons) MWCO �� Price

1 ml

100 – 500 734-1494SAMPLE Free of charge

3 500 – 5 000 734-3573SAMPLE Free of charge

8 000 – 10 000 734-3574SAMPLE Free of charge

20 000 734-3575SAMPLE Free of charge



FloIs it to doAre yelutiyoureasy

Free sample

40% off

ChromaLink™ Biotin labelling kitEasy protein and antibody labelling:

promo code

16

promocode

16

Still not convinced? Request a free trial kit at [email protected]

30% off


ChromaLink™ Biotin labelling kit 733-2500P 3,000,- 2,100,-


Multiskan FC microplate photometer (405, 450 and 620 nm filters already included) 736-0355P 63375,- 48000,-

Multiskan FC with incubator (405, 450 and 620 nm filters already included) 736-0356P 87175,- 59000,-

Buy NOW get 30% off...

Still not convinced? Request a free

trial kit at vwrbiomarke@

eu.vwr.com

�� Biotinylate and quantify with 1 reagent�� Determine the exact number of attached biotins with

simple UV scan�� Easy-to-use protocols on USB stick�� Complete kit labels 5 x 1.0 mg of protein or antibody

Purchase a Multiskan FC and get an additional

filter* FREE!

(Daltons) MWCO

Colour code

��

1 ml Price per pack of 12

Promo Price


Promo Price


Promo Price

100 – 500 Green 734-1494P 1525,- 915,- 734-1496P 1700,- 1020,- 734-1498P 1725,- 1035,-

3 500 – 5 000 Black 734-3573P 1600,- 960,- 734-3577P 1775,- 1065,- 734-3581P 1775,- 1065,-

8 000 – 10 000 Yellow 734-3574P 1600,- 960,- 734-3578P 1775,- 1065,- 734-3582P 1775,- 1065,-

20 000 Red 734-3575P 1600,- 960,- 734-3579P 1775,- 1065,- 734-3583P 1775,- 1065,-

50 000 Violet 734-1495P 1450,- 870,- 734-1497P 1600,- 960,- 734-1499P 1625,- 975,-

100 000 Blue 734-3576P 1600,- 960,- 734-3580P 1775,- 1065,- 734-3584P 1775,- 1,065,-



ISSUE 16 - APRIL 2010��

Cell biology

Proteomics

New BD PureCoatTM Cultureware! Line Extension with Flasks.The innovative new surface technology from BD Biosciences is now available on cell culture flasks. It improves significantly the attachment, growth and differentiation of various cell lines and primary cells, in particular fastidious cell types.

To order samples for testing the new surfaces Email [email protected]


75 cm2 flask, vented cap, canted neck 5 734-2212P 515,-

BD PureCoat Amine Cultureware 24 well plate 5 734-1473P 745,-

BD PureCoat Amine Cultureware 100 mm dish 10 734-1481P 790,-


75 cm2 flask, vented cap, canted neck NEW 5 734-2213P 220,-

24 well plate 5 734-1485P 290,-

100 mm dish 10 734-1489P 355,-

BD PureCoat Carboxyl Cultureware

BD PureCoat Amine Cultureware

�� Ideal for culture in low serum or no serum conditions.�� BD PureCoat amine well suited for neuronal Pcell types�� Better freeze-thaw recovery of cryopreserved cells

�� Improved differentiation of cells�� Animal-free �� Available as amine or carboxyl surface chemistries

Replacement for transferrin and proliferation stimulating activity for a large number of cell culture applications.

PROXYFERRIN is a chemically defined low molecular substitute for holo-transferrin supporting the transferrin-independent iron transport into in vitro cultured mammalian cells. PROXYFERRIN’s proprietary composition is free of animal derived components, pyrogens and proteins. The concentrated substitute combines several benefits as negligible biohazard, low costs compared to recombinant transferrin, suitability for many cell types, particularly low iron tolerant cells, endotoxin content below detection limit and stability upon storage. The main application of PROXYFERRIN offers versatility and safety for the design of hormonally defined serum-free cell culture media. Additionally, it can provide improved cell proliferation stimulating activity to low serum supplemented media, as well as to the activity of serum supplements with low iron status.

PROXYFERRIN is ideal for all media that must contain solely chemically defined components of non-animal origin.

Order a free

sample!

Description Pack Size Cat. No. Price

Proxyferrin 15 ml BCHRK3702P 585,-

Proxyferrin 100 ml BCHRK3703P 2600,-

An overview of the advantages:�� Endotoxin tested�� Sterile filtered�� Protein-free�� Cost-effective�� High level of safety�� Cell proliferation stimulating activity

promo code

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BACK IN BLACK... kuroGEL The NEW VWR Collection electrophoresis units Our new ranges of vertical systems incorporate features that result in improved performance through much tighter material tolerances, but are completely compatible with our previous vertical systems.

kuroGEL Verti 10 x 10 cm dual vertical unitsThe kuroGEL Verti 1010 mini-vertical gel units are a dual-gel unit, with all the important features required by research scientists. They are flexible and come either cooled or un-cooled and with a casting base. In addition to self-cast gels, these units can run most 8 x 10 cm or 10 x 10 cm commercially available pre-cast gels.Supplied complete with casting base for 2 10 x 10 cm gels supplied with 1 casting base, 2 plain glass plates, 2 notched glass plates, 1 dummy plate, 2 x 1 mm spacer sets and 2 x 1 mm thick 12 sample combs

promo code

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Description Cat. No. Price Promo price

kuroGEL Verti 1010Y un-cooled 700-0167P 4650,- 2790,-

kuroGEL Verti 1010YK cooled 700-0169P 5775,- 3465,-

kuroGEL Verti 20 x 20 cm dual vertical unitsAll the benefits of the kuroGEL Verti 1010, but in a much larger gel size and with increased sample throughput. A cooling coil within the kuroGEL Verti 2020YK gel tank allows faster separations to be performed at high voltage without compromising resolution. Supplied as above, but in 20 x 20 cm format and 24 sample combs


kuroGEL Verti 2020Y un-cooled 700-0172P 7950,- 4770,-

kuroGEL Verti 2020YK cooled 700-0174P 9100,- 5460,-


kuroGEL Verti 1816K 700-0189P 12775,- 7665,-


kuroGEL Verti 1824K 700-0177P 22325,- 13395,-

kuroGEL Verti 1816K Dual Cooled Vertical UnitThe maximum glass plate size 18 x 16 cm, produces straight lanes and sharp and well defined bands. The kuroGEL Verti 1816K system can run up to four gels (maximum 112 samples) at one time under identical conditions. Gels can run at uniform temperature from 1 to 45 °C and the system can accommodate denaturing and native polyacrylamide gels and 2-D electrophoresis. A wide array of accessories allows you to tailor gel configurations to your needs. Supplied with upper and lower buffer chamber, safety lid with high voltage leads, heat exchanger, 6 18 x 16 cm glass plates, 4 16 cm clamp assemblies, 6 cams, 2 slotted gaskets for upper buffer chamber, dual gel casting stand with levelling base, bubble level, 2 laminated gaskets for caster, buffer dam, spacer-mate alignment template, wonder-wedge plate separation tool, gel seal, 2 x 1.5 mm thick 15 sample combs, 4 x 1.5 mm thick spacers (2cm wide)

kuroGEL Verti 1824K Dual Cooled Vertical UnitEssentially the same as the kuroGEL 1816K unit, this taller format accommodates 24 cm long gels for increased product resolution. Supplied complete as above but with larger 18 x 24 cm plates.

40% Introductory discount off the vertical

units

Choose one from the following

formats (plates, flasks, or dishes) and get your set amine/carboxyl

free!

To order samples for testing the new surfaces Email [email protected]



ISSUE 16 - APRIL 2010��New WHEATON CryoELITE™ cryogenic vials

The Wheaton CryoELITE cryogenic vials are a perfect solution for your precious sample management. Whether you are storing biospecimens in biobanks for genetic research, studying responses for treatment of diseases or managing manual or automatic compound storage facilities, the new CryoELITE Cryogenic Vials are designed to protect the integrity of your irreplaceable samples.

Wheaton offers a full range of external and internal cryogenic vials, free-standing and round bottom styles ranging from 1.2 – 5 ml. Seven different coloured caps are standard on the 2 ml size and they match the colours of the popular Wheaton CryoFile™ Storage Boxes. Caps and vials are made from cryogenic, radiation grade, virgin polypropylene and they are offered sterile or non-sterile.

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Cap Colour Cat. No. Price Promo Price

White 479-0868P 1800,- 1260,-

Natural 479-0869P 1800,- 1260,-

Red 479-0870P 1800,- 1260,-

Pink 479-0871P 1800,- 1260,-

Yellow 479-0872P 1800,- 1260,-

Green 479-0873P 1800,- 1260,-

Blue 479-0874P 1800,- 1260,-

�� Optional 2-D matrix bar coded insert provides a unique identifier for traceability

�� Unique cap seal protects sample integrity during demanding freeze/thaw handling

�� Loctagon™ Vial Skirt provides stability in the free-standing position

�� Conical well allows precise sample retrieval

�� Lot certified RNase and DNase free, sterile and non-pyrogenic

VacuCap & VacuCap PF Vacuum Filtration DevicesInnovative bottle top filters for fast vacuum filtration of 100 ml to 5 l of aqueous solutions.

The novel VacuCap™ filter provides faster filtration and can be placed on any vacuum flask or bottle to collect filtered fluids, eliminating collection container limitations. The VacuCap is �� "#��$��&'��<=>J�� VacuCap PF includes a prefilter for hard to filter samples.

promo code

24

�� Contains the low protein binding Supor membrane (hydrophilic polyethersulphone) for superior flow rates and high throughputs

�� No need to constantly refill upper fluid reservoir – draws directly from the mixing reservoir

�� Eliminate possibility of contamination from transfer steps�� Environmentally friendly – minimal plastic waste�� Patented small design accepts a variety of collection vessels and

reduces both storage space and waste�� Supplied sterile in packs of 10

ProteaseARREST™>95% protease inhibition in a unique 100X liquid format

A family of protease inhibitor cocktails that inhibit over 95% of protease activity and are supplied as convenient, ready-to-use, 100X concentrated solutions. ProteaseARREST™ outperform protease inhibitor tablet format cocktails by ~80%. In addition, the unique solution format allows for instant inhibition and can be used in small sample volumes. ProteaseARREST™ is suitable for inhibition in animal tissues, plants, yeast and bacteria. Species specific and 2-D electrophoresis compatible ProteaseARREST™ cocktails are also available.

Available in multiple formats that require no dissolving or resuspension. OneQuant™ ProteaseARREST™ are a single use aliquots suitable for >95% protease inhibition in 10 ml solutions.

promo code

25

Description Pore size (μm)

Diameter (mm)

Cat. No. Price Promo price

VacuCap 60 vacuum filtration device, sterile 0.1 60 515-0016P 1225,- 795,-

0.2 60 515-0017P 1225,- 795,-

0.45 60 515-0018P 1225,- 795,-

VacuCap 60 vacuum filtration device, sterile (supplied with individually attached tubing for each filter device)

0.2 60 516-9813P 2150,- 1395,-

VacuCap 90 vacuum filtration device, sterile 0.1 90 515-0012P 1350,- 880,-

0.2 90 515-0013P 1300,- 845,-

0.45 90 515-0014P 1300,- 845,-

VacuCap 90 vacuum filtration device, sterile (supplied with individually attached tubing for each filter device)

0.2 90 516-9812P 2250,- 1465,-

0.45 90 515-0155P 2200,- 1430,-

VacuCap 60 PF vacuum filtration device, sterile 0.8/0.2 60 515-0019P 1150,- 750,-

VacuCap 90 PF vacuum filtration device, sterile 0.8/0.2 90 515-0015P 1450,- 945,-

tion in a unique

Buy one, get

one free

prtr

��

Introductory offer: 30% OFF

Introductory offer: 30% OFF

Cayman celebrates 30 years of being your source for bioactive lipids. Cayman has unparalleled expertise in: �� Synthesising high purity lipids�� Measuring bioactive lipids�� Regulating pathway enzymes�� Modulating lipid receptors

promo code

22

Product Cat. No. Price Promo price

Prostaglandin E2 EIA Kit - Monoclonal CAYM514010-96P 6675,- 4005,-

Prostaglandin E2 Express EIA Kit CAYM500141-96P 6675,- 4005,-

Prostaglandin E2 FPIA Kit - Green CAYM500501-384P 3200,- 1920,-

Prostaglandin E2 FPIA Kit - Red CAYM10004517-384P 6075,- 3645,-

Luminex© Prostaglandin E2 Kit CAYM10007501-1P 8250,- 4950,-

Luminex© Prostaglandin E2/IL-1ß Duplex Kit

CAYM10009597-1P 9525,- 5715,-

Prostaglandin E Metabolite EIA Kit CAYM514531-96P 5925,- 3555,-

Prostaglandin Screening EIA Kit CAYM514012-96P 5400,- 3240,-

*Or receive a free trial kit through the Cayman Challenge! Contact your VWR representative for details!

Discover Cayman quality! With 30 years of experience in developing methods for quantifying lipids, like PGE2, guarantees you the accuracy, sensitivity and reliability you seek. Find the format for you in our diverse collection of kits for measuring PGE2, its metabolites and related products.

CryoELITE™ 2 ml cryogenic vial with coloured cap, 500/pack

Description Pack (ml) Cat. No. Price

ProteaseARRESTTM [100X] 2 733-1996P 1600,-

OneQuant™ ProteaseARREST™ [100X] 24 x 100 μl Each 733-1944P 1650,-

FOCUS™ ProteaseARREST™ [100X] (2-D compatible)

1 733-1945P 1675,-

Recom ProteaseARREST™ [100X] (For recombinant protein protection)

1 733-1997P 1575,-

Bacterial ProteaseARREST™ [100X] 2 733-1946P 1650,-

Mammalian ProteaseARREST™ [100X] 2 733-1947P 1650,-

Plant ProteaseARREST™ [100X] 2 733-1948P 1650,-

Yeast/Fungal ProteaseARREST™ [100X] 2 733-1949P 1650,-

Save 40% on any

Cayman PGE assay kit*

Special Offer –

35% discount from

list price.

Request a FREE sample pack: Email [email protected]



ISSUE 16 - APRIL 2010��

Thermo Scientific Nalgene labtop coolersNo more messy ice baths: keep your reagents and samples cold and clean on the bench!

The Thermo Scientific Nalgene 0 °C and -20 °C Labtop Coolers will keep your temperature sensitive enzymes, reagents and samples cold on the lab bench or in the LF cabinet. The -20 °C coolers will keep microcentrifuge tubes and cryogenic vials at a temperature below -15 °C for up to 2 hours. The 0 °C coolers are designed to maintain similar at a temperature below 1 °C for 4 hours. The 0 °C labtop coolers for 10 -15 ml collection tubes will maintain this temperature for up to 5 hours, ideal for transporting clinical samples, or keeping 15 ml conical tubes cold during your experiment.

�� Just keep the coolers in a -20 °C freezer until needed�� Easy to clean - wipe them off with 70 % alcohol prior to

introducing them into an aseptic environment�� 2 microtube capacities - either 12 or 32 microtubes

ranging from 0.2 to 2 ml (adapters included)

Supporting products

promo code

27CoolCell -1 °C/minute cell freezing without alcohol!

Very innovative product for your laboratory.Achieve a consistent -1 °C/min freeze rate without the use of hazardous solvents.

promo code

28

promo codeCell

minute cell freezingut alcohol!

vative product for youra consistent -1 °C/minhe use of hazardous

28

ur laboratory.n freeze rate

s solvents.

Environmentally-friendly packaging for ART® barrier tipsThe new Molecular BioProducts ART barrier tip reload system allows 60% less waste by combining 96 tip inserts individually packaged in a blister tub made of recycled plastic, and new hinged racks.

�� The only Aerosol Barrier Tip available that guarantees 100% protection against aerosol and liquid contamination of your sample and pipette�� New racks with reversible hinged cover and one handed opening�� Racks contain a recycle logo moulded into all rack components �� The push down of blister tub easily inserts the reload into the hinged rack�� Robust insert resists flexing and bowing when seating tips on multichannels�� Ink jetted lot and part number on each 96 insert for immediate traceability�� Racks and Reloads certified free of RNase, DNase, ATP and Pyrogens

* * For use with Eppendorf pipettes and other brands with similar tip-cone fit

RNase-Away decontaminantMolecular BioProducts decontaminants provide a safe, fast, and proven method to remove nuclease and DNA contamination from bench tops and instruments, pipettes, glass and plastic ware. For your sensitive assays, RNase-Away wipes are easy-to-use on any surface and offer improved security against contamination.

promo code

26

Description Qty/Pk Code List price Promo *

RNase-Away individually wrapped wipes 35 115-1251 455,- Free*

*1 box FREE for 2 packs of Hinged Racks ART tips ordered

Description Cat. No. Price Price each for 2

CoolCell 479-0492P 830,- 580,-

�� Eliminates the expense and disposal of isopropanol

�� No stuck lids, no frozen fingers, no maintenance

�� Transportable without cold hands when frozen

�� Unbreakable even at cryogenic temperatures

Order from the Customer Service Centre or your VWR Primary Distributor:These offers cannot be used in conjunction with any other pricing agreements from VWR Interntional. Prices exclude VAT.

VWR International ASHaavard Martinsensvei 300978 Oslo

Place your order today!Tel: 02290Fax 815 00 940http://no.vwr.com

Description Packaging Cat. No. Price Cat. No. List price Promo price*

Hinged Racks

Reload Inserts

ART 10, 10 μl 10 x 96 tips 732-1139P 985,- 732-1147 890,- Free*

ART 10 REACH, 10 μl 10 x 96 tips 732-1140P 985,- 732-1148 890,- Free*

ART 20E ** 10 μl 10 x 96 tips 732-1141P 985,- 732-1149 890,- Free*

ART 20P, 20 μl 10 x 96 tips 732-1142P 985,- 732-1150 890,- Free*


ART 100E**, 100 μl 10 x 96 tips 732-1144P 985,- 732-1152 890,- Free*


ART 1000XL, 1000 μl 8 x 96 tips 732-1146P 980,- 732-1154 885,- Free*

*1 pack of Reload Inserts FREE for 2 packs of Hinged Racks ordered (same tip model)

Get 40 % off

Temperature of Nalgene® laptop cooler No. of tubes Tube size (max)

Cat. No. Price Promo price*

-20 °C 3 x 4 0.2 – 2 ml 471-0000P 1350,- 810,-

4 x 8 0.2 – 2 ml 471-0001P 2600,- 1560,-

0 °C 3 x 4 0.2 – 2 ml 479-3460P 1375,- 825,-

4 x 8 0.2 – 2 ml 479-3461P 2625,- 1575,-

3 x 4 (Ø 12-13 mm) 13 x 100 mm 471-0002P 2900,- 1740,-

3 x 4 (Ø 16-17 mm) 17 x 125 mm 471-0003P 2900,- 1740,-

g p

Get 40 % off

Buy 2 packs of Hinged Rack ART

tips and get 1 pack of Reload Inserts FREE + 1 pack of

RNase-Away wipes FREE

Buy 2, save 30%

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